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Search Results: 1 - 10 of 71038 matches for " Maria Ruaro "
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Characterization of the time course of changes of the evoked electrical activity in a model of a chemically-induced neuronal plasticity
Frédéric D Broccard, Silvia Pegoraro, Maria Ruaro, Claudio Altafini, Vincent Torre
BMC Research Notes , 2009, DOI: 10.1186/1756-0500-2-13
Abstract: In the present manuscript we analysed the time course of changes of the evoked electrical activity during neuronal plasticity and we correlated it with a transcriptional analysis of the underlying changes of gene expression. Our investigation shows that treatment for 30 min. with the GABAA receptor antagonist gabazine (GabT) causes a potentiation of the evoked electrical activity occurring 2–4 hours after GabT and the concomitant up-regulation of 342 genes. Inhibition of the ERK1/2 pathway reduced but did not abolish the potentiation of the evoked response caused by GabT. In fact not all the genes analysed were blocked by ERK1/2 inhibitors.These results are in agreement with the notion that neuronal plasticity is mediated by several distinct pathways working in unison.We have used dissociated neuronal cultures grown over MEA for 2–6 weeks to monitor the electrical activity from a population of neurons [9]. MEAs allow stable and long lasting recordings (hours to days) of extracellular signals from the entire population and permit to characterize and follow the properties of single spikes from identified neurons. In this way, it was possible to describe the global properties of the network, such as its overall electrical activity and to obtain a characterization of changes during neuronal plasticity of single identified spikes. This analysis could not be performed with hippocampal slices or organotypic cultures grown on MEAs or in vivo, because in these cases local field potentials (LFPs) are observed and a detailed investigation of neuronal plasticity at a single spike level is almost impossible. We increased synaptic efficacy and the overall electrical activity by treating hippocampal cultures for 30 min. with the GABAA receptor antagonist gabazine (GabT). After GabT, gabazine was washed out and the time course of evoked electrical activity was followed/studied. MEA's extracellular electrodes were used for recording and stimulation so to quantify changes of the evok
Sor??o de atrazina e de mesotriona em latossolos e estimativa do potencial de contamina??o
Martinazzo, Rosane;Dick, Deborah Pinheiro;Hirsch, Mauro Mozael;Leite, Simone Benvenuti;Peralba, Maria do Carmo Ruaro;
Química Nova , 2011, DOI: 10.1590/S0100-40422011000800015
Abstract: following application herbicides usually reach the soil and undergo several dissipation processes which determine their effects on target organisms and on the environment. this work aimed to evaluate atrazine and mesotrione sorption in oxisols and estimate their potential impact on the environment. we observed that atrazine sorption was influenced by soil organic matter content and its hydrophobic character. clay fraction was the main factor influencing mesotrione sorption followed in a lesser extent by soil organic matter content. in contrast to atrazine, mesotrione retention was favored by the higher carboxylic substitution in the aliphatic chains of soil organic matter. atrazine and mesotrione were considered compounds that are likely to pose an appreciable risk of causing deleterious effects on the environment.
On the Dynamics of the Spontaneous Activity in Neuronal Networks
Alberto Mazzoni, Frédéric D. Broccard, Elizabeth Garcia-Perez, Paolo Bonifazi, Maria Elisabetta Ruaro, Vincent Torre
PLOS ONE , 2007, DOI: 10.1371/journal.pone.0000439
Abstract: Most neuronal networks, even in the absence of external stimuli, produce spontaneous bursts of spikes separated by periods of reduced activity. The origin and functional role of these neuronal events are still unclear. The present work shows that the spontaneous activity of two very different networks, intact leech ganglia and dissociated cultures of rat hippocampal neurons, share several features. Indeed, in both networks: i) the inter-spike intervals distribution of the spontaneous firing of single neurons is either regular or periodic or bursting, with the fraction of bursting neurons depending on the network activity; ii) bursts of spontaneous spikes have the same broad distributions of size and duration; iii) the degree of correlated activity increases with the bin width, and the power spectrum of the network firing rate has a 1/f behavior at low frequencies, indicating the existence of long-range temporal correlations; iv) the activity of excitatory synaptic pathways mediated by NMDA receptors is necessary for the onset of the long-range correlations and for the presence of large bursts; v) blockage of inhibitory synaptic pathways mediated by GABAA receptors causes instead an increase in the correlation among neurons and leads to a burst distribution composed only of very small and very large bursts. These results suggest that the spontaneous electrical activity in neuronal networks with different architectures and functions can have very similar properties and common dynamics.
Efeito bactericida do gerador de alta frequência na cultura de Staphylococcus aureus
Martins, Andiara;Silva, Josiceli Telles da;Graciola, Laureane;Fréz, Andersom Ricardo;Ruaro, Jo?o Afonso;Marquetti, Maria da Glória Karan;
Fisioterapia e Pesquisa , 2012, DOI: 10.1590/S1809-29502012000200011
Abstract: the purpose of this study was to check the bactericidal effect of the high frequency generator over the staphylococcus aureus culture. a total of 36 petri dishes inoculated with staphylococcus aureus were divided into 6 groups, including 4 treated (g5-15, g5-10, g3-15 and g3-10) and 2 controls (gc3 e gc5). g5-15 and g5-10 were treated 5 times per week during 15 and 10 minutes, respectively, while g3-15 and g3-10 were treated 3 times per week during 15 and 10 minutes, respectively. in treatment, it was used the high frequency generator with intensity of 10, sparking technique with standard electrode. after the 15th day of treatment, there were performed transplanting, in order to check if there were growth of new cultures, and only g5-15 showed to be effective when compared to gc5 (p=0.0039). so, the high frequency generator had a bactericidal effect on staphylococcus aureus in vitro culture at a frequency of 5 times per week and exposure time of 15 minutes daily.
Desenvolvimento de métodos de análise por CLAE-UV para os antimicrobianos tetraciclina, sulfametoxazol e trimetoprima utilizando materiais à base de sílica como sistemas de pré-concentra??o
Pedroso, Rosana Cristina Ribeiro;Peralba, Maria do Carmo Ruaro;Santos, Jo?o Henrique Zimnoch dos;Pizzolato, Tania Mara;Fr?ehlich, Pedro Eduardo;
Química Nova , 2011, DOI: 10.1590/S0100-40422011000200007
Abstract: this paper evaluates the adsorption capacity of zirconocene-based silica materials in the pre-concentration of antimicrobians (tetracycline, sulfamethoxazole and trimethoprim) in aqueous medium. these materials were prepared by grafting the zirconocene onto silicas pre-treated at different temperatures. the retention capacity of these materials was evaluated by off line spe and hplc-uv and the proposed methodology was validated in ultrapure, tap and river water. the recovery for tetracycline was 72% (in the solid phase a) and, for sulfamethoxazole and trimethoprim was 68 and 95% in the commercial c18, respectively. the target antimicrobians were not detected in the arroio dilúvio (porto alegre - rs).
Métodos de avalia??o da ferrugem do álamo e eficiência de fungicidas no seu controle
May De Mio, Louise Larissa;Ruaro, Lucimeris;
Revista árvore , 2008, DOI: 10.1590/S0100-67622008000500008
Abstract: poplar rust (melampsora medusae thuem.) causes serious damage in the plant nursery and its control is essential to obtain good quality seedlings. this work aimed at: i) testing the efficiency of contact fungicides (mancozebe, cartap and copper oxichloride) and systemic fungicides (triadimenol, tebuconazole and difenoconazole) for poplar rust control; ii) comparing evaluation methods to discriminate the efficiency among the treatments; iii) relating defoliation and disease data and iv) verify the influence of rust control under growth tree parameters. the experiment was conducted in s?o mateus do sul, pr and arranged in a completely randomized design with 10 treatments (check, triadimenol, mancozebe, tebuconazole, difenoconazole, cartap, copper oxichloride, triadimenol-mancozebe, tebuconazole-mancozebe and triadimenol applied at double the interval of the previous ones) and 11 repetitions. the experimental plots were 10 m wide with 4 planting rows (space between the lines was 2.5 and between the plants, 0.50 m), totaling 110 plots with the latorre clone. the following aspects were evaluated during two consecutive cycles: number of pustules per half leaf; visual percentage of the disease; severity per plot; visual percentage of defoliation, diameter at breast height and plant height at the end of the experiment. the systemic products (triadimenol, tebuconazole and difenoconazole) applied alone or interpolated with mancozebe were efficient in controlling the disease, reducing both the number of leaf pustules and defoliation, resulting in a significant gain in the final plant volume. mancozebe applied alone also reduced epidemics and increased tree volume in 42%. the cupric product made the volume increase in 27%. the evaluation methods used showed differences among the treatments and there was a correlation between the disease and the damage caused to the culture.
An overview and profile of the ICF's use in Brazil - a decade of history Panorama e perfil da utiliza o da CIF no Brasil : uma década de história
Jo?o A. Ruaro,Marinêz B. Ruaro,Dami?o E. Souza,Andersom R. Fréz
Brazilian Journal of Physical Therapy , 2012,
Abstract: BACKGROUND: In 2001the World Health Organization (WHO) adopted a classification system for understanding functioning and human disability: the International Classification of Functioning, Disability and Health (ICF). The ICF’s acceptance and use has been facilitated by the development and global consensus process, with increasing evidence about its validity. OBJECTIVES: To investigate the overview of the use of the ICF in Brazil, since its conception in 2001 until the year 2011. METHOD: We conducted an integrative literature review by searching SciELO, Lilacs, PubMed and ISI databases. To be included in the review, the study must have been published as scientific article, editorial or technical note, and had to having the participation of Brazilian researchers or have been developed in Brazil. RESULTS: One hundred and two publications were identified, but only 47 studies were included based in the inclusion criteria. Most of eligible studies were related to neurology (n=16) and orthopaedics (n=12) subdisciplines. The university that most appeared in the publications in national journals was The Universidade de S o Paulo (11) and in the international journals was Universidade Federal de Minas Gerais (4). In 2003 there was only one publication; in 2010 and 2011 were 10 and 8, respectively. CONCLUSIONS: Although incipient, the use of ICF in the Brazilian scientific community is rising. There is a concentration of studies related to the locomotor system, as well as performed by universities in the southeast area of Brazil. CONTEXTUALIZA O: Em 2001, a OMS aprovou um sistema de classifica o para o entendimento da funcionalidade e da incapacidade humana: a Classifica o Internacional de Funcionalidade, Incapacidade e Saúde (CIF). A aceita o e a utiliza o da CIF vêm sendo facilitadas pelo seu desenvolvimento e processo de consenso global, com crescente evidência sobre a sua validade. OBJETIVOS: Investigar o panorama do uso da CIF no Brasil desde a sua concep o em 2001 até o ano de 2011. MéTODO: Revis o integrativa da literatura com consulta às bases de dados SciELO, Lilacs, PubMed e ISI. Para ser incluído na revis o, o trabalho deveria ter sido publicado na forma de artigo científico, editorial ou nota técnica, além de ter a participa o de pesquisadores nacionais ou ter sido desenvolvido no Brasil. RESULTADOS: Foram identificadas 102 publica es; em seguida, a partir dos critérios de sele o, foram analisados 47 estudos, sendo a sua maioria nas áreas de neurologia (16) e ortopedia (12). A institui o de ensino que mais aparece nas publica es em periódico
An overview and profile of the ICF's use in Brazil - a decade of history Panorama e perfil da utiliza o da CIF no Brasil - uma década de história
Jo?o A. Ruaro,Marinêz B. Ruaro,Dami?o E. Souza,Andersom R. Fréz
Brazilian Journal of Physical Therapy , 2012, DOI: 10.1590/s1413-35552012005000063
Abstract: BACKGROUND: In 2001the World Health Organization (WHO) adopted a classification system for understanding functioning and human disability: the International Classification of Functioning, Disability and Health (ICF). The ICF’s acceptance and use has been facilitated by the development and global consensus process, with increasing evidence about its validity. OBJECTIVES: To investigate the overview of the use of the ICF in Brazil, since its conception in 2001 until the year 2011. METHOD: We conducted an integrative literature review by searching SciELO, Lilacs, PubMed and ISI databases. To be included in the review, the study must have been published as scientific article, editorial or technical note, and had to having the participation of Brazilian researchers or have been developed in Brazil. RESULTS: One hundred and two publications were identified, but only 47 studies were included based in the inclusion criteria. Most of eligible studies were related to neurology (n=16) and orthopaedics (n=12) subdisciplines. The university that most appeared in the publications in national journals was The Universidade de S o Paulo (11) and in the international journals was Universidade Federal de Minas Gerais (4). In 2003 there was only one publication; in 2010 and 2011 were 10 and 8, respectively. CONCLUSIONS: Although incipient, the use of ICF in the Brazilian scientific community is rising. There is a concentration of studies related to the locomotor system, as well as performed by universities in the southeast area of Brazil. CONTEXTUALIZA O: Em 2001, a OMS aprovou um sistema de classifica o para o entendimento da funcionalidade e da incapacidade humana: a Classifica o Internacional de Funcionalidade, Incapacidade e Saúde (CIF). A aceita o e a utiliza o da CIF vêm sendo facilitadas pelo seu desenvolvimento e processo de consenso global, com crescente evidência sobre a sua validade. OBJETIVOS: Investigar o panorama do uso da CIF no Brasil desde a sua concep o em 2001 até o ano de 2011. MéTODO: Revis o integrativa da literatura com consulta às bases de dados SciELO, Lilacs, PubMed e ISI. Para ser incluído na revis o, o trabalho deveria ter sido publicado na forma de artigo científico, editorial ou nota técnica, além de ter a participa o de pesquisadores nacionais ou ter sido desenvolvido no Brasil. RESULTADOS: Foram identificadas 102 publica es; em seguida, a partir dos critérios de sele o, foram analisados 47 estudos, sendo a sua maioria nas áreas de neurologia (16) e ortopedia (12). A institui o de ensino que mais aparece nas publica es em periódico
Cytogenetic studies in Phaseolus L. (Fabaceae)
Mercado-Ruaro, Pedro;Delgado-Salinas, Alfonso;
Genetics and Molecular Biology , 2000, DOI: 10.1590/S1415-47572000000400043
Abstract: a review of the cytogenetic studies carried out on phaseolus as well as the different proposals that have been suggested to explain the chromosomal changes in the group are presented. the importance of including wild species in cytogenetic studies and the collaboration between taxonomists and cytogeneticists in order to draw better conclusions are emphasized.
Diagnóstico da disposi??o final dos resíduos sólidos urbanos na regi?o da Associa??o dos Municípios do Alto Irani (AMAI), Santa Catarina
Fran?a,Rosiléa Garcia; Ruaro,édina Cristina Rodrigues;
Ciência & Saúde Coletiva , 2009, DOI: 10.1590/S1413-81232009000600026
Abstract: the aim of this study was to carry out diagnosis about the destination given to the urban solid wastes in amai-sc region, which has fourteen cities. it was applied questionnaires to the city halls, and visits to map active and inactive dumps, controlled and sanitary landfill. it was investigated: the existence of fences, monitoring and collecting of gas and leachate, if they were next to the superficial waters, the presence of animals or collectors. it was considered, also, the distance of houses and/or urban mass and information about selective collection. it was found twenty and seven inactive dumps, where eighteen had received treatment. it was detected precariousness in remediation of all inactive dumps, because a single covering with land is not enough, therefore the material put there, keeps polluting through the production of lard and methane gas. nowadays, all amai cities put their urban solid wastes in sanitary landfill found in xanxerê and xaxim. among the fourteen cities, only one has selective collection, and in a precarious way, what reduces the useful life time of landfill, beyond being harmful to the environment. it is collected 54.15 tons/day of wastes, which sums 0.610 g/hab.day of waste, costing r$ 1.78 to the public coffers monthly.
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