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Search Results: 1 - 10 of 283133 matches for " Maria Lúcia Guerra Monteiro "
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Quality of Semi-Prepared Products from Rainbow Trout Waste (Onchorynchus mykiss) by Using Different Technological Strategics  [PDF]
Karoline Ribeiro Palmeira, Eliane Teixeira Mársico, Laís Doro, M?sar Lemos, Claudia Emília Teixeira, Vania Margaret Flosi Paschoalin, Maria Lúcia Guerra Monteiro, Carlos Adam Conte Júnior
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.56067
Abstract:

The consumption of freshwater fish and fish products has gradually grown worldwide over the last decades, generating a proportional waste increase. The objective of the present study was to assess the chemical and bacteriological quality of restructured fish product, meatball-type, prepared with rainbow trout (Onchorynchus mykiss) waste added of 1% transglutaminase (MTG), 4% textured soy protein (TSP) and replacing part of the sodium chloride with potassium chloride (75%/25%) as described below: T1—starch addition (control); T2—MTG addition (1%); T3—soy protein addition (4%); T4—soy protein addition (4%) and MTG addition (1%); T5—soy protein addition (4%), MTG addition (1%) and partial replacement of salt (75% NaCl/25% KCl). Total aerobic mesophilic bacteria (TAMB), 2-thiobarbituric acid reactive substances (TBARS), pH determination and quantification of biogenic amines were performed on the day after manufacturing (P0) and after 60 days of storage (P1) at -25℃ ± 2℃. The results showed that there was no significant difference (p < 0.05) of microbiological quality, TBARS and pH after storage. T4 presented the lowest total biogenic amine content (256.84 mg/kg) whereas T3 and T5 had the highest value (791.36 and 707.19 mg/kg, respectively) in this parameter. Putrescine was the biogenic amine that presented the highest concentration (504.00 mg/kg) in T3 and cadaverine that presented the smallest concentration (0.36 mg/kg) in T4. The use of technological strategies for developing new products with non-commercial fillets kept the most standards, having changes only in some biogenic amines.

Validade comercial de filés de Tilápia do Nilo (Oreochromis niloticus) resfriados embalados em atmosfera modificada e irradiados
Monteiro, Maria Lúcia Guerra;Mársico, Eliane Teixeira;Teixeira, Claudia Emília;Mano, Sérgio Borges;Conte Júnior, Carlos Adam;Vital, Helio de Carvalho;
Ciência Rural , 2012, DOI: 10.1590/S0103-84782012000400027
Abstract: this paper investigated the effects of modified atmosphere packing (60% n2 and 40% co2) and irradiation (with a 1.5kgy dose) either used alone or combined on the shelf life of fillets of refrigerated nile tilapia. a total of 120 fillets were used in the experiments in order to test four different treatments and included samples: packed in air (control), packed in modified atmosphere, packed in air and irradiated, and finally, packed in modified atmosphere and irradiated. regular monitoring of physicochemical parameters (tvb, ph, ammonia and tbars), bacteriological conditions (heterotrophic aerobic mesophilic and psychrotrophic bacteria) and sensory acceptance were performed. the results consistently indicated an increasing deterioration of the fillets with time, however those treated with irradiation, modified atmosphere or both combined had longer shelf lives (around two weeks) in comparison with the control (4 days only).
Trypanosoma cruzi IV Causing Outbreaks of Acute Chagas Disease and Infections by Different Haplotypes in the Western Brazilian Amazonia
Wuelton Marcelo Monteiro, Laylah Kelre Costa Magalh?es, Amanda Regina Nichi de Sá, M?nica Lúcia Gomes, Max Jean de Ornelas Toledo, Lara Borges, Isa Pires, Jorge Augusto de Oliveira Guerra, Henrique Silveira, Maria das Gra?as Vale Barbosa
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041284
Abstract: Background Chagas disease is an emergent tropical disease in the Brazilian Amazon Region, with an increasing number of cases in recent decades. In this region, the sylvatic cycle of Trypanosoma cruzi transmission, which constitutes a reservoir of parasites that might be associated with specific molecular, epidemiological and clinical traits, has been little explored. The objective of this work is to genetically characterize stocks of T. cruzi from human cases, triatomines and reservoir mammals in the State of Amazonas, in the Western Brazilian Amazon. Methodology/Principal Findings We analyzed 96 T. cruzi samples from four municipalities in distant locations of the State of Amazonas. Molecular characterization of isolated parasites from cultures in LIT medium or directly from vectors or whole human blood was performed by PCR of the non-transcribed spacer of the mini-exon and of the 24 S alfa ribosomal RNA gene, RFLP and sequencing of the mitochondrial cytochrome c oxidase subunit II (COII) gene, and by sequencing of the glucose-phosphate isomerase gene. The T. cruzi parasites from two outbreaks of acute disease were all typed as TcIV. One of the outbreaks was triggered by several haplotypes of the same DTU. TcIV also occurred in isolated cases and in Rhodnius robustus. Incongruence between mitochondrial and nuclear phylogenies is likely to be indicative of historical genetic exchange events resulting in mitochondrial introgression between TcIII and TcIV DTUs from Western Brazilian Amazon. TcI predominated among triatomines and was the unique DTU infecting marsupials. Conclusion/Significance DTU TcIV, rarely associated with human Chagas disease in other areas of the Amazon basin, is the major strain responsible for the human infections in the Western Brazilian Amazon, occurring in outbreaks as single or mixed infections by different haplotypes.
Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice
Wuelton Marcelo Monteiro ,Ana Paula Margioto Teston,Ana Paula Gruendling,Daniele dos Reis,M?nica Lúcia Gomes,Silvana Marques de Araújo,Maria Terezinha Bahia,Laylah Kelre Costa Magalh?es,Jorge Augusto de Oliveira Guerra,Henrique Silveira,Max Jean de Ornelas Toledo,Maria das Gra?as Vale Barbosa
PLOS Neglected Tropical Diseases , 2013, DOI: 10.1371/journal.pntd.0002069
Abstract: Background In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test the hypothesis that T. cruzi clonal structure has a major impact on its biological and medical properties. Methodology/Principal Findings Seventeen parameters were assayed in mice infected with 14 T. cruzi strains belonging to DTU TcI and 11 strains typed as TcIV. In comparison with TcI, TcIV stocks promoted a significantly shorter pre-patent period (p<0.001), a longer patent period (p<0.001), higher values of mean daily parasitemia (p = 0.009) and maximum of parasitemia (p = 0.015), earlier days of maximum parasitemia (p<0.001) and mortality (p = 0.018), higher mortality rates in the acute phase (p = 0.047), higher infectivity rates (p = 0.002), higher positivity in the fresh blood examination (p<0.001), higher positivity in the ELISA at the early chronic phase (p = 0.022), and a higher positivity in the ELISA at the late chronic phase (p = 0.003). On the other hand TcI showed higher values of mortality rates in the early chronic phase (p = 0.014), higher frequency of mice with inflammatory process in any organ (p = 0.005), higher frequency of mice with tissue parasitism in any organ (p = 0.027) and a higher susceptibility to benznidazole (p = 0.002) than TcIV. Survival analysis showing the time elapsed from the day of inoculation to the beginning of the patent period was significantly shorter for TcIV strains and the death episodes triggered following the infection with TcI occurred significantly later in relation to TcIV. The notable exceptions come from positivity in the hemocultures and PCR, for which the results were similar. Conclusion/Significance T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
When the Silence Speaks: The Smile  [PDF]
Maria do Rosário Dias, Letícia Garcia Naben, Ana Lúcia Monteiro, Ana Ferreira, Valter Pedroso Alves, Ana Delgado
Journal of Biosciences and Medicines (JBM) , 2018, DOI: 10.4236/jbm.2018.611002
Abstract: Background: Understanding the importance, value and self-image that the smile represents in the daily life of the individual remains a subject of high scientific interest. Aim: The present qualitative and exploratory study aims at understanding the importance of the self-perception of the mouth and smile on the mental representation of self-image and the well-being of the individual. Materials and Methods: The convenience sample consisted of 151 children and youngsters of both genders, ages 8 - 24 years, who used an orthodontic appliance and were invited to draw two portraits of their mouth/smile. Results: The results suggest differences in the mental representation of the mouth and the smile, namely in the investment of the drawings before and after the use of the orthodontic appliance, as well as in the expressiveness and manifestation of emotions. Discussion and Conclusions: The results suggest that the main reason that leads participants to the treatment is related to aesthetic and functional issues in which the maximizing of the mental representation of the Orthodontic Smile emerges as a new categorization of the perfect smile.
Critical electrolyte concentration of chromatin in polytene chromosomes of Trichosia pubescens (Diptera, Sciaridae)
Monteiro, Ana Lúcia P.;Mello, Maria Luiza S.;
Genetics and Molecular Biology , 1998, DOI: 10.1590/S1415-47571998000200003
Abstract: the binding of toluidine blue molecules to nucleic acid phosphates under conditions of competition with mg2+ ions was studied in trichosia pubescens polytene chromosome regions differing in composition and organization (rna-rich areas and puffs, dna puffs, heterochromatin). the aim was to find variability in the mg2+ concentration at which metachromasy was completely prevented (= critical electrolyte concentration; cec) and that could reflect differences at the level of nucleoprotein complexes in particular chromosome regions and developmental phases. although high cec values have been found in tightly packed chromatin such as that of heterochromatin zones, the cec values for the other regions have proven to be affected not only by availability and proximity of dna phosphates but also by rna richness and other factors. among these, changes in dna geometry and packing state have been assumed for explaining increased values after rna removal in dna puffs and rna-rich zones. based on cec values it has been suggested that alterations at the level of nucleoprotein complexes may occur in puffs before they are morphologically detectable. cec results for polytene chromosomes were thus revealed to vary considerably with slight variations in nucleoprotein composition and organization. however, since a complex and apparently contradictory rationale has to be used for explaining part of the results, cec is not recommended as a useful tool for extensive and comparative studies of this particular model.
Critical electrolyte concentration of chromatin in polytene chromosomes of Trichosia pubescens (Diptera, Sciaridae)
Monteiro Ana Lúcia P.,Mello Maria Luiza S.
Genetics and Molecular Biology , 1998,
Abstract: The binding of toluidine blue molecules to nucleic acid phosphates under conditions of competition with Mg2+ ions was studied in Trichosia pubescens polytene chromosome regions differing in composition and organization (RNA-rich areas and puffs, DNA puffs, heterochromatin). The aim was to find variability in the Mg2+ concentration at which metachromasy was completely prevented (= critical electrolyte concentration; CEC) and that could reflect differences at the level of nucleoprotein complexes in particular chromosome regions and developmental phases. Although high CEC values have been found in tightly packed chromatin such as that of heterochromatin zones, the CEC values for the other regions have proven to be affected not only by availability and proximity of DNA phosphates but also by RNA richness and other factors. Among these, changes in DNA geometry and packing state have been assumed for explaining increased values after RNA removal in DNA puffs and RNA-rich zones. Based on CEC values it has been suggested that alterations at the level of nucleoprotein complexes may occur in puffs before they are morphologically detectable. CEC results for polytene chromosomes were thus revealed to vary considerably with slight variations in nucleoprotein composition and organization. However, since a complex and apparently contradictory rationale has to be used for explaining part of the results, CEC is not recommended as a useful tool for extensive and comparative studies of this particular model.
Effect of an Educational Intervention by Attendance and at Distance on Nurses’ Knowledge about Pressure Ulcer  [PDF]
Elaine M. L. R. Andrade, Patrícia A. L. Cavalcanti, Ana Karoline C. Monteiro, Ana Karine C. Monteiro, Maria Helena B. A. Luz, José M. M. Neto, Isabel A. C. Mendes
Creative Education (CE) , 2014, DOI: 10.4236/ce.2014.518185
Abstract: The effect of an educational intervention by attendance and at distance on the knowledge of nurses about pressure ulcer (PU) was verified. An experimental research with a post-test control group was undertaken. A randomized sample of 43 nurses in a Control Group (CG) (N = 20) was submitted to an educational intervention by attendance and an Experimental Group (EG) (N = 23) was submitted to an intervention at distance. Data were collected between January and April 2012 in three phases, which were: location, invitation, registration and adaptation of the nurses to Moodle, educative intervention and post-test. The mean number of correct answers on the posttest among the nurses who participated in the intervention by attendance was 34.0 (sd = 3.3), against 36.2 (sd = 2.7) for the nurses in the distance education intervention. This difference in means was statistically significant (p = 0.019). The research evidenced that continuing education in nursing through distance education (DE) can be effective due to its practicality and interactivity and because it allows nurses in clinical practice to decide on the best time and place to access the content and improve their knowledge about PU.
Perfil nutricional de pacientes candidatos ao transplante de pulm?o
Souza, Sabrina Monteiro Pereira de;Nakasato, Miyoko;Bruno, Maria Lúcia Mendes;Macedo, Alessandra;
Jornal Brasileiro de Pneumologia , 2009, DOI: 10.1590/S1806-37132009000300008
Abstract: objective: to determine the nutritional profile of lung transplant candidates. methods: a retrospective cross-sectional study involving lung transplant candidates at a public hospital in the city of s?o paulo, brazil. data related to gender, age and underlying lung disease were compiled for the participants. for the assessment of the nutritional profile of the patients, body mass index (bmi), mid-arm muscle circumference (mamc), waist circumference and triceps skinfold thickness (tst) were determined during the first outpatient visit. results: we included 117 patients, 69 of which (59%) were male. the mean age of the participants was 42.5 ± 15.2 years. the most prevalent underlying disease, seen in 29 patients (24.8%), was pulmonary emphysema. the nutritional profile was considered normal in 48.3% of the patients with pulmonary emphysema, 55% of those with cystic fibrosis, 56% of those with bronchiectasis and 50% of those with other lung diseases. the majority (51.7%) of the patients with pulmonary fibrosis were classified as overweight. the tsts indicated that the risk of depletion was highest (64.7%) among the patients with cystic fibrosis, followed by those with bronchiectasis (52.6%). conclusions: patients with pulmonary fibrosis presented the highest bmis, although the corresponding tsts and mamcs were normal. patients with cystic fibrosis and bronchiectasis presented the highest prevalence of nutritional depletion, based on tst and mamc.
A qualidade de vida de jovens portadores de espinha bífida do Children's National Medical Center ? Washington DC
Soares,Ana Helena Rotta; Moreira,Martha Cristina Nunes; Monteiro,Lúcia Maria Costa; Pohl,Hans G.;
Ciência & Saúde Coletiva , 2006, DOI: 10.1590/S1413-81232006000300029
Abstract: the present article explores the quality of life of adolescents with spina bifida at the children's national medical center in washington dc. the research is based on a qualitative perspective that utilizes the "theory of stigma" as the central theoretical framework, in addition to the discussions of the concept of quality of life and its dimensions. fifteen adolescents were interviewed at the above institution, eight of which then participated in a focal group structured through issues that emerged during this previous phase. the discourses reflected the need for more encompassing categories for quality of life that do not pulverize the life experience. the adolescent's discourses demonstrate an inequality structure in interactions between the healthy and the disabled. the results demonstrated that stigma permeates all life dimensions of these subjects, interfering in their social inclusion, as well as in their subjective construction and self-esteem, asserting the role of the disabled as a "non-person". finaly, we found that even though sexuality was not prioritized in other instruments that contemplate this population, the theme emerges as central to the discussion of their life experiences.
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