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Search Results: 1 - 10 of 223161 matches for " Maria de Lourdes RS Cunha "
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Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci
Adilson Oliveira, Maria de Lourdes RS Cunha
BMC Research Notes , 2010, DOI: 10.1186/1756-0500-3-260
Abstract: Among the 100 CNS isolates studied, 82% tested positive by PCR, 82% by the tube test, 81% by the TCP assay, and 73% by the CRA method. Using PCR as a reference, the tube test showed the best correlation with detection of the ica genes, presenting high sensitivity and specificity.The tube adherence test can be indicated for the routine detection of biofilm production in CNS because of its easy application and low cost and because it guarantees reliable results with excellent sensitivity and specificity.Coagulase-negative staphylococci (CNS) are the microorganisms most frequently involved in nosocomial infections among neonates. These infections are generally associated with the use of catheters and other medical devices [1]. The capacity to adhere to polymer surfaces and consequent biofilm production are the main virulence factors of CNS, especially S. epidermidis, the most frequently isolated species.The biofilm protects CNS against the action of antibiotics administered for the treatment of these infections and also against the patient's immune system. Removal of the foreign body is often necessary for cure [2,3]. In this respect, CNS infections seem to be related to the health condition of the patient and to the production of this extracellular polysaccharide [4,5].The biofilm consists of layers of cell clusters embedded in a matrix of extracellular polysaccharide, called polysaccharide intercellular adhesin (PIA), which consists of β-1,6-N-acetylglycosamine and is synthesized by N-acetylglucosaminyltransferase [6]. PIA is involved in cell-cell adhesion and is essential for biofilm production by CNS, which is observed in most clinical strains of S. epidermidis [7,8].The synthesis of PIA is mediated by the products of the chromosomal ica gene (intercellular adhesion), which are organized in an operon structure. This operon contains the icaADBC genes, in addition to the icaR gene which exerts a regulatory function and is transcribed in the opposite direction. Once t
Comparison of methods for the identification of coagulase-negative staphylococci
Cunha, Maria de Lourdes RS;Sinzato, Yuri K;Silveira, Liciana VA;
Memórias do Instituto Oswaldo Cruz , 2004, DOI: 10.1590/S0074-02762004000800012
Abstract: coagulase-negative staphylococci (cns) species identification is still difficult for most clinical laboratories. the scheme proposed by kloos and schleifer and modified by bannerman is the reference method used for the identification of staphylococcal species and subspecies; however, this method is relatively laborious for routine use since it requires the utilization of a large number of biochemical tests. the objective of the present study was to compare four methods, i.e., the reference method, the api staph system (biomérieux) and two methods modified from the reference method in our laboratory (simplified method and disk method), in the identification of 100 cns strains. compared to the reference method, the simplified method and disk method correctly identified 100 and 99% of the cns species, respectively, while this rate was 84% for the api staph system. inaccurate identification by the api staph method was observed for staphylococcus epidermidis (2.2%), s. hominis (25%), s. haemolyticus (37.5%), and s. warneri (47.1%). the simplified method using the simple identification scheme proposed in the present study was found to be efficient for all strains tested, with 100% sensitivity and specificity and proved to be available alternative for the identification of staphylococci, offering, higher reliability and lower cost than the currently available commercial systems. this method would be very useful in clinical microbiology laboratory, especially in places with limited resources.
Comparison between qualitative and semiquantitative catheter-tip cultures: laboratory diagnosis of catheter-related infection in newborns
Marconi, Camila;Cunha, Maria de Lourdes RS;Lyra, Jo?o C;Bentlin, Maria R;Batalha, Jackson EN;Sugizaki, Maria Fátima;Rugolo, Lígia MSS;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000200012
Abstract: this prospective study evaluated semiquantitative and qualitative catheter-culture methods for diagnosis of catheter-related infection (cri) in newborns. catheter tips from newborns admitted to the neonatal unit of the university hospital of the botucatu medical school, unesp were included in the study. catheter cultures were performed with both semiquantitative and qualitative techniques. for cri diagnosis, microorganisms isolated from catheter cultures and from peripheral blood cultures were identified and submitted to agent susceptibility test. the gold standard was the certain cri diagnosis when same microorganism (specie and profile of susceptibility to agents) was isolated from both catheter tips and peripheral blood culture. a total of 85 catheters from 63 newborns were included in the study. the semiquantitative culture method, despite presenting lower sensitivity (90%), showed higher specificity (71%) when compared to 100% of sensitivity and 60% of specificity in the qualitative method. the identification of the microorganisms obtained from the catheter cultures showed a prevalence of coagulase-negative staphylococci(cns) species. the specie staphylococcus epidermidis (77.5%) was the prevalent in the catheters with positive semiquantitative cultures. among 11 episodes with cri diagnosis, 8 (72.7%) were associated with cns species, of which 6 were s. epidermidis. two episodes of cri by s. aureus and one by candida parapsilosis were also detected. the semiquantitative catheter-culture method showed advantages for cri diagnosis in newborns when compared to the conservative qualitative method.
Significancia clínica de estafilococos coagulase-negativa isolados de recém-nascidos
RS Cunha,María de Lourdes; AM Lopes,Carlos; MSS Rugolo,Lígia; VAS Chalita,Liciana;
Revista chilena de pediatría , 2004, DOI: 10.4067/S0370-41062004000300014
Abstract: objective: to evaluate the clinical significance of coagulase-negative staphylococci (cns) isolated from newborns? infections at unidade neonatal do hospital das clínicas da faculdade de medicina de botucatu. method: the cns strains isolated were identified and classified as clinically significant and contaminant, based on a series of clinical and laboratorial data obtained of the chart of the patients who stayed in the neonatal unit. the following data were analysed: risk factors for infections, clinical evolution, abnormal blood cell counts and/or c-reative protein e antibiotic therapy. results: of the 117 strains of cns isolated, 60 (51,3%) were classified as significant and 57 (48,7%) as contaminant. of the 54 infants infected by cns, 43 (79,6%) were very low birth weight (< 1.500 g). most of the infants infected by cns were submitted to two or more procedures (77,8%), including catheter (88,9%), parenteral nutrition (64,8%) and mechanical ventilation (61,1%). staphylococcus epidermidis was the most frequently isolated species (77,8%) and more associated with infection (86,7%) than with contamination (68,4%). other species of cns, including two strains of s. haemolyticus, three strains of s. lugdunensis, one strain of s. simulans, one strain of s. warneri and one strain of s. xylosus were also isolated from infants with clinical evidence of pneumonia, necrotizing enterocolitis and sepsis. conclusion: most of newborns infected by cns presented important risk factors for the installation of the infectious process, including birth weight < 1 500 g, not removal foreign body and previous use of antimicrobials. the identification of species of ecn constitutes an useful marker of infection, since the s. epidermidis was the specie more frequently associated to the infection
Significancia clínica de estafilococos coagulase-negativa isolados de recém-nascidos Clinical significance of coagulase-negative staphylococci isolated of neonates
María de Lourdes RS Cunha,Carlos AM Lopes,Lígia MSS Rugolo,Liciana VAS Chalita
Revista chilena de pediatría , 2004,
Abstract: Objetivo: Avaliar a significancia clínica de estafilococos coagulase-negativa (ECN) isolados de processos infecciosos em recém-nascidos da Unidade Neonatal do Hospital das Clínicas da Faculdade de Medicina de Botucatu. Método: As linhagens de ECN isoladas foram identificadas e classificadas em significativas e contaminantes com base em uma série de dados clínicos e laboratoriais obtidos dos prontuários dos pacientes internados na Unidade Neonatal. Foram pesquisados os dados referentes a fatores perinatais de risco para infec o, evolu o clínica, altera es do hemograma e/ou positividade de proteína C reativa e antibioticoterapia. Resultados: Das 117 linhagens de ECN isoladas, 60 (51,3%) foram classificadas como significativas e 57 (48,7%) como contaminantes. Das 54 crian as com infec o por ECN, 43 (79,6%) eram prematuras e 27 (50,0%) com peso ao nascimento < 1 500 g. A maioria das crian as com infec o por ECN estavam submetidas a dois ou mais procedimentos invasivos (77,8%), incluindo o uso de cateter (88,9%), nutri o parenteral (64,8%) e ventila o mecanica (61,1%). OS. epidermidis foi a espécie mais frequentemente isolada (77,8%) e mais associada com infec o (86,7%) do que com contamina o (68,4%). Outras espécies de ECN, incluindo duas linhagens de S. haemolyticus, três linhagens de S. lugdunensis, uma linhagem de S. simulans, uma de S. warneri e uma linhagem de S. xylosus também foram isoladas de crian as com evidência clínica de pneumonia, enterocolite necrosante e sepse. Conclus o: A maioria dos recém-nascidos com infec o por ECN apresentou fatores predisponentes importantes para a instala o do processo infeccioso, incluindo o peso de nascimento < 1 500 g, a n o remo o de corpo estranho e a antibioticoterapia prévia. A identifica o de espécies de ECN constitui um marcador útil de infec o, visto que o S. epidermidis foi o agente etiológico mais frequentemente associado aos processos infecciosos Objective: To evaluate the clinical significance of coagulase-negative staphylococci (CNS) isolated from newborns’ infections at Unidade Neonatal do Hospital das Clínicas da Faculdade de Medicina de Botucatu. Method: The CNS strains isolated were identified and classified as clinically significant and contaminant, based on a series of clinical and laboratorial data obtained of the chart of the patients who stayed in the Neonatal Unit. The following data were analysed: risk factors for infections, clinical evolution, abnormal blood cell counts and/or C-reative protein e antibiotic therapy. Results: Of the 117 strains of CNS isolated, 60 (51,3%) were
Publicity and identity in the public space architecture
Maria de Lourdes Carneiro da Cunha Nóbrega,Clarissa Duarte
Urbe : Revista Brasileira de Gest?o Urbana , 2009,
Abstract: This article aims at showing the relationship between publicity elements, such as posters and signs, with the city architecture, so that the contribution of these elements with the identity of the urban sites, especially the historical ones, can be understood. In order to do that, a focal point is given to Rua da Palma, located in the city of Recife (Pernambuco, Brazil). For the development of this research, which was based on the present morphological analysis of the site, a survey of photographs and the use of the buildings in the street from 2006 to 2009 was carried out. Also, the existing urban legislation was analyzed. Studies undertaken by authors such as Certau (1994), Venturi (1977) and Koolhaas (2004), among others, and concepts related to retail marketing helped in the conclusion of this analysis of the urban space, which presents architecture as a publicity media, often transforming the identity of the area. A starting point is presented here for future investigation on the role of urban laws and urban control, which deal with the placement of publicity elements in the architecture of the city and contribute for the formation or the urban landscape. This landscape which is considered an integral part of a cultural identity.
Estudo da produ??o de beta -lactamase e sensibilidade às drogas em linhagens de estafilococos coagulase-negativos isolados de recém-nascidos
Cunha, Maria de Lourdes Ribeiro de Souza da;Lopes, Carlos Alberto de Magalh?es;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2002, DOI: 10.1590/S1676-24442002000400006
Abstract: although coagulase-negative staphylococci (cns) have been recognized as saprophytes for a long time, they had emerged as etiologic agents of infections. they have currently been the most frequently isolated pathogen in sepsis in neonatal intensive care unit (nicu). this study aimed the identification of cns strains isolated from newborns' infections and to determination of b-lactamase and drugs susceptibility. staphylococcus epidermidis was the most frequently isolated species (77,8%). the study of the b-lactamase production revealed this characteristic in the most of the strains of cns isolated (71,8%). the strains isolated in this study presented multiple resistance to the antibiotics tested, with 63,2% of isolates presenting resistance to five or more drugs. the high transmissibility of plasmids among those strains and the abusive use of drugs antimicrobial has been constituting in important factors to the selection of multi-resistance samples and the transfer of resistance genes.
Estudo da produ o de b -lactamase e sensibilidade às drogas em linhagens de estafilococos coagulase-negativos isolados de recém-nascidos
Cunha Maria de Lourdes Ribeiro de Souza da,Lopes Carlos Alberto de Magalh?es
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2002,
Abstract: Os estafilococos coagulase-negativos (ECN), embora reconhecidos como saprófitas por muito tempo, têm emergido como agentes etiológicos de uma série de infec es, sendo atualmente os principais responsáveis por sepse em UTI neonatal. Tendo em vista estas características, este estudo objetivou a identifica o de estafilococos coagulase-negativos isolados de processos infecciosos em recém-nascidos, bem como a determina o da produ o de beta-lactamase e sensibilidade às drogas pelas linhagens isoladas. O Staphylococcus epidermidis foi a espécie mais freqüentemente isolada (77,8%). O estudo da produ o de beta-lactamase revelou esta característica na maioria das linhagens de ECN isoladas (71,8%). As linhagens de ECN mostraram, ainda, resistência múltipla aos antibióticos utilizados, com 63,2% dos isolados apresentando resistência a cinco ou mais drogas. A elevada transmissibilidade de plasmídios entre estas linhagens e o uso abusivo de drogas antimicrobianas têm-se constituído em importantes fatores na sele o de amostras multirresistentes e na transferência de genes de resistência.
Avalia??o da coloniza??o nasal por Staphylococcus spp. resistenteà oxacilina em alunos de enfermagem
Pereira, Eliane Patricia Lino;Cunha, Maria de Lourdes Ribeiro de Souza da;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2009, DOI: 10.1590/S1676-24442009000500004
Abstract: introduction: the genus staphylococcus is responsible for a great number of bacterial infections in human, mainly in hospital environment. objectives: in view of these considerations and the importance of nursing care and nosocomial infection control, this study verified the rate of s. aureus and coagulase-negative staphylococci (cns) carriers resistant to oxacillin in nursing students during their university course. materials and methods: nasal samples were collected from nursing students at botucatu school of medicine - unesp. results: from 109 isolated samples of staphylococcus, 30 samples (27.5%) were staphylococcus aureus and 79 samples (72.5%) were cns. from 79 identified cns samples, 63 (79.7%) were s. epidermidis, nine (11.4%) s. warneri, three (3.8%) s. haemolyticus, two (2.5%) s. capitis, one (1.3%) s. simulans and one (1.3%) s. lugdunensis. the antibiotic susceptibility test showed 100% sensibility to the drugs in s. aureus samples and among 79 cns samples, 10 (12,6%) were resistant to oxacillin. the pcr technique demonstrated negative result for meca gene in s. aureus samples and 11 positive samples among cns species. discussion: there was no relation between the rate of s. aureus carriers and nosocomial involvement during the course. the results also showed a higher incidence of resistance in cns samples, which is seemingly reported in the scientific literature.
Study of virulence factors in coagulase-negative staphylococci isolated from newborns
Cunha, Maria de Lourdes Ribeiro de Souza da;Rugolo, Ligia Maria Suppo de Souza;Lopes, Carlos Alberto de Magalh?es;
Memórias do Instituto Oswaldo Cruz , 2006, DOI: 10.1590/S0074-02762006000600014
Abstract: coagulase-negative staphylococci (cns) have been identified as the etiological agent in various infections and are currently the microorganisms most frequently isolated in nosocomial infections. however, little is known about the virulence factors produced by cns that contribute to the pathogenesis of infections caused by these microorganisms. the study of cns isolated from infectious processes of newborns hospitalized in the neonatal unit of the hospital of the botucatu medical school, unesp, indicated staphylococcus epidermidis as the most frequently isolated species (77.8%), which was also associated with clinically significant situations. the analysis of virulence factors revealed the production of slime in 20 (17.1%) of all cns samples isolated and the synthesis of a broad spectrum of enzymes and toxins, including hemolysins (19.6%), lipase (17.1%), lecithinase (3.4%), dnase (15.4%), thermonuclease (7.7%), and enterotoxin a, b or c (37.6%). taking into consideration that the etiological importance of cns has often been neglected, the present investigation confirmed that these microorganisms should not be ignored or classified as mere contaminants.
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