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Search Results: 1 - 10 of 286163 matches for " Maria C. Hernandez-Soriano "
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Protein Bodies in Cotyledon Cells Exhibit Differential Patterns of Legumin-Like Proteins Mobilization during Seedling Germinating States  [PDF]
Jose C. Jimenez-Lopez, Maria C. Hernandez-Soriano
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.412304

Olive (Olea europaea L.) tree is one of the most extensive and important agricultural crop in Mediterranean countries due to its beneficial health and nutritional properties and its high economic value. Currently, olive tree constitutes the sixth most important cultivated plant in the world, spreading from the Mediterranean region of origin to new production areas such as Australia, South and North America and South Africa. However, the mobilization processes of storage materials i.e. reserve proteins during seed germination, which are largely involved in essential physiological process including plant growth and development, remain poorly understood. Morphometric and immunohistochemistry analyses of protein bodies contained in olive seed storage tissues, cotyledon and endosperm, were performed by using different microscopy techniques, including light (bright-field and fluorescence) microscopy and transmission electron microscopy. Furthermore, we used legumin-like proteins (11S-type globulins) as a molecular marker to study the mobilization of reserve proteins from PBs of cotyledons at germinating seedling stages by using immunofluorescence assays. Results demonstrated that cotyledon and endosperm are characterized by distinct PBs populations containing legumin-like proteins, distinctly discriminated by the number of PBs per cell and tissue, size, immunofluorescence and histochemical staining. These features reflect differential PBs biogenesis during development and maturation processes in olive seed tissues endosperm and cotyledon, in relation to proteins (polypeptides) final composition, SSPs processing and/or packaging during seed maturation. Three different mobilization patterns of legumin-like proteins were identified for the first time in cotyledon PBs

Parasitosis intestinales y su relacion con factores socioeconómicos y condiciones de habitat en ni os de Neuquén, Patagonia, Argentina INTESTINAL PARASITOSIS IN RELATION TO SOCIOECONOMIC FACTORS AND HABITAT CONDITIONS IN CHILDREN OF NEUQUéN, PATAGONIA, ARGENTINA
Parasitología latinoamericana , 2005,
Abstract: Se investigó la prevalencia y distribución de parásitos intestinales (PI) en ni os de 2 poblaciones de diferente nivel socioeconómico del área periurbana de la ciudad de Neuquén (Sectores I y II) a fin de evaluar su relación con las condiciones de hábitat y factores socio-económicos. Se procesaron muestras seriadas de materia fecal y de escobillado anal de 126 ni os entre 2 y 14 a os de edad. Se registraron datos acerca de condiciones de hábitat y factores socioeconómicos mediante visitas domiciliarias y encuestas observaciones estructuradas. Se detectó presencia de PI en el 50,7% de los ni os del Sector I (barrio suburbano con adecuadas condiciones sanitarias y nivel socioeconómico medio o medio-bajo) y en el 92,9% de los ni os del Sector II (asentamiento marginal con deficientes condiciones sanitarias y bajo nivel socioeconómico). Se identificaron 7 especies de protozoos intestinales y 4 especies de helmintos. Blastocystis hominis fue la especie más frecuente encontrada en ambas poblaciones. No se encontraron helmintos diferentes de Enterobius vermicularis en el Sector I y la prevalencia de tales especies fue muy baja en el Sector II. Las condiciones de hábitat deficientes y los bajos parámetros socioeconómicos se relacionaron con una mayor prevalencia de PI de transmisión directa como protozoos y E. vermicularis en las poblaciones estudiadas. Sin embargo, aún en ese contexto favorable a la transmisión, las especies parasitarias que requieren estadíos intermedios de maduración en el suelo no encuentran un hábitat adecuado para su diseminación en esta región patagónica The prevalence and distribution of intestinal parasites (IP) were investigated in children from two populations of different socioeconomic level, located in the same area of the city of Neuquén, in order to evaluate their relationship with habitat conditions and socioeconomic factors. Serial samples of faeces and anal scraping of 126 children between 2 and 14 years from two sectors of the suburban area of Neuquen (Sector I and Sector II) were analyzed. Data concerning habitat conditions and socioeconomic parameters were obtained by home visits and an observational structured survey. Presence of IP was detected in 50.7% of children from Sector I (suburban neighborhood with adequate sanitary conditions and middle or middle low socioeconomic level) and in 92.9% from children of Sector II (marginal settlement with poor sanitary conditions and low socioeconomic status). Seven intestinal protozoan and 4 helminth species were identified. Blastocystis hominis was the most frequent species found
Color Fusion of Magnetic Resonance Images Improves Intracranial Volume Measurement in Studies of Aging  [PDF]
Maria del C. Valdes Hernandez, Natalie A. Royle, Michael R. Jackson, Susana Mu?oz Maniega, Lars Penke, Mark E. Bastin, Ian J. Deary, Joanna M. Wardlaw
Open Journal of Radiology (OJRad) , 2012, DOI: 10.4236/ojrad.2012.21001
Abstract: Background: Comparison of intracranial volume (ICV) measurements in different subpopulations offers insight into age-related atrophic change and pathological loss of neuronal tissue. For such comparisons to be meaningful the accuracy of ICV measurement is paramount. Color magnetic resonance images (MRI) have been utilised in several research applications and are reported to show promise in the clinical arena. Methods: We selected a sample of 150 older community-dwelling individuals (age 71 to 72 years) representing a wide range of ICV, white matter lesions and atrophy. We compared the extraction of ICV by thresholding on T2*-weighted MR images followed by manual editing (reference standard) done by an analyst trained in brain anatomy, with thresholding plus computational morphological operations followed by manual editing on a framework of a color fusion technique (MCMxxxVI) and two automatic brain segmentation methods widely used, these last three done by two image analysts. Results: The range of ICV was 1074 to 1921 cm3 for the reference standard. The mean difference between the reference standard and the ICV measured using the technique that involved the color fusion was 2.7%, while it was 5.4% compared with any fully automatic technique. However, the 95% confidence interval of the difference between the reference standard and each method was similar: it was 7% for the segmentation aided by the color fusion and was 7% and 8.3% for the two fully automatic methods tested. Conclusion: For studies of aging, the use of color fusion MRI in ICV segmentation in a semi-automatic framework delivered best results compared with a reference standard manual method. Fully automated methods, while fast, all require manual editing to avoid significant errors and, in this post-processing step color fusion MRI is recommended.
Bacterial Diversity of Marine Seeps in the Southeastern Gulf of Mexico
Maria C. Rosano-Hernandez,Luis C. Fernandez-Linares,B. Xoconostle-Cazares
Pakistan Journal of Biological Sciences , 2009,
Abstract: The diversity of bacterial communities of shallow (≤ 100 m depth) oil seep marine sediments from the Southern Gulf of Mexico was evaluated. The geochemical properties of seep sediments were characterized as well as their microbial diversity in oil seep and control sediments. Bacteria were identified through molecular tools as belonging to the genera Marinobacter, Idiomarina, Marinobacterium, Frauteria and an unknown bacterium. Bacteria might be important components of microbial communities in Total Petroleum Hydrocarbon (TPH)-containing environments, displaying either facultative metabolism or able to grow only in petroleum-containing media. The identification of bacteria in shallow oil seep sediments could be used as indicators of marine hydrocarbons in Southeastern Gulf of Mexico.
The usefulness of mesocosms for ecotoxicity testing with lacertid lizards
Maria José Amaral,Rita C. Bicho,Miguel A. Carretero,Juan C. Sanchez-Hernandez
Acta Herpetologica , 2012,
Abstract: Mesocosms (i.e., outdoor, man-made representations of natural ecosystems) have seldom been used to study the impact of contaminants on terrestrial ecosystems. However, mesocosms can be a useful tool to provide a link between field and laboratory studies. We exposed juvenile lacertid lizards for a period of over one year to pesticides (herbicides and insecticides) in mesocosm enclosures with the intention of validating field observations obtained in a previous study that examined the effects of corn pesticides in Podarcis bocagei. Our treatments replicated field conditions and consisted of a control, an herbicides only treatment (alachlor, terbuthylazine, mesotrione and glyphosate) and an herbicides and insecticide treatment (including chlorpyrifos). We used a multi-biomarker approach that examined parameters at an individual and sub-individual level, including growth, locomotor performance, standard metabolic rate, biomarkers of oxidative stress, esterases and liver histopathologies. Although mortality over the course of the exposures was high (over 60%), surviving individuals prospered relatively well in the mesocosms and displayed a broad range of natural behaviours. The low numbers of replicate animals compromised many of the statistical comparisons, but in general, surviving lizards exposed to pesticides in mesocosm enclosures for over one year, thrived, and displayed few effects of pesticide exposure. Despite the difficulties, this work acts as an important stepping-stone for future ecotoxicology studies using lizards.
Erythromycin-Resistant Streptococcus pyogenes in Argentina
Lopardo,Horacio A.; Hernandez,Claudia; Vidal,Patricia; Vazquez,Miryam; Rosaenz,Liliana; Rubinstein,Gabriela; Smayevsky,Jorgelina; Tokumoto,Marta; Fernandez Lausi,Adriana; Daher,Omar; Kaufman,Sara; Soriano,Silvia V.; Brasili,Susana; Bottiglieri,Marina; Carranza,Maria Cristina;
Medicina (Buenos Aires) , 2004,
Abstract: erythromycin (ery) resistance in streptococcus pyogenes has recently emerged as a problem of growing concern all through the world. we are presenting the comparison of results of the continuous surveillance of erythromycin resistance in s. pyogenes performed since 1989 in the hospital de pediatría j.p.garrahan of buenos aires city, with independently observed rates in other five centers of buenos aires and seven centers of six other argentinian cities, obtained between 1999 and 2001. a significant increase of erythromycin resistance was observed among s. pyogenes isolated in the hospital garrahan (6.6% in 1998-1999 to 9.9% in 2000). similar trends were also detected in other centers of other argentinian cities when recent data were compared to results of a multicenter study performed in 1995. however, lower rates of resistance were recorded in mendoza, cipolletti and neuquén in comparison with data of 1995, 1998 and 1998 respectively. the reason of such decreasing resistance rates deserves to be investigated. the average of ery-resistance rates obtained in the surveyed centers was 6.7% (range 0.5 - 14.1%). control of antimicrobial use should be performed to warrant the future effectiveness of macrolide antibiotics regarding the positive association between use and resistance. these results also suggest that susceptibility tests for macrolides should be performed whenever s. pyogenes is isolated in argentina.
Períodos de interferência de maria-pretinha sobre tomateiro industrial
Hernandez, Daniel D;Alves, Pedro Luís CA;Pavani, Maria do Carmo MD;Parreira, Mariana C;
Horticultura Brasileira , 2007, DOI: 10.1590/S0102-05362007000200014
Abstract: in order to evaluate the effects of nightshade coexistence on processing tomato yield, hybrid heinz 9992, an experiment was carried out consisting of two sets of treatments: in the first set, the crop was kept weed free (60 thousands plants ha-1) from transplanting to 15; 30; 45; 60; 75; 90; 105 days and 120 (harvest) days after transplanting (dat); while in the second set, the crop was kept in competition with the nightshade from transplanting until the same dat described for the set of treatments. a randomized block design trial, with three replications and four-row plots was used. height, leaf area, and aerial part dry matter of nightshade was obtained out of 10-plant samples. nightshade leaf area increased until 75 days of tomato coexistence (1,588 dm2 plant-1); height until 60 days of coexistence (85 cm), and the leaf dry biomass until 120 days of coexistence (31.7 g plant-1). the highest (108.6 tons ha-1 or 87.6 % of total production) and lowest (14.2 tons ha-1 or 59%) ripe tomato yields were observed when the coexistence between tomato and nightshade occurred only in the first 15 days or in the whole crop cycle, respectively. in these treatments, the average ripe fruit weights were 58.7 and 38.0 g, respectively. each cm2 of increase in the nightshade leaf area caused a reduction of 0.04 tons ha-1 (r2 = 0.90) on the ripe tomato yield; each cm of increase in the nightshade height reduced the ripe tomato yield in 0.82 tons ha-1 (r2=0.78); and each gram of increase in the nightshade aerial part dry matter reduced the ripe tomato yield in 2.84 tons ha-1 (r2=0.97). the period before interference, allowing up to 5% reduction in tomato yield, was 27 dat; the total period for preventing interference was 46 dat; and the critical period for preventing interference was between 27 and 46 dat.
Suplementa??o de iogurte de soja com frutooligossacarídeos: características probióticas e aceitabilidade
Hauly, Maria Célia de Oliveira;Fuchs, Renata Hernandez Barros;Prudencio-Ferreira, Sandra Helena;
Revista de Nutri??o , 2005, DOI: 10.1590/S1415-52732005000500004
Abstract: objective: the probiotic characteristics of the microorganisms (lactobacillus bulgaricus and streptococcus thermophillus) used to produce soy yogurt supplemented with prebiotic fructooligosaccharides were evaluated and also the physicochemical and microbiological characteristics and acceptance of the product. methods: the formulation containing powdered soy extract, supplemented with fructooligosaccharides (oligofructose and inulin), was fermented for 6 hours (42oc) and then characterized and compared to a soy yogurt formulation made without supplementation. results: the starter used to prepare the yogurts (lactobacillus bulgaricus and streptococcus thermophillus) showed resistance to bile and to acid. the prebiotics maintained the viability of the lactic acid bacteria at levels higher than necessary to characterize the product as a probiotic food, up to the 28th day of storage. the final ph value of the supplemented yogurt was 4.63, the acidity 0.37% and the acceptance 71.20%. the supplemented yogurt showed greater viscosity, cohesiveness and adhesiveness than the non-supplemented product and was less hard. conclusion: lactobacillus bulgaricus and streptococcus thermophillus are probiotics and fructooligosaccharides are ingredients that maintain the viability of lactic acid bacteria in soy yogurt, giving rise to a product with good acceptability.
Desenvolvimento de um inventário de levantamento das dificuldades da decis o profissional
Primi Ricardo,Hernandez Munhoz Alícia Maria,Bighetti Cássia Aparcida,Di Nucci Eliane Porto
Psicologia: Reflex?o e Crítica , 2000,
Abstract: Apresenta-se o desenvolvimento de um inventário baseado em um modelo da indecis o profissional que se apoia nas teorias da tomada de decis o. Aplicou-se o inventário em 227 alunos, 52,8% de uma escola estadual e 47,2% de uma escola particular, a maioria de 14 a 17 anos, freqüentando a oitava serie do Ensino Fundamental e segunda e terceira série do Ensino Médio. A análise fatorial indicou a presen a de 17 fatores primários hierarquicamente agrupados em quatro fatores mais amplos: a) inseguran a e falta de informa o (Alfa 0,89); b) ênfase no busca de prestígio e retorno financeiro (Alfa 0,79); c) imaturidade para a escolha (Alfa 0,70); e, d) conflitos com pessoas significativas (Alfa 0,71). Uma ANOVA indicou que os alunos de escolas estaduais tendem a privilegiar o aspecto econ mico e os alunos mais jovens apresentam um maior grau de imaturidade, atestando a validade do instrumento.
The Role of Prolactin in the Evolution of Prostate Cancer  [PDF]
Maria Elena Hernandez, Michael J. Wilson
Open Journal of Urology (OJU) , 2012, DOI: 10.4236/oju.2012.223034
Abstract: Today there is significant information indicating an effect of prolactin on the prostate gland. It has been shown to be involved in mechanisms leading to the synthesis of some proteins such as PSA and cathepsin D, synthesis of citrate and accumulation of zinc. Also, at the behavioral level, prolactin is known to control some aspects of reproduction, including documentation on the physiology of the prostate and the possibility to trigger pathologies in this sex gland. Although the later still is not clear, there is a correlation between the level of prolactin and the presence of prostate pathologies. Thus, the aim of this review is to show how prolactin is involved in the progression of some pathologies of this male sexual gland.
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