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Search Results: 1 - 10 of 150671 matches for " Mads H Rasmussen "
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Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs
Steffen G Jensen, Philippe Lamy, Mads H Rasmussen, Marie S Ostenfeld, Lars Dyrskj?t, Torben F ?rntoft, Claus L Andersen
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-435
Abstract: Using synthetic miRNA samples and plasma RNA samples spiked with different ratios of 174 synthetic miRNAs we assessed the performance characteristics reproducibility, recovery, specificity, sensitivity and linearity. It was found that while the qRT-PCR based platforms were sufficiently sensitive to reproducibly detect miRNAs at the abundance levels found in human plasma, the array based platform was not. At high miRNA levels both qRT-PCR based platforms performed well in terms of specificity, reproducibility and recovery. At low miRNA levels, as in plasma, the miRCURY platform showed better sensitivity and linearity than the TaqMan platform.For profiling clinical samples with low miRNA abundance, such as plasma samples, the miRCURY platform with its better sensitivity and linearity would probably be superior.microRNAs (miRNAs) are short 20-23 nucleotide long non-coding RNAs that are widely distributed in almost all eukaryotic organisms. They have multiple functions however the main function is believed to be post transcriptional regulation of protein levels [1,2]. While miRNAs are often abundant in tissues, the amount found circulating in body fluids such as plasma and serum is often limited. It has been reported that the total RNA level in plasma is in the range 6-300 ng/ml [3,4] and that the miRNA fraction constitutes only a few percent of this [5]. The mechanisms regulating secretion of miRNA into circulation is still unclear. Reports have shown that while endogenous miRNAs appear stable in plasma/serum exogenous miRNAs are not, and as a result of this it has been suggested that endogenous circulating miRNAs are either encapsulated in microvesicles or bound to RNA-binding proteins in complexes, e.g. Ago2 and NPM1, protecting them from degradation [6-8]. Detailed knowledge of the biological function of circulating miRNA does not exist, however it has been shown that vesicular miRNAs can be transferred from cell to cell and influence the behavior of the recipient c
Standardised assessment of personality – a study of validity and reliability in substance abusers
Morten Hesse, Joachim Rasmussen, Mads Pedersen
BMC Psychiatry , 2008, DOI: 10.1186/1471-244x-8-7
Abstract: We assessed the psychometric properties of the 8-item Standardised Assessment of Personality – Abbreviated Scale (SAPAS) in a sample of 58 methadone maintenance patients.Internal consistency was modest, but similar to the original value (alpha = 0.62), and test-retest correlation at four months follow-up was moderately encouraging for a short instrument such as this (n = 31, test retest intraclass correlation = 0.58), and change at the mean level was minimal, but marginally significant (from an average of 3.3 to 3.8, p = 0.06). Analyses of nurse ratings of patients' behaviour at the clinic showed that SAPAS was significantly correlated with nurse ratings of externalizing behaviour (r = 0.42, p = 0.001), and Global Assessment of Functioning (r = -0.36, p = 0.006), but unrelated to intoxication (r = 0.02, NS), or withdrawal (r = 0.20, NS).There is evidence that the SAPAS is a modestly valid and relatively reliable brief screening measure of personality disorders in patients with ongoing substance abuse undergoing methadone maintenance. It can be used in situations where limited resources are available, and researchers or others wish to get an impression of the degree of personality pathology in a clinical population, as well as for screening purposes.Personality disorders are among the most common co-morbidities among patients with substance use disorders [1,2]. Personality disorders complicate treatment in a range of ways, elicit negative emotional reactions in clinicians [3], and are associated with worse outcome in treatment for substance use disorders [4-7]. Evidence is emerging that substance abuse treatment is more effective, if it addresses personality related issues, including personality disorders [8-10].However, identifying patients with co-morbid personality disorders remain a challenge for substance abuse treatment services. Self-report inventories tend to diagnose nearly all substance abusers with personality disorders [11], and often traits improve rapid
Plasma osteoprotegerin is related to carotid and peripheral arterial disease, but not to myocardial ischemia in type 2 diabetes mellitus
Mikael K Poulsen, Mads Nybo, Jordi Dahl, Susanne Hosbond, Tina S Poulsen, Allan Johansen, Poul F H?ilund-Carlsen, Henning Beck-Nielsen, Lars M Rasmussen, Jan E Henriksen
Cardiovascular Diabetology , 2011, DOI: 10.1186/1475-2840-10-76
Abstract: Type 2 diabetes patients without known CVD referred consecutively to a diabetes clinic for the first time (n = 305, aged: 58.6 ± 11.3 years, diabetes duration: 4.5 ± 5.3 years) were screened for carotid arterial disease, peripheral arterial disease, and myocardial ischemia by means of carotid artery ultrasonography, peripheral ankle and toe systolic blood pressure measurements, and myocardial perfusion scintigraphy (MPS). In addition, plasma OPG concentrations and other CVD-related markers were measured.The prevalence of carotid arterial disease, peripheral arterial disease, and myocardial ischemia was 42%, 15%, and 30%, respectively. Plasma OPG was significantly increased in patients with carotid and peripheral arterial disease compared to patients without (p < 0.001, respectively), however, this was not the case for patients with myocardial ischemia versus those without (p = 0.71). When adjusted for age, HbA1c and U-albumin creatinine ratio in a multivariate logistic regression analysis, plasma OPG remained strongly associated with carotid arterial disease (adjusted OR: 2.12; 95% CI: 1.22-3.67; p = 0.008), but not with peripheral arterial disease or myocardial ischemia.Increased plasma OPG concentration is associated with carotid and peripheral arterial disease in patients with type 2 diabetes, whereas no relation is observed with respect to myocardial ischemia on MPS. The reason for this discrepancy is unknown.at http://www.clinicaltrial.gov webcite: NCT00298844Cardiovascular disease (CVD) is the leading cause of death in type 2 diabetes mellitus patients [1,2], as many suffer from coronary heart disease, cerebrovascular disease, and/or peripheral arterial disease (PAD) [3-5]. Even, asymptomatic patients with type 2 diabetes display subtle CVD [6-8], and therefore it has been debated if screening for unknown CVD should be performed in these patients [8].Osteoprotegerin (OPG) is a soluble member of the TNF-receptor superfamily and act as decoy receptor for both th
Vasopressin-dependent flank marking in golden hamsters is suppressed by drugs used in the treatment of obsessive-compulsive disorder
Craig F Ferris, Mads F Rasmussen, Tara Messenger, Gary Koppel
BMC Neuroscience , 2001, DOI: 10.1186/1471-2202-2-10
Abstract: Male golden hamsters were treated daily for two weeks with either vehicle, fluoxetine, clomipramine, or desipramine (an ineffective drug), before being tested for arginine vasopressin-induced flank marking and grooming. Flank marking was significantly inhibited in animals treated with fluoxetine or clomipramine but unaffected by treatment with desipramine. Grooming behavior was not affected by any treatment.These data suggest that arginine vasopressin-induced flank marking may serve as an animal model for screening drugs used in the control of Obsessive Compulsive Disorder.In golden hamsters, microinjections of arginine vasopressin (AVP) into the anterior hypothalamus trigger grooming and flank marking, a stereotyped scent marking behavior [1,2]. Within a minute after AVP microinjections, hamsters start to lick and comb sebaceous glands situated on their dorsolateral flanks. Bouts of flank gland grooming may be so intense that the flanks are left matted and soaked in saliva. Flank marking behavior is observed soon after the onset of grooming, and usually within two minutes of the microinjection. Flank marking is a scent marking behavior involved in olfactory communication [3], and is characterized by arching the back and rubbing the flank glands vigorously against objects in the environment. The concentrations of AVP required to induce flank marking are low (ranging from 0.1 to 100 μM) [4,5] and the volumes microinjected in the anterior hypothalamus are small (ranging from 20 to 100 nl). The activation of flank marking is dose-dependent, and specific to AVP, as microinjections of other neuropeptides, excitatory amino acids, and catecholamines do not elicit the behavior [1,2,6]. Flank marking is also specific to AVP V1 receptors, as the behavior is selectively inhibited by V 1 receptor antagonists and activated by V1 receptor agonists [4,5].The behavioral effects of AVP are not limited to flank marking in hamsters. Vasopressin is a neurotransmitter released centrally
Erythroderma and its causes with emphasis on idiopathic erythroderma
Mads Rasmussen,Mette Deleuran,Torben Steiniche,Kristian Thestrup-Pedersen
Clinical Dermatology , 2006,
Abstract: Objective: The aimof the presentstudy was to determine the causeof erythroderma in patients referred to ourdepartment with thisdiagnosisfrom 1991 to 2001 inclusivei.e. an 11-years period. A totalof 149 patients wereincluded. We found 35 patients sufferingfrom idiopathic erythroderma, in this papercalled "the Red Man Syndrome". Material and methods: The design was a retrospective follow up based on clinical records. Results: Of all 149 patients 24% had erythroderma of unknown aetiology and without previous skin disease, 24% had erythroderma due to psoriasis, 17% due to atopic dermatitis and 35% had other causes for their erythroderma. In erythroderma of unknown aetiology there was a very high predominance of males with a male/female ratio on 6.0 in contrast to the rest of the patients, who had a male/female ratio around 1.5. Most of the patients with unknown erythroderma (25 of 35) had or developed pleomorphic infiltration in the skin during the observation period. Three progressed to mycosis fungoides and two developed Sezarys syndrome which is 5 of 35 (14%). Seventeen (47%) went into full remission, but 15 (42%) continued to sufferfrom the condition (median observation 27 months). Conclusions: We have confirmed ourfirstdescription of the "Red Man Syndrome", which is a rare condition. We speculate thatthese patients have pre-Sezary's syndrome orerythrodermic cutaneousT-cell lymphoma, not otherwise specified. It is importantto perform repeated biopsies over time and to considerthe cardiological state of the patients due to the increased skin blood-flow.
You Need to Know More to Understand My Scoring on the Survey: Free-Text Comments as Part of a PROM-Survey of Men with Prostate Cancer  [PDF]
Senada Hajdarevic, Birgit H. Rasmussen, Per Fransson
Open Journal of Nursing (OJN) , 2016, DOI: 10.4236/ojn.2016.65038
Abstract: Prostate cancer and its treatment have long-term implications for men’s lives. We aimed to describe the content, extent, and frequency of written comments to the open-ended question, “Further comments?” in the patient-reported outcome measures questionnaire. During the study period, 897 men participated; 372 wrote 747 free-text comments in the questionnaire. These comments were analysed using qualitative content analysis and were grouped into four categories: 1) prostate cancer’s influence on health; 2) clarifications of answers to the survey; 3) descriptions of well-being despite the cancer; and 4) experiences of care and the need for contact with health care. The distribution of the comments shifted over time. The open-ended question not only allowed the participants to explain their other responses and describe important aspects of their lives during and after treatment, something not normally covered by a questionnaire, but it also indicated their experiences of health care services along the patients’ PC-trajectory. This further raises the issue of including an open-ended item in a forced-choice survey into the ethical realm to ensure that proper care is taken of participants’ answers and thoughts.
Probabilistic Output Analysis by Program Manipulation
Mads Rosendahl,Maja H. Kirkeby
Computer Science , 2015, DOI: 10.4204/EPTCS.194.8
Abstract: The aim of a probabilistic output analysis is to derive a probability distribution of possible output values for a program from a probability distribution of its input. We present a method for performing static output analysis, based on program transformation techniques. It generates a probability function as a possibly uncomputable expression in an intermediate language. This program is then analyzed, transformed, and approximated. The result is a closed form expression that computes an over approximation of the output probability distribution for the program. We focus on programs where the possible input follows a known probability distribution. Tests in programs are not assumed to satisfy the Markov property of having fixed branching probabilities independently of previous history.
Tumor-specific usage of alternative transcription start sites in colorectal cancer identified by genome-wide exon array analysis
Kasper Thorsen, Troels Schepeler, Bodil ?ster, Mads H Rasmussen, S?ren Vang, Kai Wang, Kristian Q Hansen, Philippe Lamy, Jakob Pedersen, Asger Eller, Francisco Mansilla, Kirsti Laurila, Carsten Wiuf, S?ren Laurberg, Lars Dyrskj?t, Torben F ?rntoft, Claus L Andersen
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-505
Abstract: By profiling 108 colorectal samples using exon arrays, we identified nine genes (TCF12, OSBPL1A, TRAK1, ANK3, CHEK1, UGP2, LMO7, ACSL5, and SCIN) showing tumor-specific alternative TSS usage in both adenoma and cancer samples relative to normal mucosa. Analysis of independent exon array data sets corroborated these findings. Additionally, we confirmed the observed patterns for selected mRNAs using quantitative real-time reverse-transcription PCR. Interestingly, for some of the genes, the tumor-specific TSS usage was not restricted to colorectal cancer. A comprehensive survey of the nine genes in lung, bladder, liver, prostate, gastric, and brain cancer revealed significantly altered mRNA isoform ratios for CHEK1, OSBPL1A, and TCF12 in a subset of these cancer types.To identify the mechanism responsible for the shift in alternative TSS usage, we antagonized the Wnt-signaling pathway in DLD1 and Ls174T colorectal cancer cell lines, which remarkably led to a shift in the preferred TSS for both OSBPL1A and TRAK1. This indicated a regulatory role of the Wnt pathway in selecting TSS, possibly also involving TP53 and SOX9, as their transcription binding sites were enriched in the promoters of the tumor preferred isoforms together with their mRNA levels being increased in tumor samples.Finally, to evaluate the prognostic impact of the altered TSS usage, immunohistochemistry was used to show deregulation of the total protein levels of both TCF12 and OSBPL1A, corresponding to the mRNA levels observed. Furthermore, the level of nuclear TCF12 had a significant correlation to progression free survival in a cohort of 248 stage II colorectal cancer samples.Alternative TSS usage in colorectal adenoma and cancer samples has been shown for nine genes, and OSBPL1A and TRAK1 were found to be regulated in vitro by Wnt signaling. TCF12 protein expression was upregulated in cancer samples and correlated with progression free survival.Colorectal cancer (CRC) is a leading cause of cancer mo
Functional Screening Identifies miRNAs Influencing Apoptosis and Proliferation in Colorectal Cancer
Lise Lotte Christensen, Anja Holm, Juha Rantala, Olli Kallioniemi, Mads H. Rasmussen, Marie S. Ostenfeld, Frederik Dagnaes-Hansen, Bodil ?ster, Troels Schepeler, Heidi Tobiasen, Kasper Thorsen, Oliver M. Sieber, Peter Gibbs, Philippe Lamy, Torben F. Hansen, Anders Jakobsen, Eva M. Riising, Kristian Helin, Jan Lubinski, Rikke Hagemann-Madsen, S?ren Laurberg, Torben F. ?rntoft, Claus L. Andersen
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096767
Abstract: MicroRNAs (miRNAs) play a critical role in many biological processes and are aberrantly expressed in human cancers. Particular miRNAs function either as tumor suppressors or oncogenes and appear to have diagnostic and prognostic significance. Although numerous miRNAs are dys-regulated in colorectal cancer (CRC) only a small fraction has been characterized functionally. Using high-throughput functional screening and miRNA profiling of clinical samples the present study aims at identifying miRNAs important for the control of cellular growth and/or apoptosis in CRC. The high-throughput functional screening was carried out in six CRC cell lines transfected with a pre-miR library including 319 synthetic human pre-miRs. Phenotypic alterations were evaluated by immunostaining of cleaved cPARP (apoptosis) or MKI67 (proliferation). Additionally, TaqMan Human MicroRNA Array Set v2.0 was used to profile the expression of 667 miRNAs in 14 normal colon mucosa and 46 microsatellite stable stage II CRC patients. Among the miRNAs that induced growth arrest and apoptosis in the CRC cell lines, and at same time were dys-regulated in the clinical samples, miR-375 was selected for further analysis. Independent in vitro analysis of transient and stable transfected CRC cell lines confirmed that miR-375 reduces cell viability through the induction of apoptotic death. We identified YAP1 as a direct miR-375 target in CRC and show that HELLS and NOLC1 are down-stream targets. Knock-down of YAP1 mimicked the phenotype induced by miR-375 over-expression indicating that miR-375 most likely exerts its pro-apoptotic role through YAP1 and its anti-apoptotic down-stream targets BIRC5 and BCL2L1. Finally, in vivo analysis of mouse xenograft tumors showed that miR-375 expression significantly reduced tumor growth. We conclude that the high-throughput screening successfully identified miRNAs that induce apoptosis and/or inhibit proliferation in CRC cells. Finally, combining the functional screening with profiling of CRC tissue samples we identified clinically relevant miRNAs and miRNA targets in CRC.
Analytical and numerical modeling of front propagation and interaction of fronts in nonlinear thermoviscous fluids including dissipation
Anders R. Rasmussen,Mads P. S?rensen,Yuri B. Gaididei,Peter L. Christiansen
Physics , 2008,
Abstract: A wave equation, that governs finite amplitude acoustic disturbances in a thermoviscous Newtonian fluid, and includes nonlinear terms up to second order, is proposed. In contrast to the model known as the Kuznetsov equation, the proposed nonlinear wave equation preserves the Hamiltonian structure of the fundamental fluid dynamical equations in the non-dissipative limit. An exact traveling front solution is obtained from a generalized traveling wave assumption. This solution is, in an overall sense, equivalent to the Taylor shock solution of the Burgers equation. However, in contrast to the Burgers equation, the model equation considered here is capable to describe waves propagating in opposite directions. Owing to the Hamiltonian structure of the proposed model equation, the front solution is in agreement with the classical Rankine-Hugoniot relations. The exact front solution propagates at supersonic speed with respect to the fluid ahead of it, and subsonic speed with respect to the fluid behind it, similarly to the fluid dynamical shock. Linear stability analysis reveals that the front is stable when the acoustic pressure belongs to a critical interval, and is otherwise unstable. These results are verified numerically. Studies of head-on colliding fronts demonstrate that the front propagation speed changes upon collision.
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