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Search Results: 1 - 10 of 401201 matches for " Maria Esther de N;Buzar "
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Genotipagem de polimorfismos associados com sistemas de macho-esterilidade em acessos de cebola adaptados ao Brasil
Ragassi, Carlos Francisco;Santos, Maria do Desterro M dos;Fonseca, Maria Esther de N;Oliveira, Valter R;Buzar, Anne Giselle R;Costa, Cyro P da;Boiteux, Leonardo S;
Horticultura Brasileira , 2012, DOI: 10.1590/S0102-05362012000300009
Abstract: the synthesis of onion (allium cepa) hybrids relies upon the use of two genetic-cytoplasmic male-sterility systems, cms-s and cms-t, in association to the normal male-fertile (n) cytoplasm. however, the molecular phenotyping of male-sterility-inducing and normal cytoplasms of many onion accessions adapted for cultivation under tropical conditions is not available. some of these accessions were reported as presenting tolerance to diseases and adaptation to tropical and subtropical regions. therefore, these accessions are potential sources of parental lines for the brazilian onion hybrid breeding programs. in the present work, polymerase chain reaction (pcr) assays were conducted using mitochondria-derived markers in order to characterize the cytoplasm type present in different morpho-agronomic onion types cultivated in brazil. the s cytoplasm appeared to be the most frequent one among the 66 evaluated accessions with a frequency of 56%. the t male-sterility-inducing cytoplasm was observed in 25.8% of the accessions. of the samples, 18.2% displayed exclusively the cytoplasm n. this information could be useful to guide the onion breeding programs in the selection of parental lines aiming to develop hybrid cultivars adapted to tropical and subtropical areas of the world.
Diversidade genética em germoplasma tropical de cebola estimada via marcadores RAPD
Santos, Maria do Desterro M dos;Ragassi, Carlos Francisco;Fonseca, Maria Esther de N;Buzar, Anne Gizelle R;Oliveira, Valter R;Melo, Paulo César T de;Boiteux, Leonardo S;
Horticultura Brasileira , 2012, DOI: 10.1590/S0102-05362012000100019
Abstract: onion is a crop of significant socioeconomic importance to brazil. onion germplasm with adaptation to tropical and sub-tropical conditions has played an important role in the development of this crop in the country. in this context, we studied the genetic diversity in a germplasm collection potentially useful for the development of cultivars for tropical and subtropical regions. the genetic variability of 21 accessions/cultivars that have been used as germplasm and/or were developed by onion breeding programs in brazil was evaluated via rapd markers. the following accessions were included in the study :'red creole', 'roxa ipa-3', 'valenciana 14', 'beta cristal', 'diamante', 'composto ipa-6', 'aurora', 'bojuda rio grande', 'alfa tropical', 'pêra ipa-4', 'primavera', 'belém ipa-9', 'crioula alto vale', 'conquista', 'pira-ouro', 'vale-ouro ipa-11', 'franciscana ipa-10', 'serrana', 'cnph 6400', 'petroline', and 'baia periforme'. from the 520 primers used in the initial screening only 38 displayed stable polymorphisms. they produced 624 amplicons, of which 522 (83.7%) were monomorphic and 102 (16.3%) were polymorphic. an average similarity coefficient of 0.72 was calculated among accessions based upon this subgroup of polymorphic amplicons. this allowed the discrimination of this germplasm collection into six groups with only one of them comprising more than one accession. the main group was formed by 16 accessions ('diamante', 'composto ipa-6', 'aurora', 'bojuda rio grande', 'conquista', 'pira-ouro', 'serrana', 'vale-ouro ipa-11', 'baia periforme', 'primavera', 'franciscana ipa-10', 'belém ipa-9', 'crioula alto vale', 'petroline', 'pêra ipa-4' and 'alfa tropical'), for which the genetic origin (with few exceptions) can be traced back to the variety 'baia periforme'. the populations 'red creole', 'roxa ipa-3', 'beta cristal', 'cnph 6400', and 'valenciana 14' comprised a set of five isolated groups, showing genetic divergence among them and in relation to main 'baia perif
Nucleotide diversity of a major carotenoid biosynthetic pathway gene in wild and cultivated Solanum (Section Lycopersicon) species
Araújo, Ana H.;Fonseca, Maria Esther de N.;Boiteux, Leonardo S.;
Brazilian Journal of Plant Physiology , 2007, DOI: 10.1590/S1677-04202007000300007
Abstract: a polymerase chain reaction (pcr)-based approach was employed to isolate putative alleles of the chromoplast-specific lycopene beta-cyclase (cycb) gene from wild and cultivated tomatoes [solanum (section lycopersicon)]. the objective of this work was to establish an effective pcr protocol by testing dna samples from distinct germplasm accessions with a primer pair designed to selectively target conserved regions present in the available cycb sequences. this pcr optimization allowed the amplification of 1219 out 1666 bp of the gene in six taxa: s. cheesmaniae, s. peruvianum, s. neorickii, s. pennellii, s. pimpinellifolium and s. lycopersicum. sixty-three mutation sites (31 transitions, 18 transversions and 14 single base deletions/insertions) were detected in these accessions when compared to s. lycopersicum af 254793 (used as reference sequence). the polymorphisms were found predominantly in green-fruited species (20 in s. neorickii, 20 in s. peruvianum, and 32 in s. pennellii). lower levels of polymorphisms were found in yellow-fruited (three in s. cheesmaniae) and red-fruited species (eight in s. pimpinellifolium and none in the s. lycopersicum). the higher levels of nucleotide diversity in the cycb-like gene sequences in accessions of green-fruited species as well as the phylogenetic tree agreed with the previous taxonomic studies based upon the granule-bound starch synthase gene phylogeny. sequence analyses of the amplicons obtained via heterologous pcr indicated the cycb gene-specificity of the primers. therefore, this pcr-based strategy might be useful to isolate cycb-like amplicons from other species within the genus solanum and to develop molecular markers for assisted breeding.
Actividad antimicrobiana de Senna alata L
Guerra Ordo?ez,Marta; Sánchez Govín,Esther; Gálvez Blanco,Maria de los Angeles;
Revista Cubana de Plantas Medicinales , 2004,
Abstract: medicinal plants are an important natural source in the search for new compounds with pharmacological activities of interest. senna alata (caesalpinaceae) grows in cuba and has a wide popular use.the in vitro antimicrobial activity of different formulations prepared from this species was evaluated. the method of dilutions in liquid medium was used and the minimum inhibitory and/or minimum concentration against microorganisms of human clinical interest were determined. the antimicrobial potential of s. alata, specially the antimicrobial activity of the fluid extract with menstruum 70 % was proved. the kynetics of the antimicrobial activity corroborated the results obtained that justified the traditional use of this medicinal plant and, at the same time, allows the preparation of ointments with a marked dermatophytic activity.
Actividad antimicrobiana de Senna alata L
Marta Guerra Ordo?ez,Esther Sánchez Govín,Maria de los Angeles Gálvez Blanco
Revista Cubana de Plantas Medicinales , 2004,
Abstract: Las plantas medicinales constituyen una fuente natural importante en la búsqueda de nuevos compuestos con actividades farmacológicas de interés. Senna alata (Caesalpinaceae) crece en Cuba y tiene un amplio uso popular. En el presente trabajo fue evaluada la actividad antimicrobiana in vitro de diferentes formulaciones elaboradas a partir de esta especie. Se empleó el método de diluciones en medio líquido y se determinó la concentración mínima inhibitoria y/o mínima microbicida frente a microorganismos de interés clínico humano. Fue demostrado el potencial antimicrobiano de S. alata, especialmente la actividad antimicrobiana del extracto fluido con menstruo al 70 %. La cinética de la actividad antimicrobiana corroboró los resultados obtenidos, los que justifican el uso tradicional de esta planta medicinal y a la vez permite elaborar cremas con una notable actividad antidermatofítica. Medicinal plants are an important natural source in the search for new compounds with pharmacological activities of interest. Senna alata (Caesalpinaceae) grows in Cuba and has a wide popular use.The in vitro antimicrobial activity of different formulations prepared from this species was evaluated. The method of dilutions in liquid medium was used and the minimum inhibitory and/or minimum concentration against microorganisms of human clinical interest were determined. The antimicrobial potential of S. alata, specially the antimicrobial activity of the fluid extract with menstruum 70 % was proved. The kynetics of the antimicrobial activity corroborated the results obtained that justified the traditional use of this medicinal plant and, at the same time, allows the preparation of ointments with a marked dermatophytic activity.
Hortali?as como alimentos funcionais
Carvalho, Patrícia G B de;Machado, Cristina Maria M;Moretti, Celso Luiz;Fonseca, Maria Esther de N;
Horticultura Brasileira , 2006, DOI: 10.1590/S0102-05362006000400001
Abstract: although a very old concept, the relationship between food and health has never been as close as it is today. diets rich in fat, salt, and sugar and poor in complex carbohydrates, vitamins, and minerals in association with a more sedentary lifestyle, are responsible for an increase in diet-related diseases such as obesity, diabetes, cardiovascular problems, hypertension, osteoporosis, and cancer. it is believed that the ingestion of fruits and vegetables helps in the prevention of these diseases. vegetables are an important component of the diet, usually in association with protein- and starch-rich foods. they are responsible not only for adding variety of color and texture to meals, but also for providing important nutrients. vegetables are low fat and low calorie foods, with relatively small amounts of protein, but they are rich in carbohydrates and fibers and add significant amounts of micronutrients to the human diet. they are also a source of functional substances, which might benefit one or more physiological functions in the body, besides adequate nutritional effects. functional elements might play a role in improving health and well-being, as well as reducing the risk of the onset of diet-related diseases. the development of vegetable cultivars with greater amounts of these substances is one of the main goals of modern breeding programs. many of these programs, working on different vegetables, are currently underway in brazil and other countries, aiming to improve the amount and variety of carotenoids present in the diet. in the present paper, the main aspects of vegetable crops as functional foods are discussed. the most important achievements of tomato and carrot breeding programs in brazil aiming to improve the amount and types of functional compounds are also presented.
Estimativa indireta de teores de licopeno em frutos de genótipos de tomateiro via análise colorimétrica
Carvalho, Wesley;Fonseca, Maria Esther de N.;Silva, Henoque R. da;Boiteux, Leonardo S.;Giordano, Leonardo de B.;
Horticultura Brasileira , 2005, DOI: 10.1590/S0102-05362005000300026
Abstract: lycopene (c40h56) is a red carotenoid pigment, which naturally accumulates in flowers, fruits and roots of some vegetable and fruit crops. the unique properties of the lycopene molecule make this pigment one of the most powerful antioxidants available. tomato (lycopersicon esculentum mill.) fruits and processed tomato products are the main sources of lycopene in the human diet. there is a growing amount of experimental data indicating that lycopene intake is associated with decreased risk of many types of degenerative and cardiovascular diseases as well as cancers in humans. in addition, lycopene concentration is associated with improved visual perception of tomato products. therefore, there is a strong demand to increase the total amount of lycopene in tomato cultivars for either fresh market or processing. the development of cultivars for improved lycopene content is dependent upon the availability of simple yet accurate methodologies to quantify this carotene in tomato fruits and processed tomato products. the main objective of this work was to estimate the degree of correlation between the total lycopene content in tomato fruits measured spectrophotometrically and the chromaticity values (l*, a* and b*) obtained using a tristimulus colorimeter. colorimetric readings were taken using three sampling strategies: (1) external fruit wall; (2) internal fruit wall and (3) the whole homogenized fruit pulp. for the achromatic component l* (relative darkness or lightness), the obtained r2 values were within 0.63 and 0.82, with the whole homogenized fruit pulp providing the highest correlation. for the chromatic component a* (green to red) the correlation r2 values ranged from 0.79 to 0.82. for the chromatic component b* (blue to yellow) lower yet significant values were observed (r2 between 0.27 and 0.46). however, it was observed that lycopene content was best correlated with the mathematical relationships a*/b* (r2 range from 0.88 to 0.90) and (a*/b*)2 (r2 range from 0.
Dermatite factícia desencadeada pela síndrome de Münchausen
Silva, Náiade Maria Rêgo e;Petrucci, Giannina Wanderley;Palitot, Esther Bastos;Azzouz, Mohamed Arbaqui;Azzouz, Samir de Figueiredo;
Anais Brasileiros de Dermatologia , 2010, DOI: 10.1590/S0365-05962010000100011
Abstract: factitial dermatitis is a condition in which skin lesions are self-inflicted as the result of a psychiatric disorder. the lesions may be presented in various forms and produced by a wide variety of means. this report refers to the case of a patient with factitial dermatitis triggered by munchausen syndrome, which refers to individuals who intentionally provoke physical symptoms in themselves in order to satisfy a need to receive frequent medical attention.
Seroepidemiology of Chagas disease in the Chagas Disease Control Program of the S o Paulo State, Brazil
CARVALHO Maria Esther de
Revista do Instituto de Medicina Tropical de S?o Paulo , 2001,
Publicaciones electrónicas: experiencias y desafíos en Perú
Mogollón, Maria Esther;
Revista Estudos Feministas , 2004, DOI: 10.1590/S0104-026X2004000300021
Abstract: the author explains her experiences on the electronic publication field, and in very important printed publications such as the emblematic fempress. she approaches the use of the technologies of information and communication (tic), that have allowed to share information and contributed to the invigoration and monitoring of actions that propitiate the rendering of information and strengthening of women's nets. by using the tics, women have promoted alliances and articulations, favoring the structuring and construction of proposals, consents and more equal and democratic relationships in the feminist and women movement. the author describes that through the eletronic publications it is generated and exchanged information about the problems that women live; their efforts and advances are exposed, the solidarity is developed, and a great variety of themes is approached. the nets of women grow and get strengthen, and the information generated by them, grows in an exponential way. but these nets are also integrated by poor women, peasants, indigenous, illiterate, women who speak other languages or with discapacity, who cannot access the tics, so it is necessary to look for the ways of reverting this situation.
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