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Search Results: 1 - 10 of 71025 matches for " Maria Benevolo "
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Anal cytological abnormalities and epidemiological correlates among men who have sex with men at risk for HIV-1 infection
Donà Maria,Benevolo Maria,Vocaturo Amina,Palamara Guido
BMC Cancer , 2012, DOI: 10.1186/1471-2407-12-476
Abstract: Background The incidence of anal cancer, a Human Papillomavirus (HPV)-related neoplasia, has been increasing in recent decades, mainly in men who have sex with men (MSM). Cytological changes of the anal epithelium induced by HPV can be detected through an anal pap smear. This study aimed to evaluate the prevalence and epidemiological correlates of anal cytological abnormalities among relatively young MSM at risk for HIV-1 infection, to help clarify whether or not this population deserves further investigation to assess the presence of anal cancer precursor lesions. Methods MSM were recruited among attendees of a large STI clinic for a HIV-1 screening program. Anal samples, collected with a Dracon swab in PreservCyt, were used both for liquid-based cytology and HPV testing by the Linear Array HPV Genotyping Test. Data regarding socio-demographic characteristics and sexual behavior were collected in face-to-face interviews. Results A total of 346 MSM were recruited (median age 32 years). Overall, 72.5% of the individuals had an anal HPV infection, with 56.1% of them being infected by oncogenic HPV genotypes. Anal cytological abnormalities were found in 29.8% of the cases (16.7% ASC-US and 13.1% L-SIL). Presence of ASC-US+ was strongly associated with infection by any HPV type (OR=4.21, 95% CI: 1.97-9.23), and particularly by HPV 16 and/or 18 (OR=5.62, 95% CI: 2.33-13.81). A higher proportion of ASC-US+ was found in older MSM, in those with a higher number of lifetime partners and in those with a history of ano-genital warts. However, none of these variables or the others analyzed showed any significant association with abnormal cytological findings. Conclusions The presence of anal cytological abnormalities in about one third of the recruited MSM and their strong association with HPV infection, in particular that caused by HPV 16 and/or 18, might provide a further complement to the data that now support the introduction of HPV vaccination among MSM to protect them from the development of HPV-associated diseases. Additional studies are needed to determine whether and how screening for anal cancer precursor lesions should be performed in younger MSM.
Identification of Episomal Human Papillomavirus and Other DNA Viruses in Cytological Anal Samples of HIV-Uninfected Men Who Have Sex with Men
Maria Gabriella Donà, Francesca Paolini, Maria Benevolo, Amina Vocaturo, Alessandra Latini, Amalia Giglio, Aldo Venuti, Massimo Giuliani
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0072228
Abstract: To date, there have been only few studies that investigated integration of anal Human Papillomavirus (HPV). Most of them were conducted on HIV-infected individuals and mainly analyzed samples from high-grade lesions and invasive cancer. We aimed to investigate HPV physical status in HIV-negative men who have sex with men (MSM) with a detectable anal HPV infection, irrespective of the presence of lesions. We also sought to explore the presence of other circular DNA viruses in the anal region. Study participants were attendees of an STI screening program, which were also screened for anal HPV infection and cytological abnormalities. HPV physical status was assessed using multiply-primed RCA. HPV16-positive samples were also analyzed using E2/E6 multiplex PCR, qRT-PCR and APOT assay. RCA and virus-specific PCR were employed to investigate the presence of other DNA viruses. Anal HPV infection was detected in 76.9% of the 230 MSM enrolled. The anal cytological reports were: 129 NILM, 37 ASC-US and 28 L-SIL (36 samples were inadequate for interpretation). HPV physical status was evaluated in the 109 anal specimens that harbored one or two different HPV genotypes. Integration was observed only in one HPV16-positive sample (0.9%), in which integrate-derived viral transcripts of type B were detected. Integration occurred in chromosome 14 q. In 22 of the 53 (41.5%) mucosal HPV-negative samples, RCA restriction results would seem to indicate the presence of circular DNA viruses. Indeed, cutaneous HPV (4 samples), MCPyV (5 samples) and TTV (4 samples) were detected. In conclusion, anal HPV integration was rarely evidenced in HIV-uninfected MSM with no or mild anal cytological abnormalities, although the integration rate may have been underestimated because of the limitations of the employed assays. Other DNA viruses were detected in the anal samples of these individuals, although the significance of this occurrence needs to be assessed.
Influence of pH to Increase Grafting Degree into Fluoropolymers  [PDF]
Giovanni González-Pérez, Ademar Benevolo Lugao
Open Journal of Polymer Chemistry (OJPChem) , 2017, DOI: 10.4236/ojpchem.2017.73004
Poly(ethylene-alt-tetra-fluoroethylene (ETFE) and poly(tetrafluoroethylene-cohexafluoropropylene) (FEP) were pre-irradiated under air using a Co60 gamma source to graft styrene at low pH. Grafting copolymers were tuned by study of different parameters (monomer, reaction time, temperature, and pH with addition of sulfuric acid (H2SO4)). The maximum degree of grafting was 80% and 40% for ETFE and FEP respectively at dose 2 kGy. Influence of low pH in grafting degree by adding sulfuric acid was studied. Grafting degree was examined by infrared (FTIR-ATR), differential scanning calorimetry (DSC) and swelling behavior analysis after sulfonation process.
Claspin as a biomarker of human papillomavirus-related high grade lesions of uterine cervix
Maria Benevolo, Antonio Musio, Amina Vocaturo, Maria Gabriella Donà, Francesca Rollo, Irene Terrenato, Mariantonia Carosi, Edoardo Pescarmona, Giuseppe Vocaturo, Marcella Mottolese
Journal of Translational Medicine , 2012, DOI: 10.1186/1479-5876-10-132
Abstract: In order to investigate whether claspin immunoreactivity is related to the lesion severity and High-Risk (HR) HPV infection, we analyzed claspin expression by immunohistochemistry in a series of cervical biopsies which represent the steps occurring during cervical carcinogenesis (normal tissues, Cervical Intraepithelial Neoplasias 1, 2 and 3, Squamous Cell Carcinomas). All patients also had a cervico-vaginal sample for HPV testing, collected immediately before the colposcopy-guided biopsy. The HR-HPV DNA detection was performed by the HR-HPV Hybrid Capture 2 test. HPV genotyping was performed using the Linear Array HPV Genotyping Test.Our results evidenced a constant and significant increase of the rate of claspin positivity from the normal tissues to carcinomas (pχ2trend?<?0.0001). In fact, the normal tissues displayed either no or faint claspin immunoreactivity, whereas a moderate/high positivity was observed in 16% of the CIN1, 76% of the CIN2, 87.5% of the CIN3 and 93.3% of the cancers. Moreover, we found a statistically significant correlation between claspin expression and HR-HPV infection (pχ2?<?0.0001), irrespective of the genotype. Finally, we demonstrated the feasibility of claspin immunostaining in cervical cytology.Our findings indicate that in vivo claspin expression is significantly related to HR-HPV infection and lesion grade both in histological and cytological samples. Therefore, the analysis of claspin expression could be clinically relevant in the diagnosis of HPV-related cervical lesions, in particular when applied to cervico-vaginal cytology. Moreover, giving information on the proliferation rate of each lesion, claspin immunostaining may contribute to the evaluation of progression risk, thus being helpful in patient management. Nevertheless, only large prospective studies may clarify the true clinical usefulness of claspin expression in distinguishing lesions with different progression potential.
High expression of HLA-E in colorectal carcinoma is associated with a favorable prognosis
Maria Benevolo, Marcella Mottolese, Elisa Tremante, Francesca Rollo, Maria Grazia Diodoro, Cristiana Ercolani, Isabella Sperduti, Elisa Lo Monaco, Maurizio Cosimelli, Patrizio Giacomini
Journal of Translational Medicine , 2011, DOI: 10.1186/1479-5876-9-184
Abstract: Taking advantage of MEM-E/02, a recently characterized antibody to denatured HLA-E molecules, HLA-E expression was assessed by immunohistochemistry on an archival collection (formalin-fixed paraffin-embedded) of 149 colorectal primary carcinoma lesions paired with their morphologically normal mucosae. Lymphoid infiltrates were assessed for the expression of the HLA-E-specific, inhibitory, non-rearranging receptor NKG2A.High HLA-E expression did not significantly correlate with the expression of classical HLA-B and HLA-C molecules, but it did correlate with high expression of its preferential ligand donor HLA-A. In addition, it correlated with lymphoid cell infiltrates expressing the inhibitory NKG2A receptor, and was an independent predictor of good prognosis, particularly in a subset of patients whose tumors express HLA-A levels resembling those of their paired normal counterparts (HLA-A). Thus, combination phenotypes (HLA-Elo-int/HLA-AE and HLA-Ehi/HLA-AE) of classical and non-classical class I HLA molecules mark two graded levels of good prognosis.These results suggest that HLA-E favors activating immune responses to colorectal carcinoma. They also provide evidence in humans that tumor cells entertain extensive negotiation with the immune system until a compromise between recognition and escape is reached. It is implied that this process occurs stepwise, as predicted by the widely accepted 'immunoediting' model.Human Leukocyte Antigen (HLA)-E is a cell surface, non-classical Major Histocompatibility class I molecule recognized by immune receptors expressed by cytotoxic T lymphocytes (CTLs), Natural Killer (NK) cells, and the more recently described subset of NK-CTLs. These receptors are either inhibitory or activating [1-3].Inhibition, on the one hand, results from the engagement of the NKG2A receptor with HLA-E heavy chains that have been stabilized upon heterotrimeric assembly with their light chain subunit, called β2-microglobulin (β2m), and peptide ligands de
Human Papillomaviruses, p16INK4a and Akt expression in basal cell carcinoma
Francesca Paolini, Angelo Carbone, Maria Benevolo, Vitaliano Silipo, Francesca Rollo, Renato Covello, Paolo Piemonte, Pasquale Frascione, Rodolfo Capizzi, Caterina Catricalà, Aldo Venuti
Journal of Experimental & Clinical Cancer Research , 2011, DOI: 10.1186/1756-9966-30-108
Abstract: The expression of p16INK4a and Akt, by immunohistochemistry, and the HPV DNA, by nested PCR, were investigated in each sample.No correspondence of HPV types between BCC and swab samples was found, whereas a correspondence between perilesional skin and BCC was ascertained in the 16,7% of the patients. In BCC, 16 different types of beta HPV were found and the most frequent types were HPV107 (15,4%), HPV100 (11,5%) and HPV15 (11,5%) all belonging to the beta HPV species 2. Immunohistochemistry detected significant p16INK4a expression in almost all tumor samples (94,3%) with the highest percentages (> 30%) of positive cells detected in 8 cases. A statistically significant (p = 0,012) increase of beta HPV presence was detected in p16INK4a strongly positive samples, in particular of species 2. pAkt expression was detected in all tumor samples with only 2 cases showing rare positive cells, whereas Akt2 expression was found in 14 out of 35 BCC (40%); in particular in HPV positive samples over-expressing p16INK4a.Our data show that p16INK4a and pAkt are over-expressed in BCC and that the high expression of p16INK4a and of Akt2 isoform is often associated with the presence of beta-HPV species 2 (i.e. HPV 15). The association of these viruses with the up-regulation of p16INK4a and Akt/PI3K pathway suggests that in a subtype of BCC these viruses may exert a role in the carcinogenesis or in other, still undefined, biological property of these tumors. If this particular type of BCC reflects a different biology it will remain undisclosed until further studies on a larger number of samples will be performed.The family of the Human Papillomaviruses (HPVs) comprises more than 120 different genotypes, 112 (HPV1 to HPV112) of which were characterized after cloning and sequencing of their genomes [1-3]. Currently, HPVs are classified into five genera: Alpha(α)-, Beta (β)-, Gamma(γ)-, Mu(μ)- and Nu(ν)- papillomavirus, according to their genomic DNA sequence [1]. The phylogeny of PVs indi
Prognostic Value of HPV E6/E7 mRNA Assay in Women with Negative Colposcopy or CIN1 Histology Result: A Follow-Up Study
Paolo Giorgi Rossi, Maria Benevolo, Amina Vocaturo, Donatella Caraceni, Lucia Ciccocioppo, Antonio Frega, Irene Terrenato, Roberta Zappacosta, Deborah French, Sandra Rosini
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0057600
Abstract: Pap test, and especially HPV DNA test, identify a large group of women who do not have any clinically relevant lesions, i.e., CIN2+ (Cervical Intraepithelial Neoplasia grade 2 or worse), but who are at greater risk of getting lesions in the future. The follow up of these women needs new biomarkers with prognostic value. The objective of this study is to evaluate the prognostic value of E6/E7 mRNA over-expression assay (PreTect HPV-Proofer, Norchip) for 5 HR-HPV types (16, 18, 31, 33, and 45) for progression to CIN2+ after a negative colposcopy. This prospective study, conducted at four Italian centres, enrolled 673 women with either a negative colposcopy or a negative or CIN1 histology. The clinical end-point was histological confirmation of CIN2+. Women were classified at baseline according to mRNA results and managed according to local colposcopy protocols. At least one conclusive follow-up test was obtained for 347 women (25 months average lapse since recruitment, range 5–74). Only seven CIN2+ were detected during follow up, three among the 82 women positive for mRNA at baseline, two among the 250 negative (Fisher exact test, p = 0.02), and two among the 12 with an invalid test. Absolute CIN2+ risk was 6.7/1,000 person/years in the whole cohort. The absolute CIN2+ risk was 18.4/1,000 person/years and 3.6/1,000 person/years in mRNA-positive and mRNA-negative women, respectively. In conclusion, E6/E7 mRNA over-expression appears to be a good candidate as a prognostic biomarker to manage HR-HPV DNA-positive women with negative colposcopy or histology, particularly in order to decrease follow-up intensity in those who are negative.
Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems
Patrizia Galeffi, Alessio Lombardi, Immacolata Pietraforte, Flavia Novelli, Monica Di Donato, Maria Sperandei, Andrea Tornambé, Rocco Fraioli, Aline Martayan, Pier Natali, Maria Benevolo, Marcella Mottolese, Francisco Ylera, Cristina Cantale, Patrizio Giacomini
Journal of Translational Medicine , 2006, DOI: 10.1186/1479-5876-4-39
Abstract: Herein, we describe a multi-platform approach for the production of recombinant Single chain Fragments of antibody variable regions (ScFvs) to ErbB-2 that involves their functional expression in (a) bacteria, (b) transient as well as stable transgenic tobacco plants, and (c) a newly developed cell-free transcription-translation system.An ScFv (ScFv800E6) was selected by cloning immunoglobulin sequences from murine hybridomas, and was expressed and fully functional in all the expression platforms, thereby representing the first ScFv to ErbB-2 produced in hosts other than bacteria and yeast. ScFv800E6 was optimized with respect to redox synthesis conditions. Different tags were introduced flanking the ScFv800E6 backbone, with and without spacer arms, including a novel Strep II tag that outperforms conventional streptavidin-based detection systems. ScFv800E6 was resistant to standard chemical radiolabeling procedures (i.e. Chloramine T), displayed a binding ability extremely similar to that of the parental monovalent Fab' fragment, as well as a flow cytometry performance and an equilibrium binding affinity (Ka approximately 2 × 108 M-1) only slightly lower than those of the parental bivalent antibody, suggesting that its binding site is conserved as compared to that of the parental antibody molecule. ScFv800E6 was found to be compatible with routine reagents for immunohistochemical staining.ScFv800E6 is a useful reagent for in vitro biochemical and immunodiagnostic applications in oncology, and a candidate for future in vivo studies.The ErbB family of receptor tyrosine kinases includes four members (ErbB-1 or HER-1 through ErbB-4 or HER-4) that signal upon engagement in combinatorial dimeric receptor combinations. ErbB-2 is the shared (and preferred) heterodimerization partner, and acts as the master coordinator and integrator of signaling amplification. Aberrant ErbB signaling is causally involved in the pathogenesis of human tumors including astrocytomas, head and ne
Human Papilloma Virus prevalence and type-specific relative contribution in invasive cervical cancer specimens from Italy
Luciano Mariani, Núria Monfulleda, Laia Alemany, Enrico Vizza, Ferdinando Marandino, Amina Vocaturo, Maria Benevolo, Beatriz Quirós, Belén Lloveras, Jo Klaustermeier, Wim Quint, Silvia de Sanjosé, F Xavier Bosch
BMC Cancer , 2010, DOI: 10.1186/1471-2407-10-259
Abstract: To describe the prevalence of HPV and the HPV type-specific distribution in ICC cases identified in Rome, Italy.140 paraffin embedded tissue blocks of primary ICC diagnosed between 2001 and 2006 were identified at the Regina Elena Cancer Institute in Rome (Italy). HPV was detected through amplification of HPV DNA using SPF-10 HPV broad-spectrum primers followed by DEIA and then genotyping by LiPA25 (version 1).134 cases were considered suitable for HPV DNA detection after histological evaluation; and overall, 90.3% (121/134) HPV prevalence was detected. 111 cases had a single HPV type, 4 cases had an uncharacterized type (HPVX) and 6 cases had multiple HPV infections. The five most common single HPV types among positive cases were: HPV16 (71/121; 58.7%), HPV18 (12/121; 9.9%), HPV31, HPV45 and HPV58 (5/121; 4.1% each). 2 (1.5%) of the single infections and 2 (1.5%) of the multiple infections contained low risk types. Statistically significant differences in the relative contribution of HPV18 were found when comparing squamous cell carcinomas with adenocarcinomas.HPV16 and HPV18 accounted for almost 70% of all the HPV positive ICC cases. The study provides baseline information for further evaluation on the impact of recently introduced HPV vaccines in Italy.Cervical cancer is a serious public health problem representing the 2nd most common cancer diagnosed among women worldwide. Around 493,000 new cases and 274,000 deaths from this disease were estimated in 2002 [1]. In this study we analyse and present data regarding Human Papillomavirus (HPV) in invasive cervical cancer (ICC) specimens from Italy, a country whose population consist of 25.94 million [2] women at risk of developing ICC (15 y.o. or older). The age-standardized incidence rate of cervical cancer in Italy has been estimated at 8.1 new cases per 100,000 women annually [1]. Current estimates indicate that 3,418 women are diagnosed with ICC every year and 1,186 die from the disease, ranking the 10th most fre
In the Shadow of the Serbian Paramilitary Units: Narrative Patterns about the Role of Paramilitary Units in Former Yugoslav Conflict  [PDF]
Maria Vivod
Advances in Anthropology (AA) , 2013, DOI: 10.4236/aa.2013.31004
This article1 offers several basic data about Serbian paramilitary units employed in the armed conflict of the 1990’s during the decomposition of the former Yugoslavia, with the goal to depict the transformation of the representation of the paramilitary unit members and their leaders essentially through Serbian media. Once, at the beginning of the conflicts, represented as the saviors, protectors of the Serbian nation, ever present as main figures of the public life of the 90s—they have fallen into oblivion after a decade. Their most notorious leaders are either dead or in prison. After the fall of the Milo?evi? regime (2000) their involvement in war crimes, looting and genocide reached Serbia. From being divinized gradually they become diabolized. The contemporary events and happenings in Serbia are explained in the light of the past of these paramilitary units.
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