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Search Results: 1 - 10 of 22055 matches for " Luiz Filipe Damé;Meireles "
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Evaluación de la actividad bactericida de aceites esenciales de hojas de guayabo, pitango y arazá
de Souza Prestes,Luciana; Damé Schuch,Luiz Filipe; H?rnke Alves,Gabriela; Ziemann dos Santos,Marco Aurélio; Alves Rodrigues,Maria Regina; Araújo Meireles,Mario Carlos;
Revista Cubana de Plantas Medicinales , 2011,
Abstract: introduction: guava, surinam cherry and strawberry guava are native south american fruit trees and the tea from their leaves is traditionally used to treat diarrhea. objective: to evaluate the antimicrobial action of psidium guajava l., eugenia uniflora l. and psidium cattleyanum sabine and their constituents. methods: the essential oils were extracted by a modified clevenger. the analysis of essential oils was performed on chromatograph (gc/ms). the antimicrobial activity of obtained oils against escherichia coli, salmonella typhimurium and staphylococcus aureus was evaluated by broth microdilution assay to determine their minimum bactericidal concentration. results: the guava oil showed bactericidal activity against e. coli at 2 % and 8 % concentration rates against s. aureus and s. typhimurium. the strawberry guava oil showed no bactericidal activity against tested microorganisms whereas surinam cherry had bactericidal activity at 8 % concentration against s. aureus and s. typhimurium. the main constituents commonly found in the three plant oils were a-copaene and a-humulene. conclusions: the oils showed bactericidal activity against tested microorganisms and the constituents are comparable to those found in other studies.
CLSI broth microdilution method for testing susceptibility of Malasseziapachydermatis to thiabendazole
Nascente, Patrícia da Silva;Meinerz, Ana Raquel Mano;Faria, Renata Osório de;Schuch, Luiz Filipe Damé;Meireles, Mário Carlos Araújo;Mello, Jo?o Roberto Braga de;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000200002
Abstract: thiabendazole, classified as antiparasitic and also used as an antifungal drug, can be found as otological solution indicated for treatment of parasitic and fungal external otitis in small animals. malassezia pachydermatis is a yeast recognized as a normal inhabitant on the skin and mucous membranes of dogs and cats. however, it is considered an opportunistic agent that causes external otitis and dermatitis in these animals. the aim of this study was to evaluate the in vitro effect of thiabendazole against 51 isolates of m. pachydermatis using the clsi broth microdilution method that has been adapted for this yeast species (nccls, 2002). based on this test, the minimum inhibitory concentrations (mic) of thiabendazol was calculated. subsequently, the susceptibility of each isolate against this antifungal was determined. it was observed that the mic of thiabendazole against m. pachydermatis ranged from 0.03 to > 4 μg/ml. a total of 13.7% of the isolates were found to be resistant, 47.1% were intermediate and 39.2% were sensitive to the drug. the rate of resistance of the yeasts against thiabendazole was similar to the results previously obtained with other antifungals, while the adapted broth microdilution technique used in this study proved to be efficient.
Efeitos de doses elevadas da terbinafina e itraconazol em ratos Wistar
Meinerz, Ana Raquel Mano;Cleff, Marlete Brum;Nascente, Patrícia da Silva;Nobre, Márcia de Oliveira;Schuch, Luiz Filipe Damé;Antunes, Tatiana de ávila;Xavier, Melissa O.;Meireles, Mário Carlos Araújo;Mello, Jo?o Roberto de Braga;
Revista Brasileira de Ciências Farmacêuticas , 2007, DOI: 10.1590/S1516-93322007000100013
Abstract: taking into account the importance of the itraconazole and terbinafine in the antifungal therapy, the study has as objective evaluate the effects of high itraconazole doses (100 mg/kg) and terbinafine (250 mg/kg) in rats albino wistar through the analyses of the hepatic enzymes (alt and alp), complete blood count and histopathologic study of different organs. the drugs were administered orally once a day, for a period of 30 days, when blood was collected and accomplished the necropsy of the experimental animals. the detected values of the hepatic enzymes and of the blood count were compatible to the physiologic indexes for the studied species with no statistical differences among the experimental groups. the histopathologic exam did not reveal any abnormality, however 25% of the treated with terbinafine died immediately after the administration of the drug. with those results we concluded that the administered doses of the drugs did not alter the appraised hepatic enzymes, as well as the blood count. however, more studies are needed to consider high doses of the terbinafine and itraconazole as viable treatment alternative for systemic mycosis.
Suscetibilidade in vitro de isolados de Sporothrix schenckii frente à terbinafina e itraconazol
Meinerz, Ana Raquel Mano;Nascente, Patrícia da Silva;Schuch, Luiz Filipe Damé;Cleff, Marlete Brum;Santin, Rosema;Brum, Cristiane da Silva;Nobre, Márcia de Oliveira;Meireles, Mario Carlos Araújo;Mello, Jo?o Roberto de Braga;
Revista da Sociedade Brasileira de Medicina Tropical , 2007, DOI: 10.1590/S0037-86822007000100012
Abstract: the study objective was to determine the in vitro activity of terbinafine and itraconazole through the microdilution technique in broth (ncclsm27-a2), adapted for dimorphic fungus, in relation to 12 isolates of sporothrix schenckii. six were from feline sporotrichosis, three from human sporotrichosis, one from a dog and two from human isolates originating from instituto oswaldo cruz. the inoculum and antifungal concentrates were distributed on microplates that were incubated at 35°c for five days. minimum inhibitory concentration readings were made at the end of this period. the mic for terbinafine ranged from 0.055μg/ml to 0.109μg/ml, and the mic for itraconazole ranged from 0.219μg/ml to 1.75μg/ml. for both drugs, the mic from the isolates from ioc was 0.875μg/ml. the present study demonstrates the high susceptibility of sporothrix schenckii to terbinafine. further studies to correlate the in vitro susceptibility tests with the clinical response of patients with sporotrichosis are needed.
Actividad de extractos de orégano y tomillo frente a microorganismos asociados con otitis externa
de Souza Prestes,Luciana; Frascolla,Ricardo; Santin,Rosema; Ziemann dos Santos,Marco Aurelio; Costa Schram,Renata; Alves Rodrigues,Maria Regina; Damé Schuch,Luiz Filipe; Araújo Meireles,Mario Carlos;
Revista Cubana de Plantas Medicinales , 2008,
Abstract: introduction: the use of plants for medicinal purposes to treat diseases has been seen throughout the history of mankind. the most common diagnosed disease in dogs is external otitis, and therefore, the clinical importance of this illness is great. objetive: to evaluate the in vitro antimicrobial activity of 3 types of extracts from origanum vulgare l. (oregano) and thymus vulgaris l. (thyme) in the form of an oil, a tincture and a decoction against otitis-associated microorganisms. methods: the evaluation of these extracts was performed through microditulion in broth against malassezia pachydermatis, pseudomonas aeruginosa (atcc 10145) y staphylococcus aureus (atcc 12600) isolates. the oil components were compared by chromatography technique. results: the oils presented with a lower minimum inhibitory concentration against microorganisms, but the best performance extracts were tinctures. conclusions: these two types of extracts may be an alternative for the treatment of external otitis in dogs.
Comparación de distintas extracciones hidroalcohólicas de plantas con indicativo etnográfico antiséptico/desinfectante
Voigt Mota,Fernanda; Lambrecht Gon?alves,Carolina; Damé Schuch,Luiz Filipe; Silveira Coimbra,Helen; Hartwig,Carla;
Revista Cubana de Plantas Medicinales , 2011,
Abstract: introduction: the use of low risk natural products in the animal production has acquired growing importance in the last few years. objectives: to compare the antibacterial action of several hydroalcoholic extracts from 4 plants (baccharis trimera [less] dc, bidens pilosa l., eucalyptus sp. and tagetes minuta l.) against 2 microorganisms causing mastitis (staphylococcus aureus and streptococcus agalactia). methods: extracts from fresh leaves using 92.8°alcohol and from dry leaves using 70° and 50° alcohol were obtained. the antibacterial action of each extract was measured on the basis of the capacity of inactivating 105-106 cfu/ml of each bacterium at 3 contacting times of 30 s, 5 min and 20 min. the turkev′s assay-based variation and comparison analysis of the means provided the final outcomes. results: all the extracts had some antibacterial effect. the extract from t. minuta dry leaves using 70° alcohol showed the biggest effect (p< 0,05) whereas the extract from eucalyptus spp. fresh leaves proved to be more effective, being the difference significant. the other 2 plants did not show any difference in terms of antibacterial effect for the various forms of obtaining the extract. conclusions: the antibacterial action is greater with dry leaf extract from t. minuta and fresh leaf extract from eucalyptus spp.
Aplicación de plantas medicinales para la antisepsia de pezones de vacas posorde o Use of medicinal plants for antisepsis of cows' teat after milking
Diane Bender Almeida Schiavon,Luiz Filipe Damé Schuch,Marta Elaine Bastos Oyarzabal,Luciana de Souza Prestes
Revista Cubana de Plantas Medicinales , 2011,
Abstract: Introducción: el sistema de producción de leche agroecológico está limitado por alternativas sostenibles de promoción a la salud animal. El control de la mastitis con plantas medicinales puede ser viable de modo económico y ecológicamente. Objetivos: evaluar la aplicación de antisépticos en la desinfección de pezones posorde o. Métodos: se comparó un convencional y extracto de plantas medicinales en un reba o comercial y tuvo como principal encierre las nuevas infecciones intramamarias. Resultados: la prevalencia semanal del California Mastitis Test varió en el grupo 1 entre 29,5 y 17,1 %, y en el grupo 2 de 29,7 a 19,6 %, no diferenciándose de modo significativo, en ninguna de las semanas. La incidencia de cultura positiva para Staphylococcus/Streptococcus fue de 3,93 y 6,96/1 000 cuartos/d para los grupos 1 y 2, respectivamente; con p= 0,057. Hubo 4 casos de mastitis clínica durante el experimento, 2 en cada tratamiento. Conclusiones: el uso de extractos de plantas en la desinfección de pezones posorde o puede ser útil a los sistemas de producción de leche agroecológicos. Introduction: the agro-ecological system of milk production is limited in terms of sustainable alternatives for animal health promotion. The control of mastitis with medicinal plants can be economically and environmentally viable. Objectives: to evaluate the use of antiseptics in the post-milking teat disinfection. Methods: the comparison of conventional and herbal extracts in treating the new intramammarial infections. Results: the weekly prevalence of California Mastitis Test ranged from 29.5 to 17.1 % in group 1 and from 29.7 to 19.6 % in group 2 but no significant difference was found in any week. The incidence of positive culture for Staphylococcus/Streptococcus was 3.93 and 6.96/1 000 quarters/day for groups 1 and 2 respectively, being p= 0.057. There were four cases of clinical mastitis during the experiment, two cases in each treatment. Conclusions: the use of plant extracts in the post-milking teat disinfection may be useful for agro-ecological systems of milk production.
Actividad antimicrobiana de aceite esencial de Origanum vulgare L. ante bacterias aisladas en leche de bovino Antimicrobial action of Origanum vulgare L. essential oil against bacteria isolated from bovine milk
Marta Elaine Bastos Oyarzabal,Luiz Filipe Damé Schuch,Luciana de Souza Prestes,Diane Bender Almeida Schiavon
Revista Cubana de Plantas Medicinales , 2011,
Abstract: Introducción: la mastitis bovina es el mayor problema en la producción lechera, causada principalmente por bacterias grampositivas. La búsqueda de principios activos que actúen en esos microrganismos es creciente, sobre todo por la ocurrencia de multirresistencia bacteriana. Objetivos: evaluar la concentración bactericida mínima del aceite esencial de Origanum vulgare L. (orégano) frente a bacterias aisladas de leche mastítica. Métodos: se evaluó la actividad antimicrobiana del aceite esencial de O. vulgare frente a 71 bacterias aisladas de leche bovina, de los géneros Streptococcus, Staphylococcus y Corynebacterium; y 3 cepas patrón de Pseudomonas aeruginosa, Staphylococcus aureus y Escherichia coli. La técnica utilizada fue de dilución en microplaca. Resultados: la concentración bactericida mínima media varió de 0,23 a 2 % frente a las bacterias aisladas de leche bovina, con la menor concentración para el género Streptococcus y la mayor para Staphylococcus coagulasa negativa. En cuanto a las cepas patrones la concentración bactericida mínima fue de 3,17 y 0,35 % para S. aureus y Escherichia coli, respectivamente; no presentó efecto para Pseudomonas aeruginosa. Conclusiones: en los resultados se comprobó la actividad in vitro del aceite de orégano frente a las bacterias relacionadas con la mastitis bovina. Introduction: bovine mastitis is a major problem in dairy production, mainly caused by Gram-positive bacteria. The search for active ingredients that act upon these microorganisms is growing due to occurrence of bacterial multiresistance. Objectives: to assess the minimum bactericidal concentration (MBC) of Origanum vulgare L. (oregano) essential oil against bacteria isolated from mastitic milk. Methods: the antimicrobial activity of O. vulgare essential oil was measured against 71 bacteria from the genera Streptococcus, Staphylococcus and Corynebacterium isolated from the bovine milk and against three pattern strains, that is, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The technique was dilution in microplate. Results: the mean minimal bactericidal concentration (MBC) ranged from 0.23 % to 2 % against the bacteria isolated from bovine milk, with the lowest concentration for Streptococcus genus and the highest for coagulase-negative Staphylococcus. Regarding the pattern strains, the MBC was 3.17 % and 0.35 % for S. aureus and Escherichia coli respectively and showed no effect for Pseudomonas aeruginosa. Conclusions: the results confirmed the in vitro activity of Origanum vulgare L. oil against the bovine mastitis-related bate
Comparación de distintas extracciones hidroalcohólicas de plantas con indicativo etnográfico antiséptico/desinfectante Comparison of several hydroalcoholic extracts from plants with antiseptic/disinfectant ethnographic indication
Fernanda Voigt Mota,Carolina Lambrecht Gon?alves,Luiz Filipe Damé Schuch,Helen Silveira Coimbra
Revista Cubana de Plantas Medicinales , 2011,
Abstract: Introducción: la utilización de productos naturales y de bajo riesgo en la producción animal ha alcanzado elevada importancia en los últimos a os. Objetivos: comparar la actividad antibacteriana de diferentes extractos hidroalcohólicos de 4 plantas (Baccharis trimera [Less] DC, Bidens pilosa L., Eucalyptus sp. y Tagetes minuta L.) frente a 2 microorganismos que causan mastitis (Staphylococcus aureus y Streptococcus agalactia). Métodos: se obtuvieron extractos de hojas frescas con alcohol a 92,8° y de hojas secas con alcohol a 70 y 50°. La actividad antibacteriana de cada extracto se midió por la capacidad de inactivación de 10(5)-10(6) UFC/mL de cada bacteria, en 3 tiempos de contacto, 30 s, 5 y 20 min. Los resultados obtenidos se hicieron por análisis de variación y comparación de las medias utilizando el ensayo de Turkey. Resultados: se obtuvo efecto antibacteriano con los extractos de todas las plantas. Para T. minuta, el extracto de hoja seca con alcohol de 70° fue el efecto mayor (p< 0,05), mientras que para Eucalyptus spp., se encontró que el extracto de hoja fresca resultó más eficaz, con diferencia significativa. Las otras 2 plantas no presentaron diferencia de efecto antibacteriano para las distintas formas de obtención de extracto. Conclusiones: hay mayor actividad antibacteriana, cuando el extracto de T. minuta es obtenido de hojas secas y en Eucalyptus spp. de hojas frescas. Introduction: the use of low risk natural products in the animal production has acquired growing importance in the last few years. Objectives: to compare the antibacterial action of several hydroalcoholic extracts from 4 plants (Baccharis trimera [Less] DC, Bidens pilosa L., Eucalyptus sp. and Tagetes minuta L.) against 2 microorganisms causing mastitis (Staphylococcus aureus and Streptococcus agalactia). Methods: extracts from fresh leaves using 92.8°alcohol and from dry leaves using 70° and 50° alcohol were obtained. The antibacterial action of each extract was measured on the basis of the capacity of inactivating 10(5)-10(6) CFU/ml of each bacterium at 3 contacting times of 30 s, 5 min and 20 min. The Turkev′s assay-based variation and comparison analysis of the means provided the final outcomes. Results: all the extracts had some antibacterial effect. The extract from T. minuta dry leaves using 70° alcohol showed the biggest effect (p< 0,05) whereas the extract from Eucalyptus spp. fresh leaves proved to be more effective, being the difference significant. The other 2 plants did not show any difference in terms of antibacterial effect for the various forms of obtaini
Evaluación de la actividad bactericida de aceites esenciales de hojas de guayabo, pitango y arazá Evaluation of the bactericidal action of essential oils from guava, Surinam cherry and strawberry guava
Luciana de Souza Prestes,Luiz Filipe Damé Schuch,Gabriela H?rnke Alves,Marco Aurélio Ziemann dos Santos
Revista Cubana de Plantas Medicinales , 2011,
Abstract: Introducción: guayabo, pitango y arazá son árboles fructíferos, nativos de América del Sur. El té de sus hojas es utilizado popularmente en el tratamiento de la diarrea. Objetivo: evaluar la actividad antimicrobiana de los aceites de las hojas Psidium guajava L., Eugenia uniflora L. y Psidium cattleyanum Sabine y sus constituyentes. Métodos: los aceites esenciales fueron extraídos con un aparato tipo Clevenger modificado. Su análisis se realizó en cromatógrafo (GC/MS). Se evaluó la actividad antimicrobiana de los aceites obtenidos frente a Escherichia coli, Salmonella typhimurium y Staphylococcus aureus, mediante la técnica de microdilución en caldo con determinación de la concentración bactericida mínima del aceite. Resultados: el aceite del guayabo presentó actividad bactericida frente a E. coli en la concentración de 2 y 8 % frente al Staphylococcus aureus y Salmonella typhimurium. El aceite de arazá no presentó actividad bactericida frente a los microorganismos testados y el aceite de pitango presentó actividad bactericida en la concentración de 8 % frente al Staphylococcus aureus y Salmonella typhimurium. Los constituyentes principales encontrados en común en los aceites de las 3 plantas fueron el a-copaeno y a-humuleno. Conclusiones: los aceites presentaron actividad bactericida frente a los microorganismos testados y los constituyentes en ellos encontrados son compatibles con los encontrados en otros trabajos. Introduction: Guava, Surinam cherry and strawberry guava are native South American fruit trees and the tea from their leaves is traditionally used to treat diarrhea. Objective: to evaluate the antimicrobial action of Psidium guajava L., Eugenia uniflora L. and Psidium cattleyanum Sabine and their constituents. Methods: the essential oils were extracted by a modified Clevenger. The analysis of essential oils was performed on chromatograph (GC/MS). The antimicrobial activity of obtained oils against Escherichia coli, Salmonella typhimurium and Staphylococcus aureus was evaluated by broth microdilution assay to determine their minimum bactericidal concentration. Results: the guava oil showed bactericidal activity against E. coli at 2 % and 8 % concentration rates against S. aureus and S. typhimurium. The strawberry guava oil showed no bactericidal activity against tested microorganisms whereas Surinam cherry had bactericidal activity at 8 % concentration against S. aureus and S. typhimurium. The main constituents commonly found in the three plant oils were a-copaene and a-humulene. Conclusions: the oils showed bactericidal activity against tes
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