oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

4 ( 1 )

2019 ( 89 )

2018 ( 208 )

2017 ( 170 )

Custom range...

Search Results: 1 - 10 of 82306 matches for " Luiz Hildebrando Pereira da;Orlandi "
All listed articles are free for downloading (OA Articles)
Page 1 /82306
Display every page Item
Rotavirus and adenovirus in Rond?nia
Magalh?es, Gleiciene Félix;Nogueira, Paulo Afonso;Grava, Andréa Fagundes;Penati, Marilene;Silva, Luiz Hildebrando Pereira da;Orlandi, Patricia Puccinelli;
Memórias do Instituto Oswaldo Cruz , 2007, DOI: 10.1590/S0074-02762007005000067
Abstract: acute gastroenteritis is one of the most common diseases in humans worldwide. viral gastroenteritis is a global problem in infants and young children. in this study the incidence of diarrhea was assessed in 877 hospitalized children under five years old, over a period of 24 months and distributed in 470 cases of diarrhea and 407 age-matched group with other pathologies, as control group. two antigen detection techniques based on enzyme immunoassay (eia) and latex particles were used for detection of rotavirus and adenovirus. rotavirus a was a major cause of gastroenteritis with 23.6% of cases, being 90% of these cases in young children. adenovirus infections was detected by eia with frequency of 6.4%. rotavirus and adenovirus were detected in 10.1 and 1.7% of stools from control group, respectively. interestingly, the frequency of the youngest children in the control group excreting rotavirus a was comparable to that detected in stools from diarrheic children. we cannot rule out the existence of other enteric viruses because the etiology of 171 cases of diarrhea was not determined and active search for astrovirus and calicivirus was not done. this is the first study that shows the presence of enteric viruses in the infantile population from western brazilian amazonia and it was important to help physicians in the treatment of viral gastroenteritis.
Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
Silva, Tatiane;Nogueira, Paulo Afonso;Magalh?es, Gleiciene Félix;Grava, Andréa Fagundes;Silva, Luiz Hildebrando Pereira da;Orlandi, Patrícia Puccinelli;
Memórias do Instituto Oswaldo Cruz , 2008, DOI: 10.1590/S0074-02762008000700017
Abstract: the incidence of shigella spp. was assessed in 877 infants from the public hospital in rond?nia (western amazon region, brazil) where shigella represents the fourth cause of diarrhea. twenty-five isolates were identified: 18 were shigella flexneri, three shigella sonnei, three shigella boydii and one shigella dysenteriae. with the exception of s. dysenteriae, all shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances. pcr detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared among 25 patients testing positive for shigella. the ipah and ipabcd genes were detected in almost all isolates and, unsurprisingly, all shigella isolates associated with colitis were able to invade hela cells. this work alerts for multiple antibiotic resistant shigella in the region and characterizes presence of ipa virulence genes and invasion phenotypesin dysenteric shigellosis.
Enteropathogens associated with diarrheal disease in infants of poor urban areas of Porto Velho, Rond?nia: a preliminary study
Orlandi, Patrícia Puccinelli;Silva, Tatiane;Magalh?es, Gleiciene Felix;Alves, Fabiana;Cunha, Roberto Penna de Almeida;Durlacher, Rui;Silva, Luiz Hildebrando Pereira da;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000500005
Abstract: one hundred and thirty cases of diarrhea and 43 age-matched controls, 0 to 5 years old, were studied in a pediatric outpatient unit from a poor peri urban area of porto velho, rond?nia. eighty percent of diarrheal cases were observed in the groups under 2 years of age. rotavirus (19.2%) was the most frequent enteropathogen associated with diarrhea, followed by shigella flexneri (6.15%) and s. sonnei (1.5%) and salmonella sp. (6.9%). four cases of e. coli enterotoxigenic infections (3.1%), e. coli enteropathogenic (epec)(2.3%) one case of e. coli enteroinvasive infection (0.8%) and one case of yersinia enterocolitica (0.8%) were also identified. mixed infections were frequent, associating rotavirus, epec and salmonella sp. with entamoeba histolytica and giardia lamblia.
Ciências biológicas e biotecnologia: realidades e virtualidades
SILVA, LUIZ HILDEBRANDO PEREIRA DA;
S?o Paulo em Perspectiva , 2000, DOI: 10.1590/S0102-88392000000300011
Abstract: os enormes progressos das ciências biológicas ocorridos nas últimas décadas abrem grandes perspectivas benéficas para o homem no terreno das aplica??es, em particular no domínio da agronomia e da biomedicina. a sociedade brasileira deverá romper com essa situa??o, em que os progressos beneficiam apenas setores privilegiados, e promover a educa??o e divulga??o científicas de qualidade a todos os níveis, concentrando sua aplica??o em domínios essenciais para vencer atrasos e deforma??es da sociedade brasileira. se a ciência e a tecnologia n?o tiverem rela??o direta com a realidade do dia-a-dia, traduzindo-se em melhoria da situa??o material e cultural do conjunto da sociedade, estar?o se arriscando a evoluir para uma situa??o de atividade apenas virtual.
O desafio da malária: o caso brasileiro e o que se pode esperar dos progressos da era gen?mica
Silva,Luiz Hildebrando Pereira da; Oliveira,Vera Engracia Gama de;
Ciência & Saúde Coletiva , 2002, DOI: 10.1590/S1413-81232002000100005
Abstract: malaria endemic areas in brazil are restricted to the amazon region, with an average of 500 thousand new cases every year. the situation can be defined as unstable hipoendemic with, however, foci of high endemicity. demographic and socio economic factors are main determinants in the malaria challenge for the public health system. in the present paper, biological and social factors responsible for the unstable endemic situation are discussed. the need for a permanent surveillance and intervention of public health services are stressed to avoid the occurrence of local epidemics and spreading of endemic areas. in the paper, are also summarised recent lines of research developed in the post genomic era in the studies of parasite, vector and human molecular genetics that would favour the development, in the future, of new tools and procedures for malaria control
O desafio da malária: o caso brasileiro e o que se pode esperar dos progressos da era gen mica
Silva Luiz Hildebrando Pereira da,Oliveira Vera Engracia Gama de
Ciência & Saúde Coletiva , 2002,
Abstract: A área endêmica de malária no Brasil se estende atualmente à totalidade da regi o amaz nica, com cerca de 500 mil casos anuais, em geral com situa es de baixa e média endemicidade mas ainda apresentando focos de alto risco. Fatores demográficos e socioecon micos s o dominantes nos desafios que enfrentam os Servi os de Saúde Pública no controle da malária. No presente artigo s o discutidos fatores determinantes da instabilidade da situa o endêmica bem como a necessidade de a es permanentes de vigilancia e de interven o dos Servi os de Saúde para que se evitem surtos epidêmicos e alastramento das áreas endêmicas. No artigo, em seguida, apresenta-se uma síntese de progressos recentes nos estudos da era gen mica e pós-gen mica sobre o parasita, o vetor e o hospedeiro humano que podem favorecer, no futuro, o desenvolvimento e a melhoria dos métodos de controle da malária.
Endemias e epidemias na Amaz?nia: malária e doen?as emergentes em áreas ribeirinhas do Rio Madeira. Um caso de escola
Katsuragawa, Tony Hiroshi;Gil, Luiz Herman Soares;Tada, Mauro Shugiro;Silva, Luiz Hildebrando Pereira da;
Estudos Avan?ados , 2008, DOI: 10.1590/S0103-40142008000300008
Abstract: the authors present a short review on the evolution of malaria incidence in the brazilian amazon, with particular reference to the rond?nia state and the municipality of porto velho, where dramatic epidemics of malaria and other tropical diseases have been registered in the past. next, they analyze the present endemic malaria situation in the madeira river valley where two important hydroelectric power plants will be constructed in the localities of santo ant?nio and jirau. longitudinal surveys performed in the last four years in this area allow to demonstrate a high prevalence of symptomatic and asymptomatic malaria carriers in the area, associated to the presence of high densities of the malaria vector anopheles darlingi all over the year. these elements are correlated to the expected arrival of a large number of human migrants originated from non endemic areas of rond?nia and other brazilian states, attracted by the possibility of jobs in the hydroelectric power plants and by secondary opportunities in commerce, leisure, education and domestic activities. these associations create favorable conditions for malaria outbreaks and other tropical diseases that must be avoided by the establishment of additional control measures, in particular in the sanitation domain
Urban and suburban malaria in Rond?nia (Brazilian Western Amazon) II: perennial transmissions with high anopheline densities are associated with human environmental changes
Gil, Luiz Herman Soares;Tada, Mauro Shugiro;Katsuragawa, Tony Hiroshi;Ribolla, Paulo Eduardo Martins;Silva, Luiz Hildebrando Pereira da;
Memórias do Instituto Oswaldo Cruz , 2007, DOI: 10.1590/S0074-02762007005000013
Abstract: longitudinal entomological surveys were performed in vila candelária and adjacent rural locality of bate estaca concomitantly with a clinical epidemiologic malaria survey. vila candelária is a riverside periurban neighborhood of porto velho, capital of the state of rond?nia in the brazilian amazon. high anopheline densities were found accompanying the peak of rainfall, as reported in rural areas of the region. moreover, several minor peaks of anophelines were recorded between the end of the dry season and the beginning of the next rainy season. these secondary peaks were related to permanent anopheline breeding sites resulting from human activities. malaria transmission is, therefore, observed all over the year. in vila candelária, the risk of malaria infection both indoors and outdoors was calculated as being 2 and 10/infecting bites per year per inhabitant respectively. urban malaria in riverside areas was associated with two factors: (1) high prevalence of asymptomatic carriers in a stable human population and (2) high anopheline densities related to human environmental changes. this association is probably found in other amazonian urban and suburban communities. the implementation of control measures should include environmental sanitation and better characterization of the role of asymptomatic carriers in malaria transmission.
Microsatellite characterization of Plasmodium falciparum from symptomatic and non-symptomatic infections from the Western Amazon reveals the existence of non-symptomatic infection-associated genotypes
Martha, Rosimeire Cristina Dalla;Tada, Mauro Sughiro;Ferreira, Ricardo Godoi de Mattos;Silva, Luiz Hildebrando Pereira da;Wunderlich, Gerhard;
Memórias do Instituto Oswaldo Cruz , 2007, DOI: 10.1590/S0074-02762007005000044
Abstract: in western amazon areas with perennial malaria transmission, long term residents frequently develop partial immunity to malarial infection caused either by plasmodium falciparum or p. vivax, resulting in a considerable number of non-symptomatically infected individuals. for yet unknown reasons, these individuals sporadically develop symptomatic malaria. in order to identify if determined parasite genotypes, defined by a combination of eleven microsatellite markers, were associated to different outcomes - symptomatic or asymptomatic malaria - we analyzed infecting p. falciparum parasites in a suburban riverine population. despite of detecting a high degree of diversity in the analyzed samples, several microsatellite marker alleles appeared accumulated in parasites from non-symptomatic infections. this result may be interpreted that a number of microsatellites, which are not directly related to antigenic features, could be associated to the outcome of malarial infection. the result may also point to a low frequency of recombinatorial events which otherwise would dissociate genes under strong immune pressure from the relatively neutral microsatellite loci.
Enzyme Mechanism and Slow-Onset Inhibition of Plasmodium falciparum Enoyl-Acyl Carrier Protein Reductase by an Inorganic Complex
Patrícia Soares de Maria de Medeiros,Rodrigo Gay Ducati,Luiz Augusto Basso,Diógenes Santiago Santos,Luiz Hildebrando Pereira da Silva
Enzyme Research , 2011, DOI: 10.4061/2011/642758
Abstract: Malaria continues to be a major cause of children's morbidity and mortality worldwide, causing nearly one million deaths annually. The human malaria parasite, Plasmodium falciparum, synthesizes fatty acids employing the Type II fatty acid biosynthesis system (FAS II), unlike humans that rely on the Type I (FAS I) pathway. The FAS II system elongates acyl fatty acid precursors of the cell membrane in Plasmodium. Enoyl reductase (ENR) enzyme is a member of the FAS II system. Here we present steady-state kinetics, pre-steady-state kinetics, and equilibrium fluorescence spectroscopy data that allowed proposal of P. falciparum ENR (PfENR) enzyme mechanism. Moreover, building on previous results, the present study also evaluates the PfENR inhibition by the pentacyano(isoniazid)ferrateII compound. This inorganic complex represents a new class of lead compounds for the development of antimalarial agents focused on the inhibition of PfENR.
Page 1 /82306
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.