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Objective: To study
a comprehensive proteomic analysis of celecoxib in oseteoarthritis (OA) chondrocytes.
Methods: OA chondrocytes were stimulated with celecoxib, IL-1β and IL-1β together with
celecoxib. Proteins were extracted from the cells and subjected to
2-dimensional differential image gel electrophoresis (2D-DIGE). Proteins of
interest were identified by mass spectrometry. Results: Eighty-six protein
spots showed significantly different intensities with each reagent or reagent
combination. AAA+ protein, HSP47/Serpin, cAMP-dependent protein kinase type
II-beta regulatory subunit, alpha-actin-4 and tubulin decreased with the
addition of celecoxib, while apolipoprotein A-V, glutamate carboxipeptide 2,
mitochondrial stress-70 protein, sorting nexin-9 and GRP78 increased with the
addition of celecoxib. GRP78 is a stress protein and may be chondroprotective.
Celecoxib modulated IL-1β stimulated
chondrocytes, and CD200R and moesin were identified as such resulting proteins.
Conclusion: Protein profiles of OA chondrocytes changed after administration of
celecoxib. Further investigation is needed to elucidate the function of each
protein in OA chondrocytes.
The objective of this paper is to show an alternative model of a non-transposed three-phase transmission line with a vertical symmetry plane in phase domain. Due the line physical characteristics, it can be represented by a system consisting of a single?phase and a two-phase line. In this system, the equations
describing the behavior of the values in single-phase line terminals are known, while the equations for two-phase line to be obtained. Using a transformation matrix written
explicitly according to three-phase line
parameters, it is possible to obtain
the currents and voltages in phase domain of two-phase line.
Then, modal values of three-phase line are converted into phase domain
and thus obtain
This paper shows the development of transmission line
model, based on lumped element circuit that provides answers directly
in the time and phase domain. This
model is valid to represent the ideally transposed line, the phases of each of
the small line segments are separated in their modes of propagation and the
voltage and current are calculated at the modal domain. However, the conversion
phase-mode-phase is inserted in the state equations which describe the currents
and voltages along the line of which there is no need to know the user of the
model representation of the theory in the line modal domain.
The second-order differential equations that describe
the transmission line are difficult to solve due to the mutual coupling among
phases and the fact that the parameters are distributed along their length. A
method for the analysis of polyphase systems is the technique that decouples
their phases. Thus, a system that has n phases coupled can be represented by n
decoupled single-phase systems which are mathematically identical to the
original system. Once obtained the n-phase circuit, it’s possible to calculate
the voltages and currents at any point on the line using computational methods.
The Universal Line Model (ULM) transforms the differential equations in the time domain
to algebraic equations in the frequency domain, solve them and obtain the
solution in the frequency domain using the inverse Laplace
transform. This work will analyze the method of modal decomposition in a
three-phase transmission line for the calculation of voltages and currents of
the line during the energizing process.
Purpose: Little research has been reported to date on the usefulness of olprinone in pediatric cardiac surgery, and no standard pediatric infusion protocol is currently established. Our study sought to confirm that the regimen described herein rapidly achieves the requisite plasma olprinone concentrations. Methods: For the purposes of our study, we enrolled 13 patients: 7 biventricular repair candidates and 6 Fontan-type operation candidates. We administered a continuous infusion of olprinone to our study subjects at 0.3 μg/kg/min with no loading dose starting approximately 30 minutes (min) before weaning from cardiopulmonary bypass (CPB). We performed blood sampling at 15, 30, 45, 60, 90, and 120 min after the start of infusion and at the same elapsed intervals after separation from CPB. We measured plasma olprinone concentrations using ultra-fast liquid chromatography. Results: We observed effective plasma olpri-none concentrations (>20 ng/ml) at 30 min after weaning from CPB, or at 60 min after the start of infusion. Conclusion: We conclude that continuous olprinone infusion at 0.3 μg/kg/min without a loading dose initiated immediately after the release of aortic cross-clamping or immediately after the completion of all surgical procedures quickly and reliably achieves effective plasma concentrations.