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Search Results: 1 - 10 of 71606 matches for " Maria Michela Mancarelli "
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A Novel, Non-canonical Splice Variant of the Ikaros Gene Is Aberrantly Expressed in B-cell Lymphoproliferative Disorders
Daria Capece, Francesca Zazzeroni, Maria Michela Mancarelli, Daniela Verzella, Mariafausta Fischietti, Ambra Di Tommaso, Rita Maccarone, Sara Plebani, Mauro Di Ianni, Alberto Gulino, Edoardo Alesse
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0068080
Abstract: The Ikaros gene encodes a Krüppel-like zinc-finger transcription factor involved in hematopoiesis regulation. Ikaros has been established as one of the most clinically relevant tumor suppressors in several hematological malignancies. In fact, expression of dominant negative Ikaros isoforms is associated with adult B-cell acute lymphoblastic leukemia, myelodysplastic syndrome, acute myeloid leukemia and adult and juvenile chronic myeloid leukemia. Here, we report the isolation of a novel, non-canonical Ikaros splice variant, called Ikaros 11 (Ik11). Ik11 is structurally related to known dominant negative Ikaros isoforms, due to the lack of a functional DNA-binding domain. Interestingly, Ik11 is the first Ikaros splice variant missing the transcriptional activation domain. Indeed, we demonstrated that Ik11 works as a dominant negative protein, being able to dimerize with Ikaros DNA-binding isoforms and inhibit their functions, at least in part by retaining them in the cytoplasm. Notably, we demonstrated that Ik11 is the first dominant negative Ikaros isoform to be aberrantly expressed in B-cell lymphoproliferative disorders, such as chronic lymphocytic leukemia. Aberrant expression of Ik11 interferes with both proliferation and apoptotic pathways, providing a mechanism for Ik11 involvement in tumor pathogenesis. Thus, Ik11 could represent a novel marker for B-cell lymphoproliferative disorders.
The tumor suppressor gene KCTD11REN is regulated by Sp1 and methylation and its expression is reduced in tumors
M Michela Mancarelli, Francesca Zazzeroni, Lucia Ciccocioppo, Daria Capece, Agnese Po, Simona Murgo, Raffaello Di Camillo, Christian Rinaldi, Elisabetta Ferretti, Alberto Gulino, Edoardo Alesse
Molecular Cancer , 2010, DOI: 10.1186/1476-4598-9-172
Abstract: TSGs often locate at chromosomal regions, which are frequently deleted and/or methylated in tumors. High levels of 17p13 somatic alterations have been showed in several tumors, distal and independent of the p53 locus [1-4].Our group has identified KCTD11 as an immediate-early gene induced by neurogenic signals [5] and encoding a novel adaptor of Cullin3 ubiquitin E3 ligase complex targeting Histone Deacetylase 1 [6]. Importantly, KCTD11 is a novel TSG that inhibits cell growth and is mapping on human chromosome 17p13.2, whose expression is frequently lost in human MB [4].To analyze whether the down-regulation of KCTD11 represents a specific feature of MB, as well to other cancers, we performed a wide screening for KCTD11 expression, analyzing 177 human tumor samples and 177 normal matching samples, representing 18 different cancer types. Normal tissues, including larynx, esophagus, stomach, colon-rectum, urinary bladder, lung, breast, gallbladder and endometrium, exhibited a nuclear KCTD11 positive immunohistochemical staining between 40 to 78% (Fig. 1B), whereas the matching tumor samples showed a significant reduction of 0 to 18% of nuclear KCTD11 staining (Fig. 1A and 1B). Reduced KCTD11 expression was not observed in thyroid and kidney tumor tissues vs normal suggesting a tumorigenic specific role of KCTD11 for the above mentioned tissues (Fig. 1A and 1B and data not shown). Moreover KCTD11 was undetected both in normal and cancer tissues from liver, lymph-node and exocrine pancreas (data not shown). Together, these findings clearly indicated that selective tissues expressing KCTD11 have down-regulated this gene during tumorigenesis.To understand the transcriptional regulation of KCTD11, we identified and analyzed the promoter region. Human KCTD11 proximal promoter is a 623 bp region (Fig. 2A). It turned out to be a TATA- and CAAT-less promoter. The transcription start site (TSS) was previously identified [4] (Fig. 2A). Using the TRANSFACT software, we identifie
The Use of Spatial Sampling Designs in Business Surveys  [PDF]
Maria Michela Dickson, Roberto Benedetti, Diego Giuliani, Giuseppe Espa
Open Journal of Statistics (OJS) , 2014, DOI: 10.4236/ojs.2014.45034

An innovative use of spatial sampling designs is here presented. Sampling methods which consider spatial locations of statistical units are already used in agricultural and environmental contexts, while they have never been exploited for establishment surveys. However, the rapidly increasing availability of geo- referenced information about business units makes that possible. In business studies, it may indeed be important to take into account the presence of spatial autocorrelation or spatial trends in the variables of interest, in order to have more precise and efficient estimates. The opportunity of using the most innovative spatial sampling designs in business surveys, in order to produce samples that are well spread in space, is here tested by means of Monte Carlo experiments. For all designs, the Horvitz-Thompson estimator of the population total is used both with equal and unequal inclusion probabilities. The efficiency of sampling designs is evaluated in terms of relative RMSE and efficiency gain compared with designs ignoring the spatial information. Furthermore, an evaluation of spatially balancing samples is also conducted.

Enhancing verbal episodic memory in older and young subjects after non-invasive brain stimulation
Rosa Manenti,Michela Brambilla,Michela Petesi,Clarissa Ferrari,Maria Cotelli
Frontiers in Aging Neuroscience , 2013, DOI: 10.3389/fnagi.2013.00049
Abstract: Memory is the capacity to store, maintain, and retrieve events or information from the mind. Difficulties in verbal episodic memory commonly occur in healthy aging. In this paper, we assess the hypothesis that anodal transcranial direct current stimulation (tDCS) applied over the dorsolateral prefrontal cortex (DLPFC) or over the parietal cortex (PARC) could facilitate verbal episodic memory in a group of 32 healthy older adults and in a group of 32 young subjects relative to a sham stimulation using a single-blind randomized controlled design. Each participant underwent two sessions of anodal tDCS (left and right) and one session of sham stimulation. Overall, our results demonstrated that, in young and in older subjects, anodal tDCS applied during the retrieval phase facilitates verbal episodic memory. In particular, we found that tDCS applied over the left and right regions (DLPFC and PARC) induced better performance in young participants; only tDCS applied over the left regions (DLPFC and PARC) increased retrieval in older subjects. These results suggest that anodal tDCS can be a relevant tool to modulate the long-term episodic memory capacities of young and older subjects.
Anodal tDCS during face-name associations memory training in Alzheimer's patients
Maria Cotelli,Rosa Manenti,Michela Brambilla,Michela Petesi,Sandra Rosini
Frontiers in Aging Neuroscience , 2014, DOI: 10.3389/fnagi.2014.00038
Abstract: Objective: Given the limited effectiveness of pharmacological treatments, non-pharmacological interventions to treat Alzheimer's disease (AD) have gained attention in recent years. The aim of the present study is to investigate the effects of anodal tDCS (AtDCS) combined with memory training on face-name associations in an AD patient sample.
Sources of Variability in the Use of Standardized Perfusion Value for HCC Studies  [PDF]
Michela D’Antò, Mario Cesarelli, Francesco Fiore, Maria Romano, Paolo Bifulco
Open Journal of Medical Imaging (OJMI) , 2012, DOI: 10.4236/ojmi.2012.22006
Abstract: Hepatocellular carcinoma (HCC) is one of the world’s most common malignant tumours. As known, liver tumour tissue is characterised by an increased blood supply related to neoangionesis which causes an increased arterial vascularisation. CT Perfusion Imaging is an important, non invasive, technique for qualitative assessment of tissue perfusion after contrast agent administration. Nevertheless, being able to reliably quantifying angiogenesis is increasingly important to both the evaluation of the disease progression and monitoring of the therapeutic response of HCC. With this in mind, we believe that could be helpful to employ Standardised Perfusion Value (SPV), which has the potential to be a useful non-invasive marker of HCC angiogenesis. However, before using SPV in clinical practice, we need to verify its reliability. There are different causes of variability in applying the SPV index, e.g., the technical specifications of the CT system employed and the image processing system. In this paper the authors will analyse the variability of the BFa estimates and the variability due to the calibration procedure of the CT system, this with the objective of verifying how these factors affects SPV values. In our case, perfusion MDCT images of seventeen HCC patients were analysed. A software application, based on maximum slope method, was developed to compute BFa and SPV values. Four radiologists were involved in images processing evaluating variability related to ROI selection; each radiologist repeated the ROI drawing four times on the same image set. We computed the k calibration factor in order to evaluate SPV variability due to calibration protocol of CT systems. Results show that calibration factor variance, due to the position in the gantry, is less than BFa variability. So, we conclude that, when daily calibration is preferred, a simplified protocol, which neglects the dependence of K factor from the position, may be utilised; at least until the intrinsic variability of perfusion parameter computation operator-dependent will be reduced.
Local Excision of Early Rectal Cancer by Transanal Endoscopic Microsurgery (TEM): The 23-Year Experience of a Single Centre  [PDF]
Mario Guerrieri, Monica Ortenzi, Maria Michela Cappelletti Trombettoni, Indrit Kubolli, Roberto Ghiselli
Journal of Cancer Therapy (JCT) , 2015, DOI: 10.4236/jct.2015.611108
Abstract: Aim: Transanal endoscopic microsurgery (TEM) is an effective, minimally invasive alternative approach to traditional surgery. This study reviews the characteristics of a series of patients affected by early rectal cancer and discusses the results of this treatment. Methods: From 1992 to 2014, 187 patients with rectal cancer staged as pT1 by preoperative endorectal ultrasound, computerized tomography and/or magnetic resonance imaging were treated by TEM at our institution. We analysed age, gender, size of lesion, distance from the anal verge, histological grading and stage. Furthermore we considered operative time, intra and post-operative complications and hospital stay. Patients were also enrolled in a tight follow-up for recurrence and survival. Results: There were no intraoperative complications or conversions to other procedures. There were minor complications (partial suture dehiscence, stool incontinence, rectal haemorrhage) in 24 patients (12.8%) and a major complication (perianal phlegmon) in one (1.5%). Two (5%) of the 40 patients with pT3 disease before neoadjuvant therapy experienced a local recurrence and one (2.5%) died for metastasis. Conclusion: TEM is a safe technique characterized by low morbidity and mortality and excellent oncological outcomes. These advantages, coupled with its ability to be applied to a strikingly high proportion of rectal tumours, suggest that it should be considered as the gold standard approach to early rectal cancer in accurately selected patients.
How Can the Microbiologist Help in Diagnosing Neonatal Sepsis?
Michela Paolucci,Maria Paola Landini,Vittorio Sambri
International Journal of Pediatrics , 2012, DOI: 10.1155/2012/120139
Abstract: Neonatal sepsis can be classified into two subtypes depending upon whether the onset of symptoms is before 72 hours of life (early-onset neonatal sepsis—EONS) or later (late-onset neonatal sepsis—LONS). These definitions have contributed greatly to diagnosis and treatment by identifying which microorganisms are likely to be responsible for sepsis during these periods and the expected outcomes of infection. This paper focuses on the tools that microbiologist can offer to diagnose and eventually prevent neonatal sepsis. Here, we discuss the advantages and limitation of the blood culture, the actual gold standard for sepsis diagnosis. In addition, we examine the utility of molecular techniques in the diagnosis and management of neonatal sepsis.
Tobacco attributable morbidity and hospital costs in Piedmont: forecast for the years 2003-2014
Elisabetta Versino,Maria Michela Gianino,Giovanni Renga
Italian Journal of Public Health , 2007, DOI: 10.2427/5892
Abstract: Background: Tobacco smoke is the main cause of mortality and morbidity in most industrialized countries. The aim of this study is to forecast the smoke-related morbidity for the residents in Piedmont for the years 2003-2014 and the relative costs for the regional health service, using as an indicator the number of hospital admissions caused by smoke and as an instrument the DRG rates. Methods: The model uses the risk of hospitalisation among non smokers to predict smoke related morbidity for the period 2003-2014 for both smokers and ex-smokers, by using relative risks (RRs) and smoking prevalence. It should be noted that, because of the 15-year latency between smoke exposure and health outcomes, smoking prevalence of the appropriate time period has been applied to the morbidity data of the following 15 years, thus because of the shift of birth cohorts we are able to make forecasts up until the year 2014. Basing on these data it is possible estimate, separately for smokers and ex-smokers, the aetiological fraction (PAR%) used to estimate smoking attributable admissions and smoking attributable costs. The costs attributable to admissions for smoke-related diseases have been estimated using prices set for 2002 as well as prices adjusted for inflation. Results: A total of 145801 hospitalizations are expected among men and 36959 among females for the period 2003-2014. The economic value of the attributable admissions, at prices adjusted for inflation, increases in the period 2003-2014 with a slowdown in 2014. Data show that in 2014, compared to 2003, a smaller amount of resources, in true value, have been allocated to smoking related admissions (- 11.08%). Conclusions. The model used meta-analytic RR real prevalence data, considering a fifteen-year latency period between exposure and its effect on health. Furthermore, an economic estimate is made for each DRG instead of applying medium rates for Major Diagnostic Categories as is frequently seen at a national level.
Tobacco smoke in Piedmont: attributable morbidity and impact on hospital costs
Elisabetta Versino,Maria Michela Gianino,Giovanni Renga
Italian Journal of Public Health , 2006, DOI: 10.2427/5939
Abstract: Background: Tobacco smoke is the main cause of mortality and morbidity in most industrialized countries. The aim of this research is to estimate the smoke attributable morbidity for Piedmont residents in the years 1997-2002 and the related costs for the regional health service, using as an indicator the number of hospital admissions caused by smoke and as an instrument the DRG rates. Methods: extraction of hospital admissions for smoking-related diseases; estimate of the proportion of hospital admittances attributable to smoking; estimate of the overall cost and the smoke attributable cost for each year. Results: The attributable proportion in men is clearly higher than in women. In men it decreases from 10.6% in 1997 to 8.2% in 2002, while values among women seem to have reached a plateau. The economic value of the attributable admissions, at current prices, shows a downward trend for men from 1997 to 2001 and an upward one in the year 2002, while for women the trend is fluctuating. Conclusions: This method demonstrates the following original features: we used metanalytic relative risks real prevalence data, considering a fifteen-year latency period between exposure and effect on health.The model that we developed adheres more adherent to the natural history of the disease and to the local health problem, giving us a useful tool for planning purposes.Furthermore, the economic estimate is made for each single DRG instead of applying medium rates for MDC, which happens more frequently at the national level.
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