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Search Results: 1 - 10 of 550817 matches for " Márcia de Carvalho;Lacerda-Queiroz "
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Behavioral investigation of mice with experimental autoimmune encephalomyelitis
Rodrigues, David Henrique;Vilela, Márcia de Carvalho;Lacerda-Queiroz, Norinne;Miranda, Aline Silva de;Sousa, Larissa Fonseca da Cunha;Reis, Helton José dos;Teixeira, Ant?nio Lúcio;
Arquivos de Neuro-Psiquiatria , 2011, DOI: 10.1590/S0004-282X2011000700018
Abstract: multiple sclerosis is a neuroinflammatory disease that results in serious neurological disability. besides physical impairment, behavioral symptoms are also common in patients with multiple sclerosis. experimental autoimmune encephalomyelitis (eae) is considered to be a model of multiple sclerosis and mimics the main features of the disease, such as demyelination and motor impairment. in this work, we aimed to study behavioral parameters in animals with eae using the mog35-55 model in c57bl/6 mice. we analyzed memory and anxiety in animals using the elevated plus maze, the step down inhibitory avoidance task and the memory recognition test. no differences in any tests were found when comparing controls and animals induced with eae. therefore, we conclude that behavioral changes in animals with eae induced with mog35-55 are probably subtle or absent.
Increased levels of glutamate in the central nervous system are associated with behavioral symptoms in experimental malaria
Miranda, A.S.;Vieira, L.B.;Lacerda-Queiroz, N.;Souza, A.H.;Rodrigues, D.H.;Vilela, M.C.;Gomez, M.V.;Machado, F.S.;Rachid, M.A.;Teixeira, A.L.;
Brazilian Journal of Medical and Biological Research , 2010, DOI: 10.1590/S0100-879X2010007500130
Abstract: cerebral malaria (cm) is a severe complication resulting from plasmodium falciparum infection. this condition has been associated with cognitive, behavioral and motor dysfunctions, seizures and coma. the underlying mechanisms of cm are incompletely understood. glutamate and other metabolites such as lactate have been implicated in its pathogenesis. in the present study, we investigated the involvement of glutamate in the behavioral symptoms of cm. seventeen female c57bl/6 mice (20-25 g) aged 6-8 weeks were infected with p. berghei anka by the intraperitoneal route using a standardized inoculation of 106 parasitized red blood cells suspended in 0.2 ml pbs. control animals (n = 17) received the same volume of pbs. behavioral and neurological symptoms were analyzed by the smithkline/harwell/imperial college/royal hospital/phenotype assessment (shirpa) battery. glutamate release was measured in the cerebral cortex and cerebrospinal fluid of infected and control mice by fluorimetric assay. all functional categories of the shirpa battery were significantly altered in the infected mice at 6 days post-infection (dpi) (p ≤ 0.05). in parallel to cm symptoms, we found a significant increase in glutamate levels in the cerebral cortex (mean ± sem; control: 11.62 ± 0.90 nmol/mg protein; infected at 3 dpi: 10.36 ± 1.17 nmol/mg protein; infected at 6 dpi: 26.65 ± 0.73 nmol/mg protein; with egta, control: 5.60 ± 1.92 nmol/mg protein; infected at 3 dpi: 6.24 ± 1.87 nmol/mg protein; infected at 6 dpi: 14.14 ± 0.84 nmol/mg protein) and in the cerebrospinal fluid (control: 128 ± 51.23 pmol/mg protein; infected: 301.4 ± 22.52 pmol/mg protein) of infected mice (p ≤ 0.05). these findings suggest a role of glutamate in the central nervous system dysfunction found in cm.
Intracerebral infection with dengue-3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice
Debora CG Amaral, Milene A Rachid, Marcia C Vilela, Roberta DL Campos, Gustavo P Ferreira, David H Rodrigues, Norinne Lacerda-Queiroz, Aline S Miranda, Vivian V Costa, Marco A Campos, Erna G Kroon, Mauro M Teixeira, Antonio L Teixeira
Journal of Neuroinflammation , 2011, DOI: 10.1186/1742-2094-8-23
Abstract: C57BL/6 mice received 4 × 103 PFU of DENV-3 by an intracranial route. We evaluated the trafficking of leukocytes in brain microvasculature using intravital microscopy, and evaluated chemokine and cytokine profiling by an ELISA test at 3 and 6 days post infection (p.i.). Furthermore, we determined myeloperoxidase activity and immune cell populations, and also performed histopathological analysis and immunostaining for the virus in brain tissue.All animals developed signs of encephalitis and died by day 8 p.i. Motor behavior and muscle tone and strength parameters declined at day 7 p.i. We observed increased leukocyte rolling and adhesion in brain microvasculature of infected mice at days 3 and 6 p.i. The infection was followed by significant increases in IFN-γ, TNF-α, CCL2, CCL5, CXCL1, and CXCL2. Histological analysis showed evidence of meningoencephalitis and reactive gliosis. Increased numbers of neutrophils, CD4+ and CD8+ T cells were detected in brain of infected animals, notably at day 6 p.i. Cells immunoreactive for anti-NS-3 were visualized throughout the brain.Intracerebral infection with non-adapted DENV-3 induces encephalitis and behavioral changes that precede lethality in mice.Dengue, one of the most important arboviral human diseases, is a serious cause of morbidity and mortality in tropical and subtropical regions of the world. Approximately 2.5 billion people are at risk of being infected by dengue virus [1]. The dengue virus (DENV) comprises four serotypes - DENV-1, DENV-2, DENV-3, and DENV-4, all of which appear to be present in 22 of the 27 states of Brazil [2-4].Although DENV has been considered a non-neurotropic virus in humans, some authors have described the presence of the virus in cerebrospinal fluid (CSF), and dengue antigens in brain tissue [5,6]. Moreover, infection has been associated with encephalitis, acute disseminated encephalomyelitis, neuropathies, and Guillain-Barré syndrome [6-8]. Therefore, dengue infection should be considered a
Role of IL-4 in an experimental model of encephalitis induced by intracranial inoculation of herpes simplex virus-1 (HSV-1)
Vilela, Márcia Carvalho;Campos, Roberta Dayrell de Lima;Mansur, Daniel Santos;Rodrigues, David Henrique;Queiroz, Norinne Lacerda;Lima, Graciela Kunrath;Rachid, Milene Alvarenga;Kroon, Erna Geessien;Campos, Marco Ant?nio;Teixeira, Ant?nio Lúcio;
Arquivos de Neuro-Psiquiatria , 2011, DOI: 10.1590/S0004-282X2011000200019
Abstract: herpes simplex virus-1 (hsv-1) is a pathogen that may cause severe encephalitis in humans. in this study, we aimed to investigate the role of interleukin-4 (il-4) in a model of hsv-1 brain infection. il-4 knockout (il-4-/-) and wild type (wt) c57bl/6 mice were inoculated with 104 plaque-forming units of hsv-1 by the intracranial route. histopathologic analysis revealed a distinct profile of infiltrating cells at 3 days post-infection (dpi). infected wt mice presented mononuclear inflammatory cells while il-4-/- mice developed meningoencephalitis with predominance of neutrophils. il-4-/- mice had diminished leukocyte adhesion at 3 dpi when compared to infected wt animals in intravital microscopy study. conversely no differences were found in cerebral levels of cxcl1, cxcl9, ccl3, ccl5 and tnf-α between wt and il-4-/- infected mice. il-4 may play a role in the recruitment of cells into central nervous system in this acute model of severe encephalitis caused by hsv-1.
As representa??es da malária na obra de Jo?o Guimar?es Rosa
Lacerda-Queiroz, Norinne;Queiroz Sobrinho, Ant?nio;Teixeira, Ant?nio Lúcio;
História, Ciências, Saúde-Manguinhos , 2012, DOI: 10.1590/S0104-59702012000200007
Abstract: the article discusses the historical, scientific and literary aspects of malaria, with an emphasis on images of the disease in the work of the writer jo?o guimar?es rosa. the main reference for this study is the short story "sarapalha," which is featured in the book entitled sagarana. the author's medical training together with his experiences in the hinterlands of the country is the subject matter for the work, with stories of the harsh reality of life in the outback. a highlight of the story is the narrative of malaria in the language of the outback, though with absolute medical and scientific precision.
Rendimento e composi??o químico-bromatológica de fenos triturados de gramíneas tropicais
Aguiar, Emerson Moreira de;Lima, Guilherme Ferreira da Costa;Santos, Mércia Virgínia Ferreira dos;Carvalho, Francisco Fernando Ramos de;Guim, Adriana;Medeiros, Henrique Rocha de;Borges, Aurinês Queiroz;
Revista Brasileira de Zootecnia , 2006, DOI: 10.1590/S1516-35982006000800005
Abstract: the research was carried out to evaluate yield, chemical composition and losses of chopped tropical grass hays with the following forages: pearl millet (pennisetum americanum), sudangrass (sorghum sudanense), elephantgrass (pennisetum purpureum) and two cultivars of forage sorghum (sf-25 and ipa-467-4-2) (sorghum bicolor). the materials were harvested at 30% flowering, except for elephantgrass (60 days). the experiment was conducted according to a complete randomized block design, with five treatments and four replications. there were significant differences among hay productions with sorghum cultivars ipa-467-4-2 and sf-25 reaching the highest yields (10.85 and 10.65 t/ha/cut). production of elephantgrass, sudangrass and pearl millet hays were respectively of 6.94, 6.69, and 4.93 t/ha/cut. percentage of hay losses were not different among all treatments and varied from 17.33 to 20.17%. pearl millet and sudangrass hays had the highest cp concentrations (10.56 and 8.80%), which were superior to elephantgrass (6.76%) and sorghum cultivars (5.62 and 5.50%). most of the ndf values were superior to 70.0%. sorghum cultivars exhibited the lowest ndin concentration (0.42 and 0.40% of dm). the adin concentration ranged from 0.06 to 0.30% of dm. the highest lignin (%dm) concentration was observed for pearl millet (6.52%) and sorghum ipa-467-4-2 (6.17%) hays. estimated tdn of the hays showed significant differences and sudangrass reached the highest value (53.35%). production of chopped tropical grass hays showed high yields and chemical composition according to the minimum standards for ruminant nutrition.
A vida por um trabalho: popula o estrangeira e ilegal na Espanha
Márcia Siqueira de Carvalho
Confins , 2008, DOI: 10.4000/confins.1792
Abstract: Os dados do recenseamento revelaram um perfil da popula o estrangeira na Espanha, confirmaram algumas impress es e desfizeram outras. Eles refletem também a desigualdade na distribui o dos aumentos demográficos no território daquele país, com o destaque para a costa mediterranea, a regi o da Catalunha (Nordeste do país) e na cidade de Madri e arredores, enquanto houve perda populacional no interior e na regi o norte. O peso da popula o estrangeira na popula o total da Espanha cresceu de 7,0% em 2004 para 8,5% em 2005. Apesar da dificuldade na análise dos dados populacionais em rela o aos estrangeiros legalizados e os ilegais, essa tendência n o será revertida t o cedo. Setores da economia espanhola dependem da imigra o, legal ou n o. A Caixa Catalunya, entidade financeira desta comunidade aut noma, estimou que 50% do crescimento da PIB catal o nos últimos anos deve-se ao fen meno da imigra o. Les données du recensement ont révélé un profil de la population étrangère en Espagne, ont confirmé quelques impressions et en ont défait autres. Ils reflètent aussi l'inégalité dans la distribution des augmentations démographiques dans le territoire de ce pays, avec la prééminence de la c te méditerranéenne, de la Catalogne (Nord-est du pays) et de la ville de Madrid et de sa banlieue, alors que l'intérieur et la région nord ont vu leur population décliner. Le poids de la population étrangère dans la population totale de l'Espagne a grandi de 7,0% dans 2004 à 8,5% en 2005. Malgré la difficulté de l'analyse des données démographiques concernant les étrangers légalisés et les illégaux, cette tendance ne se retournera pas de sit t. Des secteurs de l'économie espagnole dépendent de l'immigration, légale ou non. La Caixa Catalunya, organisme financier de cette communauté indépendante, a estimé que 50% de la croissance du PIB catalan dans les dernières années est due au phénomène de l'immigration. The data of the census had disclosed a profile of the foreign population in Spain, had confirmed some impressions and had undone others. They also reflect the inequality in the distribution of the demographic increases in the territory of that country, with the prominence for the Mediterranean coast, the region of Catalonia (Northeast of the country) and in the city of Madrid and the outskirts, while she had population loss in the interior and the region north. The weight of the foreign population in the total population of Spain grew of 7,0% in 2004 for 8,5% in 2005. Despite the difficulty in the analysis of the population data in relation to the legalized foreigners a
A DNA Vaccine against Yellow Fever Virus: Development and Evaluation
Milton Maciel Jr.?,Fábia da Silva Pereira Cruz?,Marli Tenório Cordeiro?,Márcia Archer da Motta?,Klécia Marília Soares de Melo Cassemiro?,Rita de Cássia Carvalho Maia?,Regina Célia Bressan Queiroz de Figueiredo?,Ricardo Galler?,Marcos da Silva Freire?,Joseph Thomas August
PLOS Neglected Tropical Diseases , 2015, DOI: 10.1371/journal.pntd.0003693
Abstract: Attenuated yellow fever (YF) virus 17D/17DD vaccines are the only available protection from YF infection, which remains a significant source of morbidity and mortality in the tropical areas of the world. The attenuated YF virus vaccine, which is used worldwide, generates both long-lasting neutralizing antibodies and strong T-cell responses. However, on rare occasions, this vaccine has toxic side effects that can be fatal. This study presents the design of two non-viral DNA-based antigen formulations and the characterization of their expression and immunological properties. The two antigen formulations consist of DNA encoding the full-length envelope protein (p/YFE) or the full-length envelope protein fused to the lysosomal-associated membrane protein signal, LAMP-1 (pL/YFE), aimed at diverting antigen processing/presentation through the major histocompatibility complex II precursor compartments. The immune responses triggered by these formulations were evaluated in H2b and H2d backgrounds, corresponding to the C57Bl/6 and BALB/c mice strains, respectively. Both DNA constructs were able to induce very strong T-cell responses of similar magnitude against almost all epitopes that are also generated by the YF 17DD vaccine. The pL/YFE formulation performed best overall. In addition to the T-cell response, it was also able to stimulate high titers of anti-YF neutralizing antibodies comparable to the levels elicited by the 17DD vaccine. More importantly, the pL/YFE vaccine conferred 100% protection against the YF virus in intracerebrally challenged mice. These results indicate that pL/YFE DNA is an excellent vaccine candidate and should be considered for further developmental studies.
Description of socioeconomic and demographic profile of young women vulnerable to infection by human papillomavirus and risk behavior in a school in Rio de Janeiro  [PDF]
M.C. de Melo Pessanha Carvalho, Ana Beatriz Azevedo Queiroz, Maria Aparecida Vasconcelos Moura
Health (Health) , 2013, DOI: 10.4236/health.2013.511246
Abstract: Background: HPV is one of the main sexually transmitted diseases, especially among the female population. This is an important etiologic agent for the development of cervical intraepithelial lesions and cervical cancer. It is considered a public health problem, since young women are the most vulnerable group to this virus. Therefore, it is important that the socioeconomic and demographic profile of these women and their risk behaviors are known, so that it is possible to contribute in reducing infection occurrences in the studied population. Objectives: To describe the socioeconomic and demographic characteristics and investigate the behavioral sexual-affective aspects of risk of adolescents and young students from Rio de Janeiro/Brazil when tackling HPV infection. Methods: A group composed by 128 individuals susceptible to HPV—classified as adolescent women and young women who are students at a high school in one unity of the municipality of Rio de Janeiro. The studied period was from May to November. A quantitative descriptive approach was used, in which data were highlighted in variables, divided into economic, demographic and behavioral characteristics. Data were entered into an Excel spreadsheet and organized by descriptive statistics performed by simple frequency (%). Results: The age range of the young women who have the possibility of being infected with HPV was from 15 to 25. The focused family income among these young women was up to 2 minimum wages. The risk behavior detected in 37.5% of adolescent women and in 43.8% of young women is that these women never used condoms in sexual intercourse. Conclusion: The research showed that the studied women are vulnerable due to risk behavior practices that may lead to the virus acquisition. More focuses on educational actions of preventive measures regarding HPV infection should be emphasized, favoring a lower incidence of human papillomavirus infection and cervical cancer.
Ensino universitário, corpora??o e profiss?o: paradoxos e dilemas brasileiros
Nunes, Edson;Carvalho, Márcia Marques de;
Sociologias , 2007, DOI: 10.1590/S1517-45222007000100008
Abstract: this article examines the relationship between higher education and professional occupation, based on data from the 2000 demographic census (ibge), stressing occupations related to regulated professions. a large gap is seen between having a formal diploma and the real occupation of graduates. it considers that noncorrespondence between education and occupation is due mainly to the educational model, historically tied to regulated professions, which no longer meets the reality of brazilian society.
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