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Search Results: 1 - 10 of 410409 matches for " M?sar Lemos "
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In vitro behavior of Mycoplasmagallisepticum live-type nosode
M???′sar Lemos,Elmiro Rosendo do Nascimento,Maria Lucia Barreto,Virginia Leo de Almeida Pereira
International Journal of High Dilution Research , 2011,
Abstract: As a step of a doctoral research project, in this study a live-type nosode was prepared from microorganism Mycoplasmagallisepticum strain R (ATCC 93-08/19610) according to Costa model and the rules by Brazilian Homeopathic Pharmacopoeia. Live nosode was tested in vitro to assess safety when used to immunize domestic fowl (Gallus gallus) against infection by this microorganism and to investigate its behavior under laboratory conditions. M. gallisepticum was not shown to grow in fluid (broth) and solid (plate) modified Frey medium with dilutions 11d, 12d, 20d and 30d. Inhibition halos about 2.0 mm were observed around paper disks impregnated with live-type nosode in microorganism-sown Petri dishes, whereas disks impregnated with conventional antibiotic oxytetracycline exhibited 8.0 mm inhibition halos. Protein assessment by Folin-Lowry method showed protein absence in dilutions 12d and 30d and neither microbial DNA traces were found in PCR assay in dilutions 12d, 20d and 30d.
Quality of Semi-Prepared Products from Rainbow Trout Waste (Onchorynchus mykiss) by Using Different Technological Strategics  [PDF]
Karoline Ribeiro Palmeira, Eliane Teixeira Mársico, Laís Doro, Msar Lemos, Claudia Emília Teixeira, Vania Margaret Flosi Paschoalin, Maria Lúcia Guerra Monteiro, Carlos Adam Conte Júnior
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.56067

The consumption of freshwater fish and fish products has gradually grown worldwide over the last decades, generating a proportional waste increase. The objective of the present study was to assess the chemical and bacteriological quality of restructured fish product, meatball-type, prepared with rainbow trout (Onchorynchus mykiss) waste added of 1% transglutaminase (MTG), 4% textured soy protein (TSP) and replacing part of the sodium chloride with potassium chloride (75%/25%) as described below: T1—starch addition (control); T2—MTG addition (1%); T3—soy protein addition (4%); T4—soy protein addition (4%) and MTG addition (1%); T5—soy protein addition (4%), MTG addition (1%) and partial replacement of salt (75% NaCl/25% KCl). Total aerobic mesophilic bacteria (TAMB), 2-thiobarbituric acid reactive substances (TBARS), pH determination and quantification of biogenic amines were performed on the day after manufacturing (P0) and after 60 days of storage (P1) at -25℃ ± 2℃. The results showed that there was no significant difference (p < 0.05) of microbiological quality, TBARS and pH after storage. T4 presented the lowest total biogenic amine content (256.84 mg/kg) whereas T3 and T5 had the highest value (791.36 and 707.19 mg/kg, respectively) in this parameter. Putrescine was the biogenic amine that presented the highest concentration (504.00 mg/kg) in T3 and cadaverine that presented the smallest concentration (0.36 mg/kg) in T4. The use of technological strategies for developing new products with non-commercial fillets kept the most standards, having changes only in some biogenic amines.

Fatores de risco associados à mastite bovina causada por Prototheca zopfii
Yamamura, Aline Artioli Machado;Müller, Ernst Eckhardt;Freire, Roberta Lemos;Freitas, Júlio César de;Pretto-Giordano, Lucienne Garcia;Toledo, Roberta dos Santos;Ribeiro, Márcio Garcia;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000300025
Abstract: this research had as objective the study of risk factors associated with bovine mastitis caused by prototheca zopfii. thirteen dairy herds in paraná and s?o paulo states were analyzed and selected according to the following criteria: previous confirmation of prototheca spp. mastitis cases, screening of prototheca spp. in bulk tanks and milk cans, and herds with somatic cells count over 5x105cel ml-1. the samples collected consisted of: milk, water, soil, manure and swabs of teat cup rubbers. prototheca spp. was isolated from mammary quarters with clinical and subclinical mastitis of milk samples in one herd and from the environment and cows in four herds. out of 383 cows examined, prototheca spp. was isolated in 20 (5.2%) cows with mastitis, and 18 of them were characterized as p. zopfii. in four herds when prototheca spp. was identified from mammary quarters and environment the agent was isolated from the following samples: water in the waterers, puddled water in the stalls and the milking parlour, supply, sewage, cow pen and pasture soil, teat cup rubbers and manure from calves and swines. the risk factors associated with p. zopfii mastitis consisted of: pasture system, pasture and silage feeding, use of milking machine in stalls, cow pen without fresh feed after milking, raising of swines near bovine housing, existence of dogs, cats and rodents, absence of teats hygienization with water, use of pre-immersion devices with return and without change of antiseptic, calves fed with milk of clinical mastitis cases and the holstein breed.
Molecular characterization of bacterial populations of different soils
Pereira, Rodrigo Matheus;Silveira, érico Leandro da;Scaquitto, Denilson César;Pedrinho, Eliamar Aparecida Nascimbém;Val-Moraes, Silvana Pompéia;Wickert, Ester;Carareto-Alves, Lúcia Maria;Lemos, Eliana Gertrudes de Macedo;
Brazilian Journal of Microbiology , 2006, DOI: 10.1590/S1517-83822006000400007
Abstract: until recently, few studies were carried out in brazil about diversity of bacterial soil communities. aiming to characterize the bacterial population in the soil through 16s rrna analysis, two types of soil have been analyzed: one of them characterized by intensive use where tomato, beans and corn were cultivated (cs); the other analyzed soil was under forest (fs), unchanged by man; both located in guaíra, s?o paulo state, brazil. using specific primers, 16s rrna genes from metagenomic dna in both soils were amplified by pcr, amplicons were cloned and 139 clones from two libraries were partially sequenced. the use of 16s rrna analysis allowed identification of several bacterial populations in the soil belonging to the following phyla: acidobacteria, actinobacteria, bacteroidetes, firmicutes, proteobacteria verrucomicrobia in addition to the others that were not classified, beyond archaea domain. differences between fs and cs libraries were observed in size phyla. a larger number of phyla and, consequently, a greater bacterial diversity were found in the under-forest soil. these data were confirmed by the analyses of genetic diversity that have been carried out. the characterization of bacterial communities of soil has made its contribution by providing facts for further studies on the dynamics of bacterial populations in different soil conditions in brazil.
Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis
Silveira, érico Leandro da;Pereira, Rodrigo Matheus;Scaquitto, Denilson César;Pedrinho, Eliamar Aparecida Nascimbém;Val-Moraes, Silvana Pómpeia;Wickert, Ester;Carareto-Alves, Lúcia Maria;Lemos, Eliana Gertrudes de Macedo;
Pesquisa Agropecuária Brasileira , 2006, DOI: 10.1590/S0100-204X2006001000008
Abstract: studies on the impact of eucalyptus spp. on brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. few studies have focused on microbial diversity and ecology in brazil due to limited coverage of traditional cultivation and isolation methods. molecular microbial ecology methods based on pcr amplified 16s rdna have enriched the knowledge of soils microbial biodiversity. the objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (nfa) and an eucalyptus arboretum (eaa). pcr primers, whose target soil metagenomic 16s rdna were used to amplify soil dna, were cloned using pgem-t and sequenced to determine bacterial diversity. from the nfa soil 134 clones were analyzed, while 116 clones were analyzed from the eaa soil samples. the sequences were compared with those online at the genbank. phylogenetic analyses revealed differences between the soil types and high diversity in both communities. soil from the eucalyptus spp. arboretum was found to have a greater bacterial diversity than the soil investigated from the native forest area.
La descentralización financiera en Chile en la década de los noventa
Oyarzo M. César
Revista Panamericana de Salud Pública , 2000,
A nonautonomous epidemic model with general incidence rate and quarantine
sar M. Silva
Quantitative Biology , 2012,
Abstract: We obtain conditions for eradication and permanence of the infectives for a nonautonomous SIQR model with time-dependent parameters, that are not assumed to be periodic. The incidence is given by functions of all compartments and the threshold conditions are given by some numbers that play the role of the basic reproduction number. We obtain simple threshold conditions in the autonomous, asymptotically autonomous and periodic settings and show that our thresholds coincide with the ones already established. Additionally, we obtain threshold conditions for the general nonautonomous model with mass-action, standard and quarantine-adjusted incidence.
Small mammals of Chapada dos Veadeiros National Park (Cerrado of Central Brazil): ecologic, karyologic, and taxonomic considerations
Bonvicino, C. R.;Lemos, B.;Weksler, M.;
Brazilian Journal of Biology , 2005, DOI: 10.1590/S1519-69842005000300004
Abstract: this work is based on a survey of small mammals carried out in the chapada dos veadeiros national park, a natural reserve located in the mountains of the planalto central goiano in the cerrado of central brazil. the 227 specimens collected represented six marsupial and 13 rodent species. taxonomic, karyologic, and ecologic considerations are present and discussed in the present work. our data reflected the faunal heterogeneity with respect to both elevation and vegetation because only eight of the 19 species were collected at both high and low elevations. the composition of the small mammal fauna of the park is influenced by predominance of forest formations at low elevations and cerrado with rupestrian areas at high elevations. presence of endemic species and one undescribed demonstrated that the cerrado has an endemic fauna and a little known diversity of small mammals.
Managing Waters of the Paraíba do Sul River Basin, Brazil: a Case Study in Institutional Change and Social Learning
Lori M. Kumler,Maria Carmen. Lemos
Ecology and Society , 2008,
Abstract: This article examines the implementation of integrated water-management institutions in the Paraíba do Sul River basin in southeast Brazil. It argues that social learning has been critical in facilitating reform implementation so far, and will likely continue to be an important factor for the future sustainability of the new management system. There has been a synergistic relationship between social learning and Brazil’s water-reform hybrid governance institutions, in which social learning facilitated the implementation of the reform’s new institutions, which in turn enabled further learning in the context of the river basin committee's decision-making process. Through interviews, surveys, and observations, we identified social-learning capacities, including trust, an ability to work together, and the committee’s shared understanding of the institution’s problems, possibilities, and mission. Effective management through social learning was demonstrated by the institution’s adaptive capacity in the face of a severe drought.
Non-Singular Constant Curvature Two-Dimensional Black Hole
José P. S. Lemos,Paulo M. Sá
Physics , 1993, DOI: 10.1142/S0217732394000587
Abstract: We show that the two-dimensional theory of Teitelboim and Jackiw has a black hole solution, with two surprising properties: first, it has constant curvature, and second, is free of spacetime singularities. The maximally extended spacetime consists of an infinite chain of universes connected by timelike wormholes.
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