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Search Results: 1 - 10 of 146623 matches for " Marcia B. Aguila "
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Hepatic Adverse Effects of Fructose Consumption Independent of Overweight/Obesity
Alini Schultz,Debora Neil,Marcia B. Aguila,Carlos A. Mandarim-de-Lacerda
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141121873
Abstract: The chronic intake of fructose has been linked to insulin resistance, obesity, dyslipidemia and nonalcoholic fatty liver disease (NAFLD), which in turn, may progress to nonalcoholic steatohepatitis (NASH). We aimed to evaluate the magnitude of the effects of the chronic consumption of high-fructose (HFr) and high fat (HF) alone or combined. Four groups of male mice were fed different diets for 16 weeks: standard chow (9% fat: SC), HF diet (42% fat), HFr diet (34% fructose) and HF/HFr diet (42% fat, 34% fructose). The food intake was not different among the groups, and the body mass was not greater in the HFr group than in the SC group. The homeostasis model assessment for insulin resistance (HOMA-IR), as well as plasmatic total cholesterol and triglycerides were greater in the groups HF, HFr, and HF/HFr group than in the SC group. We observed in the groups HF, HFr and HF/HFr, compared to the group SC, nonalcoholic fatty liver disease (NAFLD) with a predominance of lipogenesis mediated by SREBP-1c and PPAR-γ, and a reduction of the oxidation mediated by PPAR-α. We also observed an increase in gluconeogenesis mediated by the GLUT-2 and the PEPCK. Importantly, we identified areas of necroinflammation indicating a transition from NAFLD to nonalcoholic steatohepatitis in the HFr and HF/HFr groups. This study is relevant in demonstrating that fructose consumption, even in the absence of obesity, causes serious and deleterious changes in the liver with the presence of the dyslipidemia, insulin resistance (IR), and NAFLD with areas of necroinflammation. These conditions are associated with a poor prognosis.
Maternal Vitamin D Deficiency Delays Glomerular Maturity in F1 and F2 Offspring
Fernanda A. M. Nascimento, Thais C. Ceciliano, Marcia B. Aguila, Carlos A. Mandarim-de-Lacerda
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041740
Abstract: Background There is a high prevalence of vitamin D insufficiency in women of reproductive age. Methods This work studied the first two generations of offspring (F1 and F2) of Swiss mice from mothers fed one of two diets: SC (standard chow) or VitD- (vitamin D-deficient). Functional and developmental kidney measurements were taken. Results The first two generations of the VitD- group had higher blood pressure at 6 months of age than the offspring of the SC group as well as an increase in renin and AT1r expression. However, at all ages, both F1 and F2 VitD- mice had shorter glomerular diameters, and diet played a significant role in the total variation. Both the F1 and F2 generations of the VitD- group had more immature glomeruli than offspring from the SC group. Immature glomeruli begin to disappear at 10 days, but at this age, F1-VitD- mice had more immature and mature glomeruli than F1-SC mice. At 6 months of age, F1-VitD- mice exhibited more glomeruli, while F2-VitD- mice exhibited the same number of glomeruli as F2-SC mice, but fewer glomeruli compared to the F1-VitD group. Both diet and generation account for the total variation in the number of glomeruli. Decreases in urine output and podocin expression and increases in urea and creatinine in the urine were observed in F1 offspring. Conclusion These findings demonstrate that maternal vitamin D deficiency accompanies changes in the renal expression of important factors that may retard the maturation of glomeruli by extending the period of nephrogenesis.
Weight Cycling Enhances Adipose Tissue Inflammatory Responses in Male Mice
Sandra Barbosa-da-Silva, Julio C. Fraulob-Aquino, Jessica R. Lopes, Carlos A. Mandarim-de-Lacerda, Marcia B. Aguila
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0039837
Abstract: Background Obesity is associated with low-grade chronic inflammation attributed to dysregulated production, release of cytokines and adipokines and to dysregulated glucose-insulin homeostasis and dyslipidemia. Nutritional interventions such as dieting are often accompanied by repeated bouts of weight loss and regain, a phenomenon known as weight cycling (WC). Methods In this work we studied the effects of WC on the feed efficiency, blood lipids, carbohydrate metabolism, adiposity and inflammatory markers in C57BL/6 male mice that WC two or three consecutive times by alternation of a high-fat (HF) diet with standard chow (SC). Results The body mass (BM) grew up in each cycle of HF feeding, and decreased after each cycle of SC feeding. The alterations observed in the animals feeding HF diet in the oral glucose tolerance test, in blood lipids, and in serum and adipose tissue expression of adipokines were not recuperated after WC. Moreover, the longer the HF feeding was (two, four and six months), more severe the adiposity was. After three consecutive WC, less marked was the BM reduction during SC feeding, while more severe was the BM increase during HF feeding. Conclusion In conclusion, the results of the present study showed that both the HF diet and WC are relevant to BM evolution and fat pad remodeling in mice, with repercussion in blood lipids, homeostasis of glucose-insulin and adipokine levels. The simple reduction of the BM during a WC is not able to recover the high levels of adipokines in the serum and adipose tissue as well as the pro-inflammatory cytokines enhanced during a cycle of HF diet. These findings are significant because a milieu with altered adipokines in association with WC potentially aggravates the chronic inflammation attributed to dysregulated production and release of adipokines in mice.
CAN DIFFERENT LIPIDS DIETETICS AFFECT THE MYOCARDIAL STRUCTURE AND THE BLOOD PRESSURE INFLUENCING THE AGING PROCESS? EXPERIMENTAL STUDY IN RATS
Aguila,Marcia Barbosa; Mandarim-de-Lacerda,Carlos Alberto;
Revista chilena de anatomía , 2001, DOI: 10.4067/S0716-98682001000300014
Abstract: we studied sixty-seven male rats (wistar strain) from birth until three different ages: 6-mo-old, 12-mo-old and 18-mo-old. the animals were separated according to the different experimental diets, i.e., soybean oil (s), canola oil (ca), lard and egg yolk (le) and canola oil+lard and egg yolk (ca+le). the systolic blood pressure (sbp) was verified weekly. the determination of the numerical density of the left ventricular myocyte nuclei (nv[cm]) used the "optical disector" method. considering all ages, the ca group rats had the nv[cm] higher since 6-mo-old, whereas the other groups had almost the some values. the 18-mo-old, the ca group rats had a slight decrease of nv[cm] but smaller than the other groups. the 18-mo-old rats had higher sbp and lower nv[cm] than the younger ones, but in the ca group the sbp did not alter with the age and the reduction of the nv[cm] was significantly lower than that of the other groups. therefore, the variation in the sbp and the nv[cm] was age-dependent but each experimental group had a different tendency. the elevation of the sbp and the reduction of the nv[cm] was marked in the le group, and intermediary in the other groups, suggesting that the density of the cardiac myocytes was influenced by the different dietetic lipids. in conclusion, the present study verified that the edible oil type in diet concerns the blood pressure and the numerical density of cardiac myocytes in rats during the aging process. the long-term canola oil diet produces beneficial structural myocardial changes and the attenuation of the blood pressure than other experimental diets, mainly the diet composing of lard and egg yolk
CAN DIFFERENT LIPIDS DIETETICS AFFECT THE MYOCARDIAL STRUCTURE AND THE BLOOD PRESSURE INFLUENCING THE AGING PROCESS? EXPERIMENTAL STUDY IN RATS PUEDEN DIFERENTES LíPIDOS DIETéTICOS AFECTAR LA ESTRUCTURA DEL MIOCARDIO Y LA PRESIóN SANGUíNEA, INFLUENCIANDO EL PROCESO DE ENVEJECIMIENTO? ESTUDIO EXPERIMENTAL EN RATAS
Marcia Barbosa Aguila,Carlos Alberto Mandarim-de-Lacerda
Revista chilena de anatomía , 2001,
Abstract: We studied sixty-seven male rats (Wistar strain) from birth until three different ages: 6-mo-old, 12-mo-old and 18-mo-old. The animals were separated according to the different experimental diets, i.e., soybean oil (S), canola oil (CA), lard and egg yolk (LE) and canola oil+lard and egg yolk (CA+LE). The systolic blood pressure (SBP) was verified weekly. The determination of the numerical density of the left ventricular myocyte nuclei (Nv[cm]) used the "optical disector" method. Considering all ages, the CA group rats had the Nv[cm] higher since 6-mo-old, whereas the other groups had almost the some values. The 18-mo-old, the CA group rats had a slight decrease of Nv[cm] but smaller than the other groups. The 18-mo-old rats had higher SBP and lower Nv[cm] than the younger ones, but in the CA group the SBP did not alter with the age and the reduction of the Nv[cm] was significantly lower than that of the other groups. Therefore, the variation in the SBP and the Nv[cm] was age-dependent but each experimental group had a different tendency. The elevation of the SBP and the reduction of the Nv[cm] was marked in the LE group, and intermediary in the other groups, suggesting that the density of the cardiac myocytes was influenced by the different dietetic lipids. In conclusion, the present study verified that the edible oil type in diet concerns the blood pressure and the numerical density of cardiac myocytes in rats during the aging process. The long-term canola oil diet produces beneficial structural myocardial changes and the attenuation of the blood pressure than other experimental diets, mainly the diet composing of lard and egg yolk Estudiamos 67 ratas machos (Wistar) desde el nacimiento hasta: 6, 12 y 18 meses. Los animales fueron separados de acuerdo a las diferentes dietas experimentales: aceite de soya, aceite de canola (AC), manteca de cerdo y yema de huevo (CH) y aceite de canola + manteca de cerdo y yema de huevo (AC+CH). La presión arterial (PA) fue medida semanalmente. Para determinar la densidad numérica de núcleos de miocitos del ventrículo izquierdo (Nv[cm]), se utilizó el método del disector óptico. Considerando las edades de las ratas, las del grupo CA tuvieron el Nv[cm] mayor desde los 6 meses de edad, mientras que los otros grupos tuvieron, prácticamente, los mismos valores. A los 18 meses, el grupo de ratas AC tuvo una peque a disminución del Nv[cm], menor que en los otros grupos. Las ratas de 18 meses presentaron los mayores valores de PA y menores valores de Nv[cm] en comparación con las ratas más jóvenes. En el grupo AC, la PA no se
Enterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from Galicia, Spain
Lores, B;Aguila, C del;Arias, C;
Memórias do Instituto Oswaldo Cruz , 2002, DOI: 10.1590/S0074-02762002000700003
Abstract: in this survey we examined 87 domestic animal stool samples in order to detect the possible presence of microsporidia in animals in close contact with humans in galicia (nw, spain). the detection of enterocytozoon bieneusi spores was confirmed in faecal samples from two dogs and one goat by polymerase chain reaction. none of the positive samples for microsporidia in the staining method were amplified with species-specific primers for encephalitozoon intestinalis, e. hellem and e. cuniculi. four rabbits faecal samples reacted with anti-e. cuniculi serum. our results could indicate the importance of domestic animals as zoonotic reservoirs of microsporidial human infections.
Enterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from Galicia, Spain
Lores B,Aguila C del,Arias C
Memórias do Instituto Oswaldo Cruz , 2002,
Abstract: In this survey we examined 87 domestic animal stool samples in order to detect the possible presence of microsporidia in animals in close contact with humans in Galicia (NW, Spain). The detection of Enterocytozoon bieneusi spores was confirmed in faecal samples from two dogs and one goat by polymerase chain reaction. None of the positive samples for microsporidia in the staining method were amplified with species-specific primers for Encephalitozoon intestinalis, E. hellem and E. cuniculi. Four rabbits faecal samples reacted with anti-E. cuniculi serum. Our results could indicate the importance of domestic animals as zoonotic reservoirs of microsporidial human infections.
Z' Decays into Four Fermions
F. del Aguila,B. Alles,Ll. Ametller,A. Grau
Physics , 1992, DOI: 10.1103/PhysRevD.48.425
Abstract: If a new $Z'$ is discovered with a mass $\sim 1 \ TeV$ at LHC/SSC, its (rare) decays into two charged leptons plus missing transverse energy will probe the $Z'$ coupling to the lepton doublet $(\nu,e)_L$ and to $W^+W^-$, allowing further discrimination among extended electroweak models.
Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet Alteraciones Somáticas Bioquímicas y hepáticas en un Tipo de Ratas Alimentadas Crónicamente con Dieta Alta en Grasas
Renata Heisler Neves,Alba Cristina Miranda de Barros Alencar,Marcia Barbosa Aguila,Carlos Alberto Mandarim-de-Lacerda
International Journal of Morphology , 2006,
Abstract: This study evaluated whether a high fat diet (HFC group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (SC group). Female mice were submitted to each diet over 6 months. Body mass and food intake were evaluated weekly throughout the experiment. Total cholesterol, TG, LDL-c, HDL-c and VLDL-c were analyzed. Mice were sacrificed to remove liver, spleen, heart and intestine. The volume of the organs was determined according to the submersion method. Fixed livers were embedded in paraffin and stained with hematoxylin and eosin and Masson's trichrome. The analysis used a video microscope system and a test-system with 42 test-points. The volume density was estimated for hepatocytes, steatosis and sinusoids. Animals fed HFC had smaller chow intake than SC group. HFC group presented body mass greater than SC. Animals fed HFC showed heavier liver and spleen and lighter intestine than SC (p<0.05), heart mass was not significant between groups. Plasma lipoproteins differed between groups (p<0.05) except VLDL-c and TG fractions. The liver structure was without major alteration in SC group however, HFC mice group showed different degrees of fatty degeneration with micro- and macrovesicular steatosis dispersed in all liver with typical peri-cellular/peri-sinusoidal fibrosis. The quantitative study showed significant (p<0.05) volume density reduction for hepatocytes and sinusoids. In conclusion, our results clearly show that hepatic steatosis can be induced in mouse by such a fat-rich diet without any toxin ingestion, alimentary deficiency and genes depletion Este estudio evaluó cómo una dieta de alta densidad energética (grupo ADE) induce sobrepeso, esteatosis hepática y altera los niveles de las lipoproteínas plasmáticas cuando son comparados con la dieta patrón (grupo SC). Hembras de camundongos fueron sometidas a cada una de las dietas durante 6 meses. La masa corporal y la ingestión de alimento fueron evaluadas semanalmente durante el experimento. Además fueron medidos el colesterol total, TG, LDL-c, HDL-c e VLDL-c. Los animales fueron sacrificados y el hígado, bazo, corazón e intestinos fueron removidos para estudio. El volumen de los órganos fue medido por el método de la sumersión. Fragmentos de hígado fueron preparados para el estudio en microscopía de luz, te idos con hematoxilina-eosina y tricrómico de Masson. El análisis fue realizado con video microscopía y sistema test M42. La densidad de volumen fue estimada para hepatocitos, esteatosis y sinusoides. Los animales alimentados con dieta ADE pre
Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
Neves,Renata Heisler; Barros Alencar,Alba Cristina Miranda de; Aguila,Marcia Barbosa; Mandarim-de-Lacerda,Carlos Alberto; Machado-Silva,José Roberto; Gomes,Delir Corrêa;
International Journal of Morphology , 2006, DOI: 10.4067/S0717-95022006000500018
Abstract: this study evaluated whether a high fat diet (hfc group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (sc group). female mice were submitted to each diet over 6 months. body mass and food intake were evaluated weekly throughout the experiment. total cholesterol, tg, ldl-c, hdl-c and vldl-c were analyzed. mice were sacrificed to remove liver, spleen, heart and intestine. the volume of the organs was determined according to the submersion method. fixed livers were embedded in paraffin and stained with hematoxylin and eosin and masson's trichrome. the analysis used a video microscope system and a test-system with 42 test-points. the volume density was estimated for hepatocytes, steatosis and sinusoids. animals fed hfc had smaller chow intake than sc group. hfc group presented body mass greater than sc. animals fed hfc showed heavier liver and spleen and lighter intestine than sc (p<0.05), heart mass was not significant between groups. plasma lipoproteins differed between groups (p<0.05) except vldl-c and tg fractions. the liver structure was without major alteration in sc group however, hfc mice group showed different degrees of fatty degeneration with micro- and macrovesicular steatosis dispersed in all liver with typical peri-cellular/peri-sinusoidal fibrosis. the quantitative study showed significant (p<0.05) volume density reduction for hepatocytes and sinusoids. in conclusion, our results clearly show that hepatic steatosis can be induced in mouse by such a fat-rich diet without any toxin ingestion, alimentary deficiency and genes depletion
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