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Search Results: 1 - 10 of 230378 matches for " Maria Letícia de Castro;Fumian "
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Therapeutic approaches for tumor necrosis factor inhibition
Barbosa, Maria Letícia de Castro;Fumian, Milla Machado;Miranda, Ana Luísa Palhares de;Barreiro, Eliezer J.;Lima, Lídia Moreira;
Brazilian Journal of Pharmaceutical Sciences , 2011, DOI: 10.1590/S1984-82502011000300002
Abstract: tumor necrosis factor (tnf) consists of an inflammatory cytokine essential for homeostasis and organism defense. despite its physiological relevance, both increased biosynthesis and release of tnf lead to the exacerbation of inflammatory and oxidative responses, which are related to the pathogenesis of a host of diseases of an inflammatory, autoimmune and/or infectious nature. in this context, effective therapeutic approaches for the modulation of tnf have been the focus of research efforts. approximately one million individuals worldwide have been treated with biotechnological inhibitors of this cytokine, the so-called anti-tnf biopharmaceuticals. however, given the high risk of infection and the limitations related to cost and administration routes, new therapeutic approaches aimed at biological targets that directly or indirectly modulate the production and/or activation of tnf appear promising alternatives for the discovery of new anti-inflammatory and immunomodulatory orally active drugs and are therefore discussed in this paper.
Gaspar Malone,Paulo Dejalma Zimmer,Maria Alice da Silva de Castro,Letícia Noemi Arias
Pesquisa Agropecuária Tropical , 2007, DOI: 10.5216/pat.v37i2.1823
Abstract: Red rice is one of the most important weeds of cultivated rice in Brazil. On the other hand, red rice constitutes a collection of genes lost during the cultivated rice domestication process and has a fundamental importance for recovery of promising genic constitutions. This study had the objective to analyze the genetic variability of a colletion of red rice (Oryza sativa L.) ecotypes from Rio Grande do Sul State, Brazil, through isoenzimatic and microsatellite markers. Thirty four accesses from the Seed and Biotechnology Laboratory germplasm bank, at Federal University of Pelotas, were analyzed using six isoenzimatic systems and nineteen microsatellite markers. Twenty-three biochemical and 54 molecular alleles were identified and used to estimate the polymorphism (PIC) and genetic similarity indexes. The analyzed red rice population presents large genetic variability, evidencing the potentiality to map characteristics of interest to seed physiology. Biochemical markers of the isoenzime type and molecular markers of the microsatellite type are efficient to estimate the genetic variability in red rice ecotypes. KEY-WORDS: Oryza sativa; biochemical markers; microsatellite markers; genetic variability. O arroz vermelho é uma das principais espécies daninhas para cultivo de arroz no Brasil. Por outro lado, constitui-se num acervo de genes perdidos durante a domestica o do arroz cultivado, que poderá ser importante para a recupera o de constitui es gênicas promissoras. O objetivo desde trabalho foi analisar a variabilidade genética de uma cole o de acessos de arroz vermelho (Oryza sativa L.) coletados no Estado de Rio Grande do Sul, Brasil, através de marcadores bioquímicos do tipo isoenzimas, e marcadores moleculares do tipo microssatélites. Trinta e quatro acessos, pertencentes ao banco de germoplasma do Laboratório de Sementes e Biotecnologia, da Universidade Federal de Pelotas, foram analisados utilizando seis sistemas isoenzimáticos e dezenove marcadores microssatélites. Vinte e três alelos bioquímicos e 54 moleculares foram identificados e utilizados para estimar os índices de polimorfismo (PIC) e de similaridade genética. Os resultados indicam que a popula o de arroz vermelho analisada apresenta grande variabilidade genética, evidenciando potencialidade para o mapeamento de características de interesse à fisiologia de sementes. Marcadores bioquímicos do tipo isoenzimas e marcadores moleculares do tipo microssatélite s o eficientes para estimar a variabilidade genética em acessos de arroz vermelho. PALAVRAS-CHAVE: Oryza sativa, marcadores bioquími
Isolation of human umbilical cord blood-derived osteoprogenitor cells: a promising candidate for cell-based therapy for bone repair
Igor Iuco Castro-Silva,Letícia de Oliveira Castro,Janaína José dos Santos Machado,Maria Helena Alves Nicola
Einstein (S?o Paulo) , 2011,
Abstract: Objective: The aim of this study was to evaluate the osteogenic potential of human umbilical cord blood-derived osteoprogenitor cells and to prove its applicability as a promising candidate for cell-based therapeutics for bone repair. Methods: Primary cultures of human umbilical blood cord adherent cells were expanded in vitro until passage 2 and seeded for osteodifferentiation study. Morphological (light microscopy), cytochemical (Von Kossa’s method), and functional analyses (calcium level, alkaline phosphatase activity, and total protein content in cell culture) were carried out 7, 14, 21, and 28 days after the osteoinduction protocol. Results: The proliferative step showed colony-forming units in 7 days. After osteoinduction, cuboidal cellular morphology similar to osteoblasts at 14 days and mineralization nodules and biochemical changes (increased alkaline phosphatase level and calcium deposits) at 21 days confirmed the osteodifferentiation process. Conclusion: Cell culture of human umbilical blood cord is a reliable technique, constituting itself as an alternative source of osteoprogenitor cells for experimental needs. More animal tests and clinical trials must be carried out to validate its use and to establish quality control of future autologous or allogeneic cell-based therapy aimed at bone repair.
Ingestive behavior of steers grazing under different heights of Tanzania grass Comportamento ingestivo de novilhos de corte sob diferentes alturas de pastejo do capim Tanzania
Rondineli Pavezzi Barbero,Marco Aurélio Alves de Freitas Barbosa,Letícia Maria de Castro,Edson Luis de Azambuja Ribeiro
Semina : Ciências Agrárias , 2012,
Abstract: Ingestive behavior of steers grazing in four heights of Tanzania grass was evaluated. 12 paddocks of one hectare each, with three replicates for each height, and three males Nelore cattle, per paddock, more regulators at the time, were used. Time and time daytime grazing, ruminating and resting, rate of eating and rumination, length of grazing cycles and rumination, were observed. Forage samples were collected at ground level and grazing simulation to assess possible selectivity. The experimental design was completely randomized, and analysis of variance was performed for continuous variables to regression procedures, and discrete variables in tests of means (P < 0.05). The highest heights had high bit rates, while the smallest was higher expenditure of time in search for forage mass. Grazing time decreased linearly as increased the canopy height, however the opposite was observed for rumination. At all canopy heights was observed selectivity of forage for the animals. Foi avaliado o comportamento ingestivo de bovinos em quatro alturas de pastejo do capim Tanzania. Foram utilizados 12 piquetes de um hectare cada, com três repeti es para cada altura, utilizando três bovinos nelore, machos por piquete, mais reguladores de altura. Foram observados horários e tempo diurno de pastejo, rumina o e ócio, taxa de bocados e rumina es, dura o dos ciclos de pastejo e rumina o, além de serem colhidas amostras de forragem ao nível do solo e por simula o de pastejo para avaliar possível seletividade de forragem pelos animais. O delineamento foi inteiramente casualizado, e foi realizada análise de variancia, sendo que nas variáveis contínuas aplicaram-se a regress o, e nas variáveis discretas, testes de médias (P < 0,05). A taxa de bocados apresentou comportamento linear crescente conforme aumento da altura do dossel, enquanto que nas menores houve maior dispêndio de tempo na busca por massa de forragem. O tempo de pastejo foi linear decrescente conforme aumento da altura do dossel, mas o contrário foi observado para rumina o. Em todas as alturas foi observada seletividade de forragem pelos animais.
Micropropaga??o e conserva??o de Macrosyphonia velame (St. Hil.) Muell. Arg. em banco de germoplasma in vitro
Martins, Letícia Maria;Pereira, Ana Maria Soares;Fran?a, Suzelei de Castro;Bertoni, Bianca Waléria;
Ciência Rural , 2011, DOI: 10.1590/S0103-84782011005000015
Abstract: root extracts of macrosyphonia velame an apocynaceae native of brazilian cerrado, known as white velame have been popularly used as depurative and anti-syphilitic agent. the aim of the present research was to develop a micropropagation protocol for the in vitro conservation of m. velame in a germplasm bank. seeds of velame collected in sacramento, tapira and araxá, mg, brazil, were used as initial explants. nodal segments from axenic plantlets were inoculated on ms/2 medium supplemented with different concentrations (0.25, 5.0 and 1.0mg l-1) of bap; kinetin; 2ip or tdz. for in vitro rooting , plantlets (2cm high) were inoculated on ms/2 medium supplemented with iba or naa (1.0, 5.0, and 10.0mg l-1), maintained for 5, 10 and 30 days and sub-cultured to ms/2 medium for an additional thirty days before evaluating rooting. for acclimatization and ex vitro rooting plantlets were transplanted into styrofoam boxes containing either plantmax?, sand and soil one by one or in combinations (1:1) of sand/plantmax?; sand/soil; plantmax?/soil. for the in vitro conservation of m. velame in germplasm bank plantlets (3.5cm high) were inoculated on ms/2 medium supplemented with either 2% sucrose + 4% of mannitol or sorbitol; 2% sucrose + 4% mannitol or sorbitol + 2mg l-1 calcium pantothenate; 2% of sucrose + 4% of mannitol or sorbitol + 2mg l-1 spermidine. the proportion of seed germination was considered low, 33%, 4% and 2% for seeds collected in araxá, tapira and sacramento respectively. explants cultured on ms/2 medium without addition of cytokinin showed enhanced height (5.2cm), increased number of buds (8.6), proliferation of 4 shoots per bud and minimal (4%) proportion of vitrification. plantlets acclimatized ex vitro developed better in plantmax? substrate, most plantlets presented root formation and survival reached 40%. m. velame plantlets cultured for three months on ms/2 added with 2% sucrose + 4% mannitol + 2mg l-1 of calcium pantothenate, under germoplasma bank condition
Economic feasibility of four grazing intensities of Tanzania grass for beef cattle Viabilidade econ mica de quatro intensidades de pastejo do capim Tanzania na pecuária de corte
Rondineli Pavezzi Barbero,Marco Aurélio Alves de Freitas Barbosa,Letícia Maria de Castro,Edson Luís de Azambuja Ribeiro
Semina : Ciências Agrárias , 2012,
Abstract: Were evaluated the economic effects of four grazing heights (20, 40, 60 and 80 cm) of Tanzania grass for beef cattle. The total area was 12 acres divided into paddocks of one hectare, with three replications. We used three animals, males, Nelore per paddock, as more animals need additional adjustment to the height you want. The reduction in sward height allowed higher stocking rate, which, even with a reduction in individual performance, there was more beef production per area. The interest rate on capital given the property represented the largest share in the final cost of production. The participation of fixed variables showed a positive linear function of the increase in height from grazing, showing significant reduction of the production scale. A reduction in cost of production per hectare per year with increasing grazing height. No differences were found in certain economic indicators, and the four systems remunerate the capital invested. Foram avaliados os efeitos econ micos de quatro alturas de pastejo (20; 40; 60 e 80 cm) do capim Tanzania para bovinos de corte. A área total foi de 12 hectares divididos em piquetes de um hectare, com três repeti es. Foram utilizados três bovinos, machos, Nelore por piquete, mais animais adicionais conforme necessidade de ajuste para as alturas pretendidas. A redu o da altura do pasto permitiu maior taxa de lota o, onde, mesmo com redu o no desempenho individual, houve maior produ o por área. A taxa de juros atribuída sobre o capital do imóvel representou a maior parcela no custo final de produ o. A participa o das variáveis fixas apresentou comportamento linear positivo em fun o do aumento na altura dos pastos, se mostrando sensível à redu o da escala de produ o. Houve redu o do custo de produ o anual por hectare com aumento da altura de pastejo. N o foram detectadas diferen as nos indicadores econ micos determinados, e os quatro sistemas remuneraram o capital investido.
Synthesis and Pharmacological Evaluation of Novel Phenyl Sulfonamide Derivatives Designed as Modulators of Pulmonary Inflammatory Response
Maria Letícia de Castro Barbosa,Thiago José Figueira Ramos,Ana Carolina Santos de Arantes,Marco Aurélio Martins,Patrícia Machado Rodrigues e Silva,Eliezer J. Barreiro,Lídia Moreira Lima
Molecules , 2012, DOI: 10.3390/molecules171214651
Abstract: In this paper we report the design, synthesis and pharmacological evaluation of a new series of phenyl sulfonamide derivatives 2a–h and 3–8 planned by structural modification on the anti-inflammatory prototype LASSBio-468 (1). Among the synthesized analogues, the tetrafluorophthalimide LASSBio-1439 (2e) stands out showing an in vitro anti-TNF-α effect similar to the standard thalidomide. The relevance of tetrafluorination of the phthalimide nucleus was also confirmed by the anti-inflammatory profile of 2e, through oral administration, in a murine model of pulmonary inflammation. The corresponding tetrafluorocarboxyamide metabolite LASSBio-1454 (15), generated from partial hydrolysis of the derivative 2e, presented a significant in vitro effect and a pronounced anti-inflammatory activity in vivo.
Fatores de risco para o prolapso genital em uma popula??o brasileira
Rodrigues, Andrea Moura;Oliveira, Letícia Maria de;Martins, Karina de Falco;Roy, Carlos Ant?nio Del;Sartori, Marair Gracio Ferreira;Gir?o, Manoel Jo?o Batista Castello;Castro, Rodrigo de Aquino;
Revista Brasileira de Ginecologia e Obstetrícia , 2009, DOI: 10.1590/S0100-72032009000100004
Abstract: purpose: to evaluate risk factors for the development of genital prolapse in the brazilian population. methods: case-control study involving 316 patients submitted to prolapse staging, according to the pelvic organ prolapse quantification system. the patients were divided into two groups: in the case group there were 107 patients with prolapse at stage iii or iv, and in the control group, 209 women at stage 0 or i. in the anamnesis, the selected women have been questioned about the presence of possible risk factors for genital prolapse, such as: age, menopause age, parturition, delivery type (vaginal, caesarean section or forceps), occurrence of fetal macrosomia, family history of genital dystopia in first degree relatives, chronic cough and intestinal constipation. results: the variables that were different between the groups were: age, body mass index, parturition, number of vaginal, caesarean section or forceps deliveries, newborn weight and positive family history for prolapse. race, menopause age, chronic cough and intestinal constipation did not present differences between the groups. after logistic regression, only three variables have been shown to be independent risk factors: presence of at least one vaginal delivery, fetal macrosomia and positive family history for dystopia. cesarean section was shown to be a protective factor. conclusion: in the brazilian population, the independent risk factor for genital prolapse were: personal antecedent of at least one vaginal delivery, fetal macrosomia and family history of dystopia.
Tendência temporal da amamenta??o na cidade do Rio de Janeiro: 1996-2006
Castro,Inês Rugani Ribeiro de; Engstrom,Elyne Montenegro; Cardoso,Letícia Oliveira; Dami?o,Jorginete de Jesus; Rito,Rosane Valéria Fonseca Viana; Gomes,Maria Auxiliadora de Souza Mendes;
Revista de Saúde Pública , 2009, DOI: 10.1590/S0034-89102009005000079
Abstract: objective: to analyze time trend in breast-feeding (bf) and exclusive breast-feeding (ebf). methods: data from a monitoring system, based on surveys conducted during the national immunization campaign in the city of rio de janeiro, southeastern brazil, in 1996, 1998, 2000, 2003 and 2006, were analyzed. study population was comprised of 19,044 children younger than one year of age, who were present in vaccination stations. a probability cluster sample (vaccination stations), self-weighted and representative of the population of children younger than 12 months of age (<12), was studied for each year. a structured questionnaire with closed questions about the child's diet at the moment of the study and maternal sociodemographic characteristics was applied. the bf and ebf indicators proposed by the world health organization were adopted. results: bf<12 increased from 61.3% to 73.4% between 1996 and 2006. similar trend was observed in all age groups analyzed. ebf in children <4 and <6 months of age (ebf<6) increased from 18.8% to 42.4% and from 13.8% to 33.3%, respectively. improvements in bf>6 and ebf<6 were found in all categories of all maternal sociodemographic variables. for ebf<6, the disadvantage observed in women with a lower level of education in 1996 and in women who worked in 1998 was not completely overcome by 2006. conclusions: bf and ebf increased in the period studied, independently from child age group and maternal socio-demographic characteristics. the differences found among women in distinct sociodemographic situations were not completely overcome.
Letícia Benedetti F. de Castro
SaBios : Revista de Saúde e Biologia , 2006,
Abstract: The populational density of Cedrela fissilis Vell. was evaluated. In a secondary forest area in Parque Estadual Lago Azul from Campo Mour o/PR, twenty-four parcels of 20m x 25m were used, totalizing 12,000 m2. Measures of circumference at breast height (CBH) were taken, as well as distinctions between young, adult and old individuals were established. 74 individuals have been sampled: 38 young, 20 adult and 16 old individuals. It was observed that density of Cedrela fissilis population was 61,6 individuals in each hectare represented by: 51,35% of young, 27.02% of adult and 21,61% of old individuals. Results, in accordance with searched literature, are significant and indicate that the secondary forest of the Parque Estadual Lago Azul is passing by a reconstitution phase.
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