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Search Results: 1 - 10 of 388493 matches for " Maria Marluce S Vilela "
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Avalia??o da inflama??o com base na libera??o de radicais oxidantes por granulócitos na asma cr?nica n?o-controlada
Sartorelli, Cristina F.;Rehder, Jussara;Condino Neto, Antonio;Vilela, Maria Marluce S.;
Jornal de Pediatria , 2009, DOI: 10.1590/S0021-75572009000200010
Abstract: objective: to evaluate spontaneous release of superoxide anion by peripheral blood granulocytes of atopic patients with uncontrolled asthma undergoing glucocorticoid therapy and of healthy subjects. methods: we studied 32 patients, aged 6 to 18 (mean 12.04), and 29 healthy subjects as a comparative group. patients were grouped according to the forced expiratory vital capacity in the first second. group i, forced expiratory vital capacity in the first second of between 60 and 80%, had 19 patients, and group ii, forced expiratory vital capacity in the first second = 60%, had 13 patients. spontaneous superoxide release by granulocytes was measured by a spectrophotometer method based on superoxide dismutase, before and after oral prednisone and beclomethasone, budesonide or fluticasone inhaled therapy. statistical analyses were performed using anova, wilcoxon and tukey tests. results: comparing the superoxide anion release by granulocytes of asthmatic patients and healthy subjects, we observed a higher release by cells of the uncontrolled patient group ii (p < 0.05). evaluating the superoxide release by cells of asthmatic patients before and after steroid therapy, a significant decrease was found only in patient group i. conclusion: the impact of corticosteroids on inflammatory modulation occurred in the uncontrolled asthmatics with forced expiratory vital capacity in the first second between 60 and 80%. in those with forced expiratory vital capacity in the first second of = 60%, this finding was not observed. further studies are necessary to evaluate the effect of this finding on asthmatic patients.
Aspectos clínicos e o hemograma em crian?as expostas ao HIV-1: compara??o entre pacientes infectados e soro-reversores
Silva, Elianete B.;Grotto, Helena Z.W.;Vilela, Maria Marluce S.;
Jornal de Pediatria , 2001, DOI: 10.1590/S0021-75572001000600014
Abstract: objective: to analyze the evolution of clinical and hematological aspects of children exposed to the vertical transmission of hiv-1, comparing infected patients with uninfected ones or seroreverters. methods: prospective, descriptive, longitudinal study. we analyzed 79 children born from hiv-1 infected mothers, under clinical follow up from march, 1996 until november, 1997, at the immunodeficiency division of the hospital de clínicas da unicamp (state university hospital of campinas). results: failure to thrive was observed in both groups, but was greater among seroreverters. among the infected children, 23 mothers did not use azt during pregnancy, 16 of them (61.5%) had been breastfed, four were classified into clinical category n, seven into a and fifteen into b. clinical manifestations in patients younger than one year were seen in 18 infected children (69.2%). anemia was observed in 73.1% of the infected group and in 41.5% of the seroreverters (p < 0.008). the comparison between the groups showed that the most common hematologic alterations in the infected children was microcytosis and hypochromia (p < 0.05), lymphopenia between 15 and 18 months (p < 0.05), monocytosis between 9 and 12 months (p < 0.05) and a tendency towards high ferritin levels, with no statistical significance. conclusions: microcytic and hypochromic anemia were observed in both groups: iron deficiency in the uninfected children, and chronic disease anemia in the infected ones. the infected children presented with monocytosis and lymphopenia at an earlier stage.
Estratégias de campo em ensaios clínicos com novas vacinas produzidas no Brasil Estrategias de campo en ensayos clínicos con nuevas vacunas producidas en Brasil Clinical trials field strategies with novel vaccines produced in Brazil
Emília de Faria Carniel,Maria ?ngela R. G. M. Ant?nio,Maria de Lurdes Zanolli,Maria Marluce S. Vilela
Revista Paulista de Pediatria , 2012, DOI: 10.1590/s0103-05822012000200008
Abstract: OBJETIVO: Relatar as estratégias de campo utilizadas em dois ensaios clínicos com vacinas desenvolvidas pelo Instituto Butantan, em 2004 e 2006. MéTODOS: Estudo do tipo relato de experiência, em que se descreve o planejamento e a operacionaliza o dos ensaios clínicos, que avaliaram a imunogenicidade e a seguran a da vacina BCG combinada com a vacina da hepatite B (VrHB-IB) e da tetravalente bacteriana modificada pela extra o do lipopolissacarídeo (LPS) do componente pertussis (DTPm/Hib). RESULTADOS: As principais estratégias de campo utilizadas foram: a) Parceria entre os pesquisadores e os gestores da Secretaria Municipal de Saúde e b) Realiza o dos procedimentos da pesquisa nos domicílios ou nos Centros de Saúde frequentados pelos participantes. No primeiro estudo, foram vacinados 552 recém-nascidos na maternidade com a BCG/VrHB-IB (combinadas ou separadas) e nos domicílios, com as duas doses subsequentes de VrHB-IB. O segundo estudo incluiu 241 lactentes em Centros de Saúde da rede municipal, vacinados com tetravalente bacteriana (com componente pertussis total ou modificado). Em ambos os estudos, amostras de sangue foram colhidas nas residências. N o houve relatos de eventos adversos. A ades o foi de 90,2% para o primeiro estudo e 93,8%, para o segundo. As vacinas foram administradas nas datas preconizadas pelo Programa Nacional de Imuniza es e as coletas de sangue, de acordo com o cronograma dos estudos. CONCLUS ES: As estratégias utilizadas facilitaram o recrutamento das crian as e garantiram cumprir o protocolo da pesquisa com alta ades o, sem interferir no vínculo da família com o Servi o de Saúde, no calendário vacinal ou no seguimento pediátrico dos participantes. OBJETIVO: Relatar las estrategias de campo utilizadas en dos ensayos clínicos con vacunas desarrolladas por el Instituto Butantan, en 2004 y 2006. MéTODOS: Estudio de tipo relato de experiencia, en que se describe la planificación y la operacionalización de los ensayos clínicos, que evaluaron la imunogenicidad y la seguridad de la vacuna BCG combinada con la vacuna de la hepatitis B (VrHB-IB) y de la tetravalente bacteriana modificada por la extracción del lipopolisacárido (LPS) y del componente pertussis (DPTm/Hib). RESULTADOS: Las principales estrategias de campo utilizadas fueron: a) Colaboración entre los investigadores y los gestores de la Secretaría Municipal de Salud y b) Realización de los procedimientos de la investigación en los domicilios o Centros de Salud frecuentados por los participantes. En el primero estudio, se vacunaron 552 recién nacidos en la maternidad con la
The impact of maternal HIV infection on cord blood lymphocyte subsets and cytokine profile in exposed non-infected newborns
Eliane Borges-Almeida, Helaine MBPM Milanez, Maria Marluce S Vilela, Fernanda GP Cunha, Beatriz M Abramczuk, Suiellen C Reis-Alves, Konradin Metze, Irene Lorand-Metze
BMC Infectious Diseases , 2011, DOI: 10.1186/1471-2334-11-38
Abstract: In a prospective, controlled study, 36 mother-child pairs from HIV+ mothers and 15 HIV-uninfected mothers were studied. Hematological features and cytokine profiles of mothers at 35 weeks of pregnancy were examined. Maternal and cord lymphocyte subsets as well as B-cell maturation in cord blood were analyzed by flow cytometry. The non-stimulated, as well as BCG- and PHA-stimulated production of IL2, IL4, IL7, IL10, IL12, IFN-γ and TNF-alpha in mononuclear cell cultures from mothers and infants were quantified using ELISA.After one year follow-up none of the exposed infants became seropositive for HIV. An increase in B lymphocytes, especially the CD19/CD5+ ones, was observed in cord blood of HIV-exposed newborns. Children of HIV+ hard drug using mothers had also an increase of immature B-cells. Cord blood mononuclear cells of HIV-exposed newborns produced less IL-4 and IL-7 and more IL-10 and IFN-γ in culture than those of uninfected mothers. Cytokine values in supernatants were similar in infants and their mothers except for IFN-γ and TNF-alpha that were higher in HIV+ mothers, especially in drug abusing ones. Cord blood CD19/CD5+ lymphocytes showed a positive correlation with cord IL-7 and IL-10. A higher maternal age and smoking was associated with a decrease of cord blood CD4+ cells.in uninfected infants born to HIV+ women, several immunological abnormalities were found, related to the residual maternal immune changes induced by the HIV infection and those associated with antiretroviral treatment. Maternal smoking was associated to changes in cord CD3/CD4 lymphocytes and maternal hard drug abuse was associated with more pronounced changes in the cord B cell line.HIV infection is associated with a complex pattern of changes in the hemopoietic and the immune systems, resulting in abnormalities of peripheral blood (PB) counts and changes in T and B lymphocytes. Decrease of T helper and increase of cytotoxic lymphocytes, profound changes in the cytokine profile and a
Imunoglobulinas séricas em crian?as com exposi??o perinatal ao vírus da imunodeficiência humana
Silva, Marcos T. N. da;Centeville, Maraisa;Tani, Sergio M.;Toro, Adyleia A. D. C.;Rossi, Claudio;Vilela, Maria Marluce dos S.;
Jornal de Pediatria , 2001, DOI: 10.1590/S0021-75572001000300012
Abstract: objective: hypergammaglobulinemia is an early manifestation of perinatal hiv infection. our objective was to analyze the differences in serum immunoglobulin levels between infected and seroreverting children and their association with clinical outcome. methods: we carried out a historical prospective study with 107 infected and 90 seroreverting children. we compared the iga, igg, and igm levels between infected and seroreverting patients within the first 18 months of life; iga, igg, and igm as surrogate markers of infection; and iga, igg, and igm levels within the first 5 years in infected children, according to clinical outcome. the mann-whitney test was used for comparison between groups. surrogate markers were assessed according to sensitivity, specificity, positive and negative predictive values, and youden's index. results: infected children, when compared to seroreverters, showed significantly higher levels of igm between the 1st and 5th trimesters; iga and igg between the 2nd and 6th trimesters (p less than or equal to 0.05). levels of iga greater than or equal to 90 mg/dl in the 2nd trimester and igg greater than or equal to 1,700 mg/dl or 1,200 mg/dl in the 2nd and 3rd trimesters were associated with hiv infection, with youden's indexes of 0.97, 0.92, and 0.93, respectively. infected children in the b and c categories, compared to those in the n and a, showed higher levels of igm between the 2nd and 4th years, and iga between the 3rd and 5th year (p > 0.05). conclusions: the temporal progression of iga, igg, and igm levels showed an early and intense stimulation to the synthesis of immunoglobulin in infected children. clinical and epidemiological indicators showed that such levels may be surrogate markers of infection. higher igm and iga levels between the 2nd and 5th years in more severely infected children suggest a dysfunction in immune regulation secondary to persistent antigenic stimulation.
The Importance of Myeloperoxidase in Apocynin-Mediated NADPH Oxidase Inhibition
Ana Carolina de Almeida,Maria Marluce dos Santos Vilela,Antonio Condino-Neto,Valdecir F. Ximenes
ISRN Inflammation , 2012, DOI: 10.5402/2012/260453
Abstract:
The Importance of Myeloperoxidase in Apocynin-Mediated NADPH Oxidase Inhibition
Ana Carolina de Almeida,Maria Marluce dos Santos Vilela,Antonio Condino-Neto,Valdecir F. Ximenes
ISRN Inflammation , 2012, DOI: 10.5402/2012/260453
Abstract: Apocynin is widely used as an inhibitor of the NADPH oxidase. Since myeloperoxidase (MPO) has been considered as essential for the mechanism of action of apocynin, here we used cells with different levels of MPO and compared their sensitivity to apocynin. HL-60 cells were differentiated with DMSO or IFNγ/TNFα and compared with peripheral mononuclear (PBMC) and polymorphonuclear cells (PMN). The relative MPO activity was PBMC = HL60 DMSO < HL60 IFNγ < PMN. Apocynin inhibited the intracellular reactive oxygen species production by PMN (80%) and IFNγ/TNFα-differentiated HL-60 cells (45%) but showed a minor effect in PBMC and DMSO differentiated HL-60 cells (20%). The addition of azide decreased the efficiency of apocynin in PMN and the addition of peroxidase increased the inhibition in PBMC. We also determined the gene expression of the components gp91phox, p47phox, p22phox and p67phox in the resting cells. Apocynin did not change gp91phox, p47phox or p22phox gene expression in nonstimulated PBMC, HL60 DMSO, HL60 IFNγ/TNFα, and PMN and has a subtle increase in p67phox in HL60 IFNγ/TNFα. The results from this work suggest that a rational search for better inhibitors of NADPH oxidase in leukocytes should include a correlation with their affinity as substrates for MPO. 1. Introduction NADPH oxidases (NOXs) comprise a family of multicomponent enzymatic systems that catalyze the reduction of molecular oxygen to superoxide anion radical [1–3]. The best characterized and studied member of the NOX family is NOX2, which is expressed in phagocytic cells and has its primordial microbicidal function in the innate immune system as the primary source of reactive oxygen species (ROS). NOX2 is comprised by cytosolic factors p47phox, p67phox, and p40phox and the membrane-linked p22phox and gp91phox factors, which are the catalytic subunit. NOX2 also has a regulatory GTPase, Rac2 (in neutrophils), or Rac1 (in monocytes) [4, 5]. NOXs, including NOX2, are also expressed in endothelial cells and have direct influence in the oxidative imbalance, which is considered a pivotal event in the endothelial dysfunction and, consequently, in the initiation and/or progression of chronic inflammatory and vascular diseases [5]. Hence, the inhibition or modulation of NOXs activities may have direct benefit in the treatment of these pathologies [5]. Apocynin (4-hydroxy-3-methoxyacetophenone) is widely used as an inhibitor of NOXs and of the concomitant ROS production in experimental models involving phagocytic [6–8] and nonphagocytic cells [9–13]. The effects of apocynin include the
Research on zinc blood levels and nutritional status in adolescents with autoimmune hepatitis
Pereira, Thalita Cremonesi;Saron, Margareth L. G.;Carvalho, Wagner Alves de;Vilela, Maria Marluce;Hoehr, Nelci Fenalti;Hessel, Gabriel;
Arquivos de Gastroenterologia , 2011, DOI: 10.1590/S0004-28032011000100013
Abstract: context: zinc deficiency in children and adolescents impairs their growing, development and immune system. objective: to verify the existence of plasma and leukocyte zinc deficiency in adolescents with autoimmune hepatitis. methods: the study comprised 23 patients with autoimmune hepatitis, aged 10-18 years, assisted at the ambulatory service of pediatric hepatology of the university of campinas teaching hospital, campinas, sp, brazil, and adolescents with ages compatible with the patients' ages comprised the control group. sample of blood in both groups was collected for the analyses of plasma zinc and leukocyte zinc by atomic absorption spectrophotometry, beyond the nutritional status was evaluated in each adolescent. the following statistical tests were used: mann-whitney, spearman's correlation and interclass concordance analysis. results:the significance level adopted was 5%. the average zinc level in plasma in patients was 71.91 ± 11.79 μg/dl and, in the control group, it was 80.74 ± 10.92 μg/dl, showing a significant difference (p = 0.04). the leukocyte zinc level in patients was 222.33 ± 166.13 pmol/106 cells and, in the control group, it was 226.64 ± 217.81 pmol/106 cells; there was no statistical significance between them (p = 0.45). conclusion:the evaluation of the nutritional status showed that eutrophy is prevalent in patients, and they presented a higher body fat value than the control group, with a significant difference. more research is needed with adolescents with autoimmune hepatitis regarding levels of essential micronutrients, such as zinc, because a good nutritional status can improve the prognostic of liver disease.
Implementa??o oportuna de interven??es para reduzir a transmiss?o vertical do HIV: uma experiência brasileira bem-sucedida
Amaral,Eliana; Assis-Gomes,Francisco; Milanez,Helaine; Cecatti,José Guilherme; Vilela,Maria Marluce; Silva,Jo?o Luiz Pinto e;
Revista Panamericana de Salud Pública , 2007, DOI: 10.1590/S1020-49892007000500003
Abstract: objective: to describe the impact, at the public maternity facility of a university hospital in brazil, of the rapid implementation of new guidelines recommended by national consensus panels concerning the prevention of vertical hiv transmission. method: we performed a retrospective study of deliveries by hiv-infected women at the public maternity facility of a university hospital in the city of campinas, s?o paulo, brazil, from 1990 through 2000. the guidelines utilized at the facility during this period were: (1) from 1990 through 1994, contraindication to breast-feeding and no use of antiretroviral drugs; (2) 1995 and 1996, use of zidovudine (azt) by the pregnant woman and the newborn; (3) 1997 and 1998, use of azt according to the actg 076 protocol; and (4) 1999 and 2000, multiple antiretroviral agents and elective cesarean delivery. all the antiretroviral drugs were provided for free by brazil's public health care system. the vertical transmission rate was calculated for each of the four stages, and the risk ratio for congenital transmission was calculated for each stage and for each prophylactic intervention separately (breast-feeding, type of antiretroviral drug, type of delivery). results: we studied 197 deliveries at the public maternity facility over that 1990-2000 period. over the four stages, the rate of vertical transmission decreased: it was 32.3% in the first stage, 25.7% in the second, 2.2% in the third, and 2.9% in the fourth. the most pronounced decrease, observed from the second to the third stage, occurred after introduction of the full actg 076 regimen. the use of combined antiretroviral agents increased from 0% in the first stage to 46.4% in the fourth stage. there were no cases of vertical transmission in pregnant women treated with multiple drugs. the risk of vertical hiv transmission was 5 times as great with breast-feeding vs. no breast-feeding (risk ratio = 5.06), 5 times as great with no antiretroviral therapy vs. the full actg 076 regime
Acellular and "low" pertussis vaccines: adverse events and the role of mutations
Higashi, Hisako G.;Luna, Expedito;Precioso, Alexander R.;Vilela, Marluce;Kubrusly, Flávia S.;Dias, Waldely O.;Raw, Isaias;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2009, DOI: 10.1590/S0036-46652009000300002
Abstract: objective: to discuss the current paho recommendation that does not support the substitution of traditional cellular dtp vaccine by acellular dtp, and the role of mutations, in humans, as the main cause of rare adverse events, such as epileptic-like convulsions, triggered by pertussis vaccine. data review: the main components related to toxic effects of cellular pertussis vaccines are the lipopolysaccharide of bacterial cell wall and pertussis toxin. the removal of part of lipopolysaccharide layer has allowed the creation of a safer cellular pertussis vaccine, with costs comparable to the traditional cellular vaccine, and which may be a substitute for the acellular vaccine. conclusion: the new methodology introduced by instituto butantan allows for the development of a new safer pertussis vaccine with low lps content (plow), and the use of the lipopolysaccharide obtained in the process in the production of monophosphoryl lipid a. this component has shown potent adjuvant effect when administered together with influenza inactivated vaccine, making possible to reduce the antigen dose, enhancing the production capacity and lowering costs.
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