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Search Results: 1 - 10 of 332775 matches for " Marie S Ostenfeld "
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Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs
Steffen G Jensen, Philippe Lamy, Mads H Rasmussen, Marie S Ostenfeld, Lars Dyrskj?t, Torben F ?rntoft, Claus L Andersen
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-435
Abstract: Using synthetic miRNA samples and plasma RNA samples spiked with different ratios of 174 synthetic miRNAs we assessed the performance characteristics reproducibility, recovery, specificity, sensitivity and linearity. It was found that while the qRT-PCR based platforms were sufficiently sensitive to reproducibly detect miRNAs at the abundance levels found in human plasma, the array based platform was not. At high miRNA levels both qRT-PCR based platforms performed well in terms of specificity, reproducibility and recovery. At low miRNA levels, as in plasma, the miRCURY platform showed better sensitivity and linearity than the TaqMan platform.For profiling clinical samples with low miRNA abundance, such as plasma samples, the miRCURY platform with its better sensitivity and linearity would probably be superior.microRNAs (miRNAs) are short 20-23 nucleotide long non-coding RNAs that are widely distributed in almost all eukaryotic organisms. They have multiple functions however the main function is believed to be post transcriptional regulation of protein levels [1,2]. While miRNAs are often abundant in tissues, the amount found circulating in body fluids such as plasma and serum is often limited. It has been reported that the total RNA level in plasma is in the range 6-300 ng/ml [3,4] and that the miRNA fraction constitutes only a few percent of this [5]. The mechanisms regulating secretion of miRNA into circulation is still unclear. Reports have shown that while endogenous miRNAs appear stable in plasma/serum exogenous miRNAs are not, and as a result of this it has been suggested that endogenous circulating miRNAs are either encapsulated in microvesicles or bound to RNA-binding proteins in complexes, e.g. Ago2 and NPM1, protecting them from degradation [6-8]. Detailed knowledge of the biological function of circulating miRNA does not exist, however it has been shown that vesicular miRNAs can be transferred from cell to cell and influence the behavior of the recipient c
Functional Screening Identifies miRNAs Influencing Apoptosis and Proliferation in Colorectal Cancer
Lise Lotte Christensen, Anja Holm, Juha Rantala, Olli Kallioniemi, Mads H. Rasmussen, Marie S. Ostenfeld, Frederik Dagnaes-Hansen, Bodil ?ster, Troels Schepeler, Heidi Tobiasen, Kasper Thorsen, Oliver M. Sieber, Peter Gibbs, Philippe Lamy, Torben F. Hansen, Anders Jakobsen, Eva M. Riising, Kristian Helin, Jan Lubinski, Rikke Hagemann-Madsen, S?ren Laurberg, Torben F. ?rntoft, Claus L. Andersen
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096767
Abstract: MicroRNAs (miRNAs) play a critical role in many biological processes and are aberrantly expressed in human cancers. Particular miRNAs function either as tumor suppressors or oncogenes and appear to have diagnostic and prognostic significance. Although numerous miRNAs are dys-regulated in colorectal cancer (CRC) only a small fraction has been characterized functionally. Using high-throughput functional screening and miRNA profiling of clinical samples the present study aims at identifying miRNAs important for the control of cellular growth and/or apoptosis in CRC. The high-throughput functional screening was carried out in six CRC cell lines transfected with a pre-miR library including 319 synthetic human pre-miRs. Phenotypic alterations were evaluated by immunostaining of cleaved cPARP (apoptosis) or MKI67 (proliferation). Additionally, TaqMan Human MicroRNA Array Set v2.0 was used to profile the expression of 667 miRNAs in 14 normal colon mucosa and 46 microsatellite stable stage II CRC patients. Among the miRNAs that induced growth arrest and apoptosis in the CRC cell lines, and at same time were dys-regulated in the clinical samples, miR-375 was selected for further analysis. Independent in vitro analysis of transient and stable transfected CRC cell lines confirmed that miR-375 reduces cell viability through the induction of apoptotic death. We identified YAP1 as a direct miR-375 target in CRC and show that HELLS and NOLC1 are down-stream targets. Knock-down of YAP1 mimicked the phenotype induced by miR-375 over-expression indicating that miR-375 most likely exerts its pro-apoptotic role through YAP1 and its anti-apoptotic down-stream targets BIRC5 and BCL2L1. Finally, in vivo analysis of mouse xenograft tumors showed that miR-375 expression significantly reduced tumor growth. We conclude that the high-throughput screening successfully identified miRNAs that induce apoptosis and/or inhibit proliferation in CRC cells. Finally, combining the functional screening with profiling of CRC tissue samples we identified clinically relevant miRNAs and miRNA targets in CRC.
Comparative Chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357)
Christian Rank,Marie Louise Klejnstrup,Lene Maj Petersen,Sara Kildgaard,Jens Christian Frisvad,Charlotte Held Gotfredsen,Thomas Ostenfeld Larsen
Metabolites , 2012, DOI: 10.3390/metabo2010039
Abstract: Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions. For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.
Aspergillus nidulans Synthesize Insect Juvenile Hormones upon Expression of a Heterologous Regulatory Protein and in Response to Grazing by Drosophila melanogaster Larvae
Morten Thrane Nielsen, Marie Louise Klejnstrup, Marko Rohlfs, Diana Chinyere Anyaogu, Jakob Bl?sbjerg Nielsen, Charlotte Held Gotfredsen, Mikael R?rdam Andersen, Bjarne Gram Hansen, Uffe Hasbro Mortensen, Thomas Ostenfeld Larsen
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0073369
Abstract: Secondary metabolites are known to serve a wide range of specialized functions including communication, developmental control and defense. Genome sequencing of several fungal model species revealed that the majority of predicted secondary metabolite related genes are silent in laboratory strains, indicating that fungal secondary metabolites remain an underexplored resource of bioactive molecules. In this study, we combine heterologous expression of regulatory proteins in Aspergillus nidulans with systematic variation of growth conditions and observe induced synthesis of insect juvenile hormone-III and methyl farnesoate. Both compounds are sesquiterpenes belonging to the juvenile hormone class. Juvenile hormones regulate developmental and metabolic processes in insects and crustaceans, but have not previously been reported as fungal metabolites. We found that feeding by Drosophila melanogaster larvae induced synthesis of juvenile hormone in A. nidulans indicating a possible role of juvenile hormone biosynthesis in affecting fungal-insect antagonisms.
Expression of Myoglobin in the Urine of Cocaine Users  [PDF]
Marie M. Bourgeois, Ira S. Richards
International Journal of Clinical Medicine (IJCM) , 2013, DOI: 10.4236/ijcm.2013.44038
Background: The possibility exists that cocaine use in the absence of frank clinical presentation of acute cardiac events may be associated with subclinical injury including the release of myoglobin which is a nonspecific marker for cardiac and skeletal muscle damage. Objectives: This investigation examined urine specimens for potential differences between cocaine use and the expression of myoglobin (a marker associated with cardiovascular damage, inflammation and oxidative stress). Methods:40 urine specimens were assayed for cocaine metabolites, creatinine, total protein (BSA) and myoglobin using ELISA and colorimetry. Results: We observed significant differences between male control and male cocaine positive urines for myoglobin. Interestingly, there was no statistically significant difference in females between control and cocaine positive urines. Conclusion: Differences in the urinary expression of myoglobin may be important in evaluating the gender based effects of cocaine use and may have potential clinical applications which may be related to gender differences in signs and symptoms of cocaine toxicity.
Une étude du pluralisme architectural: l'indicateur des métamorphoses du quartier Mariscal Sucre à Quito
Marie S. BOCK
Mappemonde , 1993,
Abstract: L’étude de la carte du pluralisme architectural du quartier Mariscal Sucre à Quito, élaborée à partir d’une matrice de Bertin, permet de mettre en valeur une dichotomie chronologique et fonctionnelle et de dégager des profils architecturaux soulignant les différentes étapes de constitution et d’évolution de ce secteur.
Espace et société dans les départements d'outre-mer
Marie S. Bock
Mappemonde , 1999,
Abstract: Des débuts de la colonisation jusqu'au milieu du xixe siècle, les actuels départements insulaires sont soumis à une économie de cycles fondée sur l'exportation de produits tropicaux; à partir des années 1960, les transferts financiers prennent le relais. La grille spatio-temporelle présente la succession des cycles économiques et prend en compte les évolutions des structures sociales. En effet, le recours à une main-d'uvre asservie durant la période coloniale a laissé un lourd héritage aux sociétés domiennes.
YKL-40 tissue expression and plasma levels in patients with ovarian cancer
Estrid VS H?gdall, Merete Ringsholt, Claus K H?gdall, Ib Jarle Christensen, Julia S Johansen, Susanne K Kjaer, Jan Blaakaer, Lene Ostenfeld-M?ller, Paul A Price, Lise H Christensen
BMC Cancer , 2009, DOI: 10.1186/1471-2407-9-8
Abstract: YKL-40 protein expression was determined by immunohistochemistry in tissue arrays from 181 borderline tumors and 473 OC. Plasma YKL-40 was determined by ELISA in preoperative samples from 19 patients with borderline tumor and 76 OC patients.YKL-40 protein expression was found in cancer cells, tumor associated macrophages, neutrophils and mast cells. The tumor cell expression was higher in OC than in borderline tumors (p = 0.001), and associated with FIGO stage (p < 0.0001) and histological subtype (p = 0.0009). Positive YKL-40 expression (≥ 5% staining) was not associated with reduced survival. Plasma YKL-40 was also higher in patients with OC than in patients with borderline tumors (p < 0.0001), and it was positively correlated to serum CA-125 (p < 0.0001) and FIGO stage (p = 0.0001). Univariate Cox analysis of plasma YKL-40 showed association with overall survival (p < 0.0001). Multivariate Cox analysis, including plasma YKL-40, serum CA125, FIGO stage, age and radicality after primary surgery as variables, showed that elevated plasma YKL-40 was associated with a shorter survival (HR = 2.13, 95% CI: 1.40–3.25, p = 0.0004).YKL-40 in OC tissue and plasma are related to stage and histology, but only plasma YKL-40 is a prognostic biomarker in patients with OC.YKL-40 (chitinase-3-like-1) is a highly conserved protein [1] and a member of "mammalian chitinase-like proteins" [1,2]. The protein is expressed in many types of cancer cells (dbest NCBI database), and elevated plasma levels are predictive of poor prognosis in patients with different types of cancer [2-8]. The highest plasma YKL-40 levels have been found in patients with metastatic disease, short recurrence/progression-free intervals, and short overall survival [2-8]. Furthermore, plasma YKL-40 has provided independent information on prognosis over clinical characteristics and biomarkers, such as serum CA-125, LDH, PSA, CEA, and HER2 [2-8]. It has been suggested that YKL-40 is associated with cancer cell prolife
Ethnicity and Fatigue: Expressions of Distress, Causal Attributions and Coping  [PDF]
Kamaldeep S Bhui, Sokratis Dinos, Marie-Laure Morelli
Sociology Mind (SM) , 2011, DOI: 10.4236/sm.2011.14020
Abstract: This paper reports on an MRC funded study of chronic fatigue and ethnicity. The purpose of the qualitative component was to compare expressions of fatigue, illness attribution, coping styles and help seeking behaviour across ethnic groups. The study used secondary analysis of qualitative data that were collected as part of the Ethnic Minority Psychiatric Illness Rates in the Community (EMPIRIC) study. The charts of the qualitative data used in the original study and the original transcripts were used to identify reports of fatigue and related symptoms that included feeling tired, exhausted, shattered, lack of energy, lack of sleep, insomnia and impaired concentration. We described symptoms by ethnic group, and examined whether ethnicity influenced illness attributions, coping strategies and help seeking behaviour. Fatigue related symptoms were common and encountered in all the ethnic groups studied, and descriptions of fatigue, illness attribution and help seeking, on the whole, did not vary between ethnic groups. The paper sets out the subtle differences between ethnic groups, and considers the use of secondary qualitative data analysis in research. Fatigue was more common among women; coping included self-help, and seeking help from social and counselling services. Religion was used as a coping mechanism mainly amongst the Pakistani, Indian and Bangladeshi ethnic groups.
Experimental Investigations of the Shear Capacity of Nails in a Row  [PDF]
Christian O. S?rensen, Ruth-Marie Nymark, Lars Baastad
Open Journal of Civil Engineering (OJCE) , 2013, DOI: 10.4236/ojce.2013.33021

Tests of the capacity of shear connections consisting of nails in a row placed at distances 7, 10 and 14d, “d” being the cross-sectional dimension of the nail, versus single nail capacities, were executed. The performed tests do support the connotation that no reduction should be required for nails of diameter 2.8 mm or less in a row, provided that nails are spaced sufficiently far apart for wood cracking not to occur. At the ultimate capacity of the joint, all such thin nails in a row will be yielding, having developed plastic hinges, i.e. each single nail will have developed its ultimate capacity. Hence, the ultimate capacity of the connection will be each nail’s capacity times the number of nails in the row. The force pr. nail increases subsequent to the development of a plastic hinge. This is likely due to the axial pullout-force, i.e. the ultimate capacity of a shear connection is higher than the force required for developing plastic hinges in the nails. This additional capacity-reserve may also partly be attributed to the rotational resistance of nails. The number of nails in a row should make insignificant difference in the pr. nail capacity, as long as no wood cracking takes place. Thus, applying elastic theory to nails in a row does not seem relevant. This is in contrast to bolt-connections.

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