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Search Results: 1 - 10 of 7539 matches for " Luciana CS;Ribas "
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Schistosomal myeloradiculopathy due to Schistosoma mansoni: report on 23 cases
Nobre Vandack,Silva Luciana CS,Ribas Jo?o G,Rayes Abdunnabi
Memórias do Instituto Oswaldo Cruz , 2001,
Abstract: Schistosoma mansoni infection is likely to be responsible for a significant proportion of cases of myelopathy occurring in areas where schistosomiasis is endemic. The aim of this study is to describe the clinical, laboratory and therapeutic data of 23 patients with schistosomal myeloradiculopathy. The medical records of 23 patients with schistosomal myelopathy admitted to two general hospitals of Belo Horizonte (MG), in Brazil, from 1995 to 1999, were reviewed retrospectively. Seventeen patients were male (74%). The mean age for the whole group was 27 years. Lower limb weakness and associated lumbar and/or lower limb pain were reported by 20 patients (87%), and 16 (70%) were unable to walk. All individuals presented urinary retention and 19 (83%) complained of intestinal dysfunction. The treatment was based on the association of antischistosomal drugs and corticosteroids. Five patients (22%) presented a full response to treatment, 13 (57%) partial response without functional limitations and 4 (17%) partial improvement with limitations or no response. Three out of the 4 patients who stopped steroids before 45 days of treatment developed recurrence of the symptoms and signs of myelopathy. Our cases demonstrate the severe presentation of the disease and the data disclosed here suggest that treatment with steroids should be kept for months after clinical improvement.
Schistosomal myeloradiculopathy due to Schistosoma mansoni: report on 23 cases
Nobre, Vandack;Silva, Luciana CS;Ribas, Jo?o G;Rayes, Abdunnabi;Serufo, JC;Lana-Peixoto, MA;Marinho, Rosana FZ;Lambertucci, JR;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000900020
Abstract: schistosoma mansoni infection is likely to be responsible for a significant proportion of cases of myelopathy occurring in areas where schistosomiasis is endemic. the aim of this study is to describe the clinical, laboratory and therapeutic data of 23 patients with schistosomal myeloradiculopathy. the medical records of 23 patients with schistosomal myelopathy admitted to two general hospitals of belo horizonte (mg), in brazil, from 1995 to 1999, were reviewed retrospectively. seventeen patients were male (74%). the mean age for the whole group was 27 years. lower limb weakness and associated lumbar and/or lower limb pain were reported by 20 patients (87%), and 16 (70%) were unable to walk. all individuals presented urinary retention and 19 (83%) complained of intestinal dysfunction. the treatment was based on the association of antischistosomal drugs and corticosteroids. five patients (22%) presented a full response to treatment, 13 (57%) partial response without functional limitations and 4 (17%) partial improvement with limitations or no response. three out of the 4 patients who stopped steroids before 45 days of treatment developed recurrence of the symptoms and signs of myelopathy. our cases demonstrate the severe presentation of the disease and the data disclosed here suggest that treatment with steroids should be kept for months after clinical improvement.
Efeito da idade da muda e da esta??o do ano no enraizamento de miniestacas de Pinus taeda L.
Alcantara, Giovana Bomfim de;Ribas, Luciana Lopes Fortes;Higa, Ant?nio Rioyei;Ribas, Katia Christina Zuffellato;Koehler, Henrique Soares;
Revista árvore , 2007, DOI: 10.1590/S0100-67622007000300005
Abstract: pinus taeda l. is the main forest tree species planted in southern brazil and its wood is used for sawlogs, venner, particle board, medium density fiberboard (mdf), pulp and paper industries. due to its high economic importance, interest exists in developing techniques for vegetative propagation aiming at mass multiplication. the purpose of the present work was to evaluate the effect of the age of seedlings used as mother plants (60, 90, 120 and 150 day-old) and of the four seasons of the year on rooting of p. taeda minicuttings. minicuttings of 5 cm in length were collected from herbaceous branches and treated with captan? solution (0.1%). minicuttings were planted in plastic tubes containing mecplant? substrate overlayered with vermiculite. minicuttings were maintained in greenhouse for 120 days with temperature between 15 and 25oc and 90% relative humidity. the parameters evaluated were: percentage of rooted, surviving and dead minicuttings, length of the three larger roots, number of roots and minicutting dry weight. seedling age influenced the rooting percentage, and the highest value (85%) was obtained with 60-day-old seedlings. the winter was the most favorable season for the collection of minicuttings.
Activation of c-Jun N-Terminal Kinase (JNK) during Mitosis in Retinal Progenitor Cells
Vinicius Toledo Ribas, Bruno Souza Gon?alves, Rafael Linden, Luciana Barreto Chiarini
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034483
Abstract: Most studies of c-Jun N-terminal Kinase (JNK) activation in retinal tissue were done in the context of neurodegeneration. In this study, we investigated the behavior of JNK during mitosis of progenitor cells in the retina of newborn rats. Retinal explants from newborn rats were kept in vitro for 3 hours and under distinct treatments. Sections of retinal explants or freshly fixed retinal tissue were used to detect JNK phosphorylation by immunohistochemistry, and were examined through both fluorescence and confocal microscopy. Mitotic cells were identified by chromatin morphology, histone-H3 phosphorylation, and location in the retinal tissue. The subcellular localization of proteins was analyzed by double staining with both a DNA marker and an antibody to each protein. Phosphorylation of JNK was also examined by western blot. The results showed that in the retina of newborn rats (P1), JNK is phosphorylated during mitosis of progenitor cells, mainly during the early stages of mitosis. JNK1 and/or JNK2 were preferentially phosphorylated in mitotic cells. Inhibition of JNK induced cell cycle arrest, specifically in mitosis. Treatment with the JNK inhibitor decreased the number of cells in anaphase, but did not alter the number of cells in either prophase/prometaphase or metaphase. Moreover, cells with aberrant chromatin morphology were found after treatment with the JNK inhibitor. The data show, for the first time, that JNK is activated in mitotic progenitor cells of developing retinal tissue, suggesting a new role of JNK in the control of progenitor cell proliferation in the retina.
Anatomia comparada das folhas e raízes de Cymbidium Hort. (Orchidaceae) cultivadas ex vitro e in vitro
Mayer, Juliana Lischka Sampaio;Ribas, Luciana Lopes Fortes;Bona, Cleusa;Quoirin, Marguerite;
Acta Botanica Brasilica , 2008, DOI: 10.1590/S0102-33062008000200003
Abstract: during in vitro culture plants are kept in an atmosphere with high relative humidity, low light intensity and reduced gas exchange, resulting in low transpiration rates. therefore, when these plants are exposed to ex vitro conditions, they suffer stress, which can induce mortality. the purpose of this study was to compare the anatomical structure of cymbidium 'joy polis' plants from ex vitro (mother plant and acclimatized plants) and in vitro cultures and to verify if the anatomical structure of in vitro cultured plants affects acclimatization. the ex vitro plants were kept in a greenhouse in pots containing a mixture of coconut-fiber powder and coconut fiber. the in vitro plants were kept in ms culture medium. for the qualitative anatomical analysis, samples of leaves and roots were collected from ex vitro and in vitro plants. the acclimatized plants presented morphological and anatomical structure similar to the mother plant. the anatomical structure of in vitro plants did not affect plant survival during the acclimatization process, as this cultivar has great phenotypic plasticity. cymbidium 'joy polis' plants have high capacity of adaptation to the environment and this characteristic is likely to be responsible for their survival rate of 100% during acclimatization.
Multiplica??o in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia)
Machado, Marília Pereira;Biasi, Luiz Antonio;Ritter, Marlice;Ribas, Luciana Lopes Fortes;Koehler, Henrique Soares;
Ciência e Agrotecnologia , 2006, DOI: 10.1590/S1413-70542006000400009
Abstract: the micropropagation is one technique that makes possible the massal propagation of plants of economic interest. the objective of this work was to evaluate the effect of 6-benzilaminopurina (bap) and kinetin (kin) in the multiplication in vitro of the grapevine rootstock 'vr043-43'. the isolation was carried through in culture medium ql, supplemented with bap or kin, in the concentrations of 0; 2,5; 5,0 and 10,0μm. the subcultures had been carried out each 45 days. the number of shoots per explant increased along with the subcultures with bap. however this effect was not observed with the kin that it got resulted similar to the one of the cytokin-free (1,0 shoot/explant). the concentrations of 5,0 nd 10,0 μm of bap had promoted the biggest number of shoots with reduction of the height. the highest concentration of kin (10,0 μm) also had negative effect in the height of the shoots, as well as, in the number of leaves per shoot. the callus formation (100%) was observed with bap and kin in all the concentrations, and in the cytokinin absence it did not have the callus formation. cytokinin bap reduced the formation of roots that was of 100% with the kin and cytokinin-free.
Acclimatization of 'VR043-43' (Vitis vinifera x Vitis rotundifolia) grapevine rootstock
Machado, Marília Pereira;Biasi, Luiz Antonio;Ritter, Marlice;Ribas, Luciana Lopes Fortes;Zanette, Flávio;
Scientia Agricola , 2010, DOI: 10.1590/S0103-90162010000400007
Abstract: the pre-acclimatization stage can be used to improve micropropagation protocols and increase the yield of produced plants. the influence of sucrose and photon flux density (pfd) levels on the acclimatization of in vitro-grown 'vr043-43' (vitis vinifera x vitis rotundifolia) grapevine rootstocks was evaluated. rooted shoots were obtained from 4-week-old in vitro shoots cultivated in ql (quoirin and lepoivre, 1977) culture medium supplemented with 15, 30 and 45 g l-1 of sucrose. the experiment was kept in a 25 ± 2oc growth room, under 16-h photoperiod and pfd of 18 μmol m-2 s-1 or 43 μmol m-2 s-1. plants were transferred to an intermittent misting system greenhouse for 10 d followed by 20 d of once-a-day watering routine using a handheld hose. plant height was influenced by sucrose concentration, and shoots produced on media supplemented with 30 g l-1 sucrose were the tallest (5.0 cm). the largest leaf area was obtained with 31.3 g l-1 of sucrose, under the pfd of 43 μmol m-2 s-1 (13.3 cm2). absence of sucrose in the culture medium led to a significant reduction in leaf area at both pfds. shoot (aerial part) dry matter was largest when 30 or 45 g l-1 of sucrose (17.5 and 16.7 mg per plant, respectively) were used. microcuttings rooted in all sucrose concentrations tested. the highest survival percentage (100%) during ex vitro acclimatization was obtained for shoots cultured in media supplemented with 45 g l-1 of sucrose under both pfds tested.
Micropropaga??o de Aspidosperma polyneuron (peroba-rosa) a partir de segmentos nodais de mudas juvenis
Ribas, Luciana Lopes Fortes;Zanette, Flávio;Kulchetscki, Luiz;Guerra, Miguel Pedro;
Revista árvore , 2005, DOI: 10.1590/S0100-67622005000400003
Abstract: the objective of the present work was to establish a micropropagation protocol of aspidosperma polyneuron from juvenile material. apical shoots from two years old seedlings were collected in a greenhouse and sterilised with naocl or hgcl2 to establish aseptic cultures. multiple shoots induction was evaluated in wpm medium, supplemented with bap, zea or kin (2.2 - 8.8 mm) in initial culture and two subsequent subcultures. the elongation of shoots was tested with growth regulators combinations: 2.25 mm of bap, zea or kin with 1.25 mm of iba. iba treatments (2.5; 5.0 and 10 mm) were tested with 5 and 15 minutes to induce roots. plantlets were planted in a greenhouse. efficient apical shoots sterilization was achieved with naocl (0,25% - 10 minutes) or hgcl2 (0.05%-10 minutes); survival rates were 72.89% and 84,10%, respectively. apical shoots induced 4-5 axillary buds in wpm culture medium, containing zea or bap (4.4 - 8.8 mm) following two subcultures. reduced concentrations of zea or bap (2.25 mm), combined with iba (1.25) produced elongated shoots. iba treatment (10 mm) during 15 minutes induced higher rooting percentages (80%). plantlets planted in a greenhouse showed higher survival rates (90%).
Micropropaga??o de Cabralea canjerana
Rocha, Silvana Cruz da;Quorim, Marguerite;Ribas, Luciana Lopes Fortes;Koehler, Henrique Soares;
Revista árvore , 2007, DOI: 10.1590/S0100-67622007000100006
Abstract: cabralea canjerana (vell.) mart. (meliaceae) ("canjarana") is a native tree of economic importance in brazil. the storage of seeds is of short duration and it is therefore necessary to establish a protocol for micropropagation of this species. in this work, multiplication experiments were carried out using nodal segments, excised from in vitro germinated plants. the segments were inoculated in ms or wpm culture medium, supplemented with 6-benzylaminopurine (bap) and/or 2-isopentenyladenine (2-ip) at 2.5 or 5 μm. micro-cuttings were taken from new shoots developed from the seeds and used in a rooting experiment using a culture medium with half-strength ms medium (ms/2) supplemented with indolbutyric acid (iba) (0, 2.5 and 5 μm). after 7 days in this medium, they were transferred to ms/2 medium without auxin under light. during the multiplication phase, the ms culture medium was more suitable for the multiplication of c. canjerana than wpm medium. the nodal segments cultured in the presence of 2.5 μm bap showed the best result, with a multiplication rate of 1.77 per month on ms medium. the rooting of the microcuttings was 87.5% when they were kept in the presence of 5 μm iba for 7 days. an acclimatization rate of 90% was achieved after 30 days in the greenhouse. in conclusion, the micropropagation of c. canjerana from nodal segments of plantlets is possible for this species.
Cutting of the Berberis laurina Billb. Using Different Concentrations of Indolebutyric Acid Estaquia de Berberis laurina Billb. utilizando diferentes concentra es de ácido indol butírico
Michelle Melissa Althaus,Luciana Christina Leal,Fernanda Christina Silveira,Katia Christina Zuffellato-Ribas
Pesquisa Florestal Brasileira , 2011, DOI: 10.4336/2012.pfb.50.99
Abstract: Berberis laurina Billb. (Berberidaceae) is a native shrub from Floresta Ombrófila Mista ecological formation, very ornamental and useful specie for recovery of degraded areas. With medical applications, its fruit is edible and its roots are used as dye. This paper aims to analyze lhe induction of lhe rooting of Berberis laurina stem cuttings employing indolebutyric acid (IBA) in O, 1000 and 2000 mgL-1 concentrations, using the vermiculite as growing medium. In october 2003, stem cuttings were collected from eight stock plants, located in Fazenda Rio Grande - PR. Semi-hardwood cuttings were preppared with 8.0 cm long by 0.35 cm of diameter shape, containing three leaves on the top, cut in a half, the base was cut in diagonal and the top was cut straight, receiving a treatment with sodium hipochlorite (0.5%) for 10 minutes. The experiment was being conducted in a greenhouse. After 90 days an analysis was taken from the rooting rate, rate of the number of roots formed per cuttings, length of the three biggest roots formed per cuttings, rate of the initial leaves which have survived since the cutting’s manufacture per cutting, rate of sprout presence per cutting, rate of not rooted living cuttings and rate of dead cuttings. The analyzed variables did not show a significative difference using 5% of probability, except for the rate of not rooted living cuttings which had differed statistically; however, the rooting rate was above the ones found in studies made with another species of Berberis (20.0 - 30.0 %). IBA did not improve the rooting of B. laurina. Berberis Iaurina (Berberidaceae) é um arbusto nativo da Floresta Ombrófila Mista, de grande potencial ornamental, também utilizado em recupera o de áreas degradadas. Possui aplica o medicinal, seu fruto é comestível e suas raízes s o utilizadas como corantes. Este trabalho teve como objetivo analisar a indu o do enraizamento de estacas caulinares de B. Iaurina pela aplica o de ácido indol butírico (AIB), nas concentra es O, 1000 e 2000 mgL-1, utilizando o substrato vermiculita de granulometria média. Em outubro de 2003, foram coletadas estacas caulinares semilenhosas, de oito plantas matrizes com aproximadamente 10 anos de idade, localizadas no município de Fazenda Rio Grande - PR, confeccionadas com aproximadamente 8,0 cm de comprimento e 0,35 cm de diametro, com 3 folhas na por o apical cortadas pela metade, base cortada em bisel e ápice em corte reto, recebendo tratamento fitossanitário com hipoclorito de sódio a 0,5% por 1O minutos e transferidas para casa-devegeta o climatizada. Após
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