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Search Results: 1 - 10 of 634767 matches for " M.B.;Mandarim-de-Lacerda "
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Beneficial effects of exercise training (treadmill) on insulin resistance and nonalcoholic fatty liver disease in high-fat fed C57BL/6 mice
Marques, C.M.M.;Motta, V.F.;Torres, T.S.;Aguila, M.B.;Mandarim-de-Lacerda, C.A.;
Brazilian Journal of Medical and Biological Research , 2010, DOI: 10.1590/S0100-879X2010007500030
Abstract: c57bl/6 mice develop signs and symptoms comparable, in part, to the human metabolic syndrome. the objective of the present study was to evaluate the effects of exercise training on carbohydrate metabolism, lipid profile, visceral adiposity, pancreatic islet alterations, and nonalcoholic fatty liver disease in c57bl/6 mice. animals were fed one of two diets during an 8-week period: standard (sc, n = 12) or very high-fat (hf, n = 24) chow. an exercise training protocol (treadmill) was then established and mice were divided into sc and hf sedentary (sc-sed, hf-sed), exercised groups (sc-ex, hf-ex), or switched from hf to sc (hf/sc-sed and hf/sc-ex). hf/hf-sed mice had the greatest body mass (65% more than sc/sc-sed; p < 0.0001), and exercise reduced it by 23% (p < 0.0001). hepatic enzymes alp (+80%), alt (+100%) and ast (+70%) were higher in hf/hf mice than in matched sc/sc. plasma insulin was higher in both the hf/hf-sed and hf/sc-sed groups than in the matched exercised groups (+85%; p < 0.001). pancreatic islets, adipocytes and liver structure were greatly affected by hf, ultimately resulting in islet β-cell hypertrophy and severe liver steatosis. the hf group had larger islets than the sc/sc group (+220%; p < 0.0001), and exercise significantly reduced liver steatosis and islet size in hf. exercise attenuated all the changes due to hf, and the effects were more pronounced in exercised mice switched from an hf to an sc diet. exercise improved the lipid profile by reducing body weight gain, visceral adiposity, insulin resistance, islet alterations, and fatty liver, contributing to obesity and steatohepatitis control.
Maternal Vitamin D Deficiency Delays Glomerular Maturity in F1 and F2 Offspring
Fernanda A. M. Nascimento, Thais C. Ceciliano, Marcia B. Aguila, Carlos A. Mandarim-de-Lacerda
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041740
Abstract: Background There is a high prevalence of vitamin D insufficiency in women of reproductive age. Methods This work studied the first two generations of offspring (F1 and F2) of Swiss mice from mothers fed one of two diets: SC (standard chow) or VitD- (vitamin D-deficient). Functional and developmental kidney measurements were taken. Results The first two generations of the VitD- group had higher blood pressure at 6 months of age than the offspring of the SC group as well as an increase in renin and AT1r expression. However, at all ages, both F1 and F2 VitD- mice had shorter glomerular diameters, and diet played a significant role in the total variation. Both the F1 and F2 generations of the VitD- group had more immature glomeruli than offspring from the SC group. Immature glomeruli begin to disappear at 10 days, but at this age, F1-VitD- mice had more immature and mature glomeruli than F1-SC mice. At 6 months of age, F1-VitD- mice exhibited more glomeruli, while F2-VitD- mice exhibited the same number of glomeruli as F2-SC mice, but fewer glomeruli compared to the F1-VitD group. Both diet and generation account for the total variation in the number of glomeruli. Decreases in urine output and podocin expression and increases in urea and creatinine in the urine were observed in F1 offspring. Conclusion These findings demonstrate that maternal vitamin D deficiency accompanies changes in the renal expression of important factors that may retard the maturation of glomeruli by extending the period of nephrogenesis.
Hepatic Adverse Effects of Fructose Consumption Independent of Overweight/Obesity
Alini Schultz,Debora Neil,Marcia B. Aguila,Carlos A. Mandarim-de-Lacerda
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141121873
Abstract: The chronic intake of fructose has been linked to insulin resistance, obesity, dyslipidemia and nonalcoholic fatty liver disease (NAFLD), which in turn, may progress to nonalcoholic steatohepatitis (NASH). We aimed to evaluate the magnitude of the effects of the chronic consumption of high-fructose (HFr) and high fat (HF) alone or combined. Four groups of male mice were fed different diets for 16 weeks: standard chow (9% fat: SC), HF diet (42% fat), HFr diet (34% fructose) and HF/HFr diet (42% fat, 34% fructose). The food intake was not different among the groups, and the body mass was not greater in the HFr group than in the SC group. The homeostasis model assessment for insulin resistance (HOMA-IR), as well as plasmatic total cholesterol and triglycerides were greater in the groups HF, HFr, and HF/HFr group than in the SC group. We observed in the groups HF, HFr and HF/HFr, compared to the group SC, nonalcoholic fatty liver disease (NAFLD) with a predominance of lipogenesis mediated by SREBP-1c and PPAR-γ, and a reduction of the oxidation mediated by PPAR-α. We also observed an increase in gluconeogenesis mediated by the GLUT-2 and the PEPCK. Importantly, we identified areas of necroinflammation indicating a transition from NAFLD to nonalcoholic steatohepatitis in the HFr and HF/HFr groups. This study is relevant in demonstrating that fructose consumption, even in the absence of obesity, causes serious and deleterious changes in the liver with the presence of the dyslipidemia, insulin resistance (IR), and NAFLD with areas of necroinflammation. These conditions are associated with a poor prognosis.
Papel da óxido nítrico sintase na etiopatogenia da estenose hipertrófica do piloro na infancia
Barbosa, Irnak M.;Ferrante, Saulo M.R.;Mandarim-de-Lacerda, Carlos A.;
Jornal de Pediatria , 2001, DOI: 10.1590/S0021-75572001000400013
Abstract: objective: to experimentally reproduce, in rats, the findings corresponding to the histopathology of infantile hypertrophic pyloric stenosis (ihsp), using nitric oxide synthase (nos) inhibitor (l-name). methods: l-name was administered to pregnant rats (l-name group), from the 14th gestational day on in order to reproduce the model of nos inhibition in the production of ihsp. this group was then compared to control animals. after birth, all the animals in the l-name group were maintained under nos inhibition until the 42nd day of life, when they were sacrificed. the control animals, which did not receive any kind of drug, were also sacrificed on the 42nd day of life. the animals and their internal organs were analyzed and weighed. the pyloric region was technically prepared and observed through light microscopy. results: the l-name group presented lower body and intestinal weight and higher gastric weight than the control group. light microscopy revealed hypertrophy of the circular smooth muscle layer of the pyloric muscle in l-name animals. conclusions: this work reproduced an experimental model of an ihsp study, confirming the effect of nos blockade on the pyloric musculature. conclusions: this work reproduced an experimental model of an ihsp study, confirming the effect of nos blockade on the pyloricmusculature.
Myocardial repair with long-term and low-dose administration of a nitric oxide synthesis inhibitor. Myofibroblasts, type III collagen and fibronectin
Pessanha, M?nica Gomes;Hahn, Myriam Dumas;Mandarim-de-Lacerda, Carlos Alberto;
Arquivos Brasileiros de Cardiologia , 1999, DOI: 10.1590/S0066-782X1999000700008
Abstract: objective: to study the healing process of the myocardium in hypertensive rats undergoing inhibition of nitric oxide synthesis. methods: two groups of animals were studied: one received l-name, 12mg/kg/day, and the other was a control group. the presence of type iii collagen, fibronectin, and a-smooth muscle actin-positive cells was assessed by immunohistochemistry. results: fibronectin was seen in both early and late lesions, while type iii collagen was seen mainly in areas of incomplete healing, situated among myocytes and around the intramyocardial branches of the coronary arteries. areas representing early and late lesions showed a population of spindle-shaped cells. immunohistochemistry showed that these cells were positive for a-smooth muscle actin. conclusion: in the myocardium of hypertensive rats, the a-smooth muscle actin-positive cells are related to the accumulation of type iii collagen and fibronectin in the areas of myocardial damage.
Estereologia do miocárdio de ratos jovens e idosos
águila, Márcia Barbosa;Mandarim-de-Lacerda, Carlos Alberto;Apfel, Mara Ibis Rodrigues;
Arquivos Brasileiros de Cardiologia , 1998, DOI: 10.1590/S0066-782X1998000200007
Abstract: purpose: to determine the myocardial quantitative changes comparing young and aged animals by using the stereology. methods: thirty rat hearts were studied (15 rats aged 3 months and 15 other rats aging 15 months). the hearts were removed, weighed, fixed in 10% buffered formaldehyde solution, embedded in paraffin, cut in 7μm thick slices and stained with he and picro-sirius stains. in each group we counted 15 random microscopic fields. the following parameters were studied: vv(myocyte) and vv(interstitium)(%) (the volume densities of the cardiac myocyte and interstitium, determined by the point-counting method), and nv(myocyte) (1/mm3) (the numerical density of the cardiac myocytes, determined with the disector method). the total number of myocytes (n[myocyte]) and the mean volume of the myocytes (v[myocytes]) were also determined. the differences were tested by the mann-whitney test.results: cardiac weight increased from 1.1 to 1.7g, the vv(myocyte) decreased from 76.7 to 72.2%, the vv(interstitium) increased from 23.3 to 27.8%. the nv(myocyte) and the n(myocyte) decreased from 14.76x104 to 6.19x104/mm3 and 15.64x104 to 10.72x104 myocytes, respectively. simultaneously, the v(myocyte) increased from 5.42x103 to 13.26x103mm3. these differences were statistically significant (p<0.05). conclusion: myocardial changes, comparing young rats with aged ones suggest loss of myocytes (increased apoptosis?) with simultaneous myocyte hypertrophy.
COMPARISON BETWEEN COMPUTED TOMOGRAPHY AND ULTRASONOGRAPHY IN THE CEREBRAL VENTRICLES MORPHOMETRY
Boasquevisque,Edson M.; Mandarim-de-Lacerda,Carlos A.; Cerri,Giovani G.;
Revista chilena de anatomía , 2000, DOI: 10.4067/S0716-98682000000100002
Abstract: encephalic ventricles were evaluated by ct and us in normal subjects and in patients suffering of meningitis. the biometry and statistical analyses were performed observing the ventricular index (vi%), and the diameters of the frontal and temporal horns of the lateral ventricle, and the diameters of the iii and iv ventricles. comparing the two diagnostic methods the coefficient of correlation was high for the lateral ventricle diameter (r=0.94) and for the vi (0.85). the sensitivity of the us was better than the ct in the observation of the temporal horn diameter. the us, because it high accuracy, it low cost and the possibility to be a portable equipment, is a useful method to investigate cerebral ventricles in infants
COMPARISON BETWEEN COMPUTED TOMOGRAPHY AND ULTRASONOGRAPHY IN THE CEREBRAL VENTRICLES MORPHOMETRY COMPARACIóN ENTRE TOMOGRAFíA COMPUTARIZADA Y ULTRA-SONOGRAFíA EN LA MORFOMETRíA DE LOS VENTRíCULOS CEREBRALES
Edson M. Boasquevisque,Carlos A. Mandarim-de-Lacerda,Giovani G. Cerri
Revista chilena de anatomía , 2000,
Abstract: Encephalic ventricles were evaluated by CT and US in normal subjects and in patients suffering of meningitis. The biometry and statistical analyses were performed observing the ventricular index (VI%), and the diameters of the frontal and temporal horns of the lateral ventricle, and the diameters of the III and IV ventricles. Comparing the two diagnostic methods the coefficient of correlation was high for the lateral ventricle diameter (r=0.94) and for the VI (0.85). The sensitivity of the US was better than the CT in the observation of the temporal horn diameter. The US, because it high accuracy, it low cost and the possibility to be a portable equipment, is a useful method to investigate cerebral ventricles in infants Ventrículos encefálicos fueron evaluados por CT y US en sujetos normales y en pacientes afectados de meningitis. La biometría y el análisis estadístico fueron ejecutados observando el índex ventricular (VI%), los diámetros de los cuernos frontal y temporal del ventrículo lateral, y los diámetros de los ventrículos III y IV. Comparando los dos métodos diagnósticos, el coeficiente de correlación fue alto para el diámetro del ventrículo lateral (r=0.94) y para el VI (0.85). La sensibilidad de la US fue mejor que para la CT en la observación del diámetro del cuerno temporal. La US, por ser de alta precisión, de bajo costo y por emplear un equipamiento transportable, resulta un método útil para la investigación de los ventrículos cerebrales
Myocardial repair with long-term and low-dose administration of a nitric oxide synthesis inhibitor. Myofibroblasts, type III collagen and fibronectin
Pessanha M?nica Gomes,Hahn Myriam Dumas,Mandarim-de-Lacerda Carlos Alberto
Arquivos Brasileiros de Cardiologia , 1999,
Abstract: OBJECTIVE: To study the healing process of the myocardium in hypertensive rats undergoing inhibition of nitric oxide synthesis. METHODS: Two groups of animals were studied: one received L-NAME, 12mg/kg/day, and the other was a control group. The presence of type III collagen, fibronectin, and alpha-smooth muscle actin-positive cells was assessed by immunohistochemistry. RESULTS: Fibronectin was seen in both early and late lesions, while type III collagen was seen mainly in areas of incomplete healing, situated among myocytes and around the intramyocardial branches of the coronary arteries. Areas representing early and late lesions showed a population of spindle-shaped cells. Immunohistochemistry showed that these cells were positive for alpha-smooth muscle actin. CONCLUSION: In the myocardium of hypertensive rats, the alpha-smooth muscle actin-positive cells are related to the accumulation of type III collagen and fibronectin in the areas of myocardial damage.
Estereologia do miocárdio de ratos jovens e idosos
águila Márcia Barbosa,Mandarim-de-Lacerda Carlos Alberto,Apfel Mara Ibis Rodrigues
Arquivos Brasileiros de Cardiologia , 1998,
Abstract: OBJETIVO: Comparar as diferen as quantitativas na composi o e estrutura do miocárdio de ratos jovens e idosos utilizando a estereologia. MéTODOS: Foram estudados 30 ratos machos da ra a Wistar (15 animais jovens de 3 meses de idade e 15 animais idosos de 15 meses). Os cora es foram retirados, pesados, perfundidos com solu o de Bouin e posteriormente fixados em formol tamponado a 10% por 24h, processados por técnica histológica, incluídos em parafina, seccionados e corados pelo HE e picro-sirius. Foram analisados 15 campos aleatórios por grupo e foram determinados os seguintes parametros estereológicos: densidade de volume do miócito (Vv[miócito]) e densidade de volume do interstício cardíaco (Vv[interstício]) e densidade numérica dos miócitos (Nv[miócito]) (1/mm3), estimados pelo método disector. O número total de miócitos (N[miócitos]) e a média do volume do miócito (Vol[miócito]) dos cora es dos dois grupos também foram determinados. As diferen as estatísticas entre os animais jovens e idosos foram testadas pelo teste n o paramétrico de Mann-Whitney. RESULTADOS: Comparando os animais dos grupos jovem e idoso temos, respectivamente, os seguintes dados: o peso cardíaco aumentou de 1,1 para 1,7g, o Vv(miócito) diminuiu de 76,7 para 72,2%, Vv(interstício) aumentou de 23,3 para 27,8%, enquanto o Nv(miócito) diminuiu de 14,76x10(4) para 6,19x10(4)/mm3. O Vol(miócito) aumentou de 5,42x103 para 13,26x103mm3 e o N(miócito) diminuiu de 15,64x10(4) para 10,72x10(4) miócitos. Estas diferen as foram estatisticamente significativas (p<0,05). CONCLUS O: Os resultados obtidos mostram que no envelhecimento ocorre perda de miócitos cardíacos e hipertrofia das células remanescentes.
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