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Search Results: 1 - 10 of 637388 matches for " M. S. QURESHI1 AND U. SADIQUE1 "
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COMPARATIVE EFFICIENCY OF SOME INDIRECT DIAGNOSTIC TESTS FOR THE DETECTION OF SUB-CLINICAL MASTITIS IN COWS AND BUFFALOES
M. IQBAL, M. AMJED1, M. A. KHAN, M. S. QURESHI1 AND U. SADIQUE1
Pakistan Veterinary Journal , 2006,
Abstract: The present study was undertaken to compare five laboratory diagnostic tests for sub-clinical mastitis in cattle and buffaloes and to compute cost, time taken by each test and its ranking for availability, adoptability, interpretability and sensitivity. There were 352 cases with each test type viz. California Mastitis Test (CMT), White Side Test (WST), White Side + Dye (WSTD), Surf Test and Surf + Dye, and 880 cases with each species type (cattle and buffaloes). Result scores (1760 ) for sub-clinical mastitis in each category of negative, trace, single positive, double positive and triple positive by species, and laboratory tests, were analyzed using nonparametric tests. Chi-square statistics showed that CMT was equally effective at both locations (farm vs. laboratory). Correlation further suggested that the association was highly significant. Moreover, cases in category of negative, trace and single positive strongly differed (P<0.05) amongst the laboratory tests. But differences were found vague in double and triple positive categories. However, Chi-square statistics of overall sub-clinical mastitis cases showed that the laboratory tests were significantly different for detecting various categories of sub-clinical mastitis of a cross-tabulation. The present findings uncovered stronger (P<0.05) positive correlation of both the WST and WSTD with CMT, while of Surf Test and Surf + Dye with CMT. Results also suggested that WSTD and Surf + Dye were equally associated with CMT in strength and direction as their counterpart tests WST and Surf Test with CMT and thus its response in efficacy to added dye was not distinct. Species effect on result scores was found negligible (P>0.05). The study further suggested that CMT was the most sensitive test, followed by WST/WSTD and Surf/Surf + Dye. Although, the five tests showed slight discrepancy in the trace category reaction, a strong relationship of Surf Test to CMT, its low cost, easy availability and readily adoptable qualities should spur the relevant authorities to recommend the use of Surf test as a routine practice in dairy farming and add this test in the curriculum of diploma and degree programmes.
Involvement of Mycoplasma synoviae in Respiratory Distress Cases of Broilers
S. Ehtisham-ul-Haque*, S. U. Rahman, M. Siddique and A. S. Qureshi1
Pakistan Veterinary Journal , 2011,
Abstract: Mycoplasma synoviae (MS) is an important pathogen of poultry worldwide, causing respiratory tract infection and infectious synovitis in chickens and turkeys. The study was designed to detect M. synoviae through serology, culture isolation and polymerase chain reaction (PCR) assay to document the involvement of MS infection in respiratory distress cases of broiler birds. The validated PCR assay amplifying the conserved gene region of 16SrRNA gene was applied for the detection of M. synoviae from culture as well as in clinical samples. The results indicated that 04 out of total 17 commercial broiler flocks showing respiratory distress signs were found positive with M. synoviae infection indicating 76.57% sero-positivity as, determined with rapid serum agglutination (RSA) test. Out of 85 clinical specimens (collected from sero-positive birds); M. synoviae culture isolation was successfully attained in 36 (42.35%) samples. Whereas, PCR test has detected 84 (98.82%) positive cases. The prevalence of MS in broiler birds was observed maximum as measured through PCR. It is suggested that the true prevalence of MS may best be reflected by combining RSA and PCR test findings.
EFFECTS OF GOSSYPOL ON SEMEN CHARACTERISTICS IN TEDDY MALE GOATS
I.A. Zahid, L.A. Lodhi1, N. Ahmad1, Z.I. Qureshi1, N.U. Rehman1 and M.S. Akhtar2
Pakistan Veterinary Journal , 2003,
Abstract: In the present study, the effects of gossypol on semen characteristics in Teddy male goats were studied. Nine Teddy male goats were randomly divided into three equal groups named A, B and C. Animals in all groups were fed concentrated ration without cottonseed cakes (CSC) at the rate of 3% of their liveweight for a period of 30 days and it was named as pre-treatment period. Just after the completion of this period, animals in group A were fed control ration (without gossypol), those in group B were fed ration which contained unboiled CSC as a source of free and bound gossypol, while animals in group C were given ration containing CSC boiled at 100 C for 1 hour as a source of bound gossypol These experimental rations were fed to animals of respective groups at the rate of 3% of their liveweight for a period of 90 days and it was named as treatment period. Feeding of ration containing gossypol to Teddy male goats did not affect the colour, volume, mass activity, sperm concentration, percentage of dead spermatozoa, liveability and absolute index of liveability of spermatozoa at 37°C. However, it affected significantly (P<0.05) the pH, per cent motility of spermatozoa and percentage of morphologically abnormal spermatozoa. The Teddy male goats fed rations containing a combination of free and bound gossypol showed a significant (P<0.05) increase in the pH and a decrease in motility of spermatozoa which was statistically lower than those fed control diet or diet containing bound gossypol. It was concluded that rations containing a combination of free and bound gossypol (unboiled CSC) or bound gossypol only (boiled CSC) adversely affected the semen quality of Teddy male goats in terms of sperm motility and morphologically abnormal spermatozoa in ejaculates.
SEQUENCING AND SEQUENCE ANALYSIS OF MYOSTATIN GENE IN THE EXON 1 OF THE CAMEL (CAMELUS DROMEDARIUS)
M. G. SHAH, A. S. QURESHI1, M. REISSMANN2 AND H. J. SCHWARTZ3
Pakistan Veterinary Journal , 2006,
Abstract: Myostatin, also called growth differentiation factor-8 (GDF-8), is a member of the mammalian growth transforming family (TGF-beta superfamily), which is expressed specifically in developing an adult skeletal muscle. Muscular hypertrophy allele (mh allele) in the double muscle breeds involved mutation within the myostatin gene. Genomic DNA was isolated from the camel hair using NucleoSpin Tissue kit. Two animals of each of the six breeds namely, Marecha, Dhatti, Larri, Kohi, Sakrai and Cambelpuri were used for sequencing. For PCR amplification of the gene, a primer pair was designed from homolog regions of already published sequences of farm animals from GenBank. Results showed that camel myostatin possessed more than 90% homology with that of cattle, sheep and pig. Camel formed separate cluster from the pig in spite of having high homology (98%) and showed 94% homology with cattle and sheep as reported in literature. Sequence analysis of the PCR amplified part of exon 1 (256 bp) of the camel myostatin was identical among six camel breeds.
SINGLE NUCLEOTIDE POLYMORPHISM IN THE CODING REGION OF MYF5 GENE OF THE CAMEL (CAMELUS DROMEDARIUS)
M. G. SHAH, A. S. QURESHI1, M. REISSMANN2 AND H. J. SCHWARTZ3
Pakistan Veterinary Journal , 2007,
Abstract: The myogenic factors (MYF) 5 and 6 are integral to the initiation and development of skeletal muscles and to the maintenance of their phenotypes. Thus, they are candidate genes for growth and meat quality-related traits. The MYF5 gene is expressed during proliferation of myoblasts and comprises 3 exons: 500, 76 and 191 bp long. Genomic DNA was isolated from the camel hair using NucleoSpin Tissue kit. Two animals of each of the six breeds namely, Marecha, Dhatti, Larri, Kohi, Sakrai and Cambelpuri were used for sequencing. For PCR amplification of the gene, a primer pair was designed from homolog regions of already published sequences of farm animals from GenBank. Results showed that exon 1 comprising of 422 bp of the dromedary MYF5 gene was more homologous (94%) to the cattle than the dog and human. However, phylogram showed that a small number of mutations had been experienced by dromedary camels at their MYF5 gene and was more near to human than other farm animals.
Serum Ovarian Steriod Hormones and Some Minerals Concentration in Pregnant Nili-Ravi Buffaloes with or without Pre-Partum Vaginal Prolapse
M. S. Akhtar*, L. A. Lodhi1, I. Ahmad1, Z. I. Qureshi1 and G. Muhammad2
Pakistan Veterinary Journal , 2012,
Abstract: The present study was designed with the objective to determine the hormones (estradiol, progesterone) and minerals (calcium, phosphorus, magnesium, sodium and potassium) in serum of buffaloes suffering from pre-partum vaginal prolapse. A total of 200 buffaloes were included in this study, half of these were suffering with pre-partum vaginal prolapse (affected group) between 7th to 10th months of gestation while the remaining were normal pregnant buffaloes (healthy group). Blood samples were collected to procure serum from each animal. Serum hormones and minerals were determined by ELISA technique and direct colorimetric method, respectively. The mean serum estradiol and magnesium concentrations were significantly higher (P<0.01) whereas progesterone, calcium and phosphorus concentrations were significantly lower (P<0.01) in buffaloes suffering with pre-partum vaginal prolapse as compared to healthy pregnant buffaloes. It was concluded that the serum hormones (estradiol and progesterone) and minerals (magnesium, calcium and phosphorus) concentrations differ between the vaginal prolapse affected and healthy buffaloes.
Effect of Artificial Feed on Sensory Attributes of Flesh of Indian Major Carps (Labeo rohita, Catla catla and Cirrhinus mrigala) Fed in Monoculture and Polyculture Systems
N. Khan*, N. A. Qureshi1, M. Nasir2, G. W. Vandenberg3, M. S. Mughal, A. Maqbool4, M. A. Jabbar and N. Zikria
Pakistan Veterinary Journal , 2012,
Abstract: The study was designed to assess the impact of artificial feeding on sensory attributes of steamed fish at fingerling stage reared in monoculture while at grow-out stage reared both in mono and polyculture. The fingerlings of Labeo rohita, Catla catla and Cirrhinus mrigala fed on 40% protein feed showed non-significant effect on sensory attributes compared with control. Similar results were obtained in grow-out monoculture when fed on 35% protein. In polyculture the C. mrigala showed significantly (P<0.05) higher scores for color, oiliness and overall acceptability compared to control and other two species. All three species showed significant differences for flavor, and tenderness in polyculture compared to monoculture. The control fish in polyculture were slightly more yellowish resulting in increased hue angle values. It is concluded that the artificial feed can be used to enhance the growth performance at fingerling stage without interfering the overall sensory quality of fish.
Effect of Administration of Withania somnifera on Some Hematological and Immunological Profile of Broiler Chicks
Muhammad Mushtaq*, F. R. Durrani, N. Imtiaz, Umer Sadique1, A. Hafeez, S. Akhtar2 and S. Ahmad3
Pakistan Veterinary Journal , 2012,
Abstract: This study was conducted to assess the performance of broiler chickens based on some hematological and immunological profile, upon inclusion of aqueous extract of Withania somnifera (W. somnifera) in their drinking water. For this purpose, 240 a-day-old broiler chicks were purchased from a local hatchery and divided into four groups i.e. WST-0, WST-I, WST-II and WST-III. Group WST-0 was kept as control, while chicks in group WST-I, WST-II and WST-III were offered in their drinking water with an extract of W. somnifera at 10, 20 and 30g/L for 35 days, starting from day 1 of age. Feed intake and body weight were recorded on regular basis and hematological and immunological profile of the birds was analyzed at the end of experiment. Administration of W. somnifera extract resulted in significantly higher feed intake and body weight of birds in all treated groups. An increase in the values of Hb, PCV and TLC was noted in the treatment groups. A non significant difference was noted in the counts of neutrophils, lymphocytes, eosinophils and monocytes in all treatment groups as compared to control. Antibodies titers against IB and IBD were significantly higher in all treatment groups as compare to the values shown by the chicks in control group. Findings of this study indicated that administration of W. somnifera extract to broiler chicks improve their feed intake, body weight gain, hematological profile and immunological status.
EFFECTIVENESS OF SUPPLEMENTATION OF VITAMIN C IN BROILER FEEDS IN HOT SEASON
Abdul Qayum Raja and Aijazuddin Qureshi1
Pakistan Veterinary Journal , 2000,
Abstract: no abstract
STUDY OF SOME PRODUCTION TRAITS IN SAHIWAL COWS AND THEIR CALVES AFTER IMMUNOSTIMULATION OF PREGNANT DAMS DURING LATE GESTATION
A. Sattar, L. A. Lodhi1, I. Ahmad, Z. I. Qureshi1 and N. Ahmad1
Pakistan Veterinary Journal , 2003,
Abstract: Thirty two Sahiwal cows (Bos indicus) in the last trimester of pregnancy were used to evaluate the effect of immunostimulation on some production traits in the control and treated dams and calves born to them. The cows were divided into four equal groups. Group-I served as untreated control while groups-II, III and IV were treated with levamisole hydrochloride (0.5 mg/Kg body weight orally), vitamin E-selenium (10 ml intramuscularly per cow) and BCG (Bacille Calmette Guerin) vaccine (0.5 ml subcutaneously per cow), respectively. These immunostimulants were given twice with one week interval at about 60 days prior to expected date of calving. Some production traits, i.e., milk yield and lactation length of the control and treated dams while birth weight, weaning weight and growth rate upto weaning of calves born to these dams were determined. Milk yield in Sahiwal cows was the lowest (1032.63 litres) in control group and highest (1341.63 litres) in levamisole treated group while lactation length in both these groups was 224.63 and 266.00 days, respectively. The birth weight was the lowest (21.38 kg) in the calves born to control group of cows while weaning weight and growth rate were the lowest (48.33 kg and 0.302 kg/day) in the calves born to BCG treated group of cows. The highest values of birth weight, weaning weight and growth rate (24.38 kg, 53.86 kg and 0.330 kg/day) were observed in the calves born to levamisole treated group of Sahiwal cows. The differences between all the groups for all the production traits recorded were statistically non-significant except one trait, i.e., growth rate which was significantly higher in the calves born to levamisole treated cows as compared to those born to BCG treated dams.
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