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Search Results: 1 - 10 of 29502 matches for " Luis Valledor "
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Proteomic Analysis through Adventitious Rooting of Pinus radiata Stem Cuttings with Different Rooting Capabilities  [PDF]
Carolina álvarez, Luis Valledor, Patricia Sáez, Manuel Sánchez-Olate, Darcy Ríos
American Journal of Plant Sciences (AJPS) , 2016, DOI: 10.4236/ajps.2016.714174
Abstract: In forest production systems, vegetative propagation of elite clones through adventitious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts have been made to identify markers or/and regulators of the aging process and adventitious rooting, molecular mechanisms that regulate both processes are scarcely known, especially at protein level. This research evaluated qualitative and quantitative changes in protein accumulation during the adventitious rooting process of P. radiata stem cuttings, with different rooting capabilities. Beside, an analysis of morpho-anatomical changes was performed in stem cuttings with high and low rooting capabilities, during the adventitious rooting process. It was observed that juvenile 1-year-old stem cuttings rooted in a 100%, while aged stem cuttings (3-year-old) presented only a 20% of rooting. According to the results of differential protein accumulation, univariate and multivariate analysis indicated that in total, 114 and 89 proteins were differentially accumulated in juvenile and aged cuttings, respectively. Also, identification of such proteins showed the presence of proteins related to cell wall organization and the presence of a protein related with proper distribution of auxin PIN transporter, both key in the new meristem formation process during adventitious rooting.
Changes in Gene Expression in Needles and Stems of Pinus radiata Rootstock Plants of Different Ontogenic Age  [PDF]
Carolina Alvarez, Luis Valledor, Patricia Sáez, Rodrigo Hasbún, Manuel Sánchez-Olate, María Jesús Ca?al, Darcy Ríos
American Journal of Plant Sciences (AJPS) , 2016, DOI: 10.4236/ajps.2016.78116
Abstract: A major problem in forest clonal productivity is the loss of morphogenetic capability with the increasing age of plants. However, despite of the importance of loss of morphogenetic competence, very little research has been done about the underlying mechanisms involved in this process. For this reason, a gene expression analysis using dot blot technique was performed in needles and stems of 1- and 3-year old Pinus radiata rootstock plants with a proved decrease in morphogenetic competence. Needles of one year old rootstock plants showed a higher number of up-regulated in genes mainly corresponding to photosynthesis and protein synthesis, degradation and modification, reflecting a higher number of active pathways in younger hedges, contrary to the older ones. Gene expression profiles found in stems are in agreement with those found in needles, indicating more active pathways in younger rootstock plants than in older ones. Several transcripts regulating transcription and translation were up-regulated in young competent tissues. Three-year-old stems presented an increase in the expression of an ethylene response factor, involved in plant organ senescence, indicating that pathways involved in senescence and ageing might inhibit the adventitious root formation, as in the older cuttings.
Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea
Mónica Meijón, Isabel Feito, Luis Valledor, Roberto Rodríguez, María Ca?al
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-10
Abstract: The results of this work indicate that epigenetic mechanisms such as DNA methylation and histone H4 acetylation have opposite and particular dynamics during the transition from vegetative to reproductive development in the apical shoots of azalea. Global levels of DNA methylation and histone H4 acetylation as well as immunodetection of 5-mdC and acetylated H4, in addition to a morphological study have permitted the delimitation of four basic phases in the development of the azalea bud and allowed the identification of a stage of epigenetic reprogramming which showed a sharp decrease of whole DNA methylation similar to that is defined in other developmental processes in plants and in mammals.The epigenetic control and reorganization of chromatin seem to be decisive for coordinating floral development in azalea. DNA methylation and H4 deacetylation act simultaneously and co-ordinately, restructuring the chromatin and regulating the gene expression during soot apical meristem development and floral differentiation.In the ornamental plant industry, azalea production represents a cultivation in expansion. For commercial purposes azalea plants must be compact and well branched [1], although floral quality is the fundamental trait for the commercial success of these species. The flowering promotion, by both increasing the number of flowers and advancing the time of flowering, as well as creating novelty in the flower structure, are major desirable traits in ornamental plant breeding.The annual cycle of azalea japonica in Asturias (Spain, Europe) involves a period of vegetative active growth from May to September, followed by an apparent rest from October to January, months in which well formed buds are visible. As from January, the floral buds are developed, culminating in March with full bloom. Authors such as Bodson [2] defined four phenological stages in the flower development of azalea:1) transition of the apex from the vegetative to floral condition; 2) development of
El factor bronquial en la patología respiratoria del ni o: Las Atelectasias y sus secuelas
Revista chilena de pediatría , 1947,
Análisis clínico de 91 casos de vasculitis de mediano y peque?o vaso en adultos
González Quijada,S.; Sevil Puras,M.a; Valledor,A. R.; Loma,R.;
Anales de Medicina Interna , 2003, DOI: 10.4321/S0212-71992003000900004
Abstract: objective: to evaluate the clinical and biological differences between medium sized vessel vasculitis and small vessel vasculitis. patients and methods: descriptive and retrospective study of 91 patients with vasculitis attended in our hospital from january 1991 to mars 2001. we describe the characteristics of clinical and analytic features. results: 57% were males. the mean age was 61.9±18.6 years (17 to 90 years). the symptoms and affected organs were: palpable purpura (89%), fever (36%), asthenia (20%), arthromyalgias (19%), nephropaty (18%), arthritis (16%), abdominal pain (16%), neuropathy (8,7%), pulmonary involvement (6,5%). 25% had several episodes, lasting clinical, chronic disease. 42% had evidence of two or more involve organs. the patients with pauci-inmune vasculitis presented more asthenia, nephritis, pulmonary involvement, multi-organic involvement and mortality related to the process. we did not found significant differences respect to the others clinical manifestations analysed. conclusions: there is a substantial overlap among different vasculitis, the presence or absence of some clinical and biological features can help in the differentiation and characterization of the different entitles.
Neumonía en el anciano: ?es atípica su presentación clínica?
González Quijada,S.; Galdós Barroso,M.; Riego Valledor,A.; Fernández álvarez,G.; Galindo Vicente,Ma. C.;
Anales de Medicina Interna , 2001, DOI: 10.4321/S0212-71992001000300004
Abstract: objective: some new studies have questioned the often atypical character of pneumonia in aged patients. therefore to study the different clinical presentation of this patology is our aim. material and methods: retrospective analysis of 179 cases of pneumonia in patients aged older than 65 years who were admitted in our hospital during 1992. results: the average age of the patients was 78 years, being 65.9% of them men. 91% presented pneumonias adquired in the community. 73.1% showed a typical dominant clinical presentation (febrile-respiratory); respiratory (47.4%), febrile (25.7%). the rest presented atypical patterns; mental-neurologic or "cerebral dysfunction" (10.6%), perambulation-general state (6.15%) and digestive-abdominal (9.5%). the respiratory pattern was associated to the presence of respiratory antecedents (p<0.001); the febrile pattem to the absence of cardiovascular antecedents (p<0.05); the mental-neurologic to the presence of neurologic antecedents (p<0.001) and the alteration of perambulation-general state to an evolution of more than 7 days (p<0.05). conclusions: the greatest number of our pneumonia patients older than 65 years follows a typical clinical pattern febrile-respiratory). the patient?s prior pathology conditions the clinical presentation.
Maltrato infantil en hijos de madres adolescentes en el Hospital Docente Ginecoobstétrico de Guanabacoa (2005-2006)
Campo González,Ana; Alonso Uría,Rosa María; Amador Morán,Rafael; Díaz Aguilar,Rosa; Durán Valledor,María Isabel; Ballesté López,Irka;
Revista Cubana de Medicina General Integral , 2011,
Abstract: introduction: the infantile ill-treatment syndrome is a phenomenon appearing with the man. it is not present in a isolated way, but involving many biosychosocial factors. objective: to characterize the infantile ill-treatment in children from adolescent mothers. methods: a longitudinal, prospective and descriptive study was conducted in all children from adolescent mothers born in the gynecology and obstetric hospital of guanabacoa during 2005-2006. sample included 62 children in whom it was possible to confirm the ill-treatment and who were followed-up monthly in the consultation until the first year old. as information source authors used the medical records of mothers and the questioning performed in them. results were showed in tables. results: the 82.3 % belonged to mothers aged 15-19, the 50.1 % to unmarried mothers, in a 74.2 % there was family dysfunction. there was ill-treatment backgrounds in mothers for a 60.3 %; the great incidence of ill-treatment to children was the physical negligence for a 62.9 %. conclusions: the physical negligence is noted frequently in the adolescent mothers.
Evaluación de la reproducibilidad de la recogida de datos para el APACHE II, APACHE III adaptado para Espa?a y SAPS II en 9 Unidades de Cuidados Intensivos en Espa?a
Domínguez,L.; Enríquez,P.; álvarez,P.; Frutos,M. de; Sagredo,V.; López-Messa,J.; Carriedo,D.; Taboada,F.; García-Labattut,á.; Gandía,F.; Valledor,M.; Blanco,J.;
Medicina Intensiva , 2008,
Abstract: objective. to assess reproducibility in data collection and its influence on the calculation of the severity scoring and mortality risk in apache ii, apache iii adapted for spain and saps ii. design. multicenter, prospective, observational cohort study. setting. nine spanish intensive care units (icus). patients. 1,211 consecutive patients admitted during the study period were included. those patients under 16 years of age, those with a stay in the icu of less than 24 hours, those admitted for scheduled pacemaker implant and those readmitted to the icu within the same hospital admission were excluded. intervention. none. endpoints of interest. the data needed to calculate the severity and mortality risk scores were collected. a total of 10% of the patients were chosen by simple random sampling and the same data were collected by an independent group of intensive care physicians. finally, the data obtained by the two groups of intensivists were compared. results. significant differences were detected in the acute physiology score (aps) and severity score used for the calculation of apache iii and saps ii, and the predicted risk of death calculated for saps ii. the percentage of agreement on admission diagnosis to the icu was 50% for both apache ii and iii models. nonetheless, in most of the patients (76.58% for apache ii and 79.82% for apache iii), the difference in the predicted risk of death due to the different assignation of diagnoses on admission to the icu was less than 10%. conclusions. in this study, aps was the most influential factor on the reproducibility of severity scores and risk of death prediction. admission diagnosis assignment had no significant impact on the reproducibility of the predicted mortality risk.
Mortalidad y estancia hospitalaria ajustada por gravedad como indicadores de efectividad y eficiencia de la atención de pacientes en Unidades de Cuidados Intensivos
Domínguez,L.; Enríquez,P.; álvarez,P.; Frutos,M. de; Sagredo,V.; Domínguez,A.; Collado,J.; Taboada,F.; García-Labattut,á.; Bobillo,F.; Valledor,M.; Blanco,J.;
Medicina Intensiva , 2008,
Abstract: objective. to evaluate effectiveness and efficiency of icu care using the apache iii model customized for spain. desing. prospective, observational, multi-centre cohort study. setting. nine spanish icus. patients. one thousand two hundred and eleven patients admitted consecutively. patients under 16 years of age, patients admitted for less than 24 hours, patients admitted for definitive pacemaker implant and icu readmissions within the same hospital stay were excluded. primary endpoints. all the necessary endpoints to calculate the apache iii score and predicted mortality risk were collected. the standardized mortality ratio (smr) was calculated. the actual weighted hospital days (awhd) was calculated according to the patient's status and patient's location (ward or icu). the weighted hospital days predicted (whdp) was calculated according to the multiple regression model that provided the best explanation of the hospital stay. later, the standardized resource use performance index (srupi), computed as awhd/whdp, was calculated for each hospital. results. the smr was 0.9 (95% ci: 0.82-0.99), srupi was greater than 1 in 3 of 9 hospitals, according the smr and srupi only one hospital was qualified as an outlier. conclusions. in this study, no relationship was found between quality of care and use of resources. moreover, this methodology may be a useful tool in order to detect deviations from the standard of care and use of resources, and in this way to lead to the analysis of different causes, the differences among hospitals being taken into account.
Introducing Engineering Students to Historical/Cultural Perspectives through Story-Centered On-Line Learning  [PDF]
Luis A Godoy
Creative Education (CE) , 2011, DOI: 10.4236/ce.2011.22009
Abstract: Several authors argued that history of science should be an integral part of science education; however, there are many obstacles to carry out an implementation within this approach, including that lecturers normally lack a necessary historical background (a situation that is largely aggravated in engineering faculties), and the impossi- bility of including new courses or credits in an already tight curriculum. The development of on-line modules that engineering students can work outside their normal schedule of classes, introducing historical/cultural per- spectives, is presented in this paper. E-learning and knowledge management strategies are used in the context of science education at undergraduate and graduate levels. The approach is based on learning-by-doing in a virtual environment, and specifically presents story-centered activities, in which the student is faced with a problem and plays the role of an expert to provide a solution to the case. The specific case considered develops in the form of a controversy concerning the origin of the studies of column buckling. Two sets of information are available for navigation in the module: specific information that the student can explore (containing historical material di- rectly connected to the case provided), and more general information (providing the historical/cultural context to the problem). A first application has been made with civil engineering students, who had to write a two-page white paper as a consequence of their work on the problem. The interest generated in the participating students and the positive evaluation of their experience seems to indicate that this type of activity can serve to enhance traditional engineering lectures by incorporating a historical dimension. The present web-based approach could be extended to tackle similar conflicts in fields for which there is ample documentation available in the literature or in other historical episodes which may lead to rich discussions.
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