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Search Results: 1 - 10 of 3923 matches for " Manesh Shah "
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Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose
Kumaran Sivagnanam, Vijaya GS Raghavan, Manesh Shah, Robert L Hettich, Nathan C Verberkmoes, Mark G Lefsrud
Proteome Science , 2011, DOI: 10.1186/1477-5956-9-66
Abstract: We identified 894 different proteins in C. acetobutylicum from ABE fermentation process by two dimensional - liquid chromatography - tandem mass spectrometry (2D-LC-MS/MS) method. This includes 717 proteins from glucose and 826 proteins from the xylose substrate. A total of 649 proteins were found to be common and 22 significantly differentially expressed proteins were identified between glucose and xylose substrates.Our results demonstrate that flagellar proteins are highly up-regulated with glucose compared to xylose substrate during ABE fermentation. Chemotactic activity was also found to be lost with the xylose substrate due to the absence of CheW and CheV proteins. This is the first report on the shotgun proteomic analysis of C. acetobutylicum ATCC 824 in ABE fermentation between glucose and xylose substrate from a single time data point and the number of proteins identified here is more than any other study performed on this organism up to this report.Clostridium acetobutylicum is a gram positive, spore forming, obligate anaerobic bacteria and is one of the few microorganisms capable of converting a wide variety of sugars into three main products acetone, butanol and ethanol (ABE) [1]. ABE fermentation process was the primary source of butanol for over 40 years until the mid-1950s and is one of the oldest large-scale industrial fermentations [2]. ABE fermentation could not compete with the chemical synthesis of ABE solvents from petroleum since the mid-1950s [3]. However, increased concern over depletion of fossil fuels has led to renewed research interest in producing solvents via microbial fermentation processes.Lignocellulosic biomass is an abundant renewable resource that can be used for the production of alternative fuels [4]. It is advantageous to use lignocellulosic biomass such as rice straw, wheat straw, corn stover and agricultural residues for biofuel production as they have limited impact on food supplies [5]. Glucose is the most abundant sugar fou
Risk Factors of First Acute Myocardial Infarction: Comparison of Elderly and Non-Elderly: A 24-Year Study  [PDF]
Soheila Dabiran, Behrooz Khaleghi Manesh, Farahnaz Khajehnasiri
Advances in Aging Research (AAR) , 2015, DOI: 10.4236/aar.2015.41002
Abstract: Although risk factors of Ischemic Heart Disease (IHD) are well known, knowledge about the distribution of these risk factors in different subgroups of patients can be used for designing preventive programs. The aim of this study was to assess differences of prevalence of IHD risk factors in elderly and non-elderly patients in their first Acute Myocardial Infarction (AMI). In this retros-pective study, elderly (age > 65; n = 1060) and non-elderly (age ≤ 65; n = 2228) patients with first AMI who were admitted into four teaching hospitals in Tehran between 1982 and 2005 were recruited. Risk factors containing male gender, systolic and diastolic hypertension (HTN), diabetes, hypertriglyceridemia, hypercholesterolemia, smoking and family history of IHD were compared between the two groups. Among different risk factors, diabetes and systolic HTN were more common in elderly than their non-elderly counterparts, and male gender, smoking, family history of IHD and dyslipidemia were more common in non-elderly patients than elderly ones (p < 0.05 for all comparison). Diastolic HTN showed no significant difference between the two groups. Findings of this study, stating difference of prevalence of risk factors in two elderly and non-elderly groups, can be used by health policy makers to conduct preventive programs for AMI in this country.
Phage-Induced Expression of CRISPR-Associated Proteins Is Revealed by Shotgun Proteomics in Streptococcus thermophilus
Jacque C. Young, Brian D. Dill, Chongle Pan, Robert L. Hettich, Jillian F. Banfield, Manesh Shah, Christophe Fremaux, Philippe Horvath, Rodolphe Barrangou, Nathan C. VerBerkmoes
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0038077
Abstract: The CRISPR/Cas system, comprised of clustered regularly interspaced short palindromic repeats along with their associated (Cas) proteins, protects bacteria and archaea from viral predation and invading nucleic acids. While the mechanism of action for this acquired immunity is currently under investigation, the response of Cas protein expression to phage infection has yet to be elucidated. In this study, we employed shotgun proteomics to measure the global proteome expression in a model system for studying the CRISPR/Cas response in S. thermophilus DGCC7710 infected with phage 2972. Host and viral proteins were simultaneously measured following inoculation at two different multiplicities of infection and across various time points using two-dimensional liquid chromatography tandem mass spectrometry. Thirty-seven out of forty predicted viral proteins were detected, including all proteins of the structural virome and viral effector proteins. In total, 1,013 of 2,079 predicted S. thermophilus proteins were detected, facilitating the monitoring of host protein synthesis changes in response to virus infection. Importantly, Cas proteins from all four CRISPR loci in the S. thermophilus DGCC7710 genome were detected, including loci previously thought to be inactive. Many Cas proteins were found to be constitutively expressed, but several demonstrated increased abundance following infection, including the signature Cas9 proteins from the CRISPR1 and CRISPR3 loci, which are key players in the interference phase of the CRISPR/Cas response. Altogether, these results provide novel insights into the proteomic response of S. thermophilus, specifically CRISPR-associated proteins, upon phage 2972 infection.
Proteomics reveals a core molecular response of Pseudomonas putida F1 to acute chromate challenge
Dorothea K Thompson, Karuna Chourey, Gene S Wickham, Stephanie B Thieman, Nathan C VerBerkmoes, Bing Zhang, Andrea T McCarthy, Matt A Rudisill, Manesh Shah, Robert L Hettich
BMC Genomics , 2010, DOI: 10.1186/1471-2164-11-311
Abstract: Growth studies demonstrated that F1 sensitivity to Cr(VI) was impacted substantially by nutrient conditions, with a carbon-source-dependent hierarchy (lactate > glucose >> acetate) observed in minimal media. Two-dimensional HPLC-MS/MS was employed to identify differential proteome profiles generated in response to 1 mM chromate under LB and M9L growth conditions. The immediate response to Cr(VI) in LB-grown cells was up-regulation of proteins involved in inorganic ion transport, secondary metabolite biosynthesis and catabolism, and amino acid metabolism. By contrast, the chromate-responsive proteome derived under defined minimal growth conditions was characterized predominantly by up-regulated proteins related to cell envelope biogenesis, inorganic ion transport, and motility. TonB-dependent siderophore receptors involved in ferric iron acquisition and amino acid adenylation domains characterized up-regulated systems under LB-Cr(VI) conditions, while DNA repair proteins and systems scavenging sulfur from alternative sources (e.g., aliphatic sulfonates) tended to predominate the up-regulated proteome profile obtained under M9L-Cr(VI) conditions.Comparative analysis indicated that the core molecular response to chromate, irrespective of the nutritional conditions tested, comprised seven up-regulated proteins belonging to six different functional categories including transcription, inorganic ion transport/metabolism, and amino acid transport/metabolism. These proteins might potentially serve as indicators of chromate stress in natural microbial communities.Pseudomonas putida is a ubiquitous gram-negative, saprophytic bacterium belonging to the gamma class of the Proteobacteria. Endowed with a remarkable environmental adaptability, P. putida strain F1 [1], for example, has been investigated most extensively as a model organism for the microbial degradation of such xenobiotic aromatic compounds as toluene, benzene, and ethylbenzene [2]. Considerably less scientific focus
Theories of Equity in Health Care, Implications for Developed and Developing Countries
AR Olyaee Manesh
Iranian Journal of Public Health , 2005,
Abstract: Objective: This abstract focuses on theoretical background for the notion of “equity in health care” and on the implications of applying these theories to the health care of developed and developing countries. Equity Principals and the Implications: There are different theories about the principle of Equity or Justice such as Altruism, Utilitarians, Marxists, Rawls’ theory and Nazick’s entitlement. Among these theories, some of them are applicable to health care such as Libertarianism and Egalitarianism. The focus of the Libertarians is on the extent to which people are free to purchase the health care that they want. The Libertarians’ principle is the main equity base for private health systems. In contrast, Egalitarians suggest that finance of the health care should be according to the ability to pay and distribution of health care should be according to the need (ill health). It seems that policy makers in most developed countries such as European countries accept the Egalitarians’ principle and application of this equity principle by their health systems has significantly reduced health inequities and inequalities in these countries. There are a limited number of studies to look at equity in the health care of developing countries. A common equity principle for these countries is “equal access for everyone” and different mechanisms are applied to attain this target. Despite the overall improvements in health care in recent years, evidence indicates that many of developing countries have failed to provide equal access to health care for all in need. The financial limitations of the governments, spending about 70% of the health care resources on hospital-based care, unequal access to hospital services in favour of urban population, income inequalities among population, and lack of consistent and up-to-date information of inequalities, make developing countries unable to monitor and prevent inequities and inequalities of health care. Conclusion: The main equity principle in the health care of developed countries is Egalitarians’ principle in which, health care should be financed according to ability to pay and should be delivered according to the need. Applying the equity principals to the health care of developed countries has deeply promoted equitable health care in these countries. There are a limited number of studies about this issue in developing countries. A common equity target in these countries is “equal access for everyone in need” which is close to the Egalitarians’ point of view. However, some factors have hampered these countries’ attempt
A Method of Detecting Sql Injection Attack to Secure Web Applications
Sruthy Manmadhan,Manesh T
International Journal of Distributed and Parallel Systems , 2012,
Abstract: Web applications are becoming an important part of our daily life. So attacks against them also increasesrapidly. Of these attacks, a major role is held by SQL injection attacks (SQLIA). This paper proposes anew method for preventing SQL injection attacks in JSP web applications. The basic idea is to checkbefore execution, the intended structure of the SQL query. For this we use semantic comparison. Ourfocus is on stored procedure attack in which query will be formed within the database itself and sodifficult to extract that query structure for validation. Also this attack is less considered in the literature.
The Ups and Downs of Sex Life in Menopausal Stage: a Qualitative Study
M Jamshidi Manesh
Qom University of Medical Sciences Journal , 2012,
Abstract: Background and Objectives: The meaning of menopause and women experience of this transitional stage are related to cultural norms, social factors and one's knowledge of menopause. Women’s image of menopasuse affects self-care and follow-ups during this stage. Among them is consideration of sexuality which is often ignored. The aim of this study was to discover menopausal complications in Iranian women. Methods: 14 women in menopausal stage were interviewed about their clinical experience. The interviews were transcribed and then analyzed using Van Manen’s phenomenology technique. Result: Data analysis showed that one of the women's most important clinical experiences of menopause which they can deliberately express was the start of a new stage. Since, according to those women, changes in their life style were being made in comparison with earlier stages, one of the most important problems that escaped unobserved was sexual challenges which started at this stage. Conclusion: The findings this study reveal women's beliefs about their sex life. This can be used as a basis for education and offering guidelines in order to increase their quality of life.
Accuracy of Endosonography in the Diagnosis of Obstructive Jaundice
R Sotoudeh Manesh
Qom University of Medical Sciences Journal , 2012,
Abstract: Background and Objectives: Endoscopic retrograde cholangiopancreatography (ERCP) has been used as the method of choice in assessment of obstructive jaundice for a long time. Recently, the development of endoscopic ultrasonography (EUS) has attracted the attention of clinicians as an alternative accurate, safe and cheap method to replace the ERCP procedures performed only for diagnostic purposes. Methods: In this study, 100 patients underwent endoscopic ultrasonography in Digestive Disease Research Center, Shariati Hospital. The patients were divided into two groups: 1) those with operable periampullary tumors who were referred to the surgeons, 2) other patients. The final diagnosis made after ERCP and operative removal of the mass was considered as the gold standard and was compared with accuracy of endoscopic ultrasonography. Results: The overall accuracy of endoscopic ultrasonography in the diagnosis of obstructive cholestasis was 92.0%. Sensitivity and specificity of this method in diagnosis of choledocholithiasis were 95.2% and 96.4% respectively. The corresponding values for diagnosis of periampullary tumors were 96.2% and 95.8%. Conclusion: In this study the accuracy of EUS in diagnosis of obstructive jaundice and its sensitivity and specificity for diagnosis of choledocholithiasis and periampullary tumors were high. Therefore, it could be concluded that endoscopic ultrasonography is a reliable diagnostic tool for extrahepatic cholestasis.
Influence of poly germander (Teucrium polium) and watercress (Nasturtium officinale) extract on performance, carcass quality and blood metabolites of male broilers
Mostaan Khosravi Manesh
Research Opinions in Animal & Veterinary Sciences , 2012,
Abstract: This experiment was conducted to study effect of poly germander (Teucrium polium) and watercress (Nasturtium officinale) extracts on performance and serum biochemistryl of broilers. Two hundred and forty male Ross broilers (were alloted into 4 diet treatments for 42 days. The birds were fed their standard feed without any addition (control) or with addition of 200 ppm of poly germander extract (T1), 200 ppm watercress extract (T2) or 200 ppm of the combination of both components (T4). No significant change was observed in term of growth parameters and blood biochemistry, however, abdominal fat decreased significantly in the watercress and the combination extracts supplemented groups. The study highlighted the potential of these herbal extracts to improve body performance and carcass quality of broilers.
Optimization of Bit Plane Combination for Efficient Digital Image Watermarking
Sushma Kejgir,Manesh Kokare
Computer Science , 2009,
Abstract: In view of the frequent multimedia data transfer authentication and protection of images has gained importance in todays world. In this paper we propose a new watermarking technique, based on bit plane, which enhances robustness and capacity of the watermark, as well as maintains transparency of the watermark and fidelity of the image. In the proposed technique, higher strength bit plane of digital signature watermark is embedded in to a significant bit plane of the original image. The combination of bit planes (image and watermark) selection is an important issue. Therefore, a mechanism is developed for appropriate bit plane selection. Ten different attacks are selected to test different alternatives. These attacks are given different weightings as appropriate to user requirement. A weighted correlation coefficient for retrieved watermark is estimated for each of the alternatives. Based on these estimated values optimal bit plane combination is identified for a given user requirement. The proposed method is found to be useful for authentication and to prove legal ownership. We observed better results by our proposed method in comparison with the previously reported work on pseudorandom watermark embedded in least significant bit (LSB) plane.
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