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Aim: To assess the role of Magnetic resonance imaging (MRI) and pelvic organ prolapse quantification (POPQ) system in the evaluation of female pelvic organ prolapse. Design: Prospective study. Setting: TantaUniversityHospital. Patients: The study was carried out on 60 patients having clinical manifestations suggesting pelvic floor weakness. Intervention: All the patients were subjected to history taking, physical examination including POPQ, and pelvic MRI (static and dynamic) examination. Outcome measures: Quantitative measurements of genital prolapse. Results: All patients showed loss of the normal position of the perineal plate indicating generalized weakness of the whole pelvic floor muscles. Dynamic MRI exhibited pelvic floor abnormalities in 46 patients who did not show any abnormalities on the static images. Conclusion: Dynamic MRI is a helpful tool in defining the nature and quantitative measurements of pelvic organ prolapse. Also, MRI has and advantage over POPQ system in diagnosing lateral prolapse.