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Search Results: 1 - 10 of 409638 matches for " Mário;Furtado "
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Efecto Inhibidor del Extracto Acuoso de las Teliosporas Telióides del Hongo Puccinia pampeana Speg., sobre Agentes Causales de Royas
Passador, Martha Maria;Barreto Figueiredo, Mário;Furtado, Edson Luiz;
Revista mexicana de fitopatología , 2008,
Abstract: green pepper rust is caused by the fungus puccinia pampeana. since this fungus has two distinct telial forms, it is classified as a biteliomorphic rust. the first form occurs at the beginning of symptom appearance, with aecidioid, golden-yellow, catenulate teliospores surrounded by a peridium. the second one occurs at the end of the fungus cycle in the host, with dark brown, pulvinate, bicellular, pedicellate telioid teliospores, which contain germination self-inhibiting substances in their walls, that can be removed by washing. aqueous extracts were prepared with 20 mg of puccinia pampeana telioid teliospores, and tested on germination inhibition of aecidioid teliospores of the same rust and of urediniospores of coleosporium plumierae and hemileia vastratrix. the inhibiting effect was demonstrated by measuring metabasidial length (aecidioid teliospores of p. pampeana) and germ tube length of c. plumierae and h. vastratrix. the inhibiting effect was verified after 4 and 6 h of contact of spores with the extracts.
Caracteriza??o morfofisiológica de isolados do gênero Cylindrocladium
Aparecido, Christiane Ceriani;Furtado, Edson Luiz;Figueiredo, Mário Barreto;
Summa Phytopathologica , 2008, DOI: 10.1590/S0100-54052008000100008
Abstract: in order to characterize the anamorphic genus cylindrocladium eighteen isolates of this funguswere cultivated in different culture media, kept under different temperatures, besides having conidia and terminal vesicles morphologically analyzed for correct identification of the isolates. inoculations on different hosts were carried out to evaluate of pathogenic behavior of the studied isolates. concerning the morphologic characters studied, it was observed that alterations occurred in the conidial dimensions and morphology of the terminal vesicles, but was not important considering that is due to substratum changes. however, the natural variability in the general characteristics of these structures indicated that it is difficult to obtain a correct identification of the isolates. in the same culture it was observed terminal vesicles with different morphologies. pathogenic and physiological differences among the isolates could be observed. this brings the idea of distinct population groups when such characteristics are considered in this study. these differences probably indicate a distinct genetic constitution among the isolates used in this study.
Especificidade de Puccinia pampeana a cultivares de Capsicum spp. e outras solanáceas
Passador, Martha Maria;Furtado, Edson Luiz;Figueiredo, Mário Barreto;
Summa Phytopathologica , 2009, DOI: 10.1590/S0100-54052009000100012
Abstract: the capsicum spp. (pepper and green pepper) rust is caused by the fungus puccinia pampeana. this is an important disease in these crops which may cause complete losses in crops of several species of capsicum. although specific to the genus capsicum, and despite the fact that many species within this genus are susceptible to rust, some of them have shown a hypersensitivity reaction. that was the case with capsicum annuum (cayenne pepper) and c. chinense (datil pepper), which, after spermogonia formation (11 days), showed necrotic spots in the peripheral region of the spermogonia 15 days after inoculation, without evolution of the infection. a milder hypersensitivity reaction was also observed only in leaves of c. annuum (chili pepper) and c. baccatum ("chapéu-de-frade"). with regard to other solanaceous plants inoculated ("jiló" and eggplant), no symptoms or signs of infection were observed.
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
Cargnelutti, Juliana Felipetto;Brum, Mário Celso Sperotto;Weiblen, Rudi;Flores, Eduardo Furtado;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000300040
Abstract: west nile virus (wnv) envelope glycoproteins prem/e were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for wnv antibodies. sera from horses, mice and chicken immunized with an inactivated wnv vaccine and, less consistently, sera from horses acutely infected with wnv, reacted specifically with viral antigens present in prem/e-expressing cells.
Selection and characterization of brivudin resistant bovine herpesvirus type 5
Brum, Mário Celso Sperotto;Santos, Cyndia Mara Bezerra dos;Weiblen, Rudi;Flores, Eduardo Furtado;
Brazilian Journal of Microbiology , 2010, DOI: 10.1590/S1517-83822010000100019
Abstract: bovine herpesvirus type 5 (bohv-5) is the agent of meningoencephalitis, an important disease of cattle in south america. the neuropathogenesis of bohv-5 infection is poorly understood and most previous research focused on the role of envelope glicoproteins in neurovirulence. thymidine kinase (tk) is a viral enzyme necessary for virus replication in neurons and, therefore, represents a potential target for virus attenuation. the selection and characterization of bohv-5 variants resistant to the nucleoside analog brivudin (bvdu), which selects tk-defective viruses is here described. several bvdu-resistant clones were obtained after multiple passages in tissue culture in the presence of bvdu and one clone (bohv-5/r-27) was further characterized. the selected clone replicated to similar titers and produced plaques with similar size and morphology to those of wild-type virus (sv507/99). the genetic stability of the resistant virus was demonstrated after ten passages in cell culture in the absence of the drug. moreover, the drug-resistant virus showed reduced virulence in a rabbit model: virus inoculation in four rabbits did not result in disease, in contrast with 75% morbidity (3/4) and 50% mortality (2/2) among rabbits inoculated with the parental virus. these results demonstrate that bohv-5 is sensitive to bvdu and that drug-resistant mutants can be readily selected upon bvdu treatment. bvdu-resistant mutants, likely defective in tk, retained their ability to replicate in tissue culture yet were attenuated for rabbits. this strategy to obtain tk-defective bohv-5 may be useful to study the role of tk in bohv-5 neuropathogenesis and for vaccine development.
Teste imunoenzimático com base em anticorpo monoclonal para a detec??o de anticorpos contra os herpesvírus bovino tipos 1 e 5
Bauermann, Fernando Vi?osa;Brum, Mário Celso Sperotto;Weiblen, Rudi;Flores, Eduardo Furtado;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000500007
Abstract: bovine herpesviruses 1 (bohv-1) and 5 (bohv-5) are antigenic and genetically related viruses associated with different clinical syndromes in cattle, including respiratory, reproductive, neurological disease and abortion. epidemiological studies indicate the widespread distribution of both viruses among brazilian cattle. serological diagnosis, that allows the identification of latently infected animals, represents an important tool for individual and herd monitoring. the present article describes the standardization of a monoclonal antibody (mab)-based immunoenzymatic test (elisa) for detection of antibodies to bohv-1 and/or bohv-5. the initial steps involved the determination of the most suitable mab, the appropriate dilutions of viral antigen and serum samples, and the cut-off value of the assay. after standardization, the elisa was validated by testing 506 cattle serum samples previously tested for neutralizing antibodies to bohv-1 and bohv-5 by virus neutralizing assay (vn). comparing to the vn for bohv-1 antibodies, the elisa presented sensitivity and specificity of 96.6% and 98.3%, respectively. positive and negative predictive values were 97.6%, the concordance between the tests was 97.6% and the coefficient of correlation k (kappa) was 0.95, demonstrating an excellent correlation. comparing to the vn for bohv-5 antibodies, the elisa presented 94.3% of sensitivity, 97.9% of specificity, 97.1% of positive predictive value, 95.9% negative predictive value, concordance of 96.4% and kappa coefficient of 0.92. these results demonstrate that the elisa presents suitable specificity and sensitivity to be used for individual and herd serological diagnosis of bohv-1 and bohv-5, thus, representing an alternative for vn assays and imported elisa kits.
Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E
Brum, Mário Celso S.;Caron, Luizinho;Chowdhury, Shafiqul I.;Weiblen, Rudi;Flores, Eduardo Furtado;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000100009
Abstract: the immunogenicity of an inactivated, experimental vaccine based on a bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein e (bohv-5 ge/tkδ) was evaluated in cattle and the results were compared with a vaccine containing the parental bohv-5 strain (sv507/99). to formulate the vaccines, each virus (wildtype sv507/99 and bohv-5 ge/tk?) was multiplied in cell culture and inactivated with binary ethyleneimine (bei). each vaccine dose contained approximately of 107.5 tcid50 of inactivated virus mixed with an oil-based adjuvant (46:54). forty calves, 6 to 9-months-old, were allocated into two groups of 20 animals each and vaccinated twice (days 0 and 22pv) by the subcutaneous route with either vaccine. serum samples collected at day 0 and at different intervals after vaccination were tested for virus neutralizing (vn) antibodies against the parental virus and against heterologous bohv-5 and bohv-1 isolates. the vn assays demonstrated seroconversion to the respective homologous viruses in all vaccinated animals after the second vaccine dose (mean titers of 17.5 for the wildtype vaccine; 24.1 for the recombinant virus). all animals remained reagents up to day 116 pv, yet showing a gradual reduction in vn titers. animals from both vaccine groups reacted in similar vn titers to different bohv-1 and bohv-5 isolates, yet the magnitude of serological response of both groups was higher against bohv-5 field isolates. calves vaccinated with the recombinant virus did not develop antibodies to ge as verified by negative results in a ge-specific elisa, what would allow serological differentiation from naturally infected animals. taken together, these results indicate that inactivated antigens of bohv-5 ge/tk recombinant virus induced an adequate serological response against bohv-5 and bohv-1 and thus can be used as an alternative, differential vaccine candidate.
Strained In1-xGaxAsyP1-y/InP quantum well heterostructures grown by low-pressure metalorganic vapor phase epitaxy
Carvalho Jr Wilson de,Bernussi Ayrton André,Furtado Mário Tosi,Gobbi ?ngelo Luiz
Materials Research , 1999,
Abstract: We have investigated the optical and the structural properties of strained In1-xGaxAsyP1-y/InP and strain compensated In1-xGaxAsyP1-y/In1-zGazAsqP1-q/InP multi-quantum well heterostructures grown by low-pressure metalorganic vapor phase epitaxy at different growth conditions. Our results indicate an increase of the compositional fluctuation of quaternary materials as the alloy composition moves from the outer spinodal isotherm into the miscibility gap region. In1-xGaxAsyP1-y layers grown at high tensile strained values exhibit a three-dimensional-like growth mode. Strain compensated structures revealed the presence of a broad photoluminescence emission band below the fundamental quantum well transition, well defined elongated features along the [011] direction and interface undulations. All these effects were found to be strongly dependent on the growth temperature and the number of wells.
“Datamining” dos genes da celulose sintase relacionados com ESTs de Eucalyptus spp. (Nota Científica). Cellulose synthase genes dataming related with Eucalyptus spp. expressed sequence tags. (SCIENTIFIC NOTE)
Léo ZIMBACK,Edson Seizo MORI,Mário Luiz Teixeira de MORAES,Edson Luiz FURTADO
Revista do Instituto Florestal , 2008,
Abstract: Trata-se de um estudo sobre “datamining”envolvendo genes ligados ao crescimento decontrole n o hormonal, utilizando o banco dedados de ESTs de eucalipto, efetuado atravésdo Projeto Genoma do Eucalipto (FORESTs)comparados ao nível de aminoácidos. Foramidentificados os clusters de ESTsEGBGFB1211D01.g, EGEZRT6201E10.g,EGCCFB1220G07.g, EGRFCL1206E01.g,EGEQST2006A06.g, EGRFCL1206E01.g,EGEQRT3001H05.b e EGBFRT3106G11.g,similares às proteínas de celulose sintase e suassubunidades controlando o crescimento emArabidopsis thaliana, Gossipium hirsutum,Populus tremuloides, Zea mays e Nicotiana alata,registradas no National Center of BiotechnologiesInformation - NCBI, informa o valiosa parafuturos programas de melhoramento genético dogênero Eucalyptus.This is a study about data mining ofexpressed sequence tags (ESTs) involved withcellulose synthase growth effect genes resultedfrom the Eucalyptus ESTs Genome Project(FORESTs) compared at aminoacids level. Using asequencing of derived from cDNAs librariesinduced and not induced by bacteria, wereidentified EST clusters EGBGFB1211D01.g,EGEZRT6201E10.g, EGCCFB1220G07.g,EGRFCL1206E01.g, EGEQST2006A06.g,EGRFCL1206E01.g, EGEQRT3001H05.b, andEGBFRT3106G11.g, similar to cellulose synthaseproteins controlling growth effect in Arabidopsisthaliana, Gossipium hirsutum, Populustremuloides, Zea mays, and Nicotiana alata,registered on National Center of BiotechnologiesInformation - NCBI. These mining results areimportant to improve Eucalyptus breeding programs.
Enfermidade gastroentérica e respiratória em bezerros inoculados com amostras brasileiras do vírus da diarréia viral bovina tipo 2 (BVDV-2)
Brum Mário Celso Sperotto,Scherer Charles Fernando Capinos,Flores Eduardo Furtado,Weiblen Rudi
Ciência Rural , 2002,
Abstract: Duas amostras brasileiras do vírus da Diarréia Viral Bovina tipo 2 (BVDV-2) foram inoculadas em bezerros com o objetivo de avaliar a sua virulência e estudar a patogenia da infec o. Previamente à inocula o, os animais foram imunodeprimidos com dexametasona. Quatro bezerros com idades entre 45 e 90 dias (grupo A) foram inoculados com a amostra SV-260 (n=2) ou LV-96 (n=2) e quatro bezerros com 6 a 8 meses de idade foram inoculados com a amostra SV-260 (grupo B). Após a inocula o, os bezerros do grupo A apresentaram anorexia, depress o, hipertermia, sinais de infec o respiratória e diarréia profusa, acompanhada de melena em dois animais. Os sinais respiratórios e digestivos progrediram, e os animais morreram ou foram sacrificados in extremis entre os dias 7 e 12 pós-inocula o. úlceras e eros es no trato digestivo (língua, n=4; es fago, n=1; rúmen, n=1 e abomaso, n=3), edema pulmonar (n=4) e na mucosa do abomaso (n=3); equimoses e sufus es na serosa do ba o (n=2), no rúmen, no intestino delgado e no ceco (n=1), no cora o (n=1) e na mucosa da bexiga (n=1) e intussuscep o intestinal (n=1) foram os achados macroscópicos mais marcantes. úlceras e eros es, acompanhadas de infiltrado mononuclear na mucosa e submucosa do trato digestivo e deple o linfóide nos linfonodos e placas de Peyer, foram as altera es microscópicas mais freqüentes. O vírus foi detectado em vários tecidos e órg os. Antígenos virais foram demonstrados por imuno-histoquímica, principalmente em células epiteliais do trato digestivo; em células mononucleares nos espa os perivasculares e peribronquiais; na cápsula e septos de linfonodos; e em linfócitos e células mononucleares das placas de Peyer e ba o. Os animais do grupo B apresentaram depress o, hipertermia, sinais moderados de infec o respiratória e digestiva, ulcera es na língua e bochecha, mas recuperaram-se após alguns dias. Esses resultados demonstram que as amostras de BVDV-2 foram capazes de reproduzir a enfermidade aguda quando inoculadas em bezerros e que as conseqüências clínico-patológicas da infec o foram mais severas nos animais mais jovens.
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