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Oxidative stress has been identified as one of the factors that affects fertility status. Therefore, this study sought to investigate the inhibitory effect of aqueous extract of Moringa oleifera and Newbuoldia laevis leaves on FeSO4 and Sodium Nitroprusside (SNP) induced lipid peroxidation in rat testes in vitro. Incubation of the testes tissue homogenate in the presence of FeSO4 and SNP caused a significant increase in the malondialdehyde (MDA) contents of the testes. The aqueous extract from both Moringa oleifera and Newbuoldia laevis leaves caused a significant decrease in the MDA contents of the testes in a dose-dependent manner. However, aqueous extract from Moringa oleifera leaf (EC50 = 0.29 mg/ml) had a significant (P<0.05) higher inhibitory effect on Fe2+ induced lipid peroxidation in the rat testes homogenate than that of Newbuoldia laevis leaf extract (EC50 = 0.58 mg/ml); while there was no significant (P<0.05) difference between the plant extracts on SNP induced lipid peroxidation in the rat testes homogenates. Therefore, part of the mechanisms through which the water extractable phytochemicals in the leaves protect the testes from oxidative stress may be through their antioxidant activity; DPPH scavenging ability, Fe2+ chelating and reducing power. Therefore, these plants have potential to prevent oxidative stress in testes and improve fertility outcomes.
Objective: Oral mucositis (OM) is a devastating toxicity associated with cytotoxic cancer therapy. The OM pathogenesis and the complex interactions occur in response to tissue insult. Application of this evolving model has aided in the development of mechanistically based therapies for the prevention and treatment of mucositis. The present study was to assess the effects of glycine supplementation on chemotherapy-induced oral mucositis. Methods: In a hamster cheek pouch model of chemotherapy-induced oral mucositis, one group of 20 animals received systemic glycine supplementation for 7 days, while another similar control group did not. Clinical mucositis severity and neutrophil infiltrate (on histology) were assessed by blinded examiners. Free radical production was measured as malondialdehyde (MDA) levels. Results: As compared to control animals, glycine-treated animals demonstrated a highly significant reduction in clinical severity of oral mucositis, neutrophil infiltrate, and MDA levels (p < 0.001 for all). Conclusions: Glycine supplementation reduces the severity of chemotherapy-induced oral mucositis in an animal model. This effect is at least partly mediated through inhibition of the inflammatory response and reduced production of damaging free radicals.
The aim of this
work was to evaluate the alleviating efficacy of salicylic acid (100, 250 and
500 μM) against the toxic effects of aluminum (Al) on two tomato cultivars
(GOWRI and SIRI) differing in their sensitivity to Al stress. Al treatment (500
μM) caused 40% - 80% drop in plant growth, relative water content (RWC) and
cell viability and a reduction of 1 - 2.5 fold and 0.5 - 2 fold in glutathione
and proline content respectively, when compared to their control plants grown
in Al free medium. Al treatment also resulted in 2 - 5 fold raise in
malondialdehyde (MDA) levels, 2 - 3 fold higher Al uptake and 55% - 80% more
electrolyte leakage and caused severe DNA damage. Al stress enhanced (1 - 2
fold) the activities of superoxide dismutase (SOD) and peroxidase (POD), but
decreased catalase (CAT) activity over their respective controls.
Exogenously-applied Salicylic acid (SA) significantly (p < 0.05) and dose-dependently alleviated Al-induced toxicity in
tomato seedlings as marked by much improved plant growth, retention of higher
RWC, cell viability, glutathione and proline content. SA also caused 0.6 - 1.5
fold reduction in Al uptake, 50% - 80% less electrolyte leakage, 40% - 80% drop
in lipid peroxidation and considerable protection against DNA damage. Also,
supplementation of SA could considerably reverse the Al-induced changes in the
activities of SOD, POD and CAT. Together, our findings demonstrate that, SA is
an efficient growth regulator with diversified roles that contribute to its
potential alleviating effect against Al induced toxicity and SIRI is a
relatively Al-resistant cultivar compared to GOWRI.