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Search Results: 1 - 10 of 333634 matches for " Livia S Machado "
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Delayed minocycline inhibits ischemia-activated matrix metalloproteinases 2 and 9 after experimental stroke
Livia S Machado, Anna Kozak, Adviye Ergul, David C Hess, Cesario V Borlongan, Susan C Fagan
BMC Neuroscience , 2006, DOI: 10.1186/1471-2202-7-56
Abstract: Both MMP-2 and MMP-9 were elevated in the ischemic tissue as compared to the contra-lateral hemisphere after 3 hours occlusion and 21 hours survival (p < 0.0001 for MMP-9). Intraperitoneal minocycline at 45 mg/kg concentration twice a day (first dose immediately after the onset of reperfusion) significantly reduced gelatinolytic activity of ischemia-elevated MMP-2 and MMP-9 (p < 0.0003). Treatment also reduced protein concentration of both enzymes (p < 0.038 for MMP-9 and p < 0.018 for MMP-2). In vitro incubation of minocycline in concentrations as low as 0.1 μg/ml with recombinant MMP-2 and MMP-9 impaired enzymatic activity and MMP-9 was more sensitive at lower minocycline concentrations (p < 0.05).Minocycline inhibits enzymatic activity of gelatin proteases activated by ischemia after experimental stroke and is likely to be selective for MMP-9 at low doses. Minocycline is a potential new therapeutic agent to acute treatment of ischemic stroke.Matrix metalloproteases (MMPs) are a family of zinc dependent proteases responsible for the extracellular matrix turnover and degradation of bioactive proteins. In cerebral ischemia, MMPs 2 and 9, also designated as gelatinases A (72 kDa) and B (92 kDa) have been identified to mediate the degradation of the basal lamina [1,2] and hemorrhagic transformation [3]. MMPs 2 and 9 have been shown to be elevated a few hours after ischemia [4] and to maintain increased activity for days after the onset [2,5]. The inhibition of these enzymes by specific class inhibitors reverts breakdown of laminin [6] and prevents increased barrier permeability, edema, and hemorrhage after ischemic stroke [7-9]. The development of MMP inhibitors as therapeutic agents has been limited by their poor solubility.Minocycline is a commonly used semi-synthetic tetracycline with anti-inflammatory and anti-apoptotic properties [10,11]. Minocycline interferes with MMP activity [12,13] and has been shown to be neuroprotective in cerebral ischemia [14] and in oth
Effect of neutrophil depletion on gelatinase expression, edema formation and hemorrhagic transformation after focal ischemic stroke
Alex K Harris, Adviye Ergul, Anna Kozak, Livia S Machado, Maribeth H Johnson, Susan C Fagan
BMC Neuroscience , 2005, DOI: 10.1186/1471-2202-6-49
Abstract: Anti-PMN treatment caused successful depletion of neutrophils in treated animals. There was no difference in either infarct volume or hemorrhage between control and PMN depleted animals. While there were significant increases in gelatinase (MMP-2 and MMP-9) expression and activity and edema formation associated with ischemia, neutrophil depletion failed to cause any change.The main finding of this study is that, in the absence of circulating neutrophils, MMP-2 and MMP-9 expression and activity are still up-regulated following focal cerebral ischemia. Additionally, neutrophil depletion had no influence on indicators of ischemic brain damage including edema, hemorrhage, and infarct size. These findings indicate that, at least acutely, neutrophils are not a significant contributor of gelatinase activity associated with acute neurovascular damage after stroke.The matrix metalloproteinases (MMPs) are a family of some 23 zinc dependent proteases that, collectively, possess the ability to degrade nearly every component of the extra-cellular matrix [1-3]. The activity of the MMPs is tightly controlled through proteolytic activation of the zymogen forms and stoichiometric binding of tissue inhibitors of metalloproteinases (TIMPs). The MMPs play an important role in many physiological processes due to their inherent ability to remodel tissues [2,3]. However, in disease states such as vascular disease and stroke, the MMPs may become deleterious due to dysregulation and can result in tissue injury and inflammation. Specifically, the MMPs may be involved in the degradation of the basal lamina in reperfusion injury resulting in disruption of the blood brain barrier and hemorrhagic transformation [4].Recently, several lines of evidence have demonstrated the involvement of the MMPs in cerebral ischemia. Studies in rat, mouse, and baboon models have shown that MMP-9 is up-regulated following transient focal ischemia [5-8]. Additionally, Asahi et al. have shown that MMP-9 knockout as
Surgery for inflammatory bowel disease in the era of laparoscopy
Giuseppe S Sica,Livia Biancone
World Journal of Gastroenterology , 2013, DOI: 10.3748/wjg.v19.i16.2445
Abstract: During the course of inflammatory bowel disease (IBD), surgery may be needed. Approximately 20% of patients with ulcerative colitis (UC) will require surgery, whereas up to 80% of Crohn’s disease (CD) patients will undergo an operation during their lifetime. For UC patients requiring surgery, total proctocolectomy and ileoanal pouch anastomosis (IPAA) is the operation of choice as it provides a permanent cure and good quality of life. Nevertheless a permanent stoma is a good option in selected patients, especially the elderly. Minimally invasive surgery has replaced the conventional open approach in many specialized centres worldwide. Laparoscopic colectomy and restorative IPAA is rapidly becoming the standard of care in the treatment of UC requiring surgery, whilst laparoscopic ileo-cecal resection is already the new gold standard in the treatment of complicated CD of terminal ileum. Short term advantages of laparoscopic surgery includes faster recovery time and reduced requirement for analgesics. It is, however, in the long term that minimally invasive surgery has demonstrated its superiority over the open approach. A better cosmesis, a reduced number of incisional hernias and fewer adhesions are the long term advantages of laparoscopy in IBD surgery. A reduction in abdominal adhesions is of great benefit when a second operation is needed in CD and this influences positively the pregnancy rate in young women undergoing restorative IPAA. In developing the therapeutic plan for IBD patients it should be recognized that the surgical approach to the abdomen has changed and that surgical treatment of complicated IBD can be safely performed with a true minimally invasive approach with great patient satisfaction.
Ubiquitous overexpression of a transgene encoding the extracellular portion of the Drosophila Roughest-Irregular Chiasm C protein induces early embryonic lethality
MODA, LIVIA;MACHADO, RICARDO C.;RAMOS, RICARDO GUELERMAN P.;
Anais da Academia Brasileira de Ciências , 2000, DOI: 10.1590/S0001-37652000000300011
Abstract: the cell adhesion molecule rst-irrec is a transmembrane glycoprotein of the immunoglobulin superfamily involved in several important developmental processes in drosophila, including axonal pathfinding in the optic lobe and programmed cell death and pigment cell differentiation in the pupal retina. as an initial step towards the "in vivo'' functional analysis of this protein we have generated transgenic fly stocks carrying a truncated cdna construct encoding only the extracellular domain of rst-irrec under the transcriptional control of the heat shock inducible promoter hsp70. we show that heat-shocking embryos bearing the transgene during the first 8hs of development lead to a 3-4 fold reduction in their viability compared to wild type controls. the embryonic lethality can already be produced by applying the heat pulse in the first 3hs of embryonic development, does not seem to be suppressed in the absence of wildtype product and is progressively reduced as the heat treatment is applied later in embryogenesis. these results are compatible with the hypothesis of the lethal phenotype being primarily due to heterophilic interactions between rst-irrec extracellular domain and an yet unknown ligand.
Ubiquitous overexpression of a transgene encoding the extracellular portion of the Drosophila Roughest-Irregular Chiasm C protein induces early embryonic lethality
MODA LIVIA,MACHADO RICARDO C.,RAMOS RICARDO GUELERMAN P.
Anais da Academia Brasileira de Ciências , 2000,
Abstract: The cell adhesion molecule Rst-irreC is a transmembrane glycoprotein of the immunoglobulin superfamily involved in several important developmental processes in Drosophila, including axonal pathfinding in the optic lobe and programmed cell death and pigment cell differentiation in the pupal retina. As an initial step towards the "in vivo'' functional analysis of this protein we have generated transgenic fly stocks carrying a truncated cDNA construct encoding only the extracellular domain of Rst-IrreC under the transcriptional control of the heat shock inducible promoter hsp70. We show that heat-shocking embryos bearing the transgene during the first 8hs of development lead to a 3-4 fold reduction in their viability compared to wild type controls. The embryonic lethality can already be produced by applying the heat pulse in the first 3hs of embryonic development, does not seem to be suppressed in the absence of wildtype product and is progressively reduced as the heat treatment is applied later in embryogenesis. These results are compatible with the hypothesis of the lethal phenotype being primarily due to heterophilic interactions between Rst-IrreC extracellular domain and an yet unknown ligand.
Anomalías dentó maxilares y factores asociados en ni?os con parálisis cerebral
BARRIONUEVO N,LIVIA; SOLíS F,FRESIA;
Revista chilena de pediatría , 2008, DOI: 10.4067/S0370-41062008000300005
Abstract: background: patients with cerebral palsy attending the children rehabilitation institute (teleton) at santiago-chile present open bite and oral disfunctions. objective: determine dento-maxillary anomalies related to cerebral palsy and factors associated to feeding, breathing and parafunctional habits. method: descriptive study including 80 children of both sex, between 4-12 years-old, with cerebral palsy. a revisión of clinical files, extra and intraoral exams and a parents investigation was performed. results: 83% of children have severe spastic tetraparesis. a high incidence of open bite incisors was found, long faces compatible with dolicofacial growing and labial incompetence. associated to this facial biotype, mixed breathing with bucal predominance and absence of lateral movements were found in these children. the intermaxillary relation is type i and the angle's classification is neutrón occlusion. in relation to feeding habits, 50% of patiens with tetraparesis eat their food cut in very small pieces, whereas 60% with diplegia and hemiparesia eat their food in a normal way. the parafunctional habits were significant (47%). conclusions: cerebral palsy is an important condition in the development of a dento-maxillary disarmony, like open bite and dysfunctional sthomatognatic system
Recuperación de respuestas: una revisión de la evidencia y del modelo de recuperación de información
Sánchez-Carrasco, Livia;Nieto, Javier;
Revista mexicana de análisis de la conducta , 2009,
Abstract: response retrieval phenomena (i.e., context renewal, reinstatement and spontaneous recovery) suggest that despite reduction in responding, the associations established during acquisition are left intact by extinction. this paper presents a review of evidence related to these phenomena, and introduces bouton's information retrieval model that proposes that the context may be seen as an occasion setter. then, some shortcomings of the information retrieval model are discussed, and the attentional theory of context processing is presented, this theory proposes that attention to contextual cues promotes context dependency of information. finally, we present some considerations that must be incorporated into these models and in the ongoing research in the area.
Anomalías dentó maxilares y factores asociados en ni os con parálisis cerebral Dento-maxillary analysis and related factors in children with Cerebral Palsy
LIVIA BARRIONUEVO N,FRESIA SOLíS F
Revista chilena de pediatría , 2008,
Abstract: Introducción: Los pacientes con parálisis cerebral atendidos en el Instituto de Rehabilitación Infantil de Santiago, presentan mordidas abiertas y disfunción oral. Objetivos: Determinar anomalías dentó maxilares y relacionarlas con diagnóstico de parálisis cerebral y factores asociados de alimentación, respiración y hábitos parafuncionales. Pacientes y Método: Estudio descriptivo transversal, que incluyó revisión de fichas clínicas 2005-2006, examen extra e intraoral y encuesta a los padres de 80 ni os, ambos sexos, con diagnóstico de parálisis cerebral entre 4 y 12 a os de edad. Resultados: 83% de los ni os, presentó tetraparesia espástica severa. Se encontró alta incidencia de mordida abierta anterior, caras alargadas compatibles con crecimiento dolicofacial e incompetencia labial. Asociado a este biotipo facial, se halló respiración mixta de predominio bucal y ausencia de movimientos de lateralidad. La relación intermaxilar es tipo I y neutro oclusión según clasificación de Angle. De los hábitos de alimentación, el 50% de los pacientes con tetraparesia comen picado; alrededor del 60% con diplejia y hemiparesia comen entero. Los hábitos parafuncionales no fueron significativos, 47% de los pacientes encuestados los tenían. Conclusiones: La parálisis cerebral es una variable importante asociada al desarrollo de una desarmonía dentomaxilar como la mordida abierta y de la disfunción del sistema estomatognático Background: Patients with cerebral palsy attending the Children Rehabilitation Institute (Teleton) at Santiago-Chile present open bite and oral disfunctions. Objective: Determine dento-maxillary anomalies related to cerebral palsy and factors associated to feeding, breathing and parafunctional habits. Method: Descriptive study including 80 children of both sex, between 4-12 years-old, with cerebral palsy. A revisión of clinical files, extra and intraoral exams and a parents investigation was performed. Results: 83% of children have severe spastic tetraparesis. A high incidence of open bite incisors was found, long faces compatible with dolicofacial growing and labial incompetence. Associated to this facial biotype, mixed breathing with bucal predominance and absence of lateral movements were found in these children. The intermaxillary relation is type I and the Angle's classification is neutrón occlusion. In relation to feeding habits, 50% of patiens with tetraparesis eat their food cut in very small pieces, whereas 60% with diplegia and hemiparesia eat their food in a normal way. The parafunctional habits were significant (47%). Conclusions: Cerebr
A Study on Ethanolysis and Methanolysis of Coconut Oil for Enzymatically Catalyzed Production of Biodiesel  [PDF]
Livia M. O. Ribeiro, Albanise E. Silva, Margarete C. S. Silva, Renata M. R. G. Almeida
Journal of Sustainable Bioenergy Systems (JSBS) , 2014, DOI: 10.4236/jsbs.2014.44020
Abstract: Biodiesel production by enzymatic catalysis has been the subject of much research for developing processes that can potentially compete with other types of catalysis. The objective of this study was to investigate the variables that influenced the transesterification of coconut oil catalyzed by immobilized lipase for biodiesel production. A full 24 factorial design with the variables tempera-ture (40°C - 60°C), enzyme concentration (3% - 7%), oil-ethanol ratio (1:6 - 1:10) and alcohol type (methanol-ethanol) was performed. The best conversion result (80.5%) was obtained using ethanol with a higher temperature, molar ratio and enzyme concentration. The obtained yields showed that the results attained with ethanol were more significant when compared with methanol.
Cirurgia bariátrica: como e por que suplementar
Bordalo, Livia Azevedo;Teixeira, Tatiana Fiche Sales;Bressan, Josefina;Mour?o, Denise Machado;
Revista da Associa??o Médica Brasileira , 2011, DOI: 10.1590/S0104-42302011000100025
Abstract: patients who have undergone bariatric surgery are at increased risk of developing nutritional deficiencies from limited food intake and absorption of different nutrients. a systematic review of several database websites (pubmed and isi web of science) was conducted from september 1983 to april 2010 to identify literature related to micronutrient deficiencies occurring after bariatric surgery. keywords used individually or in various combinations in the search were bariatric surgery, obesity, vitamin/mineral deficiencies, protein deficiency, nutrient absorption and nutrient supplementation. literature suggests that to prevent or treat nutritional deficiencies resulting from anatomical changes due to surgical techniques nutritional supplementation is usually necessary. the success of oral nutritional supplementation to correct or prevent nutritional deficiencies depends on several factors. thus, to understand how nutrients can be administered is very important for clinical practice. this review aims to provide help for the best selection of nutrients to ensure an adequate replacement of nutrients in patients who have undergone bariatric surgery.
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