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Search Results: 1 - 10 of 4454 matches for " Lucia Ciccocioppo "
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The tumor suppressor gene KCTD11REN is regulated by Sp1 and methylation and its expression is reduced in tumors
M Michela Mancarelli, Francesca Zazzeroni, Lucia Ciccocioppo, Daria Capece, Agnese Po, Simona Murgo, Raffaello Di Camillo, Christian Rinaldi, Elisabetta Ferretti, Alberto Gulino, Edoardo Alesse
Molecular Cancer , 2010, DOI: 10.1186/1476-4598-9-172
Abstract: TSGs often locate at chromosomal regions, which are frequently deleted and/or methylated in tumors. High levels of 17p13 somatic alterations have been showed in several tumors, distal and independent of the p53 locus [1-4].Our group has identified KCTD11 as an immediate-early gene induced by neurogenic signals [5] and encoding a novel adaptor of Cullin3 ubiquitin E3 ligase complex targeting Histone Deacetylase 1 [6]. Importantly, KCTD11 is a novel TSG that inhibits cell growth and is mapping on human chromosome 17p13.2, whose expression is frequently lost in human MB [4].To analyze whether the down-regulation of KCTD11 represents a specific feature of MB, as well to other cancers, we performed a wide screening for KCTD11 expression, analyzing 177 human tumor samples and 177 normal matching samples, representing 18 different cancer types. Normal tissues, including larynx, esophagus, stomach, colon-rectum, urinary bladder, lung, breast, gallbladder and endometrium, exhibited a nuclear KCTD11 positive immunohistochemical staining between 40 to 78% (Fig. 1B), whereas the matching tumor samples showed a significant reduction of 0 to 18% of nuclear KCTD11 staining (Fig. 1A and 1B). Reduced KCTD11 expression was not observed in thyroid and kidney tumor tissues vs normal suggesting a tumorigenic specific role of KCTD11 for the above mentioned tissues (Fig. 1A and 1B and data not shown). Moreover KCTD11 was undetected both in normal and cancer tissues from liver, lymph-node and exocrine pancreas (data not shown). Together, these findings clearly indicated that selective tissues expressing KCTD11 have down-regulated this gene during tumorigenesis.To understand the transcriptional regulation of KCTD11, we identified and analyzed the promoter region. Human KCTD11 proximal promoter is a 623 bp region (Fig. 2A). It turned out to be a TATA- and CAAT-less promoter. The transcription start site (TSS) was previously identified [4] (Fig. 2A). Using the TRANSFACT software, we identifie
Prognostic Value of HPV E6/E7 mRNA Assay in Women with Negative Colposcopy or CIN1 Histology Result: A Follow-Up Study
Paolo Giorgi Rossi, Maria Benevolo, Amina Vocaturo, Donatella Caraceni, Lucia Ciccocioppo, Antonio Frega, Irene Terrenato, Roberta Zappacosta, Deborah French, Sandra Rosini
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0057600
Abstract: Pap test, and especially HPV DNA test, identify a large group of women who do not have any clinically relevant lesions, i.e., CIN2+ (Cervical Intraepithelial Neoplasia grade 2 or worse), but who are at greater risk of getting lesions in the future. The follow up of these women needs new biomarkers with prognostic value. The objective of this study is to evaluate the prognostic value of E6/E7 mRNA over-expression assay (PreTect HPV-Proofer, Norchip) for 5 HR-HPV types (16, 18, 31, 33, and 45) for progression to CIN2+ after a negative colposcopy. This prospective study, conducted at four Italian centres, enrolled 673 women with either a negative colposcopy or a negative or CIN1 histology. The clinical end-point was histological confirmation of CIN2+. Women were classified at baseline according to mRNA results and managed according to local colposcopy protocols. At least one conclusive follow-up test was obtained for 347 women (25 months average lapse since recruitment, range 5–74). Only seven CIN2+ were detected during follow up, three among the 82 women positive for mRNA at baseline, two among the 250 negative (Fisher exact test, p = 0.02), and two among the 12 with an invalid test. Absolute CIN2+ risk was 6.7/1,000 person/years in the whole cohort. The absolute CIN2+ risk was 18.4/1,000 person/years and 3.6/1,000 person/years in mRNA-positive and mRNA-negative women, respectively. In conclusion, E6/E7 mRNA over-expression appears to be a good candidate as a prognostic biomarker to manage HR-HPV DNA-positive women with negative colposcopy or histology, particularly in order to decrease follow-up intensity in those who are negative.
Therapeutic application of stem cells in gastroenterology: An up-date
Patrizia Burra,Debora Bizzaro,Rachele Ciccocioppo,Fabio Marra
World Journal of Gastroenterology , 2011, DOI: 10.3748/wjg.v17.i34.3870
Abstract: Adult stem cells represent the self-renewing progenitors of numerous body tissues, and they are currently classified according to their origin and differentiation ability. In recent years, the research on stem cells has expanded enormously and holds therapeutic promises for many patients suffering from currently disabling diseases. This paper focuses on the possible use of stem cells in the two main clinical settings in gastroenterology, i.e., hepatic and intestinal diseases, which have a strong impact on public health worldwide. Despite encouraging results obtained in both regenerative medicine and immune-mediated conditions, further studies are needed to fully understand the biology of stem cells and carefully assess their putative oncogenic properties. Moreover, the research on stem cells arouses fervent ethical, social and political debate. The Italian Society of Gastroenterology sponsored a workshop on stem cells held in Verona during the XVI Congress of the Federation of Italian Societies of Digestive Diseases (March 6-9, 2010). Here, we report on the issues discussed, including liver and intestinal diseases that may benefit from stem cell therapy, the biology of hepatic and intestinal tissue repair, and stem cell usage in clinical trials.
BRICS, a Multi-Centre “Legal Network”?  [PDF]
Lucia Scaffardi
Beijing Law Review (BLR) , 2014, DOI: 10.4236/blr.2014.52013
Abstract:

The paper analyzes the phenomenon of the BRICS group and its transformation into BRICs. From the first Summit, whose final declarations were mainly centered on economic, financial and commercial themes, the group attention has broadened its horizons, as to encompass health, agriculture, environment, international relations. The BRICS working method seems to suggest a new pattern of inter-state relations, based on peer-to-peer cooperation, experiences sharing and “soft” policy transfer. Given the difficult classification of this new “entity”, the author suggests considering it more as a network, where there is not a singular hegemony power, but where the relevance of the different countries varies according to the issue discussed in the Summit.

MT-7716, a novel selective nonpeptidergic NOP receptor agonist, effectively blocks ethanol-induced increase in GABAergic transmission in the rat central amygdala
Marsida Kallupi,Christopher S. Oleata,Koji Teshima,Roberto Ciccocioppo,Marisa Roberto
Frontiers in Integrative Neuroscience , 2014, DOI: 10.3389/fnint.2014.00018
Abstract: The GABAergic system in the central amygdala (CeA) plays a major role in ethanol dependence and the anxiogenic-like response to ethanol withdrawal. A large body of evidence shows that Nociceptin/Orphanin FQ (N/OFQ) regulates ethanol intake and anxiety-like behavior. In the rat, ethanol significantly augments CeA GABA release, whereas N/OFQ diminishes it. Using electrophysiological techniques in an in vitro slice preparation, in this study we investigated the effects of a nonpeptidergic NOP receptor agonist, MT-7716 [(R)-2-3-[1-(Acenaphthen-1-yl)piperidin-4-yl]-2-oxo-2,3-dihydro-1H-benzimidazol-1-yl-N-methylacetamide hydrochloride hydrate], and its interaction with ethanol on GABAergic transmission in CeA slices of na?ve rats. We found that MT-7716 dose-dependently (100–1000 nM) diminished evoked GABAA receptor-mediated inhibitory postsynaptic potentials (IPSPs) and increased paired-pulse facilitation (PPF) ratio of these evoked IPSPs, suggesting a presynaptic site of action of the MT-7716 by decreasing GABA release at CeA synapses. The presynaptic action of MT-7716 was also supported by the significant decrease in the frequency of miniature inhibitory postsynaptic currents (mIPSCs) induced by the nociceptin receptor (NOP) agonist. Interestingly, MT-7716 prevented the ethanol-induced augmentation of evoked IPSPs. A putative selective NOP antagonist, [Nphe1]Nociceptin(1–13)NH2, totally prevented the MT-7716-induced inhibition of IPSP amplitudes indicating that MT-7716 exerts its effect through NOPs. These data provide support for an interaction between the nociceptin and GABAergic systems in the CeA and for the anti-alcohol properties of the NOP activation. The development of a synthetic nonpeptidergic NOP receptor agonist such as MT-7716 may represent a useful therapeutic target for alcoholism.
Protective Effects of Many Citrus Flavonoids on Cartilage Degradation Process  [PDF]
Lucia Crascì, Annamaria Panico
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2013, DOI: 10.4236/jbnb.2013.43035
Abstract: The objective of this study was to investigate the effects of many citrus flavanones, such as neoeriocitrin, naringin and neohesperidin, in cartilage degradation. Degenerative joint disease involved degradation of joints, including articular cartilage and subchondral bone. When bone surfaces become less well protected by cartilage, bone may be exposed and damaged. The degradation cartilage is mediated by alteration of the balance between anabolic and catabolic processes, changes in proteolytic enzyme activity, mechanical disruption of the cartilage extracellular matrix (ECM), or a combination of these processes. We examine the capability of neoeriocitrin, naringin and neohesperidin, to inhibit metalloproteinase (MMP)-13, collagenase involved in degradation of cartilage matrix components. Also, we assay the flavonoids effect on reducing of Glycosaminoglycans (GAGs) release, and restore Nitric oxide (NO) levels in explant of human articular cartilage. Our results suggest that neoeriocitrin, naringin and neohesperidin are a potential therapeutic agent to protect cartilage tissue.
Simulating the Seismic Response of Concentrically Braced Frames Using Physical Theory Brace Models  [PDF]
Liang Chen, Lucia Tirca
Open Journal of Civil Engineering (OJCE) , 2013, DOI: 10.4236/ojce.2013.32A008
Abstract:

The aim of this paper is to assess the accuracy of brace models formulated in Drain 2DX and OpenSees by comparing the simulated results with those obtained from experimental tests. Both, Drain 2DX and OpenSees rely on the physical theory brace model. In this study, experimental tests conducted on the behaviour of structural hollow section braces subjected to symmetric and asymmetric quasi-static cyclic loading were selected for calibrating the numerical model. In addition, the predicted failure strain parameter resulted from a proposed empirical equation as a function of slenderness ratio, width-to-thickness ratio and steel properties was used to define the low-cycle fatigue material that was assigned to model braces in OpenSees. It is concluded that both Drain 2DX and OpenSees brace models give a good prediction in terms of maximum tensile and buckling force, as well as interstorey drift. However, in Drain 2DX, the brace model is not able to replicate the out-of-plan buckling and the braced frame model cannot provide an accurate response when the system experiences highly nonlinear demand. To emphasise the differences in performance between Drain 2DX and OpenSees, the behaviour of a 4-storey concentrically braced frame with zipper bracing configuration, located in Victoria, BC, was investigated.

Effects of Water Distribution Uniformity on Waxy Corn (Zea mays L.) Yield: Preliminary Results  [PDF]
Lucia Bortolini, Marco Martello
Journal of Water Resource and Protection (JWARP) , 2014, DOI: 10.4236/jwarp.2014.611098
Abstract:

To face climatic changes, the increase of frequency and severity of droughts, and economic development pressures, Water Management Institutions point at the necessity of water conserving. A sustainable demand-led approach is the most viable option, focusing on achieving more efficient irrigation practices instead of less sustainable expansion of supply. The aim of this work is to investigate on some aspects of the rational water management for the irrigation of corn in order to evaluate the actual possibility of introduction of drip irrigation systems on the Venetian Plain. Trials were carried out on a farm located in Isola della Scala (Verona, Italy) in an area of about 13 ha (12 ha with a drip irrigation system and 1 ha with a solid-set sprinkler system); the effects of the performance of the two systems on waxy corn yield were considered. In particular, water distribution uniformity, water use efficiency, and yield performance of irrigation systems were evaluated. DUlq and CU coefficients for distribution uniformity and IWUe and WUEb indexes for water use efficiency were calculated. To visualize and better analyze the distribution uniformity, the crop yield and the efficiency parameters, ArcGIS 10 (ESRI?) with IDW interpolation were used to create maps. First results show a good water uniformity distribution in the drip irrigation system but only with a careful and proper design of the entire system. As regards the water use efficiency, drip irrigation values are lower than those of sprinkler irrigation (-46% for IWUe and -25% for WUEb), probably due to different irrigation management and water volumes used: in fact, drip irrigation was scheduled every three days, while only supplemental operations were done for sprinkler irrigation. No statistical correlation was found between application rate and yield for both

Virginia Woolf’s History of Sexual Victimization: A Case Study in Light of Current Research  [PDF]
Lucia C. A. Williams
Psychology (PSYCH) , 2014, DOI: 10.4236/psych.2014.510128
Abstract:

Virginia Woolf’s history of sexual victimization is presented in a case study format, and reviewed in light of the present literature on the impact of child sexual abuse (CSA) to human development. The methodology to compose the case study involved reviewing the works of Woolf’s main biographers, the author’s memoirs, and the groundbreaking work of Louise DeSalvo, presenting data from Woolf’s diaries and letters, in which sexual abuse is disclosed. Woolf was sexually abused by her two older half-brothers. The abuse was extremely traumatic, and lasted several years. The various mental health symptoms that Woolf experienced are consistent with the literature of CSA. Woolf also presented some adequate coping skills by disclosing the CSA publicly, keeping records of her depressive episodes, and seeking help. Like many incest survivors, Woolf’s sexual abuse was minimized and questioned by biographers. In addition to Woolf’s enormous literary legacy, her knowledge of psychology was impressive. She was a feminist, as well as a visionary in exploring the effects of CSA before other incest survivors. Understanding her life influences is advantageous, not only to literary scholars but to most readers, and mainly clinicians and researchers are interested in the dynamics of sexual abuse.

Multicriteria Partial Cooperative Games  [PDF]
Graziano Pieri, Lucia Pusillo
Applied Mathematics (AM) , 2015, DOI: 10.4236/am.2015.612186
Abstract: In this paper, we study an approach to environmental topics, through multicriteria partial cooperative games. In general, not all players wish to cooperate to solve a common problem, so we consider a model where only some decision-makers cooperate. Starting from the transformation of a coalition game into a strategic one, we give a new concept of solution for partial cooperative models proving an existence theorem.
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