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Search Results: 1 - 10 of 27 matches for " Malinee Sriariyanun "
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Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium
Sang-Wook Han, Malinee Sriariyanun, Sang-Won Lee, Manoj Sharma, Ofir Bahar, Zachary Bower, Pamela C. Ronald
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0029192
Abstract: The rice XA21 pattern recognition receptor binds a type I secreted sulfated peptide, called axYS22, derived from the Ax21 (activator of XA21-mediated immunity) protein. The conservation of Ax21 in all sequenced Xanthomonas spp. and closely related genera suggests that Ax21 serves a key biological function. Here we show that the predicted N-terminal sequence of Ax21 is cleaved prior to secretion outside the cell and that mature Ax21 serves as a quorum sensing (QS) factor in Xanthomonas oryzae pv. oryzae. Ax21-mediated QS controls motility, biofilm formation and virulence. We provide genetic evidence that the Xoo RaxH histidine kinase serves as the bacterial receptor for Ax21. This work?establishes a critical role for small protein-mediated QS in a Gram-negative bacterium.
A two-genome microarray for the rice pathogens Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola and its use in the discovery of a difference in their regulation of hrp genes
Young-Su Seo, Malinee Sriariyanun, Li Wang, Janice Pfeiff, Jirapa Phetsom, Ye Lin, Ki-Hong Jung, Hui Hsien Chou, Adam Bogdanove, Pamela Ronald
BMC Microbiology , 2008, DOI: 10.1186/1471-2180-8-99
Abstract: Three biological replicates of the microarray experiment to compare global gene expression in representative strains of Xoo and Xoc grown in PSB vs. XOM2 were carried out. The non-specific error rate and the correlation coefficients across biological replicates and among duplicate spots revealed that the microarray data were robust. 247 genes of Xoo and 39 genes of Xoc were differentially expressed in the two media with a false discovery rate of 5% and with a minimum fold-change of 1.75. Semi-quantitative-RT-PCR assays confirmed differential expression of each of 16 genes each for Xoo and Xoc selected for validation. The differentially expressed genes represent 17 functional categories.We describe here the construction and validation of a two-genome microarray for the two pathovars of X. oryzae. Microarray analysis revealed that using representative strains, a greater number of Xoo genes than Xoc genes are differentially expressed in XOM2 relative to PSB, and that these include hrp genes and other genes important in interactions with rice. An exception was the rax genes, which are required for production of the host resistance elicitor AvrXa21, and which were expressed constitutively in both pathovars.The rice pathogens Xanthomonas oryzae pathovar oryzae (Xoo) and Xanthomonas oryzae pathovar oryzicola (Xoc) cause economically significant disease in many rice-growing regions of the world [1]. Xoo invades rice vascular tissue to cause bacterial leaf blight, whereas Xoc colonizes the mesophyll parenchyma tissue to cause bacterial leaf streak. Xoo gains access to the xylem through wounds or natural openings such as hydathodes, while Xoc, in contrast, enters the leaf mainly through stomata [2]. Xoo and Xoc are closely related, infect the same host, and are often both established in the same rice fields. The complete genome sequences of Japanese Xoo strain T7174 (also called MAFF311018) and Korean Xoo strain KACC10331 have been published [3,4]. The genome sequences of a t
Effect of borax on immune cell proliferation and sister chromatid exchange in human chromosomes
Malinee Pongsavee
Journal of Occupational Medicine and Toxicology , 2009, DOI: 10.1186/1745-6673-4-27
Abstract: The heparinized blood samples from 40 healthy men were studied for the impact of borax toxicity on immune cell proliferation (lymphocyte proliferation) and sister chromatid exchange in human chromosomes. The MTT assay and Sister Chromatid Exchange (SCE) technic were used in this experiment with the borax concentrations of 0.1, 0.15, 0.2, 0.3 and 0.6 mg/ml.It showed that the immune cell proliferation (lymphocyte proliferation) was decreased when the concentrations of borax increased. The borax concentration of 0.6 mg/ml had the most effectiveness to the lymphocyte proliferation and had the highest cytotoxicity index (CI). The borax concentrations of 0.15, 0.2, 0.3 and 0.6 mg/ml significantly induced sister chromatid exchange in human chromosomes (P < 0.05).Borax had effects on immune cell proliferation (lymphocyte proliferation) and induced sister chromatid exchange in human chromosomes. Toxicity of borax may lead to cellular toxicity and genetic defect in human.Borax (Na2B4O2(H2O)10) is a low toxicity mineral with insecticidal, fungicidal and herbicidal properties. The basic structure of borax contains chains of interlocking BO2(OH) triangles and BO3(OH) tetrahedrons bonded to chains of sodium and water octahedrons. Borax occurs naturally in evaporate deposits produced by the repeated evaporation of seasonal lakes. It is a precursor for sodium perborate monohydrate that is used in detergents. Borax can be produced synthetically from other boron compounds. It is usually a white powder consisting of soft colorless crystals that dissolve easily in water. Borax is used in detergents and cosmetics, as an ingredient in enamel glazes, glass, pottery, and ceramics, to make buffer solutions. It is used mixed with water as a flux when soldering jewelry metals such as god or silver [1].Borax has the toxicity to humans, including reproductive and developmental toxicity, neurotoxicity, and nephrotoxicity. The degree of borax toxicity depends on the dose or concentration that the
Impact of water temperature and sodium chloride (NaCl) on stress indicators of hybrid catfish (Clarias gariepinus Burchell x C. macrocephalus Gunther)
Weena Koeypudsa,Malinee Jongjareanjai
Songklanakarin Journal of Science and Technology , 2011,
Abstract: This research was composed of 2 experiments. Short-term (1 day) and long-term (30 days) exposure were conductedin the laboratory. Each experiment had 2 temperature levels, high (Ht, 29.5 ± 0.5 C) and low temperature (Lt, 19.5 ± 0.5 C).Initial weight and length of catfish were 7.54±1.82 g and 9.90±0.96 cm respectively. Experimental catfish were subjected to 4conditions as follows: high temperature with 0.1% sodium chloride (HtWs), high temperature without 0.1% sodium chloride(HtW/s), low temperature with 0.1% sodium chloride (LtWs), and low temperature without 0.1% sodium chloride (LtW/s).Blood was taken from caudal vessel of anaesthetized fish to investigate blood clotting time, cortisol, glucose, osmolarity,Na+, K+ and Cl-. Ratios of Na+/K+, Na+/Cl- and Na+ + K+ / Cl- were also analyzed. In the short-term (1 day) experiment, values ofall catfish blood parameters varied. These imply that catfish attempt to maintain internal balance, homeostasis. Osmolarityexhibited complete homeostasis in 2 h. From long-term (30 days) exposure, non-significant means of Na+/Cl- ratio (HtWs)and decreasing trend lines direction of blood clotting time (HtWs, LtW/s, LtWs) indicated that 0.1% sodium chloride and/orLt helped stress reduction in catfish. Sum of Na+ and K+ to Cl- ratio among 4 groups (HtW/s, HtWs, LtW/s, LtWs) revealedthat catfish spent 10 days for adjustment themselves under stress circumstance (HtWs, LtW/s, LtWs) to natural situation(HtW/s). This information could be useful to improve the survival rate and health condition during rearing, handling andtransporting aquatic animals.
Pathological Study of Blood Parasites in Rice Field Frogs, Hoplobatrachus rugulosus (Wiegmann, 1834)
Achariya Sailasuta,Jetjun Satetasit,Malinee Chutmongkonkul
Veterinary Medicine International , 2011, DOI: 10.4061/2011/850568
Abstract: One hundred and forty adult rice field frogs, Hoplobatrachus rugulosus (Wiegmann, 1834), were collected in Srakaew province, Thailand. For blood parasite examination, thin blood smears were made and routinely stained with Giemsa. The results showed that 70% of the frogs (98/140) were infected with 5 species of blood parasites, including a Trypanosoma rotatorium-like organism, Trypanosoma chattoni, Hepatozoon sp. a, Hepatozoon sp. b, and Lankesterella minima. Pathological examination of the liver, lung, spleen, and kidney of the frogs that were apparently infected with one of these blood parasites were collected and processed by routine histology and subsequently stained with haematoxylin and eosin. Histopathological findings associated with the Trypanosoma rotatorium-like organism and Trypanosoma chattoni-infected frogs showed no pathological lesions. Hepatozoon sp. a and Hepatozoon sp. b-infected frogs developed inflammatory lesions predominantly in the liver, demonstrating granuloma-like lesions with Hepatozoon sp. meronts at the centre. Tissue sections of Lankesterella minima-infected frogs also showed lesions. Liver and spleen showed inflammatory lesions with an accumulation of melanomacrophage centres (MMCs) surrounding the meronts and merozoites. It is suggested that Hepatozoon sp. a, Hepatozoon sp. b, and Lankesterella minima-infections are capable of producing inflammatory lesions in the visceral organs of rice field frogs, and the severity of lesions is tentatively related to levels of parasitemia. 1. Introduction Studies in several geographical regions have indicated anurans infected with variety of blood parasites, including viruses, rickettsiae, several species of protozoans, and microfilariae [1–6]. Trypanosoma spp. (Euglenozoa: Kinetoplastida) are heteroxenous and vector transmitted through a variety of mechanisms. The classification of anuran trypanosomes is still in confusion because some species may be polymorphic [7–9]. Trypanosoma rotatorium is a polymorphic species with a wide geographical distribution, while Trypanosoma chattoni is a monomorphic species that is found in Asia, Europe, and America [7, 10]. Hepatozoon spp. (Apicomplexa: Hepatozoidae) are often found in amphibians, appearing as large banana-shaped organisms in the cytoplasm of host erythrocytes. Hepatozoon species possess heteroxenous life cycles, with sexual reproduction and sporogony occurring in an arthropod definitive host. Transmission occurs when such an arthropod, infected with mature oocysts, often containing thousands of sporozoites, is consumed by a vertebrate
Time to Update and Quantitative Changes in the Results of Cochrane Pregnancy and Childbirth Reviews
Wanlop Jaidee,David Moher,Malinee Laopaiboon
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0011553
Abstract: The recommended interval between updates for systematic reviews included in The Cochrane Library is 2 years. However, it is unclear whether this interval is always appropriate. Whereas excessive updating wastes time and resources, insufficient updating allows out-of-date or incomplete evidence to guide clinical decision-making. We set out to determine, for Cochrane pregnancy and childbirth reviews, the frequency of updates, factors associated with updating, and whether updating frequency was appropriate.
Functional Analysis of Familial Asp67Glu and Thr1051Ser BRCA1 Mutations in Breast/Ovarian Carcinogenesis
Malinee Pongsavee,Pimpicha Patmasiriwat,Grady F. Saunders
International Journal of Molecular Sciences , 2009, DOI: 10.3390/ijms10094187
Abstract: Estrogen is believed to be pre-initiator in the risk of breast cancer. The BRCA1 is a tumor suppressor gene associated with breast and ovarian cancer risk. This report describes functional analysis of two BRCA1 missense mutations (Asp67Glu and Thr1051Ser) observed in the familial breast/ovarian cancer patients in Thailand. Levels of luciferase activity of the two mutations were relatively lower than in the wild-type BRCA1. It is indicated that mutants may fail to promote the estrogen receptor dependent functions.It is presumed that estrogen and insulin/IGF-1 regulate c-Myc and cyclin D1 during breast cancer cell proliferation. It is also likely to affect ubiquitination mechanism. Since three affected cancer families carry the Asp67Glu mutation, it is believed that this type of mutation could have some effect on breast/ovarian cancer progression.
Capability of Emergency Medical Service Response Teams at Khon Kaen, Thailand
Ounjai Kruesathit,Malinee Laopaiboon,Witaya Chadbunchachai
Research Journal of Medical Sciences , 2012, DOI: 10.3923/rjmsci.2012.232.237
Abstract: To describe capability of response team provided vital care to patients and characteristic of EMS case. A descriptive study was done to describe capability of response team providing care among EMS patients from March 1 through August 31, 2009. EMS operation data was extracted from the database in November 2009. A total of 37,042 EMS case were analysed. The most of response team was BLS care as 95.5% (23.9% BLS and 71.6% FR team). The most of case was the urgent as 61.0% (95% CI 60.5-61.5%) and emergent as 8.0% (95% CI 7.7-8.3%). Capability of all teams on the airway and breathing, circulation control, immobilization and lifting and moving for patients were appropriate rate within team s competency of 83.4-100%. Capability in vital care of all EMS response teams in Khon Kaen, Thailand was highly appropriate rate within team competency. The most of response team was BLS care. Thus, the BLS care team should well train in essential skills to care for critical patient.
Chemical constituents and antioxidative activity of the extracts from Dyera costulata leaves
Sanan Subhadhirasakul,Bamrung Jankeaw,Apai Malinee
Songklanakarin Journal of Science and Technology , 2003,
Abstract: Chloroform and n-butanol extracts from the leaves of Dyera costulata exhibited EC50 values for DPPH radical scavenging activity of 79.8±0.2 and 12.0±0.1 μg/ml, respectively. Chemical investigation of the extracts was conducted by means of column chromatography. β-Amyrin and rhamnazin were isolated from the chloroform extract and quercetin-3-O-α-L-rhamnopyranoside was isolated from the n-butanol extract. The chemical structures of the isolated compounds were identified by comparison of their spectroscopic data with those reported in the literature. The EC50 value of quercetin-3-O-α-L-rhamnopyranoside in DPPH radical scavenging activity was 9.37±0.02 μM. Its antioxidative activity is therefore about 8 times higher than that of the well known antioxidant, BHT, which has an EC50 value of 80.78±0.01 μM.
A Comparison of Statistical Methods for Identifying Out-of-Date Systematic Reviews
Porjai Pattanittum, Malinee Laopaiboon, David Moher, Pisake Lumbiganon, Chetta Ngamjarus
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0048894
Abstract: Background Systematic reviews (SRs) can provide accurate and reliable evidence, typically about the effectiveness of health interventions. Evidence is dynamic, and if SRs are out-of-date this information may not be useful; it may even be harmful. This study aimed to compare five statistical methods to identify out-of-date SRs. Methods A retrospective cohort of SRs registered in the Cochrane Pregnancy and Childbirth Group (CPCG), published between 2008 and 2010, were considered for inclusion. For each eligible CPCG review, data were extracted and “3-years previous” meta-analyses were assessed for the need to update, given the data from the most recent 3 years. Each of the five statistical methods was used, with random effects analyses throughout the study. Results Eighty reviews were included in this study; most were in the area of induction of labour. The numbers of reviews identified as being out-of-date using the Ottawa, recursive cumulative meta-analysis (CMA), and Barrowman methods were 34, 7, and 7 respectively. No reviews were identified as being out-of-date using the simulation-based power method, or the CMA for sufficiency and stability method. The overall agreement among the three discriminating statistical methods was slight (Kappa = 0.14; 95% CI 0.05 to 0.23). The recursive cumulative meta-analysis, Ottawa, and Barrowman methods were practical according to the study criteria. Conclusion Our study shows that three practical statistical methods could be applied to examine the need to update SRs.
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