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Search Results: 1 - 10 of 189107 matches for " Marcílio Hubner de;Lucas "
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Estudo micro-mesoscópico das granula??es aracnóides humanas
Miranda Neto, Marcílio Hubner de;Biazoto, Walter;Chopard, Renato Paulo;Lucas, Guilherme Araújo;
Arquivos de Neuro-Psiquiatria , 1990, DOI: 10.1590/S0004-282X1990000200001
Abstract: the functional anatomy of the arachnoid granulations of the upper sagittal sinus was studied by means of micromesoscopic techniques in order to contribute to elucidating aspects of drainage pathways of the cerebrospinal fluid through their fibrous components. the arrangement of fibrous elements was analysed at the base of the peduncle, peduncle, middle and apex of granulation. the analysis of seriate sections shows collagen bundles at the base of the peduncle with a predominantly circular morphology, longitudinally directed towards the peduncle's longest axis. these bundles arise in the middle of the granulation, them branch off towards its apex and periphery. the elastic bundles present an arrangement similar to that described for collagen bundles, delimiting with such bundles intercommunication channels from the base of the peduncle up to the apex of the granulation. bundles of reticular fibers arranged in between the collagen meshes were found at the base of the peduncle. the fibrous capsule of granulation is ccmposed of collagen bundles and a small number of elastic fibers.
Estudo da conectividade entre o nervo occipital maior e estruturas adjacentes considera??es anátomo-clínicas
Tanaka, Clarice;Biazotto, Walter;Chopard, Renato P.;Miranda Neto, Marcílio Hubner de;Lucas, Guilherme de Araújo;
Arquivos de Neuro-Psiquiatria , 1991, DOI: 10.1590/S0004-282X1991000100010
Abstract: in order to offer anatomical basis that aid for clinical interpretation of headache of cervical origin a macro-mesoscopic study of greater occipital nerve and its subcutaneous rise out site was accomplished. the authors observed that in its course this nerve delineates angles and direction shifts that can stand for critical points in etiology of occipital pain, so that in its subcutaneous rise out region both occipital artery and vein shape the vasculo-nervous bundle wrapped by sheath of fibrous connective tissue which has continuity and contiguity relation with the adjoining epimysium and perimysium. from our results, anatomo-clinical aspects are discussed.
MORPHOMETRY AND QUANTIFICATION OF THE MYENTERIC NEURONS OF THE DUODENUM OF ADULT RATS FED WITH HYPOPROTEIC CHOW
Mar?al Natali,Maria Raquel; Hubner de Miranda-Neto,Marcílio; Orsi,Ant?nio Marcos;
International Journal of Morphology , 2003, DOI: 10.4067/S0717-95022003000400003
Abstract: morphometric and quantitative analyses were carried out on the myenteric neurons of the duodenum, to study possible alterations resulting from a supply of low-protein level chow to adult wistar rats. the animals were divided in two groups: nourished (n) fed with ration containing 22% protein for 210 days and the undernourished (d) fed with ration containing 8% protein from the 90th day of age on and during the next 120 days. whole-mounts were stainedby the method of giemsa and the histochemical reaction of nadh-diaphorase enzyme, to estimate the neuronal population, which revealed a greater neuronal density in undernourished rats, regardless of the technique employed. the morphometric studies of the cell body profiles of 500 neurons of each group indicated a reduction on the cell body size and an increase on the proportion of small neurons on the rats subjected to the hypoproteic chow
Terminal ileum submucous plexus: Study of the VIP-ergic neurons of diabetic rats treated with ascorbic acid
Zanoni, Jacqueline Nelisis;Hernandes, Luzmarina;Bazotte, Roberto Barbosa;Miranda Neto, Marcílio Hubner de;
Arquivos de Neuro-Psiquiatria , 2002, DOI: 10.1590/S0004-282X2002000100007
Abstract: the aim of this study was to evaluate the effect of the ascorbic acid (aa) supplementation on the neurons that produce the vasoactive intestinal peptide (vip) in the submucous plexus of the ileum of rat, four months after the induction of experimental diabetes mellitus with streptozotocin. three groups of rats were used: c - control, d - diabetic, da - diabetic receiving aa. we have measured the immunoreactivity and area of 80 cellular bodies of vip-ergic neurons from each studied group. in the diabetic animals, we have observed hyperphagia, polydipsia, and an increase of glycemia and glycated hemoglobin. the vip-ergic neurons have presented an increase of their immunoreactivity and the highest profiles when compared to the other groups. in the diabetic animals supplemented with aa it has been observed a small reduction in the glycemia and the water and food intake. we have also noticed smaller immunoreactivity in their vip-ergic neurons, similar to what we have observed in the control group animals (group c).
Streptozotocin-induced diabetes duration is important to determine changes in the number and basophily of myenteric neurons
HERNANDES, LUZMARINA;BAZOTTE, ROBERTO BARBOSA;GAMA, PATRíCIA;MIRANDA-NETO, MARCíLIO HUBNER DE;
Arquivos de Neuro-Psiquiatria , 2000, DOI: 10.1590/S0004-282X2000000600010
Abstract: the aim of present study was to evaluate the number and basophily of cell bodies of myenteric neurons in the ileum of rats with diabetes mellitus induced by streptozotocin. four groups of rats were used: diabetes was induced in two (d) whereas the other two worked as controls (n). animals were sacrificed six (6n, 6d) or nineteen (19n, 19d) weeks after diabetes induction. a segment of the terminal portion of the ileum of each rat was obtained and stained with giemsa's solution, for whole-mount preparation studies. forty fields were analyzed in each animal, and the number and basophily intensity of cell bodies were recorded. after counting, the following mean numbers of neurons/mm2 were obtained: 6n=593.1 ± 95.75, 6d=639.1 ± 130.8, 19n=580.1 ± 175.6 and 19d=402.0 ± 144.8. the analysis of basophily shown that highest frequency of neurons with weak/intermediary basophily was verified in 6d group (55.3%), whereas the groups 6n, 19n e 19d presented 38%, 36% e 40% respectively. the statistical analysis showed that a long period is necessary to decrease the number of neurons/mm2 in the rat ileum after diabetes induction, and that there was a reduction in basophily intensity in diabetic rats after 6 weeks of treatment, and such cells do not recover after a longer period (19 weeks).
STUDY OF THE MYENTERIC PLEXUS OF THE ILEUM OF RATS SUBJECTED TO PROTEIC UNDERNUTRITION
Meilus,Marlos; Natali,Maria Raquel Mar?al; Miranda Neto,Marcílio Hubner de;
Revista chilena de anatomía , 1998, DOI: 10.4067/S0716-98681998000100002
Abstract: con el objetivo de verificar los efectos de la desnutrición proteica sobre el plexo mientérico del íleon, fueron utilizados 20 ratas del linaje wistar, cuyas madres fueron desnutridas en los períodos de gestación y/o lactacia sometidas a sacrificio a los 60 días de edad. se realizaron preparados de membrana del íleon te?idos con giemsa, para observación de las neuronas mientéricas y posteriores análisis y cuantificación. verificamos que la desnutrición proteica no provoca redución en el número de neuronas mientéricas por cm2 de íleon, y que las neuronas medias con basofilia intermediaria predominan en todos los grupos
STUDY OF THE MYENTERIC PLEXUS OF THE ILEUM OF RATS SUBJECTED TO PROTEIC UNDERNUTRITION ESTUDIO DEL PLEXO MIENTERICO DEL ILEON DE RATAS SOMETIDAS A DESNUTRICION PROTEICA
Marlos Meilus,Maria Raquel Mar?al Natali,Marcílio Hubner de Miranda Neto
Revista chilena de anatomía , 1998,
Abstract: Con el objetivo de verificar los efectos de la desnutrición proteica sobre el plexo mientérico del íleon, fueron utilizados 20 ratas del linaje Wistar, cuyas madres fueron desnutridas en los períodos de gestación y/o lactacia sometidas a sacrificio a los 60 días de edad. Se realizaron preparados de membrana del íleon te idos con GIEMSA, para observación de las neuronas mientéricas y posteriores análisis y cuantificación. Verificamos que la desnutrición proteica no provoca redución en el número de neuronas mientéricas por cm2 de íleon, y que las neuronas medias con basofilia intermediaria predominan en todos los grupos
MORPHOMETRY AND QUANTIFICATION OF THE MYENTERIC NEURONS OF THE DUODENUM OF ADULT RATS FED WITH HYPOPROTEIC CHOW MORFOMETRíA Y CUANTIFICACIóN DE LAS NEURONAS MIOENTéRICAS DEL DUODENO EN RATONES ADULTOS ALIMENTADOS CON RACIóN HIPOPROTEICA
Maria Raquel Mar?al Natali,Marcílio Hubner de Miranda-Neto,Ant?nio Marcos Orsi
International Journal of Morphology , 2003,
Abstract: Morphometric and quantitative analyses were carried out on the myenteric neurons of the duodenum, to study possible alterations resulting from a supply of low-protein level chow to adult Wistar rats. The animals were divided in two groups: nourished (N) fed with ration containing 22% protein for 210 days and the undernourished (D) fed with ration containing 8% protein from the 90th day of age on and during the next 120 days. Whole-mounts were stainedby the method of Giemsa and the histochemical reaction of NADH-diaphorase enzyme, to estimate the neuronal population, which revealed a greater neuronal density in undernourished rats, regardless of the technique employed. The morphometric studies of the cell body profiles of 500 neurons of each group indicated a reduction on the cell body size and an increase on the proportion of small neurons on the rats subjected to the hypoproteic chow En el plexo mioentérico del duodeno de ratones Wistar albinos adultos, se realizaron análisis morfológicos y cuantitativos para estudiar posibles alteraciones en el transcurso de su alimentación con raciones de bajo contenido de proteínas. Los animales fueron divididos en dos grupos: normoalimentados (N) cuya ración contenía 22% de proteínas y fueron alimentados con ella por 210 días, y el grupo desnutrido (D) alimentado con ración conteniendo 8% de proteínas, a partir de los 90 días de edad y durante los 120 días subsiguientes. Los micropreparados de membrana fueron te idos con el método GIEMSA, y a través de la reacción histoquímica de la enzima NADH-diaphorasa se estimó la población neuronal, que reveló mayor densidad neuronal en los animales desnutridos, independientemente de la técnica empleada. Los estudios morfométricos de los perfiles de los cuerpos celulares de 500 neuronas de cada grupo, indicaron reducción en el tama o del cuerpo celular y aumento de la proporción de neuronas peque as en los ratones alimentados con ración hipoproteica
Streptozotocin-induced diabetes duration is important to determine changes in the number and basophily of myenteric neurons
HERNANDES LUZMARINA,BAZOTTE ROBERTO BARBOSA,GAMA PATRíCIA,MIRANDA-NETO MARCíLIO HUBNER DE
Arquivos de Neuro-Psiquiatria , 2000,
Abstract: The aim of present study was to evaluate the number and basophily of cell bodies of myenteric neurons in the ileum of rats with diabetes mellitus induced by streptozotocin. Four groups of rats were used: diabetes was induced in two (D) whereas the other two worked as controls (N). Animals were sacrificed six (6N, 6D) or nineteen (19N, 19D) weeks after diabetes induction. A segment of the terminal portion of the ileum of each rat was obtained and stained with Giemsa's solution, for whole-mount preparation studies. Forty fields were analyzed in each animal, and the number and basophily intensity of cell bodies were recorded. After counting, the following mean numbers of neurons/mm2 were obtained: 6N=593.1 ± 95.75, 6D=639.1 ± 130.8, 19N=580.1 ± 175.6 and 19D=402.0 ± 144.8. The analysis of basophily shown that highest frequency of neurons with weak/intermediary basophily was verified in 6D group (55.3%), whereas the groups 6N, 19N e 19D presented 38%, 36% e 40% respectively. The statistical analysis showed that a long period is necessary to decrease the number of neurons/mm2 in the rat ileum after diabetes induction, and that there was a reduction in basophily intensity in diabetic rats after 6 weeks of treatment, and such cells do not recover after a longer period (19 weeks).
Terminal ileum submucous plexus: Study of the VIP-ergic neurons of diabetic rats treated with ascorbic acid
Zanoni Jacqueline Nelisis,Hernandes Luzmarina,Bazotte Roberto Barbosa,Miranda Neto Marcílio Hubner de
Arquivos de Neuro-Psiquiatria , 2002,
Abstract: The aim of this study was to evaluate the effect of the ascorbic acid (AA) supplementation on the neurons that produce the vasoactive intestinal peptide (VIP) in the submucous plexus of the ileum of rat, four months after the induction of experimental diabetes mellitus with streptozotocin. Three groups of rats were used: C - control, D - diabetic, DA - diabetic receiving AA. We have measured the immunoreactivity and area of 80 cellular bodies of VIP-ergic neurons from each studied group. In the diabetic animals, we have observed hyperphagia, polydipsia, and an increase of glycemia and glycated hemoglobin. The VIP-ergic neurons have presented an increase of their immunoreactivity and the highest profiles when compared to the other groups. In the diabetic animals supplemented with AA it has been observed a small reduction in the glycemia and the water and food intake. We have also noticed smaller immunoreactivity in their VIP-ergic neurons, similar to what we have observed in the control group animals (group C).
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