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Search Results: 1 - 10 of 150768 matches for " Marcelo Sim?o;Costa-Cruz "
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Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis
Barcelos, Ivanildes Solange da Costa;Moura, Leandro Pajuaba de;Costa, Vinicius Paulino da;Ferreira, Marcelo Simo;Costa-Cruz, Julia Maria;
Memórias do Instituto Oswaldo Cruz , 2007, DOI: 10.1590/S0074-02762007005000084
Abstract: the aim of this study was to test if serological distinction between patients with active and inactive neurocysticercosis (ncc), could be accomplished by the recognition of immunodominant peptides in total saline antigenic extract of taenia solium metacestodes by igg antibody in cerebrospinal fluid (csf) and serum paired samples. csf and serum samples of 10 each, active ncc patients, inactive ncc, and individuals with other neurological disorders, were used to recognize the antigenic peptides by western blot (wb). in the active ncc the 28-32 and 39-42 kda peptides were more frequently detected in csf than in sera (p < 0.05). the 47-52, 64-68, and 70 kda antigens showed high frequencies in both samples from patients with active ncc. all the csf samples of inactive ncc and other neurological disorder (control) patients tested negative, while serum samples from these last two groups recognized mainly the 80, 86, 95, and 98 kda bands. this finding eliminates the use of the high molecular weigh bands (> 80 kda) for diagnosis of ncc. the final conclusions were that the difference between active and inactive ncc may be done with the detection of peptides only in the csf samples and that the 47-52, 64-68, and 70 kda bands may be included as specific markers for active ncc when detected in csf samples by wb using total saline extract of t. solium metacestode.
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis
Barcelos, Ivanildes Solange da Costa;Ferreira, Marcelo Simo;Moura, Leandro Pajuaba de;Biondi, Germano Francisco;Costa-Cruz, Julia Maria;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000400014
Abstract: paired samples of cerebrospinal fluid (csf) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (ncc), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (elisa) using two taenia crassiceps metacestode extracts as antigen in order to detect igg antibodies. in active ncc, high levels of igg were detected (p < 0.05). the csf samples showed 80% (ci 72-88) of reactivity in the saline extract (s) and 90% (ci 84-95) in sodium dodecyl sulphate (sds) and the serum samples were reactive in 90% (ci 84-95) and 100% (ci 98-100) in the s and sds antigenic extracts, respectively. the use of the paired samples of csf and serum in active ncc showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose csf puncture is counter-indicated.
Detection of Cryptosporidium - specific coproantigen in human immunodeficiency virus/acquired immunodeficiency syndrome patients by using a commercially available immunoenzymatic assay
Silva, Claudio Vieira;Ferreira, Marcelo Simo;Gon?alves-Pires, Maria do Rosário de Fátima;Costa-Cruz, Julia Maria;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000800022
Abstract: the aim of this study was to verify the occurrence of cryptosporidium infection in 52 human immunodeficiency virus (hiv)/acquired immunodeficiency syndrome (aids) patients (group 1) and 38 clinically healthy individuals (group 2) by using enzyme immunoassay (eia). all fecal samples collected were submitted to the baermann, lutz, and ritchie methods, the safranin/methylene blue, and weber's chromotrope modified trichrome staining techniques, and eia. in group 1, parasitological staining techniques and eia were both positive for cryptosporidium sp. infection in 3/52 (5.8%) samples and both negative in 45/52 (86.5%) samples, while 4/52 (7.7%) samples were positive in eia and negative in parasitological staining techniques. concerning group 2, all samples were negative by eia and microscopy for cryptosporidium infection. in conclusion, eia may be an alternative method for detecting cryptosporidium-specific coproantigen in hiv/aids patients.
Detection of Cryptosporidium - specific coproantigen in human immunodeficiency virus/acquired immunodeficiency syndrome patients by using a commercially available immunoenzymatic assay
Silva Claudio Vieira,Ferreira Marcelo Simo,Gon?alves-Pires Maria do Rosário de Fátima,Costa-Cruz Julia Maria
Memórias do Instituto Oswaldo Cruz , 2003,
Abstract: The aim of this study was to verify the occurrence of Cryptosporidium infection in 52 human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients (group 1) and 38 clinically healthy individuals (group 2) by using enzyme immunoassay (EIA). All fecal samples collected were submitted to the Baermann, Lutz, and Ritchie methods, the Safranin/Methylene Blue, and Weber's chromotrope modified Trichrome staining techniques, and EIA. In group 1, parasitological staining techniques and EIA were both positive for Cryptosporidium sp. infection in 3/52 (5.8%) samples and both negative in 45/52 (86.5%) samples, while 4/52 (7.7%) samples were positive in EIA and negative in parasitological staining techniques. Concerning group 2, all samples were negative by EIA and microscopy for Cryptosporidium infection. In conclusion, EIA may be an alternative method for detecting Cryptosporidium-specific coproantigen in HIV/AIDS patients.
Intestinal parasites in school food handlers in the city of Uberlandia, Minas Gerais, Brazil
Costa-Cruz, Julia Maria;Cardoso, Margareth Leit?o Gennari;Marques, Daldy Endo;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1995, DOI: 10.1590/S0036-46651995000300002
Abstract: in order to verify the presence of intestinal parasites in food handlers, stool samples were collected from 104 cooks and their helpers that were working in food preparation in 20 public elementary schools, in various areas of the city of uberlandia, minas gerais, brazil. the samples were collected during the months of november and december, 1988, in plastic flasks containing a 10% formaldehyde solution and processed by the hoffmann, pons & janer method. the sediment was examined using triplicate slides. all individuals were females aged between 24 to 69 years. intestinal parasites were found in 85.0% of the studied schools and 47.1% of the studied food handlers (cooks and helpers) were found to be positive. among the 49 infected food handlers, 32 (65.3%) carried a single parasite and 17 (34.7%) carried two parasites. the following intestinal parasites were found: giardia lamblia (21.1%), entamoeba coli (21.1%), hookworms (9.6%), ascaris lumbricoides (5.8%), entamoeba histolytica (2.9%), hymenolepis nana (1.9%), strongyloides stercoralis (1.0%). these data emphasize the need for a rigid semi-annual control in all school food handlers, including diagnosis, specific treatment and orientation about the mechanisms of transmission of the intestinal parasites.
Ocorrência de parasitas e comensais intestinais em crian?as de escola localizada em assentamento de sem-terras em Campo Florido, Minas Gerais, Brasil
Ferreira, Patricia;Lima, Marcelo Ribeiro;Oliveira, Francielle Batista;Pereira, Maria Letícia Moreira;Ramos, Leila Bitar Moukachar;Mar?al, Maria das Gra?as;Costa-Cruz, Julia Maria;
Revista da Sociedade Brasileira de Medicina Tropical , 2003, DOI: 10.1590/S0037-86822003000100015
Abstract: the aim of this study was to verify the occurrence of intestinal parasites and commensal organisms among children attending a school located in a settlement of 'landless farm workers' in campo florido, minas gerais, brazil. coproparasitological analyses performed on 72 school children revealed 59.7% positivity and 4 types of protozoa and 5 types of helminths were identified. it can be concluded that it is necessary to monitor the health conditions of this population.
Cryptosporidium sp. in children suffering from acute diarrhea at Uberlandia City, State of Minas Gerais, Brazil
Gennari-Cardoso, Margareth Leit?o;Costa-Cruz, Julia Maria;Castro, Eliseo de;Lima, Leda Maria FS;Prudente, Divino V;
Memórias do Instituto Oswaldo Cruz , 1996, DOI: 10.1590/S0074-02761996000500003
Abstract: this study's objective was to search for cryptosporidium sp. in diarrheic feces from children aged zero to 12 years and cared for at medical units within universidade federal de uberlandia or at a private practice in uberlandia, state of minas gerais, brazil, from september 1992 to august 1993. three fecal samples preserved in 10% formalin, were collected from 94 children. oocyst concentration was performed through ritchie's (modified) method and staining of fecal smears for each sample (total of 1128 slides) was done by the "safranin/methylene blue" and the "kinyoun (modified)" techniques. the hoffmann, pons & janer method was also employed to look for other enteroparasites. from 94 children, 4.26% excreted fecal cryptosporidium oocysts. the infection seemed to vary according to age: 5.08% of patients aged zero to two years old; 33.33% of those aging eight to ten years (p>0.05). cryptosporidium appeared in november, december and march, during the rainy season. 20.21% of the children harbored at least one enteroparasite different from cryptosporidium, mainly giardia intestinalis (12.77%). from cryptosporidium infected patients, two had only this kind, another harbored giardia intestinalis; the last one hosted strongyloides stercoralis.
Ocorrência de cisticercose em necropsias realizadas em Uberlandia, Minas Gerais, Brasil
Costa-Cruz, Julia Maria;Rocha, Ademir;Silva, Arnaldo M.;Moraes, Azarias T. de;Guimar?es, Aparecida H. B.;Salom?o, Eliana C.;Alcantara, Tania M.;
Arquivos de Neuro-Psiquiatria , 1995, DOI: 10.1590/S0004-282X1995000200007
Abstract: 3937 autopsies were performed between 1971 and 1993 in the servi?o de anatomia patológica of the hospital de clínicas of the funda??o de assistência, estudo e pesquisa de uberlandia, universidade federal de uberlandia, in minas gerais, brazil. at this service of pathology are realized all the autopsies of the municipal district of uberlandia. the analysis of 2862 concluded autopsy reports, of death above the age of one year, disclosed 39 cases (1.4%) of cysticercosis. the age range was 16 to 83 years and 66.6% were males; 82.1 % of the patients were from minas gerais state, 15.4% were from goiás state, and in one case (2.5%) the origin was not registered. from these 39 cases, 35 (89.7%) showed central nervous system involvement, isolated or in association to other clinical forms of the disease; in 9 occurred the isolated or associated cardiac form; in 4 the muscular form, isolated or associated, was found; 4 presented the isolated or associated visceral form. in only 7 (17.9%) cases, the cysticercosis was assumed to be the direct cause of the death.
Hydrophobic fraction of Taenia saginata metacestodes, rather than hydrophilic fraction, contains immunodominant markers for diagnosing human neurocysticercosis
Gon?alves, Flávia de Assun??o;Machado, Gleyce Alves;Oliveira, Heliana Batista;Rezende, Maria Teresa Nunes Pacheco;Mineo, José Roberto;Costa-Cruz, Julia Maria;
Revista da Sociedade Brasileira de Medicina Tropical , 2010, DOI: 10.1590/S0037-86822010000300008
Abstract: introduction: considering that alternative antigens for diagnosing neurocysticercosis continue to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for preparation of taenia solium homologous antigen, the aim of the present study was to evaluate the detergent (d) and aqueous (a) fractions from saline extract of taenia saginata metacestodes for diagnosing neurocysticercosis. methods: taenia saginata was obtained from naturally infected bovines in the triangulo mineiro region, state of minas gerais, brazil. the carcasses came from cold storage units and had been slaughtered in accordance with the inspection technique recommended by the federal inspection service. the d and a fractions were obtained by using triton x-114 (tx-114). serum samples were obtained from 40 patients with a diagnosis of neurocysticercosis, 45 with other parasitic diseases and 30 from apparently normal individuals. igg antibody levels were evaluated using the elisa and immunoblotting assays. results: the elisa sensitivity and specificity were 95% and 73.3%, when using saline extract; 95% and 82.6% for the d fraction; and 65% and 61.3% for the a fraction, respectively. the immunoblotting assay confirmed the elisa results, such that the d fraction was more efficient than the other extracts, and the 70-68kda component was immunodominant among neurocysticercosis patients. conclusions: these results demonstrated that the d fraction from taenia saginata metacestodes obtained using tx-114 can be used as a heterologous antigenic fraction in the immunoblotting assay for serologically diagnosing human neurocysticercosis, given its ability to select immunodominant antigens.
Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay
Machado, Eleuza Rodrigues;Ueta, Marlene Tiduko;Gon?alves-Pires, Maria do Rosário de Fátima;Oliveira, Jo?o Batista Alves de;Faccioli, Lúcia Helena;Costa-Cruz, Julia Maria;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000600024
Abstract: the present study was conducted to detected igg antibodies using strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (elisa). sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). results were expressed in antibody titers, which were considered as positive when titer was > 80. sensibility and specificity of the assay were 100% and 96.7%, respectively. it can be concluded that the heterologous alkaline extract could be employed in elisa as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.
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