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Search Results: 1 - 10 of 38 matches for " Luteolin "
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Template-Monomer Interaction in Molecular Imprinting: Is the Strongest the Best?  [PDF]
Xuewen Fu, Qiang Yang, Qiang Zhou, Qiang Lin, Chun Wang
Open Journal of Organic Polymer Materials (OJOPM) , 2015, DOI: 10.4236/ojopm.2015.52007
Abstract: Molecularly imprinted polymers (MIPs) represent a new class of materials possessing high selectivity and affinity for the target molecule. They have been utilized as sensors, catalysts, sorbents for solid-phase extraction, stationary phase for liquid chromatography, mimics of enzymes, receptors, and antibodies. In this research, molecular imprinted polymers (MIPs) for luteolin were prepared using acrylamide, 4-vinylpyridine and 1-allyl-piperazine as functional monomers and ethylene glycol dimethacrylate as cross-linker by non-covalent imprinting method. UV-visible spectra were used to evaluate the interaction strength between the template and the monomers. The composites of the polymers were calculated from elementary analysis. The porous properties of the imprinted polymers have been determined from nitrogen adsorption-desorption isotherms. The imprinting efficiency of the prepared MIPs was evaluated by selective adsorption for luteolin and its structural analogues. Although the interaction strength of monomers to the template was in the order 1-ALPP > 4-VP > AA, the binding affinity of the imprinted polymers towards luteolin was in the order MIP 2 > MIP 3 > MIP 1. Our results demonstrated that the imprinting efficiency was depending not only on the interaction strength between the template and the monomer, but also on the fidelity in transferring the complex into the polymer.
Additive Protective Effects of Luteolin and Pyruvate against 6-Hydroxydopamine and 3-Hydroxykynurenine Induced Neurotoxicity in SH-SY5Y Cells  [PDF]
Natalia Wszelaki, Matthias F. Melzig
Pharmacology & Pharmacy (PP) , 2013, DOI: 10.4236/pp.2013.44053
Abstract:

Oxidative stress has been implicated as one of the causes in cell death in many neurodegenerative disorders. Due to antioxidative properties in vitro, the use of flavonoids and other polyphenolic compounds synthesised by plants are considered to be a promising strategy to prevent Alzheimer’s disease and Parkinsons’s disease. In the present study, we tested protective effects of some polyphenols and sodium pyruvate on 6-hydroxydopamine (6-OHDA), salsolinol and 3-hydroxykynurenine (3-HK) induced neurotoxicity in human neuroblastoma SH-SY5Y cells. We found that luteolin prevented from 6-OHDA and 3-HK induced cell viability reduction and that one of the mechanisms involved in the neuroprotective process was the ability to increase the level of cellular ATP. However, luteolin was ineffective against salsolinol-induced toxicity. Neither pre-treatment with flavonoids nor simultaneous addition had any protective effects on 6-OHDA, salsolinol or 3-HK induced neurotoxicity. Interestingly, both pre-treatment and co-treatment with pyruvate provided protection against 6-OHDA, salsolinol or 3-HK induced toxicity. Moreover, luteolin and sodium pyruvate, administered together, acted additively, so to achieve the same effect, lower concentrations were needed. The ability of luteolin and sodium pyruvate to reduce toxicity of 6-OHDA and 3-HK in SH-SY5Y cells may be related to two different neuroprotective mechanisms and the capability to penetrate into the cell.

Anti-carcinogenic Effects of the Flavonoid Luteolin
Günter Seelinger,Irmgard Merfort,Ute W?lfle,Christoph M. Schempp
Molecules , 2008, DOI: 10.3390/molecules13102628
Abstract: Luteolin is a flavonoid which is part of our daily nutrition in relatively low amounts (less than 1 mg/day). Nevertheless, some epidemiological studies suggest an inverse correlation between luteolin intake and the risk of some cancer types. Luteolin displays specific anti-inflammatory and anti-carcinogenic effects, which can only partly be explained by its anti-oxidant and free radical scavenging capacities. Luteolin can delay or block the development of cancer cells in vitro and in vivo by protection from carcinogenic stimuli, by inhibition of tumor cell proliferation, by induction of cell cycle arrest and by induction of apoptosis via intrinsic and extrinsic signaling pathways. When compared to other flavonoids, luteolin was usually among the most effective ones, inhibiting tumor cell proliferation with IC50 values between 3 and 50 μM in vitro and in vivo by 5 to 10 mg/kg i.p., intragastric application of 0.1–0.3 mg/kg/d, or as food additive in concentrations of 50 to 200 ppm. Luteolin has been shown to penetrate into human skin, making it also a candidate for the prevention and treatment of skin cancer.
Anti-carcinogenic Effects of the Flavonoid Luteolin
G????nter Seelinger,Irmgard Merfort,Ute W????lfle,Christoph M. Schempp
Molecules , 2008,
Abstract: Luteolin is a flavonoid which is part of our daily nutrition in relatively low amounts (less than 1 mg/day). Nevertheless, some epidemiological studies suggest an inverse correlation between luteolin intake and the risk of some cancer types. Luteolin displays specific anti-inflammatory and anti-carcinogenic effects, which can only partly be explained by its anti-oxidant and free radical scavenging capacities. Luteolin can delay or block the development of cancer cells in vitro and in vivo by protection from carcinogenic stimuli, by inhibition of tumor cell proliferation, by induction of cell cycle arrest and by induction of apoptosis via intrinsic and extrinsic signaling pathways. When compared to other flavonoids, luteolin was usually among the most effective ones, inhibiting tumor cell proliferation with IC50 values between 3 and 50 M in vitro and in vivo by 5 to 10 mg/kg i.p., intragastric application of 0.1 ¢ € “0.3 mg/kg/d, or as food additive in concentrations of 50 to 200 ppm. Luteolin has been shown to penetrate into human skin, making it also a candidate for the prevention and treatment of skin cancer.
Effects of Luteolin on Liver, Kidney and Brain in Pentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities
Hüsniye Birman,Kadriye Akgün Dar,Ay?egül Kapucu,Samet Acar
Balkan Medical Journal , 2012,
Abstract: Objective: Flavonoids are an important group of recognized antioxidants in plants. Luteolin (LUT) is a natural flavonoid in the plant kingdom. This study was aimed to investigate the effects of the LUT in the liver, kidney and brain of pentylentetrazol (PTZ)-induced seizure and the relationship between nitric oxide synthases (iNOS, eNOS) and matrix metalloproteinases (MMP2, MMP9). Materials and Methods: LUT (10 mg/kg) was given intraperitoneally during two weeks prior to seizure induction. A single dose PTZ 80 mg/kg i.p. was administered and seizures were observed and evaluated with regard to latency, frequency and stage for one hour. Results: Seizure frequen cy after PTZ administration was significantly decreased in LUT pretreated rats (p<0.05). An increase of immunhistochemical reactions of iNOS and MMP2, but a decrease of eNOS activity, were observed in rat hippocampus and peripheral tissues during the PTZ induced seizures. LUT pretreatment reversed the iNOS and MMP2 activity to the control levels and significantly increased the eNOS activity (p<0.001). Conclusion: LUT seems to have an effective role in reducing the seizure frequency and a protective role on peripheral organ injury in animal models of seizure. The protective effect of LUT in seizures and the seizure induced peripheral tissue damage warrant further investigations.
Effects of Indole-3-Carbinol and Flavonoids Administered Separately and in Combination on Nitric Oxide Production and iNOS Expression in Rats  [PDF]
Evita Rostoka, Larisa Baumane, Sergejs Isajevs, Aija Line, Karina Silina, Maija Dzintare, Darja Svirina, Jelena Sharipova, Ivars Kalvinsh, Nikolajs Sjakste
Chinese Medicine (CM) , 2010, DOI: 10.4236/cm.2010.11002
Abstract: Beneficial effects of natural compounds are often attributed to modulation of NO production; however effects produced by plant extracts do not correlate with effects of purified components. The goal of our work was to study ability of flavonoids and indole-3-carbinol, as well as their combinations to modify NO production, iNOS gene and protein expression in rat tissues. Baicalein and luteolin decreased NO concentration in both intact and LPS-treated animals. Baicalein decreased iNOS gene expression. Luteolin decreased NO production in the liver and heart and number of iNOS-positive cells in the liver of LPS-treated animals. Combination of the two substances did not decrease the NO synthesis triggered by LPS, although it decreased iNOS gene expression. Quercetin decreased NO production in the heart, kidneys and blood of intact rats, but enhanced the LPS effect in testes, spleen and blood on NO production and iNOS protein expression in the liver. Indole-3-carbinol decreased NO concentration in the cerebellum, blood, lungs and skeletal muscles. The drug enhanced the LPS-triggered increase of NO production in all rat organs. It increased iNOS protein expression in intact liver; however it decreased the LPS-triggered outburst of the enzyme biosynthesis. Combination of indole3-carbinol with quercetin decreased NO production in LPS-treated animals however it slightly increased iNOS gene expression. Taken together our results suggest that modifications of NO level in tissues by a natural compound cannot be predicted from data about its effects on NOS expression or activity. Combination of substances can produce an effect differing from that of individual substances. This stresses importance of direct measurements of NO in the tissues.
Correlation among Antioxidant, Antimicrobial, Hemolytic, and Antiproliferative Properties of Leiothrix spiralis Leaves Extract
Marcelo Gonzaga de Freitas Araújo,Felipe Hilário,Wagner Vilegas,Lourdes Campaner dos Santos,Iguatemy Louren?o Brunetti,Claudia Elena Sotomayor,Tais Maria Bauab
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms13079260
Abstract: The biological activities of a plant extract depend on a complex sum of individual properties including the antioxidant activity. Several biological activities protect against the harmful action of reactive oxygen species (ROS), and here we focused our attention on the relationship between the biological activities tested and the antioxidant properties. In this study, the total flavonoid content as well as the antioxidant, antimicrobial, hemolytic and cytotoxicity activities of the methanolic extract of Leitothrix spiralis leaves were evaluated. The extract showed a total flavonoid content of 19.26% and the chemical characterization by HPLC-PAD confirmed the presence of flavonoids as the major secondary metabolite compounds. Significant antioxidant activity (IC 50 = 1.743 μg/mL ± 0.063) was demonstrated and was effective against Gram-negative organisms and all Candida strains tested, and showed an ability to inhibit hyphal formation. Non-hemolytic and antiproliferative activity could be demonstrated.
Physicochemical Properties and Antioxidant Activities of Luteolin-Phospholipid Complex
Keyong Xu,Benguo Liu,Yuxiang Ma,Jiquan Du,Guanglei Li,Han Gao,Yuan Zhang,Zhengxiang Ning
Molecules , 2009, DOI: 10.3390/molecules14093486
Abstract: luteolin and phospholipid complex was prepared to improve the lipophilic properties of luteolin. The physicochemical properties of the complex were analyzed by ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), X-ray diffractometry (XRD) and differential scanning calorimetry (DSC). The results showed that luteolin and phospholipid in the complex were joined by non-covalent-bonds and did not form a new compound. It was found that the complex was an effective scavenger of DPPH radicals, with an IC50 value of 28.33 μg/mL. In the Rancimat antioxidant test using lard oil as substrate, the complex also showed the strong antioxidant activity.
Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells
Jian Wu, Wenshi Yi, Linhong Jin, Deyu Hu, Baoan Song
Cell Division , 2012, DOI: 10.1186/1747-1028-7-20
Abstract: Ten compounds were extracted and isolated from B. davidii, among which colchicine was identified in B. davidii for the first time. The inhibitory activities of these compounds were investigated in Mgc-803, Bcap-37 cells in vitro by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, and the results showed that luteolin and colchicine had potent inhibitory activities against the growth of Mgc-803 cells. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in Mgc-803 cells. The results also showed that the percentages of early apoptotic cells (Annexin V+/PI-, where PI is propidium iodide) and late apoptotic cells (Annexin V+/PI+) increased in a dose- and time-dependent manner. After 36 h of incubation with luteolin at 20 μM, the percentages of cells were approximately 15.4% in early apoptosis and 43.7% in late apoptosis; after 36 h of incubation with colchicine at 20 μM, the corresponding values were 7.7% and 35.2%, respectively.Colchicine and luteolin from B. davidii have potential applications as adjuvant therapies for treating human carcinoma cells. These compounds could also induce apoptosis in tumor cells.Buddleja belongs to the Loganiaceae family and has a pantropical distribution across South Asia, Africa, and America [1]. This genus comprises approximately 100 species of wood perennials and shrubs. The roots, leaves, and flowers of various species of Buddleja are used in folk medicine in several parts of the world [2]. Various bioactivities, including antimicrobial activity against Staphylococcus aureus, as well as antihepatotoxic, antirheumatic, antiprotozoal, and antifungal properties of isolated compounds from Buddleja have been reported [3-10]. The application of the poultice or lotion of a number of species of Buddleja to treat wounds has also been documented [11,12].Buddleja davidii is a perennial herbaceous plant widely distributed in the Chinese provinces of Yunnan,
Pyrrolidine Dithiocarbamate (PDTC) Attenuates Luteolin-Induced Apoptosis in Human Leukemia HL-60 Cells  [PDF]
Ming-Fen Lee, Cheng-Ta Li, Ming-Dian Chen, An-Chin Cheng
Journal of Cancer Therapy (JCT) , 2012, DOI: 10.4236/jct.2012.36147
Abstract: Studies have indicated that flavonoid luteolin is a potential inhibitor of tumor cell proliferation and may function as an anticarcinogenic agent. Pyrrolidine dithiocarbamate (PDTC), a synthetic compound, may exhibit biphasic effects on apoptosis depending on the experimental context. Previously, we found that luteolin induced the activation of the proapoptotic proteins, such as Bad, Bid, and Bax, in HL-60 human leukemia cells. We also explored the modulatory effects and molecular mechanisms of PDTC on the cytotoxicity of luteolin in HL-60 cells; PDTC could interfere with luteolin’s ability to cleave poly(ADP-ribose)-polymerase (PARP) and DNA fragmentation of factor-45 (DFF-45). In the current study, we further investigated the effect of PDTC on the luteolin-induced death-receptor pathway and the cleavage of the Bcl-2 family members. We found that the combination of luteolin and PDTC increased the survival of the HL-60 cells such that PDTC inhibited both extrinsic and intrinsic pathways in luteolin-induced apoptosis.
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