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Search Results: 1 - 10 of 12410 matches for " Lopamudra Das Roy "
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Exacerbated metastatic disease in a mouse mammary tumor model following latent gammaherpesvirus infection
Vinita S Chauhan, Daniel A Nelson, Lopamudra Das Roy, Pinku Mukherjee, Kenneth L Bost
Infectious Agents and Cancer , 2012, DOI: 10.1186/1750-9378-7-11
Abstract: Mice latently infected with HV-68 had a similar primary tumor burden, but much greater metastatic disease, when compared to mock treated mice given the transplantable tumor, 4?T1. This was true for lung lesions, as well as secondary tumor masses. Increased expression of pan-cytokeratin and VEGF-A in tumors from HV-68 infected mice was consistent with increased metastatic disease in these animals. Surprisingly, no viral particles could be cultured from tumor tissues, and the presence of viral DNA or RNA transcripts could not be detected in primary or secondary tumor tissues.Latent HV-68 infection had no significant effect on the size of primary 4?T1 mammary tumors, but exacerbated the number of metastatic lung lesions and secondary tumors when compared to mock treated mice. Increased expression of the tumor marker, pan-cytokeratin, and VEGF-A in tumors of mice harboring latent virus was consistent with an exacerbated metastatic disease. Mechanisms responsible for this exacerbation are indirect, since no virus could be detected in cancerous tissues.
Breast cancer-associated metastasis is significantly increased in a model of autoimmune arthritis
Lopamudra Das Roy, Latha B Pathangey, Teresa L Tinder, Jorge L Schettini, Helen E Gruber, Pinku Mukherjee
Breast Cancer Research , 2009, DOI: 10.1186/bcr2345
Abstract: To determine if chronic inflammation induced by autoimmune arthritis contributes to increased breast cancer-associated metastasis, we generated mammary gland tumors in SKG mice that were genetically prone to develop AA. Two breast cancer cell lines, one highly metastatic (4T1) and the other non-metastatic (TUBO) were used to generate the tumors in the mammary fat pad. Lung and bone metastasis and the associated inflammatory milieu were evaluated in the arthritic versus the non-arthritic mice.We report a three-fold increase in lung metastasis and a significant increase in the incidence of bone metastasis in the pro-arthritic and arthritic mice compared to non-arthritic control mice. We also report that the metastatic breast cancer cells augment the severity of arthritis resulting in a vicious cycle that increases both bone destruction and metastasis. Enhanced neutrophilic and granulocytic infiltration in lungs and bone of the pro-arthritic and arthritic mice and subsequent increase in circulating levels of proinflammatory cytokines, such as macrophage colony stimulating factor (M-CSF), interleukin-17 (IL-17), interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), and tumor necrosis factor-alpha (TNF-alpha) may contribute to the increased metastasis. Treatment with anti-IL17 + celecoxib, an anti-inflammatory drug completely abrogated the development of metastasis and significantly reduced the primary tumor burden.The data clearly has important clinical implications for patients diagnosed with metastatic breast cancer, especially with regards to the prognosis and treatment options.Metastasis is regulated not only by intrinsic genetic changes in malignant cells, but also by the microenvironment. Several studies have demonstrated that sites of chronic inflammation are often associated with the establishment and growth of various malignancies [1]. A common inflammatory condition in humans is autoimmune arthritis (AA) that causes inflammation and deformity of the
Faculty Development Effectiveness: Insights from a Program Evaluation
Anupma Wadhwa,Lopamudra Das,Savithiri Ratnapalan
Journal of Biomedical Education , 2014, DOI: 10.1155/2014/286081
Abstract: Background. Faculty development programs are often time and resource intensive. In order to accommodate time constrained clinicians a limited time commitment faculty development program was developed and was shown to be effective in improving participant’s scholarly productivity. Objectives. The objective of this study was to assess participants’ perceptions of why the faculty development program was effective in promoting scholarship in education. Methods. In-depth semistructured interviews of course participants were conducted a year after completing a faculty development program. The interviews were audiotaped and transcribed verbatim. The transcriptions were coded independently by the investigators for dominant themes. The investigators held coding meetings to further refine the themes and discrepancies were handled by referring to the transcripts and reaching consensus. Results. The participants’ satisfaction with the course as described in the interviews correlated with the early satisfaction surveys. Reasons offered for this impact fell into four broad categories: course content, course format, social networking during the course, and the course facilitation coaching strategies to achieve goals. Conclusions. Course focusing on the process, experiential learning, and situating the course facilitator in the role of a functional mentor or coach to complete projects can be effective in facilitating behaviour change after faculty development programs. 1. Background Faculty development is seen as an imperative for every medical school [1]; however, there remains a paucity of evidence regarding its effectiveness [2]. Furthermore, there has been a call to evaluate faculty development effectiveness beyond outcomes, that is, to explore why an intervention is effective in order to better consider its applicability and transferability to other contexts [3]. Some “key features” to effective faculty development programs designed to improve teaching effectiveness have been identified [2]. There has been a call, however, for more qualitative studies to explore the complexities of these interventions and for evaluation to include faculty development interventions targeted at other faculty roles such as education scholarship [4]. The production and dissemination of education scholarship are critical to the education mission of Academic Health Science Centers (AHSCs). Such work ensures new knowledge is generated in the field of medical education, it promotes the delivery of high quality medical training through evidence based practices, and it facilitates academic
Differential Effect of Isooctane Doses on HaCaT and HeLa: A Multimodal Analysis
Lopamudra Das,Sanmitra Basu,Sanghamitra Sengupta,Soumen Das,Jyotirmoy Chatterjee
Advances in Toxicology , 2014, DOI: 10.1155/2014/371497
Abstract: A multimodal approach is effective in analyzing biological problems critically and thus also useful in assessing cytotoxicity under chemicals assaults. In this study effects of isooctane, an organic solvent and component of gasoline produced in petroleum industries, have been explored on normal (HaCaT) and cancerous (HeLa) epithelial cells. Besides morphological alterations, impacts on viability, prime molecular expressions, and bioelectrical properties on exposure to different doses of isooctane were noted. Scanning electron microscopy and viability assay demonstrated remarkable structural alterations and cell death, respectively, in HaCaT but not in HeLa. Transcriptomic and immunocytochemical studies on E-cadherin expression also elucidated pronounced toxic effects on HaCaT. Remarkable changes on the bioelectrical properties (e.g., impedance and phase angle) of the HaCaT, in contrast to HeLa, at different temporal points on isooctane exposure also indicated cytotoxic effects in the former. Hence this study illustrated cytotoxicity of isooctane on HaCaT multidimensionally which was evaded by HeLa. 1. Introduction Profuse use of chemicals in modern times is posing serious threats to the health and environment. The number of synthetic substances to which humans are exposed has increased enormously over the last decades as well [1]. In the context of hazards, toxic effect of organic solvents (OS) is a major health concern [2] especially due to their cytotoxicity [3] and protein denaturing effects [3, 4]. Isooctane (IO) is one of such synthetic OS and needs better attention particularly to elucidate its impact on mammalian objects [5]. It is produced on a massive scale in the petroleum industry by distillation of petroleum [6] and is an important component of gasoline. IO is useful for production of ethanol with extremely low volatility [7]. Structurally it has high octane number and does not contain any aromatics, metals, or sulphur. In 2008 the worldwide market of IO was expected to be 1.0 million tons approximately [8]. The toxicity of any OS depends on the cell type and its physiological state [9]. The primary site of cytotoxic action is cytoplasmic membrane of cells [3]. Cytotoxicity of IO on microorganism like yeast has already been reported [10] but its impact on the mammalian cells is not well documented. Only the positive effect of IO in combination with acetic acid in psoriasis is reported [11]. The epithelium is the interface between body and external environment. Its tightly packed cells (namely, keratinocytes) are effective barriers between
Two Rare Anatomical Variations of External Jugular Vein: an Embryological Overview
Ghosh,Susmita; Mandal,Lopamudra; Roy,Sanchita; Bandyopadhyay,Manimay;
International Journal of Morphology , 2012, DOI: 10.4067/S0717-95022012000300008
Abstract: human anatomy is an ocean of unending variations and its mysteries are being unravelled since ages. knowledge of variations in the superficial veins of head and neck is essential to carry out successful surgical procedures. during routine dissection of head and neck of two middle aged male cadavers, in the department of anatomy, calcutta national medical college, kolkata, india, we came out with some interesting venous architecture. the retromandibular vein on the left side of both the cadavers was found to be undivided and joined with the facial vein in the neck at an acute angle to form a common venous trunk at variable distances from the angle of the mandible. that trunk ultimately drained into the left subclavian vein. this might be the result of disappearance of the cephalic part of the external jugular vein and formation of an additional communication between common facial vein and the external jugular vein in foetal life. in one case, we also came across an extra transverse communication between that common trunk and the internal jugular vein. a detail of those cases with embryological explanation is attempted.
Two Rare Anatomical Variations of External Jugular Vein: an Embryological Overview Dos Raras Variaciones Anatómicas de la Vena Yugular Externa: una Visión Embriológica
Susmita Ghosh,Lopamudra Mandal,Sanchita Roy,Manimay Bandyopadhyay
International Journal of Morphology , 2012,
Abstract: Human anatomy is an ocean of unending variations and its mysteries are being unravelled since ages. Knowledge of variations in the superficial veins of head and neck is essential to carry out successful surgical procedures. During routine dissection of head and neck of two middle aged male cadavers, in the Department of Anatomy, Calcutta National Medical College, Kolkata, India, we came out with some interesting venous architecture. The retromandibular vein on the left side of both the cadavers was found to be undivided and joined with the facial vein in the neck at an acute angle to form a common venous trunk at variable distances from the angle of the mandible. That trunk ultimately drained into the left subclavian vein. This might be the result of disappearance of the cephalic part of the external jugular vein and formation of an additional communication between common facial vein and the external jugular vein in foetal life. In one case, we also came across an extra transverse communication between that common trunk and the internal jugular vein. A detail of those cases with embryological explanation is attempted. La anatomía humana tiene un sin fin de variaciones y sus misterios se han develado desde el inicio de los tiempos. El conocimiento de las variaciones anatómicas de las venas superficiales de la cabeza y del cuello es fundamental para llevar a cabo con éxito los procedimientos quirúrgicos. Durante una disección de rutina de cabeza y cuello encontramos una arquitectura venosa interesante en dos cadáveres de hombres de mediana edad en el Departamento de Anatomía de la Facultad de Medicina Nacional Calcuta, Calcuta, India. En el lado izquierdo del cuello de ambos cadáveres, la vena retromandibular no se encontraba dividida, y se unía a la vena facial en un ángulo agudo, para formar un tronco venoso común a una distancia variable desde el ángulo de la mandíbula. Ese tronco finalmente drenaba en la vena subclavia izquierda. Esto podría ser el resultado de la desaparición de la parte cefálica de la vena yugular externa y la formación de una comunicación adicional entre la vena facial común y la vena yugular externa en la vida fetal. En un caso, también se encontró con una comunicación adicional transversal entre ese tronco común y la vena yugular interna. Se realiza una descripción en detalle de los casos junto a una explicación embriológica.
Age changes in the anthropometric and body composition characteristics of the Bishnupriya Manipuris of Cachar district, Assam  [PDF]
Bhubon Mohan Das, Subrata K. Roy
Advances in Bioscience and Biotechnology (ABB) , 2010, DOI: 10.4236/abb.2010.12017
Abstract: Generally all morphological physical features of human body change throughout ones life span, the rates of change are not constant and there are certain ages when anthropometric characteristics reach its peak. The maturity of the anthropometric characteristics varies among populations/communities and sexes due to genetic make-up, environment and other concomitants. Present study examined the nature and extent of age related changes in anthropometric and body composition characteristics among the adult Bishnupriya Manipuris of Cachar district, Assam. Some anthropometric data and body composition characteristics analysed and compared between/among 5age cohorts to see age changes. Study results show significant age changes in some anthropometric characteristics and some body composition characteristics. However, age specific changes could not been determined due to small sample size.
Characterization of a Highly Potent Insecticidal Lectin from Colocasia esculenta Tuber and Cloning of Its Coding Sequence  [PDF]
Ayan Das, Amit Roy, Daniel Hess, Sampa Das
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.42A053
Abstract:

Hemipteran insects are the most devastating pest for different crops of high economic value. Colocasia esculenta tuber agglutinin (CEA), a mannose binding monocot lectin from araceae family was previously reported by the present group to be effective against some members of this class of pests. In the present study, efficacy of this potent lectin has been extended to cotton aphid (Aphis gossypii) which is becoming a highly damaging pest of cotton in recent days. Because, like other aphids, A. gossypii not only extracts the phloem fluid but also transmit disease causing viruses and add to the high degree of yield loss. Efficacy of the lectin on cotton aphid as well as other hemipteran insects prompted us further to clone the protein coding gene. Very little sequence information of this gene was available in the database. Hence, attempt had been made to study the protein through liquid chromatography-tandem mass spectrometry (LC-MS/MS) to have the detailed peptide information. On the basis of the peptide homology information obtained from LC-MS/MS the complete coding sequence of CEA was determined. The coding sequence corresponding to CEA was cloned further using primers designed on the basis of above information and genome walk technology for its potential utilisation in insect management programme.

Collagen induced arthritis increases secondary metastasis in MMTV-PyV MT mouse model of mammary cancer
Lopamudra Roy, Sriparna Ghosh, Latha B Pathangey, Teresa L Tinder, Helen E Gruber, Pinku Mukherjee
BMC Cancer , 2011, DOI: 10.1186/1471-2407-11-365
Abstract: To establish the novel link between arthritis induced inflammation and secondary metastasis associated with breast cancer, PyV MT mice that spontaneously develop mammary gland carcinoma were injected with Type II collagen (CII) to induce arthritis at 9 and 18 weeks of age for pre-metastatic and metastatic condition. The sites of secondary metastasis and the associated inflammatory microenvironment were evaluated.A significant increase in breast cancer-associated secondary metastasis to the lungs and bones was observed in the arthritic versus the non-arthritic PyV MT mice along with an increase in primary tumor burden. We report significant increases in the levels of interstitial cellular infiltrates and pro-inflammatory cytokines such as interleukin-17 (IL-17), interleukin-6 (IL-6), Pro- Matrix metallopeptidase 9 (Pro-MMP9), insulin like growth factor-II (GF-II) and macrophage colony stimulating factor (M-CSF) in the arthritic lung and bone milieu as well as in the circulation. These pro-inflammatory cytokines along with the inflammatory microenvironment may be the underlying factors facilitating tumor progression and metastasis in arthritic PyV MT mice. This was further substantiated by treatment with celecoxib, an anti-inflammatory drug + αIL-17 antibody that significantly reduced the secondary metastasis to lung and bone.The data generated not only reveal the underlying mechanism of high susceptibility to bone and lung metastasis in an arthritic condition but our combination therapies may lead to treatment modalities that will be capable of reducing tumor burden, and preventing relapse and metastasis in arthritic patients with breast cancer.While advances have been made in breast cancer therapies, metastatic breast cancer remains an incurable disease, and thus the prevention of metastases must be a priority. The preference of breast cancer cells to grow in the bone and lung is underscored by the fact that 65-75% of patients with advanced disease develop metastasi
The Zero Curvature Formulation of TB, sTB Hierarchy and Topological Algebras
Ashok Das,Shibaji Roy
Physics , 1995, DOI: 10.1142/S0217732396001326
Abstract: A particular dispersive generalization of long water wave equation in $1+1$ dimensions, which is important in the study of matrix models without scaling limit, known as two--Boson (TB) equation, as well as the associated hierarchy has been derived from the zero curvature condition on the gauge group $SL(2,R)\otimes U(1)$. The supersymmetric extension of the two--Boson (sTB) hierarchy has similarly been derived from the zero curvature condition associated with the gauge supergroup $OSp(2|2)$. Topological algebras arise naturally as the second Hamiltonian structure of these classical integrable systems, indicating a close relationship of these models with 2d topological field theories.
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