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Search Results: 1 - 10 of 43841 matches for " Marcelo Luiz;Martins "
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Chromosome spreading of associated transposable elements and ribosomal DNA in the fish Erythrinus erythrinus. Implications for genome change and karyoevolution in fish
Marcelo B Cioffi, Cesar Martins, Luiz AC Bertollo
BMC Evolutionary Biology , 2010, DOI: 10.1186/1471-2148-10-271
Abstract: Conspicuous differences in the distribution of the 5S rDNA and Rex3 non-LTR retrotransposon were found between the two karyomorphs, while no changes in the heterochromatin and 18S rDNA patterns were found between them. Rex3 was interstitially dispersed in most chromosomes. It had a compartmentalized distribution in the centromeric regions of only two acrocentric chromosomes in karyomorph A. In comparison, in karyomorph D, Rex3 was found in 22 acrocentric chromosomes in females and 21 in males. All 5S rDNA sites co-localized with Rex3, suggesting that these are associated in the genome. In addition, the origin of the large metacentric Y chromosome in karyomorph D by centric fusion was highlighted by the presence of internal telomeric sites and 5S rDNA/Rex3 sites on this chromosome.We demonstrated that some repetitive DNAs (5S rDNA, Rex3 retroelement and (TTAGGG)n telomeric repeats) were crucial for the evolutionary divergence inside E. erythrinus. These elements were strongly associated with the karyomorphic evolution of this species. Our results indicate that chromosomal rearrangements and genomic modifications were significant events during the course of evolution of this fish. We detected centric fusions that were associated with the differentiation of the multiple sex chromosomes in karyomorph D, as well as a surprising increase of associated 5S rDNA/Rex3 loci, in contrast to karyomorph A. In this sense, E. erythrinus emerges as an excellent model system for better understanding the evolutionary mechanisms underlying the huge genome diversity in fish. This organism can also contribute to understanding vertebrate genome evolution as a whole.Repetitive DNA sequences include tandemly-arrayed satellites, as well as minisatellites, microsatellites and dispersed repeats such as transposable elements (TEs) [1]. Satellite DNAs are organized as long arrays of head-to-tail linked repeats. TEs are DNA segments capable of integrating into new locations in the genome, and the
Comparative chromosome mapping of repetitive sequences. Implications for genomic evolution in the fish, Hoplias malabaricus
Marcelo B Cioffi, Cesar Martins, Luiz AC Bertollo
BMC Genetics , 2009, DOI: 10.1186/1471-2156-10-34
Abstract: The results showed that several chromosomal markers had conserved location in the four karyomorphs. In addition, some other markers were only conserved in corresponding chromosomes of karyomorphs A-B and C-D. These data therefore reinforced and confirmed the proposed grouping of karyomorphs A-D in Group I and highlight a closer relationship between karyomorphs A-B and C-D. Moreover, the mapping pattern of some markers on some autosomes and on the chromosomes of the XY and X1X2Y systems provided new evidence concerning the possible origin of the sex chromosomes.The in situ investigation of repetitive DNA sequences adds new informative characters useful in comparative genomics at chromosomal level and provides insights into the evolutionary relationships among Hoplias malabaricus karyomorphs.Although usually reported as a single taxonomic entity, Hoplias malabaricus (Characiformes, Erythrinidae) has significant karyotypic diversity and well-defined population differences concerning the diploid number, morphology of chromosomes and sex chromosome systems. Such intraspecific diversity enabled the characterization of seven main karyomorphs (A-G), in which those without differentiated sex chromosome systems (A, C, E and F) show a wider geographical distribution compared to those that have such systems (B, D and G), which highlights the derivative character of the latter [1]. Despite differences in diploid chromosome number and in the presence or absence of differentiated sex chromosome systems, the seven karyomorphs were subdivided into two major groups (I and II) based on general karyotype similarity [1]. Thus, Group I included karyomorphs A-D, while Group II included karyomorphs E-G (Table 1).The karyotype diversity in H. malabaricus indicates the probable occurrence of distinct species, which is reinforced by the sympatry between some karyomorphs, without evidence of gene flow between them [1]. Specifically for karyomorphs A and C, and for karyomorphs A and D, the resu
Dezessete anos de judicializa??o da política
Vianna, Luiz Werneck;Burgos, Marcelo Baumann;Salles, Paula Martins;
Tempo Social , 2007, DOI: 10.1590/S0103-20702007000200002
Abstract: this article studies the judicialization of politics in brazil, based on an analysis of a database containing 3.648 adins filed between 1988 and 2005. examining this data, the overall impression gained is that adins are now a normal part of the everyday running of the modern brazilian democracy, their institutional presence consolidated over almost two decades through successive and different governments - and even more firmly in the current lula government. over this time, they have functioned as a channel for the conflicts between society and the state, as well as conflicts within the public administration and the federation. hence, as well as being an instrument for the defence of minorities, their original constitutional function, the research shows that the adins are also a strategic institutional resource for government, turning the federal supreme court in practice into a kind of council of state prevalent in countries with unitary governments.
Varia??o química do óleo essencial de Hyptis suaveolens (L.) Poit., sob condi??es de cultivo
Martins, Felipe Terra;Santos, Marcelo Henrique dos;Polo, Marcelo;Barbosa, Luiz Cláudio de Almeida;
Química Nova , 2006, DOI: 10.1590/S0100-40422006000600011
Abstract: this study was performed to establish the correlation between the growth conditions and essential oil composition of hyptis suaveolens from alfenas (mg), brazil. the plants were grown in a greenhouse, four treatments were used and they were harvested at two different periods of time (60 and 135-day-old plants). the essential oil composition was determined by gc-ms analysis. the terpenes spathulenol, globulol, dehydroabietol, a-cadinol and b-phellandrene were the major constituents found in the essential oil. oxygenated sesquiterpenes represented the main group of constituents in most of the treatments. the major changes in the essential oil composition were found in 135-day-old plants grown under npk deficiency. we also identified three groups of volatile components that have not been previously described in h suaveolens.
Valor prognóstico do teste de caminhada de seis minutos na insuficiência cardíaca
Rubim, Valéria Siqueira Martins;Drumond Neto, Cantídio;Romeo, José Luiz Martins;Montera, Marcelo Westerlund;
Arquivos Brasileiros de Cardiologia , 2006, DOI: 10.1590/S0066-782X2006000200007
Abstract: objective: this study aimed to assess the true usefulness of the six-minute walk test as a prognostic indicator and its contribution to clinical practice with heart failure patients. methods: in order to investigate the actual value of the six-minute walk test as an objective measure of mortality probability in patients with heart failure, the test was applied to 179 stable patients (120 men and 59 women, mean age 58.32 ± 12.7 years, with nyha class ii and iii heart failure and an ejection fraction (lvef) of 34.91 ± 12.4%). patients were instructed to walk for 6 minutes and then, four hours later, underwent a conventional exercise stress test (as per naughton protocol). patients were followed for an average of eighteen months. results: the average distance walked was 521.11 ± 76.1 meters. during the follow-up period, 66 patients (36.9%) died. there was a significant correlation between the distance walked during the test and mortality (p < 0.0001). the logistic regression model identified the distance walked during the test as the most important independent predictor of mortality (p = 0.0001). a distance shorter than 520 meters identified the patients with an increased probability of death. there was a significant correlation between the number of metabolic equivalents (mets) measured during the conventional exercise stress testing and mortality rate (p = 0.0001). conclusion: the six-minute walk test is a simple, safe and powerful method to assess the prognosis of patients with nyha class ii and iii heart failure. it is an objective examination that may replace the conventional ergometric test for the prognostic evaluation of these patients.
Valor prognóstico do teste de caminhada de seis minutos na insuficiência cardíaca
Rubim Valéria Siqueira Martins,Drumond Neto Cantídio,Romeo José Luiz Martins,Montera Marcelo Westerlund
Arquivos Brasileiros de Cardiologia , 2006,
Abstract: OBJETIVOS: Este estudo objetivou avaliar a utilidade do teste de caminhada de seis minutos como indicador prognóstico e sua contribui o na prática clínica diária de pacientes com insuficiência cardíaca. MéTODOS: O teste de caminhada de seis minutos foi administrado a 179 pacientes (120 homens, 59 mulheres; idade média de 58,32 ± 12,7 anos), portadores insuficiência cardíaca nas classes II e III da New York Heart Association (NYHA), com fra o de eje o do ventrículo esquerdo de 34,91 ± 12,4%. Os pacientes realizaram o teste de caminhada de seis minutos e, 4 horas depois, o teste ergométrico convencional sob o Protocolo de Naughton limitado por sintomas, e foram acompanhados por um período médio de dezoito meses. RESULTADOS: A distancia média percorrida no teste de caminhada seis minutos foi de 521,11 ± 76,1 metros. Durante o acompanhamento, 66 pacientes (36,9%) morreram. Houve uma correla o significativa entre a distancia percorrida no teste de caminhada de seis minutos e a mortalidade (p < 0,0001). O modelo de regress o logística identificou a distancia percorrida durante o teste de caminhada de seis minutos como o mais forte indicador independente de mortalidade (p = 0,0001). A distancia caminhada menor que 520 metros identificou os pacientes com maior probabilidade de óbito. O número de equivalentes metabólicos alcan ados no teste ergométrico convencional também correlacionou-se significativamente com a mortalidade (p = 0,0001). CONCLUS O: O teste de caminhada de seis minutos é um método simples, seguro e potente de avalia o prognóstica de portadores de insuficiência cardíaca nas classes II e III da NYHA. é um exame objetivo, que pode substituir o teste ergométrico convencional na avalia o prognóstica desses pacientes.
Patellofemoral instability: evaluation by magnetic resonance imaging Instabilidade femoropatelar: avalia o por ressonancia magnética
Patrícia Martins e Souza,Marcelo Sadock de Sá Gabetto,Marcelo Ganem Serr?o,Luiz Ant?nio Martins Vieira
Revista Brasileira de Ortopedia , 2013,
Abstract: OBJECTIVE: To determine the contribution of magnetic resonance imaging (MRI) in evaluating patelofemoral instability. METHODS: 39 patients (45 knees) with patellar instability underwent knee magnetic resonance images between October 2009 and July 2011, at the Rede Lab's D'Or, in the city of Rio de Janeiro, State of Rio de Janeiro, were included. MRI were analyzed for the presence of bone, hyaline cartilage and soft-tissue abnormalities, as well as anatomic variants that may contribute to chronic patellar instability. RESULTS: The authors found, of the 45 knees analysed, bone changes in 44%, cartilaginous injuries in 64%, disruption of the medial patellofemoral ligament (MPFL) in 29% and joint morphology abnormalities in 73% patients. Meniscal tears were also identified in 2 (4%) patients and fibular fracture in one (2%) patient. CONCLUSION: MRI allowed the detection of predisposing factors that may contribute to the development of patellofemoral instability and the diagnosis of bone, hyaline cartilage, ligamentous and meniscal abnormalities. OBJETIVO: Determinar a contribui o da ressonancia magnética (RM) na avalia o da instabilidade femoropatelar. MéTODOS: Foram incluídos 39 pacientes (45 joelhos) com instabilidade femoropatelar, submetidos à RM do joelho entre outubro de 2009 e julho de 2011, na Rede Labs D'Or, Rio de Janeiro (RJ). Os resultados dos exames foram analisados quanto à presen a de altera es ósseas, cartilaginosas e nas partes moles, além de altera es anat micas que podem contribuir para a instabilidade patelar cr nica. RESULTADOS: Os autores encontraram, nos 45 joelhos examinados, altera es ósseas em 44%, les es da cartilagem em 64%, ruptura do ligamento patelofemoral medial (LPFM) em 29% e anormalidades da morfologia articular em 73% pacientes. Foram também identificadas les es meniscais em dois (4%) pacientes e fratura da fíbula em um (2%) paciente. CONCLUS O: A RM permitiu a detec o de fatores predisponentes à instabilidade e o diagnóstico de les es ósseas, cartilaginosas, meniscais e ligamentares.
Impact of coal mining on water quality of three artificial lakes in Morozini River Basin (Treviso, Santa Catarina State, Brazil)
Moschini-Carlos, Viviane;Pompêo, Marcelo Luiz Martins;Lobo, Felipe de Lucia;Meirelles, Sergio Tadeu;
Acta Limnologica Brasiliensia , 2011, DOI: 10.1590/S2179-975X2012005000007
Abstract: aim: to assess water quality of three lakes located in an abandoned coal mining area, in the morozini river basin (treviso, santa catarina state, brazil). methods: the study sites were three lakes mining pits flooded after deactivation, near the drainage basin of the morozini river (treviso city, santa catarina state, brazil). samplings were carried out in profile in the limnetic region during two seasons (summer in february and winter in july). results: in general, the three lakes showed high concentrations of so4-2, mg, fe, al, ca, ba, si, zn, ni, na, k, sr, b, cd, cr, pb and cu. conclusions: coal mining activity and waste disposal practices adopted by the mining companies in the morozini river basin have drastically affected the water quality of the examined lakes and made them inappropriate for diverse human uses, such as leisure, swimming and fishing.
Consumo de oxigênio e índice de esfor?o percebido em diferentes ritmos de execu??o na hidroginástica
Alberton, Cristine Lima;Rothman, Claudia Renata Cardoso;Pinto, Stephanie Santana;Coertjens, Marcelo;Kruel, Luiz Fernando Martins;
Motriz: Revista de Educa??o Física , 2012, DOI: 10.1590/S1980-65742012000300002
Abstract: the purpose of the study was to correlate the oxygen consumption (vo2) and the percentage of maximal vo2 (%vo2max) with the rate of perceived exertion (rpe) and to compare these variable between water aerobic exercises performed in aquatic environment at different rhythms. six women performed four test sessions, one on dry land environment (maximal effort test) and three in aquatic environment, each one with the performance of a water aerobic exercise: stationary running, jumping jacks and frontal kick up to 45°. each aquatic exercise was performed at three rhythms of execution, 80, 100 and 120 bpm, during 6 min (interval of 30 min). for all variables, no significant differences were observed between three exercises, however, significant differences were found between rhythms of execution for all of them, indicating that with the increase of the rhythm, the effort to perform the exercises was intensified. significant correlations were observed between vo2 and rpe, and between %vo2max and rpe. therefore, based on this association it is suggested that the prescription of intensity of the exercises analyzed can be done by rpe during water aerobics classes.
Proposi??o de um modelo crustal para a regi?o do Cone do Amazonas baseado em dados gravimétricos
Oliveira, Luiz Gabriel Souza de;Martins Neto, Marcelo Augusto;Barbosa, Maria Sílvia Carvalho;
Revista Brasileira de Geofísica , 2003, DOI: 10.1590/S0102-261X2003000100003
Abstract: the amazon cone, located in the brazilian equatorial margin, is constituted by a large deltaic sedimentation, which started in the middle miocene in response to the reversion of the amazon river drainage following the main andean orogenic stage. the crustal arrangement in the amazon cone region was investigated in this study, based on an integrated analysis of gravity data. crustal configuration showed by 2-d gravity modelling based on density models constructed with seismic interpretation results, allowing to verify: (1) sedimentary thickness; (2) crustal thickness and (3) morphology and depth of the crust-mantle interface. non-linear inversion algorithm was used, applying a density model with exponential variation (in terms of depth). thus, depths of the crust-mantle interface and crustal thickness values were adjusted, being coherent with refraction seismic data. the reached results in this work allowed the characterization of the crustal structure in the region, as well as the observation of the principal geological features.
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