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Search Results: 1 - 10 of 5323 matches for " Luciana Migliore "
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Assessment of Posidonia Oceanica (L.) Delile conservation status by standard and putative approaches: the case study of Santa Marinella meadow (Italy, W Mediterranean)  [PDF]
Alice Rotini, Carla Micheli, Luigi Valiante, Luciana Migliore
Open Journal of Ecology (OJE) , 2011, DOI: 10.4236/oje.2011.12006
Abstract: The conservation status of the Posidonia oceanica meadow at Santa Marinella (Rome) was evaluated through both standard (bed density, leaf biometry, \"A\" coefficient, Leaf Area Index, rhizome production) and biochemical/genetic approaches (total phenol content and Random Amplified Polymorphic DNA marker). The bio-chemical/genetic results are in agreement with those obtained by standard approaches. The bed under study was ranked as a disturbed one, due to its low density, and high heterogeneity in leaf biometry, LAI values, \"A\" coefficient and primary production. This low quality ranking is confirmed by both mean phenol content in plants, quite high and scattered, and by the low genetic variability in the meadow, with a very high similarity of specimen at a local scale. Hence, these two putative approaches clearly identify the endangered conservation status of the meadow. They link plant biodiversity and ecophysiology to ecosystem 'health'. Furthermore, they are repeatable and standardizable and could be usefully introduced in meadows monitoring to check environmental quality.
Comparative analysis of bed density, total phenol content and protein expression pattern in Posidonia oceanica (L.) Delile  [PDF]
Alice Rotini, Letizia Anello, Maria Di Bernardo, Agata Giallongo, Luigi Valiante, Luciana Migliore
Open Journal of Ecology (OJE) , 2013, DOI: 10.4236/oje.2013.36050

Posidonia oceanicameadows are experiencing a progressive decline, and monitoring their status is crucial for the maintenance of theseecosystems. We performed a comparativeanalysis of bed density, total phenol content and protein expression pattern to assess the conservation status ofPosidoniaplants from the S. Marinella (Rome, Italy) meadow. The total phenol content was inversely related to maximum beddensity, confirming the relationship betweenhigh phenol content and stressful conditions. In addition, protein expression pattern profilesshowed that the number of differentially expressed proteins was dramatically reduced in the latest years compared to previous analyses. Our results support the usefulness of integrating solid descriptors, such as phenol content, with novel biochemical/molecular approaches in the monitoring of meadows.

The Relationship between Salinity and Bacterioplankton in Three Relic Coastal Ponds (Macchiatonda Wetland, Italy)  [PDF]
Matteo Evangelisti, Domenico D’Amelia, Gustavo Di Lallo, Maria Cristina Thaller, Luciana Migliore
Journal of Water Resource and Protection (JWARP) , 2013, DOI: 10.4236/jwarp.2013.59087

The great environmental importance of wetlands is linked to the high biodiversity of flora and fauna they support, so that the international Ramsar Convention focused on these areas and highlighted the need to preserve them. The bacterial communities that thrive in these ecosystems play a key role in regulating the local biogeochemical processes and yet their distribution, abundance and dynamics are poorly known. This work is aimed to study the bacterial assemblages over a year long, to contribute to the understanding of the natural processes occurring in wetlands at variable salinity. The knowledge of bacterial groups, species or assemblages can provide a useful bioindicator for conservation and restoration efforts. Macchiatonda Natural Reserve (Santa Severa, Rome, Italy) is a relic ecosystem, once found along the entire Tyrrhenian coast. This wetland encompasses three coastal ponds with different salinity, where both peculiar vegetation and highly diverse migratory and resident avifauna can be found. This ancient system has been scarcely investigated and nothing is known about its microbial community. The molecular metagenomic analyses performed to investigate the salinity/bacterioplankton relationship, highlighted differences in the bacterial structure, between ponds and seasons. Analogous trends in SSCP profiles, Shannon Index, and bacterial composition (16S) were observed in the two saltier ponds, whereas the entire set of results was different for the less salty one. The species diversity in the three ponds varied according the salinity gradient, with the maximum diversity corresponding to a salt concentration range between 20 and 30. At higher and lower salinity, the microbial diversity lowers, according to the Intermediate Disturbance Hypothesis.

Phenols content and 2-D electrophoresis protein pattern: a promising tool to monitor Posidonia meadows health state
Luciana Migliore, Alice Rotini, Davide Randazzo, Nadia N Albanese, Agata Giallongo
BMC Ecology , 2007, DOI: 10.1186/1472-6785-7-6
Abstract: The overall expression pattern from Posidonia rhizome was determined using a preliminary proteomic approach, 437 protein spots were characterized by pI and molecular weight. We found that protein expression differs in samples belonging to sites with high or low phenols: 22 unique protein spots are peculiar of "low phenols" and 27 other spots characterize "high phenols" samples.Posidonia showed phenols variations within the meadow, that probably reflect the heterogeneity of environmental pressures. In addition, comparison of the 2-D electrophoresis patterns allowed to highlight qualitative protein expression differences in response to these pressures. These differences may account for changes in metabolic/physiological pathways as adaptation to stress. A combined approach, based on phenols content determination and 2-D electrophoresis protein pattern, seems a promising tool to monitor Posidonia meadows health state.Posidonia oceanica (L.) Delile (fig. 1) is a Mediterranean endemism. Plants colonize soft bottoms producing large meadows that span from the sea surface to 35–40 m depth. Meadows are highly productive ecosystems, as they produce high amount of oxygen and organic compounds, sustain complex food nets, act as a nursery/refuge for several species. They also play a crucial role in coastal preservation, by stabilizing sediments and reducing hydrodynamics effects (see fig. 1) [1,2].Many human activities and natural events are responsible for the widespread meadows regression, such as modified hydrogeological regime and littoral transport [3-6], pollution [7-11], aquaculture [12-15], trawling [16,17], anchorages [18-21], placing of cable/pipes or damping [22,23], in addition to grazing, sea storms, climatic changes, etc. [24-26]. All lead to alterations of Posidonia ecosystems.Phenolic compounds are widespread secondary metabolites in plants. They play a role in herbivore/pathogen protection [27,28] and are considered stress indicators in terrestrial plants [29-38
Know Your Current Ih: Interaction with a Shunting Current Explains the Puzzling Effects of Its Pharmacological or Pathological Modulations
Michele Migliore, Rosanna Migliore
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0036867
Abstract: The non-specific, hyperpolarization activated, Ih current is particularly involved in epilepsy and it exhibits an excitatory or inhibitory action on synaptic integration in an apparently inconsistent way. It has been suggested that most of the inconsistencies could be reconciled invoking an indirect interaction with the M-type K+ current, another current involved in epilepsy. However, here we show that the original experiments, and the simplified model used to explain and support them, cannot explain in a conclusive way the puzzling Ih actions observed in different experimental preparations. Using a realistic model, we show instead how and why a shunting current, such as that carried by TASK-like channels, and dependent on Ih channel is able to explain virtually all experimental findings on Ih up- or down-regulation by modulators or pathological conditions. The model results suggest several experimentally testable predictions to characterize in more details this elusive and peculiar interaction, which may be of fundamental importance in the development of new treatments for all those pathological and cognitive dysfunctions caused, mediated, or affected by Ih.
Tracking Acquired Antibiotic Resistance in Commensal Bacteria of Galápagos Land Iguanas: No Man, No Resistance
Maria Cristina Thaller,Luciana Migliore,Cruz Marquez,Washington Tapia,Virna Cede?o,Gian Maria Rossolini,Gabriele Gentile
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0008989
Abstract: Antibiotic resistance, evolving and spreading among bacterial pathogens, poses a serious threat to human health. Antibiotic use for clinical, veterinary and agricultural practices provides the major selective pressure for emergence and persistence of acquired resistance determinants. However, resistance has also been found in the absence of antibiotic exposure, such as in bacteria from wildlife, raising a question about the mechanisms of emergence and persistence of resistant strains under similar conditions, and the implications for resistance control strategies. Since previous studies yielded some contrasting results, possibly due to differences in the ecological landscapes of the studied wildlife, we further investigated this issue in wildlife from a remote setting of the Galapagos archipelago.
Il senso della fine. Arsure, Urbs Incensa The sense of the end. Arsura, Urbs Incensa
Tiziana Migliore
Galáxia , 2012,
Abstract: Lidea della fine e quella del senso sono legate da un rapporto complesso e problematico. Lo si evince dal termine latino finis, che ha una polisemia assente in altre lingue indoeuropee. Infatti finis vuol dire confine, limite, compimento, ma anche intento, mira, meta. Tale polisemia si conserva in italiano e anzi si divarica nelle due diverse accezioni di punto ultimo, cessazione, rovina e risultato, riuscita, scopo. La lingua italiana dispone anche dellaggettivo finis, che ha diversi usi e applicazioni nelletica e nellestetica. Questo saggio esplora il campo semantico-concettuale del(la) fine da una prospettiva semiotica, indagando limmaginario occidentale della citt in fiamme nella storia dellarte e nella letteratura artistica. Attraverso il caso studio dellincendio, ci si interroga sulla visibilit dellazione del fuoco (ardore) e sui suoi effetti (arsura), dal divampare delle fiamme ai residui in forma di cenere. There is a complex and controversial relationship between the ideas of the end and of sense. This is evident in the Latin word finis, which presents numerous semantic meanings lacking in other Indo-European languages. In fact, finis means border, limit, end, achievement, goal, finality, purpose, just to mention a few. This polysemanticity is kept in Italian and is shaped around two crucial meanings. On one hand, it means end and ruin, whereas, on the other, implies result, achievement, aim. Furthermore the Italian word has been enriched by the adjective fine, which has acquired several meanings even in Ethics and Aesthetics. This contribution explores this conceptual field from a semiotics perspective, analyzing the Western imagery of the burning city in art history and in the artistic literature. The essay points out the visibility of fire action and its effects, from the flaring up of flames to the residual ash.
Una disciplina de la calidad: Perfil de Omar Calabrese
Tiziana Migliore
Tópicos del seminario , 2012,
Evaluación de la estenosis aórtica mediante la relación gradiente pico/gradiente medio:
Ricardo Migliore
Revista Argentina de Cardiología , 2006,
Discovery or invention? The difference between art and communication according to Ernst Gombrich
Tiziana Migliore
Journal of Art Historiography , 2011,
Abstract: This paper sets out to explore the boundaries between artworks and advertising works in line with the thinking of Ernst Gombrich. His distinction between art and other forms of communication emphasises the importance of ‘discovery’ as opposed to mere ‘invention’. What are the symptoms of an artwork according to Gombrich? The difference with advertising is not ontological at all: anything that comes out of the artist’s top hat and is traded between dealers, gallery owners and collectors is art, whereas any graphic work produced for communication purposes and conveyed by the media is propaganda. Ultimately the most valid distinction lies in the modality and the internal dynamics of the artwork itself. By re-examining some examples provided by Gombrich – from the colour of light in John Constable to Giulio Romano’s Palazzo Te, from Raphael’s Madonna della sedia to Saul Steinberg’s work – this paper delves further into an issue not comprehensively dealt with by Gombrich and whose importance is still underestimated.
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