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Search Results: 1 - 10 of 77998 matches for " Maria Aparecida Fernandez "
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Construction of Vector for Functional Analysis of the Intrinsically Bent DNA in the ACE3 Replicator from Drosophila melanogaster  [PDF]
Douglas Vinícius Bassalobre de Freitas, Fabrícia Gimenes, Adriana Fiorini, Fabiana dos Santos Rando, Quirino Alves de Lima Neto, Maria Aparecida Fernandez
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.41002

DNA replication is a crucial process for species survival, nevertheless it is not clear which factors define origin selection in multicellular eukaryotes. Developmental gene amplification systems, such as the one described during ovarian follicles development in Drosophila melanogaster, are useful tools for studying of DNA replication process in these organisms. We previously described that the well characterized third chromosome amplified domain of D. melanogater displays three intrinsically bent DNA sites: b1, localized at an amplification control element (ACE3), b2 and b3, both localized at the preferential origin ori-β. This proposal aimed to construct a Drosophila transformation vector, which contains a short deletion at the ACE3, in order to reduce the intrinsically bent DNA site b1, and analyze the functional role of this site in the gene amplification process. Through a series of cloning steps, we obtained a Big Parent vector derivative, containing a deletion at the positions 176-

Genome size of three Brazilian flies from the Sciaridae family
Saccuti, Cecília Ferreira;Soares, Maria Albertina de Miranda;Falco, José Ricardo Penteado;Fernandez, Maria Aparecida;
Genetics and Molecular Biology , 2005, DOI: 10.1590/S1415-47572005000500015
Abstract: we determined the genome size of the brazilian sciarid flies bradysia hygida, rhynchosciara americana and trichosia pubescens (diptera: sciaridae) using absorbance measurements of feulgen-stained nuclei belonging to these species (and chicken erythrocytes as a standard) to calculate the amount of dna in picograms (pg) and the number of base pairs (bp), or c-value, for each of these species. the c-values were: 3 x 108 bp (0.31 pg) for b. hygida; 3.6 x 108 bp (0.37 pg) for r. americana; and 1 x 109 bp (1.03 pg) for t. pubescens. the sciarids investigated in this work had considerably higher c-values than the average for previously described dipteran species, including d. melanogaster.
Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells
Polinarski, Celso Aparecido;Silva, José Luis da Concei??o;Mikami, Liya Regina;Fernandez, Maria Aparecida;
Brazilian Archives of Biology and Technology , 2005, DOI: 10.1590/S1516-89132005000300004
Abstract: a protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using lis detergent is reported in this work. analysis was carried out using fluorescence and confocal laser scan microscopy. the extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. the recovered nuclear halos from bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles.
Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus
Gandra, Eliezer Avila;Fernandez, Maria Aparecida;Silva, Jorge Adolfo;Silva, Wladimir Padilha da;
Ciência e Tecnologia de Alimentos , 2011, DOI: 10.1590/S0101-20612011000400019
Abstract: the enterotoxigenic species staphylococcus aureus, s. hyicus and s. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. this study aimed at the development of a multiplex pcr (mpcr) for the identification of s. aureus, s. intermedius and s. hyicus using the nuc gene as the target sequence. the results obtained suggest that the set of primers used was specific for the three species of staphylococcus evaluate with a detection limit of 102 cfu.ml-1.
Low Genetic Polymorphism at the Cytochrome C Oxidase I in Silkworm Strains of the Brazilian Germplasm Bank  [PDF]
Ver?nica Aureliana Fassina, Thaís Souto Bignotto, Roxelle Ethienne Ferreira Munhoz, Bruno Fulan, Juliana Pereira Bravo, Laura B. Garay, Rafaela Bespalhuk, Claudia Regina das Neves Saez, Naiara Climas Pereira, Graziele Milani Pessini, Maria Aparecida Fernandez
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.43021

Nucleotide sequences have been used to distinguish species and specimens for many years. More recently, the use of a partial sequence of 650 bp of the cytochrome c oxidase I, COI mitochondrial gene, has been proposed for species identification, known as DNA barcodes. In this work, a short sequence of the DNA barcode is described—approximately 250 bp, named as “DNA mini-barcode”—to molecularly identify different silkworm strains maintained at the unique public Germplasm Bank of Bombyx mori, at the Universidade Estadual de Maringá, UEM, Brazil. Analysis revealed no significant differences among the silkworm strains. The phylogenetic tree obtained by the neighbor-joining method and K2P distance, in which specimens of B. mandarina were used as outgroup, clustered all the specimens of B. mori in a unique clade. Genetic variability detect within B. mori was low or nonexistent. In conclusion, the partial region of 250 bp of the mitochondrial gene COI herein analyzed may not be efficient to discriminate silkworm strains from the UEM Germplasm Bank of Bombyx mori.

New Technique to Produce Large Amount of Flat Silk by Biospinning  [PDF]
Laura Beatriz Garay, Andrea Nembri, Alana Lucia Oro, Ver?nica Aureliana Fassina, Cláudia Regina das Neves Saez, Alex Sandro Chiarello, Naiara Climas Pereira, Graziele Milani Pessini, Roxelle Ethienne Ferreira Munhoz, Maria Aparecida Fernandez
Agricultural Sciences (AS) , 2014, DOI: 10.4236/as.2014.514159
Abstract: Beyond the production of silk thread, there are several studies showing that the silk is a great biomaterial for surgical sutures and grafts. This paper shows a new technique to produce silk thread changing the natural cycle of silk production, which is the production of cocoons. This new method has the purpose of producing a silk fabric free of impurities, through flat surfaces. Six different surfaces were tested: Glass, Formica Surface, Steel and Zinc Sheets, Cotton tissue and Burlap Bag. The first five surfaces had not presented enough larvae alive for statistical analysis, because there were several damages in silkworms larvae that resulted in mortality and low silk production. On the other hand, the burlap bag surface presented good results for web construction by biospinning and its use was indicated for silk industry focused on biomaterials. The present study suggested the potential of naturally biospun web, using Bombyx mori, to develop a new technique to produce silk thread matrices that will have several applications at the industry and production of biomedical materials.
Sequence and analysis of the mitochondrial DNA control region in the sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)
Bravo, Juliana Pereira;Felipes, Joice;Zanatta, Daniela Bertolini;Silva, José Luis da Concei??o;Fernandez, Maria Aparecida;
Brazilian Archives of Biology and Technology , 2008, DOI: 10.1590/S1516-89132008000400004
Abstract: this study aimed at the sequence and analysis of the mtdna control region (cr) of the diatraea saccharalis. the genome pcr amplification was performed using the complementary primers to the flanking regions of bombyx mori cr mitochondrial segment. the sequencing revealed that the amplified product was 568 bp long, which was smaller than that observed for b. mori (725 bp). within the amplified segment, a sequence with 338 nucleotides was identified as the control region, which displayed a high at content (93.5%). the d. saccharalis mtdna cr multiple sequence alignment analysis showed that this region had high similarity with the lepidoptera cydia pomonella.
Evaluation of the toxic effect of insecticide chlorantraniliprole on the silkworm Bombyx mori (Lepidoptera: Bombycidae)  [PDF]
Roxelle Ethienne Ferreira Munhoz, Thaís Souto Bignotto, Naiara Climas Pereira, Cláudia Regina das Neves Saez, Rafaela Bespalhuk, Ver?nica Aureliana Fassina, Graziele Milani Pessini, Mayarha Patrícia Dequigiovanni Baggio, Lucinéia de Fátima Chasko Ribeiro, Rose Meire Costa Brancalh?o, Shunsuke Mizuno, Willian Shigeaki Aita, Maria Aparecida Fernandez
Open Journal of Animal Sciences (OJAS) , 2013, DOI: 10.4236/ojas.2013.34051

The silkworm Bombyx mori feeds exclusively on mulberry leaves and is highly sensitive to pesticides in general. Although mulberry plantations are free of agrochemicals, pesticide drift can occur. Chlorantraniliprole, a novel insecticide of the anthranilic diamides class, has been used to control pests in field crops. In this study, we investigated the biological effects of different concentrations of chlorantraniliprole on B. mori silkworm commercial Brazilian hybrids. To evaluate the toxicity of chlorantraniliprole, bioassays were carried out and data on the lethal concentrations, symptomatology, morphology and variables of silk production were collected. Results indicated that B. mori is extremely sensitive to chlorantraniliprole, even in low concentrations. The highest silkworm mortality rates were observed in the two highest chlorantraniliprole concentrations, 0.2 and 0.1 ppm. Although lower chlorantraniliprole concentrations did not cause death of all the silkworm larvae, various symptoms of toxicity were observed: feeding cessation, regurgitation, late development and incomplete ecdysis. Such symptoms reflect the morphological changes we observed in the midgut epithelium, which affected nutrient uptake and metabolism, and even the production of cocoons. Exposed larvae also produced thin-shelled cocoons, which constitutes a serious economic problem because this type of cocoon is not useful for the silk industry. The results provided herein confirm the toxicity of chlorantraniliprole in silkworm larvae. Therefore, we strongly suggest that, competent authorities of the National Health Surveillance Agency, in pesticide management should take measures to reduce or eliminate the use of chlorantraniliprole in areas nearest to silkworm cultivation.

Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori  [PDF]
Cláudia Regina das Neves Saez, Roxelle Ethienne Ferreira Munhoz, Naiara Climas Pereira, Thaís Souto Bignotto, Ver?nica Aureliana Fassina, Graziele Milani Pessini, Laura Beatriz Garay, Lucinéia de Fátima Chasko Ribeiro, Rose Meire Costa Brancalh?o, Maria Aparecida Fernandez
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.45034

This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1; 83% for C25, C75 and C24 strains; 66% for KR01; 50% for M11-A; 33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.

Spanish Samian ware: fundamentals and references
Maria J. Fernandez Fonseca
Papers from the Institute of Archaeology , 1995, DOI: 10.5334/pia.77
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