oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2020 ( 1 )

2019 ( 18 )

2018 ( 41 )

2017 ( 25 )

Custom range...

Search Results: 1 - 10 of 22821 matches for " Luiz Filipe Protasio;Coutinho "
All listed articles are free for downloading (OA Articles)
Page 1 /22821
Display every page Item
Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation
Ribas, Alessandra Ferreira;Kobayashi, Adilson Kenji;Pereira, Luiz Filipe Protasio;Vieira, Luiz Gonzaga Esteves;
Brazilian Archives of Biology and Technology , 2006, DOI: 10.1590/S1516-89132006000100002
Abstract: transgenic plants of coffea canephora p. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with agrobacterium tumefaciens strain eha105 harboring pcambia3301, a plasmid that contains the bar and the uida genes both under control of 35s promoter. direct somatic embryogenesis was induced on basal medium contained ? strength macro salts and half strength micro salts of ms medium, organic constituents of b5 medium and 30 g.l-1 sucrose supplemented with 5μm n6 - (2-isopentenyl)-adenine (2-ip). ten μm ammonium glufosinate was used for putative transgenic somatic embryos selection. presence and integration of the bar gene were confirmed by pcr and southern blot analysis. selected transgenic coffee plants sprayed with up to 1600 mg.l-1 of finale?, a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.
Genetic transformation of coffee
Ribas, Alessandra Ferreira;Pereira, Luiz Filipe Protasio;Vieira, Luiz Gonzaga E.;
Brazilian Journal of Plant Physiology , 2006, DOI: 10.1590/S1677-04202006000100007
Abstract: in the last 15 years, considerable advances were made in coffee genetic transformation. different research groups in the world have been able to transform coffee with genes for insect resistance, decaffeinated coffee, herbicide resistance and control of fruit maturation. although the majority of the research is still limited to laboratory and greenhouse studies, initial field tests with transformed coffee are beginning to appear in the literature. in this review we provide an update on the state of coffee genetic transformation, presenting technical aspects related to tissue culture systems, strategies for selection and transformation with particle bombardment, as well as the use of agrobacterium tumefaciens. we also discuss the potential applications of this technology, taking into consideration the benefits, the possible environmental risks, as well as market and consumer issues.
Ethylene production and acc oxidase gene expression during fruit ripening of Coffea arabica L.
Pereira, Luiz Filipe Protasio;Galv?o, Rafaelo M.;Kobayashi, Adilson K.;Ca??o, Sandra Maria B.;Vieira, Luiz Gonzaga Esteves;
Brazilian Journal of Plant Physiology , 2005, DOI: 10.1590/S1677-04202005000300002
Abstract: the phytohormone ethylene is involved in several physiological and developmental processes in higher plants, including ripening of fruits, abscission of organs and tissues, senescence, wound response as well as in other abiotic stresses. the enzyme 1-aminocyclopropane-1-carboxylate oxidase (aco) catalyzes the last step of ethylene biosynthesis. the production of ethylene and the expression of a coffea arabica aco gene during the last stages of fruit maturation were investigated. a rapid increase of ethylene production at the green-yellow stage of fruit ripening, after the end of endosperm formation, and a decrease of ethylene production at the cherry stage indicates a climacteric phase during ripening. an acc oxidase (ca-aco) from coffee fruit cdna was cloned and characterized using primers previously reported. the cdna is homologous to previously described acc oxidase cdna in coffea. the nucleotide and amino acid deduced sequences of the clone showed high homology with aco from climacteric fruits. northern blots were performed to determine the ca-aco transcription pattern from different tissues and from fruits at different ripening stages. coffee fruits at an early ripening stage (green) showed the lowest level of ca-aco transcript accumulation. the transcript levels of ca-aco did not change significantly during the later stages, suggesting the presence of post- transcriptional control mechanisms. these results, taken together, strongly suggest a climacteric nature of coffee fruit ripening.
Produ??o de prolina e suscetibilidade ao glufosinato de am?nio em plantas transgênicas de citrumelo Swingle
Carneiro, Cristine Elizabeth Alvarenga;Molinari, Hugo Bruno Correa;Andrade, Giselly Aparecida;Pereira, Luiz Filipe Protasio;Vieira, Luiz Gonzaga Esteves;
Pesquisa Agropecuária Brasileira , 2006, DOI: 10.1590/S0100-204X2006000500005
Abstract: the objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of swingle citrumelo with high proline production. the mutant gene of the enzyme d1-pyrroline-5-carboxylate synthetase (p5cs), the rate-limiting enzyme in proline biosynthesis, was inserted into swingle citrumelo plants. the expression of the gene p5cs caused up to 5-fold increase on the proline content in leaf tissues of transgenic plants treated with 200 μm glufosinate ammonium, when compared with control plants. leaves of transgenic plants accumulated higher amounts of nh4+ than the nontransgenic control. the herbicide toxicity was evaluated using leaf disks cultivated in ms medium, containing different concentrations of glufosinate ammonium. the severity of the chlorosis, observed in leaf disks of transgenic plants, confirmed the higher susceptibility of swingle citrumelo plants, with high proline production, to this herbicide.
Brazilian coffee genome project: an EST-based genomic resource
Vieira, Luiz Gonzaga Esteves;Andrade, Alan Carvalho;Colombo, Carlos Augusto;Moraes, Ana Heloneida de Araújo;Metha, ?ngela;Oliveira, Angélica Carvalho de;Labate, Carlos Alberto;Marino, Celso Luis;Monteiro-Vitorello, Claúdia de Barros;Monte, Damares de Castro;Giglioti, éder;Kimura, Edna Teruko;Romano, Eduardo;Kuramae, Eiko Eurya;Lemos, Eliana Gertrudes Macedo;Almeida, Elionor Rita Pereira de;Jorge, érika C.;Albuquerque, érika V. S.;Silva, Felipe Rodrigues da;Vinecky, Felipe;Sawazaki, Haiko Enok;Dorry, Hamza Fahmi A.;Carrer, Helaine;Abreu, Ilka Nacif;Batista, Jo?o A. N.;Teixeira, Jo?o Batista;Kitajima, Jo?o Paulo;Xavier, Karem Guimar?es;Lima, Liziane Maria de;Camargo, Luis Eduardo Aranha de;Pereira, Luiz Filipe Protasio;Coutinho, Luiz Lehmann;Lemos, Manoel Victor Franco;Romano, Marcelo Ribeiro;Machado, Marcos Antonio;Costa, Marcos Mota do Carmo;Sá, Maria Fátima Grossi de;Goldman, Maria Helena S.;Ferro, Maria Inês T.;Tinoco, Maria Laine Penha;Oliveira, Mariana C.;Van Sluys, Marie-Anne;Shimizu, Milton Massao;Maluf, Mirian Perez;Eira, Mirian Therezinha Souza da;Guerreiro Filho, Oliveiro;Arruda, Paulo;Mazzafera, Paulo;Mariani, Pilar Drummond Sampaio Correa;Oliveira, Regina L.B.C. de;Harakava, Ricardo;Balbao, Silvia Filippi;Tsai, Siu Mui;Mauro, Sonia Marli Zingaretti di;Santos, Suzana Neiva;Siqueira, Walter José;Costa, Gustavo Gilson Lacerda;Formighieri, Eduardo Fernandes;Carazzolle, Marcelo Falsarella;Pereira, Gon?alo Amarante Guimar?es;
Brazilian Journal of Plant Physiology , 2006, DOI: 10.1590/S1677-04202006000100008
Abstract: coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. the genus coffea belongs to the rubiaceae family which includes other important plants. the genus contains about 100 species but commercial production is based only on two species, coffea arabica and coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. the brazilian coffee genome project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. we have single-pass sequenced a total of 214,964 randomly picked clones from 37 cdna libraries of c. arabica, c. canephora and c. racemosa, representing specific stages of cells and plant development that after trimming resulted in 130,792, 12,381 and 10,566 sequences for each species, respectively. the ests clustered into 17,982 clusters and 32,155 singletons. blast analysis of these sequences revealed that 22 % had no significant matches to sequences in the national center for biotechnology information database (of known or unknown function). the generated coffee est database resulted in the identification of close to 33,000 different unigenes. annotated sequencing results have been stored in an online database at http://www.lge.ibi.unicamp.br/cafe. resources developed in this project provide genetic and genomic tools that may hold the key to the sustainability, competitiveness and future viability of the coffee industry in local and international markets.
Aproveitamento da cinza do baga o de cana-de a úcar como fíler em concreto asfáltico
Cláudio Luiz Dias Leal,Protasio Ferreira e Castro
Vértices , 2010,
Abstract: A gera o, o manuseio e o armazenamento seguro de resíduos sólidos têm se tornado uma grande preocupa o no Brasil. O principal objetivo desse estudo foi investigar a potencialidade do uso da cinza do baga o de cana de a úcar como fíler em misturas asfálticas. O comportamento das misturas foi caracterizado por meio de um programa experimental utilizando diferentes teores de resíduo e os ensaios de estabilidade Marshall, tra o indireta, módulo de resiliência e velocidade do pulso ultra-s nico. A análise estatística aplicada aos resultados dos ensaios mostrou que a cinza do baga o de cana pode ser usada como fíler em misturas betuminosas. Applying sugar cane pulp ashes as filler to asphalt concrete The generation, handling and safe disposal of solid wastes has become a major concern in Brazil. The primary objective of this study was to investigate the feasibility of using sugar cane pulp ashes as filler to asphalt mixtures. The mixture’s behavior was characterized through an experimental program consisting of different industrial waste content and several test methods: Marshall stability, diametral indirect tensile test, resilient modulus and ultrasonic pulse velocity. Statistical analyses applied to the test results showed that sugar cane pulp ashes can be used as filler in bituminous mixes.
Knowledge and concealment: the historiographical discourse on euro-brazilian colonization and ethnic otherness in southwest Paraná (Brazil)
Protasio Langer
Diálogos , 2010, DOI: 10.4025/dialogos.v11i3.53
Abstract: The present article proposes an analysis of the historiographical speech on the native people who, during the 1040s and 1050s, were confronted by the flow of southern colonization, in southwestern Paraná. We will analyze, therefore, the clichés and the postulates that permeate academic knowledge and are corroborated by the hiding and depreciation of the indigenous groups and caboclos involved in the intense transformations caused by the expansion in colonial space by incoming Euro-Brazilian families from the states of Rio Grande do Sul and Santa Catarina. El presente artículo propone un análisis del discurso historiográfico sobre las etnias nativas del Suroeste del Estado de Paraná que, en las décadas de 1940 y 1950, se depararon con el flujo de colonización oriunda del Sur del Brasil. Por lo tanto, analizaremos los postulados e ideas perogrulladas que envuelven el conocimiento académico y que corroboran el ocultamiento y el desprecio de grupos indígenas y de caboclos, involucrados en las intensas transformaciones ocurridas con la expansión del espacio colonial, promovida por familias euro-brasile as, oriundas de Río Grande do Sul y de Santa Catarina. O presente artigo prop e uma análise do discurso historiográfico sobre as etnias nativas que, nas décadas de 40 e 50 do século XX, se defrontaram com o fluxo de coloniza o sulista no Sudoeste do Paraná. Analisaremos, portanto, os clichês e os postulados que permeiam o conhecimento acadêmico e corroboram o ocultamento e a deprecia o de grupos indígenas e de caboclos envolvidos nas intensas transforma es instauradas pela expans o do espa o colonial movido por famílias eurobrasileiras procedentes do Rio Grande do Sul e de Santa Catarina.
Determina??o do coeficiente de atividade em dilui??o infinita de hidrocarbonetos em furfural a 298,15 K por SPME-GC/FID
Furtado, Filipe Arantes;Coelho, Gerson Luiz Vieira;
Química Nova , 2010, DOI: 10.1590/S0100-40422010000900016
Abstract: in this work a new method (spme-gc/fid) was developed to analyze the activity of binary liquid mixtures. the purpose is to demonstrate that spme is capable to be used to determinate activity coefficients at infinite dilution knowing the fiber properties, with a lower cost than the conventional methods encountered in literature such as glc. the activity coefficients at infinite dilution in furfural for n-hexane, n-heptane and cyclohexane at 298.15 k was determined using spme and deviations of literature data was about 7%.
O APROVEITAMENTO DA CINZA DE CALDEIRA NA CONSTRU O CIVIL
Cláudia Barroso Vasconcellos,Cláudio Luiz Dias Leal,Marcelo Pereira Fran?a,Protasio Ferreira e Castro
Vértices , 2010,
Abstract: Esse trabalho apresenta um estudo do potencial de aproveitamento de cinza de caldeira. No Brasil, o volume desse resíduo industrial tem aumentado significativamente devido à implementa o da política de fontes geradoras de energia elétrica, que enfatiza o uso de usinas termelétricas e mais especificamente a aplica o do gás natural. O programa experimental foi desenvolvido por meio da aplica o de cinza em misturas asfálticas e em argamassas de cimento Portland. A análise estatística dos resultados dos ensaios permite afirmar que a cinza de caldeira possui potencial para ser aplicada em misturas de cimento asfáltico de petróleo e de cimento Portland.
Comparison of seedling growth among three co-occurring varieties of Araucaria angustifolia (Bertol.) Kuntze under greenhouse conditions
Coutinho, André Luiz;Dillenburg, Lúcia Rebello;
Acta Botanica Brasilica , 2010, DOI: 10.1590/S0102-33062010000200025
Abstract: araucaria angustifolia (bertol.) kuntze is a tall, long-lived tree species, which grows as an emergent tree in the araucaria forests of southern brazil. four varieties have been described for this species, and three of them were selected for this study: "angustifolia" (the type variety), "caiova" and "indehiscens". these are co-occurring varieties that differ in external seed color and timing of seed maturation. the purpose of this study was to compare the initial growth of plants originated from seeds of these three varieties, in order to test the hypothesis that these varieties also differ in the initial growth of their seedlings. seeds were collected from a single forest location, and 60 plants per variety were grown in pots for a period of 244 days. the "angustifolia" variety accumulated significantly more mass (both in shoot and root) than the other two, but the three varieties did not differ in mass allocation between shoot and root and between lateral roots and the main root. the greater growth of the type variety may help explain its greater natural abundance, since attaining a larger size will have positive effects on seedling recruitment and survival.
Page 1 /22821
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.