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Search Results: 1 - 10 of 12998 matches for " Marilú Martins; "
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Possibilidades e desafios da produ??o científica no campo da psicologia: algumas reflex?es
Palmieri, Marilícia Witzler Antunes Ribeiro;Martins, Jo?o Batista;
Psicologia em Estudo , 2008, DOI: 10.1590/S1413-73722008000400012
Abstract: psychology scientific production indicates the existence of methodological and epistemological controversies. the emergency of new epistemological perspectives requires a discussion on the possibilities and the challenges of the knowledge construction process. discussion ranges from the acknowledgement of subjectivity in the process of scientific investigation, which, in fact, underpins the overcoming of dichotomical trends in the understanding of psi phenomena, and subverts the positivist organization of thought. since new ways for knowledge building are suggested, new concepts are provided as subjectivity factors. the researcher's labor becomes closer to that of the bricoleur's. implications in knowledge production are acknowledged and tension fields are created that facilitate a new understanding of the world. the process of knowledge construction organizes and assures a more complex glance on psi phenomena.
Criopreserva??o de ovócitos de bovinos imaturos desnudados ou n?o, utilizando o etilenoglicol pelo método da vitrifica??o
Costa, Eduardo Paulino da;Guimar?es, José Domingos;Torres, Ciro Alexandre Alves;Fagundes, Letícia Martins;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2002, DOI: 10.1590/S1516-35982002000500008
Abstract: the objective was to evaluate the effects of vitrification of immature bovine oocytes after in vitro culture, by the use of cryoprotectors ethylene glycol. oocytes from cows ovaries from slaughters houses were randomly alocated into three treatments. treatment 0 (control): frozen-thawed undesnude oocytes; treatment 1, immature vitrificated undesnude oocytes dehydrated for 5 minutes in each of the following solutions of 20, 20 and 40% of ethylene glycol, respectively, associated to 0.3 mol l-1 of trehalose and 20% of pvp, in media talp hepes, and, treatment 2, the same as treatment 1, but desnudes oocytes. after frozen-thawed of the oocytes (imersion in water bath at 30oc for 20 seconds), the oocytes were gradually rehydrated, in the following sequence of solutions: media talp hepes with 20% of ethylene glycol + 0.3 mol l-1 of trehalose + 10% of pvp and media talp hepes without ethylene glycol, trehalose and pvp, were washed three times. ultimately, the oocytes were cultured at 38.5oc, with 95% umidity and atmosphere of 5% of co2 for 24 hours. after culture, the oocytes were fertilized and the embryos cultured in vitro for seven days. the nuclear maturation were 81 (68/84), 19 (7/36) and 0% (0/31), for treatments 0, 1 and 2, respectively. the cleavage and development rates were: 56.4(102/181) and 54,9% (56/102), 1,7. (1/60) and 0,0% (1/60), 0,0 (0/71) and 0,0% (0/71), for the treatments 1, 2 e 3, respectively. these results show that the vitrification procedures, by the used protocols, are not indicated for bovine oocytes cryopreservation.
Glutamina e turnover do carbono no tecido adiposo de leit?es
Caldara, Fabiana Ribeiro;Ducatti, Carlos;Berto, Dirlei Antonio;Denadai, Juliana Célia;Andrade, Gustavo Augusto;Gioso, Marilú Martins;
Pesquisa Agropecuária Brasileira , 2007, DOI: 10.1590/S0100-204X2007001100012
Abstract: the objective of this work was to verify the glutamine influence on carbon turnover of adipose tissue of piglets. forty piglets were weaned on the 21st day, and were distributed, at random, into two treatments: t1, diet of c3 plants, without glutamine supplementation; and t2, diet of c3 plants, supplemented with 1% glutamine. on days 0, 1, 3, 5, 8, 11, 15, 20, 29 and 46 postweaning, two piglets by treatment were slaughtered; samples of the adipose tissue were collected and analyzed as for their carbon isotopic composition, and carbon substitution was measured, in function of time. in the first week postweaning, there was no alteration of the isotopic values, because the animals in this phase used their fat corporal reserve. after this period, the adipose tissue began to incorporate the isotopic signal of the ration, which indicates the onset of body fat deposition. in animals supplemented with glutamine, carbon incorporation in the fatty tissue was faster. fat reserve acquired during the suckling period have an important role in the maintenance of the animals, during approximately one week postweaning; and glutamine helps in body fat deposition retaking after this period.
Vitrifica??o de ovócitos desnudados ou n?o e previamente maturados in vitro
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Silva, Trícia Osório da;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000500004
Abstract: this study aimed at the evaluation of the effects from cryopreservation of bovine oocytes in vitro matured, by using ethylene glycol (eg) associated to trehalose and polyvinylpyrrolidone (pvp), of ovary oocytes of slaughtered cows, randomly assigned to three treatments. treatment 0 (t0 - control): oocytes that were desnuded and not vitrified. treatment 1 (t1): cryopreservation of in vitro matured oocytes with cumulus oophorus. tratamento 2 (t2): cryopreservation of in vitro matured desnuded oocytes. the percentage of recovered oocytes after cryopreservation and with normal morphology was different for vitrified oocytes (94.7 and 76.8%; 69.5 and 48.85% for t1 and t2, respectively). the main changes ultrastructural in vitrificated oocytes were prematurely released of cortical granules. later, all normal oocytes were fecundated and cultivated at 38.5oc in atmosphere with 5% co2 for seven days. the fecundation and cleavage rates for treatments were different (56.2, 41.7 and 12.5%; 36.3, 0.0 and 0.0%, for t0, t1 and t2, respectively). morulas and blastocysts were obtained only in t0 (34.5%). these results indicate that, the used protocols, for vitrification procedure is not indicated for cryopreservation of matured bovine oocytes.
Efeito de extratos de própolis verde sobre bactérias patogênicas isoladas do leite de vacas com mastite
PINTO, Marcelo Souza;FARIA, José Eurico de;MESSAGE, Dejair;CASSINI, Sérvio Túlio Alves;PEREIRA, Carmen Silva;GIOSO, Marilú Martins;
Brazilian Journal of Veterinary Research and Animal Science , 2001, DOI: 10.1590/S1413-95962001000600006
Abstract: in vitro, the sensitivity to different propolis extracts, at a concentration of 100 mg/ml, of staphylococcus aureus, staphylococcus sp. coagulase negative, streptococcus agalactiae and bacteria of the coliform group, isolated from the milk of cows with mastitis, was evaluated using the technique of an agar disk diffusion with a medium doublelayer. the results showed that the commercial propolis, the ethanolic extract, and, in a minor proportion, the methanolic extract inhibited the growth of the gram positive bacteria, staphylococcus aureus, staphylococcus sp. coagulase negative and streptococcus agalactiae. the extracts obtained through water, etila acetate and chloroform did not inhibit any bacterial strains, nor did the pure ethanol and methanol vehicles that were utilized as controls. the gram negative bacterium tested, from the coliform group, did not show sensitivity to any extract. bacterial strains of the same species collected from different sources presented significant differences in sensitivity to the extracts (p < 0.05). in the streptococcus agalactiae samples, the diameters of the zone of inhibition around the disks were bigger than those observed for samples of staphylococcus aureus and staphylococcus sp. coagulase negative. the results of this experiment stimulate the continuation of studies on the use of propolis extracts, by means of using the appropriate vehicles for the treatment of bovine mastitis.
Doen?a celíaca e infertilidade feminina: associa??o freqüentemente negligenciada
Martins, Carmen Lívia da Silva;Gandolfi, Lenora;Tauil, Pedro Luiz;Pican?o, Marilúcia de Almeida Rocha;Araujo, Maria Ophelia Galv?o de;Pratesi, Riccardo;
Revista Brasileira de Ginecologia e Obstetrícia , 2006, DOI: 10.1590/S0100-72032006001000006
Abstract: purpose: to verify the existence of association between sub-fertility or infertility and concurrent celiac disease in women attended at a reproductive disorders out-patient clinic of a general hospital. methods: this was a case-control study in which a group of 200 women with difficulty in conceiving was compared with a control group with 400 women who were treated at the same hospital for various reasons, but not for fertility problems. both groups were submitted to the anti-endomysium antibody protocol using the indirect immunofluorescence method. whenever positive, the diagnosis was further confirmed by endoscopic duodenal biopsy and histopathological exams. results: anti-endomysium testing resulted positive in three patients (1.5%) within the group of women with difficulty in conceiving. diagnosis of celiac disease was subsequently confirmed by histopathological exams of the duodenal mucosa. all the control patients presented negative results in the serological exams. conclusion: celiac disease was observed only in women with difficulty in conceiving, a relevant fact indicating that this disease may be more common in infertile women. however, new studies with a greater sample size are needed to confirm this possible association.
Perfil de progesterona e intervalo ao estro de receptoras bovinas sincronizadas com doses reduzidas de cloprostenol
Gioso, Marilú Martins;Costa, Eduardo Paulino da;Fernandes, Carlos Ant?nio de Carvalho;Torres, Ciro Alexandre Alves;Carvalho, Giovanni Ribeiro de;
Revista Brasileira de Zootecnia , 2005, DOI: 10.1590/S1516-35982005000400012
Abstract: the objective of this research was to compare the effects of different doses of application of sodic cloprostenol (doses equivalent to 50% the conventional), in the intramuscular (im) and intra-vulvosubmucosal (ivsm) vias on the synchronization rates, interval of application to estrus and decrease of seric progesterone concentrations. one hundred and ninety-nine females, 103 with of previus report estrous and 96 without report of previus estores evaluated by rectal palpation to verify the presence of corpus luteum, were randomly divided into three treatments: 1 - 68 animals (38 with report of previous estrus) received 1 ml of cloprostenol through ivsm; 2 - 66 animals (33 with report of previous estrus) received 1 ml through im; 3 - 65 animals (32 with report of previous estrus) received 2 ml through im. it was considered the animals that showed estrus up to 96 hours after cloprostenol application. the seric progesterone concentrations (p4) were analyzed by ria at 0 and 48 hours after the application of luteolitic drug. no effects of treatments on synchronization rates (72.1, 53.0, and 64.6%, respectively), interval from application to estrus (69.3 ± 15.2, 67.9 ± 16.7, and 68.3 ± 16.9 hours, respectively) and p4 percentage concentration decrease (79; 68 and 83%, respectively) were observed. however, when data of animals from treatments of days 11 to 16 were grouped and compared to those of treatments of days 7 to 10, the synchronization rates differed (76.1% vs 55.5%). sodic cloprostenol doses that was reduced by 50%, in im or ivsm vias, showed effect similar to the conventional dose. animals during days 11 to 16 respond better to a synchronization with cloprostenol than those during days 7 to 10 of diestrus.
Rotavirus e adenovirus em crian?as de 0-5 anos hospitalizadas com ou sem gastrenterite em Goiania - GO., Brasil
Cardoso, Divina das Dores de Paula;Martins, Regina Maria Bringel;Kitajima, Eliott W.;Barbosa, Aristides José;Camarota, Sandra Cristina Teles;Azevedo, Maril Silva Pereira;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1992, DOI: 10.1590/S0036-46651992000500010
Abstract: in order to detect rotavirus and adenovirus 557 feces samples from hospitalized children (0-5 years of age) were analysed from june 1987 to july 1990 in goiania-city.two hundred and ninety one samples were from children with diarrhoea and 266 were from children without diarrhoea. amongst this later group, 64 samples were from children from the nursery. two hundred and sixty one out of 557 samples were analysed by immunoelectron microscopy (iem), polyacrylamide gel electrophoresis (sds-page) and enzymatic immunoassay for rotavirus and adenovirus (eiara) whereas the rest (296 samples) were analysed by sds-page and eiara. positivity of rotavirus and adenovirus was 17.2% and 2.1% respectively. concerning rotavirus and adenovirus there was 29.2% and 2.4% positivity within the group with diarrhoea and 4.1% and 1.5% positivity amongst children without diarrhoea (p<0.05). rotavirus were more prevalent amongst children which age ranged from 1 to 11 months of age. no newborn child from the nursery was positive for rotavirus. adenovirus were detected amongst children from 1 to 3 years of age. rotavirus circulation peak occurred between may and august (p<0.05) and no positive case was detected from december to february. two hundred out of 291 diarrheic samples were also studied concerning bacteria and pathogenic parasites and equal percentages (17.0%) were found for both rotavirus and pathogenic bacteria. eighty nine samples of rotavirus were detected by sds-page and 86 of these (96.6%) belonged to the subgroup ii with 13 different electrophoretic patterns. predominance of a given eletropherotic profile was observed in each year of the study.
Febre maculosa no município de Pedreira, SP, Brasil. Inquérito sorológico
Del Guercio, Vania Martins Fontes;Rocha, Marilú Mendes M.;Melles, Heloísa H.B.;Lima, Virgília C.L. de;Pignatti, Marta G.;
Revista da Sociedade Brasileira de Medicina Tropical , 1997, DOI: 10.1590/S0037-86821997000100009
Abstract: cases of brasilian spotted fever (bsf) have been occurred since 1985, in the rural area of pedreira town, situated 160km away from the city of s?o paulo (longitude 46o54'07"; latitude 22o44'21"). aiming at evaluating the prevalence of brasilian spotted fever in this endemic area, single-serum samples were collected from 473 healthy persons, amongst city-dwellers and the local china industry workers. the obtained samples were tested by indirect immunofluorescence (ifa), in order to determine the antibodies titer for the group of brasilian spotted fever. twenty-five (5.3%) were considered positive (titer 3 1:64) and thirty-one (6.5%) were taken as "borderline" (titer 1:32). the results show a serologically positivity rate similar to other areas, known as endemic ones within the country.
Febre maculosa no município de Pedreira, SP, Brasil. Inquérito sorológico
Del Guercio Vania Martins Fontes,Rocha Marilú Mendes M.,Melles Heloísa H.B.,Lima Virgília C.L. de
Revista da Sociedade Brasileira de Medicina Tropical , 1997,
Abstract: Casos de febre maculosa brasileira vêm ocorrendo desde 1985 no município de Pedreira. Com o objetivo de avaliar a prevalência da febre maculosa brasileira nessa área endêmica, foram coletadas amostras únicas de soro de 473 pessoas sadias, moradores e funcionários de uma indústria de lou as. As amostras obtidas foram testadas através da rea o de imunofluorescência indireta (IFA), para determina o do título de anticorpos para ricketttsia do grupo da febre maculosa brasileira. Vinte e cinco (5,3%) foram considerados positivos (título superscript three1:64) e trinta e um (6,5%) apresentaram título igual a 1:32 ("borderline"). Os resultados evidenciam uma taxa de soropositividade semelhante a outras áreas reconhecidamente endêmicas do país.
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