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Search Results: 1 - 10 of 120128 matches for " Linfang Wang "
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Effects of Methyl Jasmonate on the Composition of Volatile Compounds in Pyropia yezoensis Effects of Methyl Jasmonate on the Composition of Volatile Compounds in Pyropia yezoensis
HE Lihong,WANG Liang,WANG Linfang,SHEN Songdong
- , 2018,
Abstract: Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain largely unknown. Here we studied the composition of volatile compouds in Pyropia yezoensis and their variations in response to methyl jasmonate(MeJA) and diethyldithiocarbamic acid(DIECA) treatment using gas chromatography-mass spectrometry(GC-MS). A total of 44 compounds belonging to the following chemical classes(n) were identified, including aldehydes(11), alcohols(8), acids and esters(6), alkanes(5), ketones(5), alkenes(3), and S-or N-containing miscellaneous compounds(6). External treatment with plant hormone MeJA increased the content of 1-dodecanol, 4-heptenal, and 2-propenoic acid-2-methyl dodecylester, but decreased the content of phytol, 3-heptadecene, 2-pentadecanone, and isophytol. When pretreated with DIECA, an inhibitor of the octadecanoid pathway leading to the biosynthesis of endogeneous jasmonates and some secondary metabolites, phytol and isophytol were increased, while 4-heptenal, 1-dodecanol, and 2-propenoic acid-2-methyl dodecylester were decreased, both of which were negatively correlated with their variations under MeJA treatment. Collectively, these results suggest that MeJA does affect the volatile composition of P. yezoensis, and the octadecanoid pathway together with endogenous jasmonate pathway may be involved in the biosynthesis of volatile compounds, thereby providing some preliminary envision on the composition and biogeneration of volatile compounds in P. yezoensis
Studies on the relationship between cyanide-resistant respiration and expression of alternative oxidase in mung bean using antibodies prepared by synthetic polypeptide
Chijun Li,Houguo Liang,Linfang Du,Rui Wang
Science China Life Sciences , 2001, DOI: 10.1007/BF02882074
Abstract: Twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of the alternative oxidase (AOX), were synthesized by solid-phase method. The polypeptide was coupled with α-chymotrypsinogen, and the antibodies were obtained through immunizing domestic rabbit by injecting this complex. By using these antibodies, which were raised to immunoreact with total proteins of purified mitochondria from different organs of mung bean seedlings, we find that there are two hybridizable AOX bands in mitochondria. Their molecular weights are about 35 and 38 ku, respectively. Moreover, the respiratory parameters of hypocotyl, true leaf and cotyledon of mung bean seedlings show that true leaf has the highest total respiration (Vt), alternative pathway (AP) capacity (Valt) and the activity of AP (ρValt) among the three organs. Vt andρV alt of cotyledon ranked the second. Hypocotyl has the lowest Vt andρV alt, but its Valt is higher than that of cotyledon. These results are consistent with the analysis of Western blotting of expression of AOX. The highest Vt andρV alt in true leaf are accompanied two hybridizable polypeptides of AOX protein, 35 ku and 38 ku respectively. The next is cotyledon Vt andρV alt with only one 38 ku hybridizable polypeptide of AOX protein. HypocotylV t andρV alt is the lowest and its immunoblotting band is similar to that of cotyledon, but the expressive amount of 38 ku protein is less than that of cotyledon. The results suggest that the 35 ku AOX may contribute mainly to true leafρV alt.
Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding
Sihao Zheng, Xue Jiang, Labin Wu, Zenghui Wang, Linfang Huang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0098061
Abstract: Cistanches Herba (Rou Cong Rong), known as “Ginseng of the desert”, has a striking curative effect on strength and nourishment, especially in kidney reinforcement to strengthen yang. However, the two plant origins of Cistanches Herba, Cistanche deserticola and Cistanche tubulosa, vary in terms of pharmacological action and chemical components. To discriminate the plant origin of Cistanches Herba, a combined method system of chemical and genetic –UPLC-QTOF/MS technology and DNA barcoding–were firstly employed in this study. The results indicated that three potential marker compounds (isomer of campneoside II, cistanoside C, and cistanoside A) were obtained to discriminate the two origins by PCA and OPLS-DA analyses. DNA barcoding enabled to differentiate two origins accurately. NJ tree showed that two origins clustered into two clades. Our findings demonstrate that the two origins of Cistanches Herba possess different chemical compositions and genetic variation. This is the first reported evaluation of two origins of Cistanches Herba, and the finding will facilitate quality control and its clinical application.
Implementation of the No Packet Loss Network Communication in VxWorks
Linfang Zhang
Modern Applied Science , 2010, DOI: 10.5539/mas.v4n9p30
Abstract: By VxWorks, the real-time control system of certain equipment adopts the UDP/IP network communication, which requires that the control process has strong real-time character and complex task scheduling. In the work condition with frequently interactive network tasks, the control system is easy to lose packets, and to solve this problem, this article adopts the double-loop buffer structure processing mechanism to realize the no packet loss network communication method, which could largely enhance the reliability of the network communication and improve the stability of the control system. Based on the excellent multi-task scheduling ability of VxWorks, this method could essentially enhance the efficiency and reliability of software. By testing, this method is efficient and feasible.
Exosome-Related Multi-Pass Transmembrane Protein TSAP6 Is a Target of Rhomboid Protease RHBDD1-Induced Proteolysis
Chunhua Wan, Jun Fu, Yong Wang, Shiying Miao, Wei Song, Linfang Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0037452
Abstract: We have previously reported that rhomboid domain containing 1 (RHBDD1), a mammalian rhomboid protease highly expressed in the testis, can cleave the Bcl-2 protein Bik. In this study, we identified a multi-pass transmembrane protein, tumor suppressor activated pathway-6 (TSAP6) as a potential substrate of RHBDD1. RHBDD1 was found to induce the proteolysis of TSAP6 in a dose- and activity-dependent manner. The cleavage of TSAP6 was not restricted to its glycosylated form and occurred in three different regions. In addition, mass spectrometry and mutagenesis analyses both indicated that the major cleavage site laid in the C-terminal of the third transmembrane domain of TSAP6. A somatic cell knock-in approach was used to genetically inactivate the endogenous RHBDD1 in HCT116 and RKO colon cancer cells. Exosome secretion was significantly elevated when RHBDD1 was inactivated in the two cells lines. The increased exosome secretion was verfied through the detection of certain exosomal components, including Tsg101, Tf-R, FasL and Trail. In addition, the elevation of exosome secretion by RHBDD1 inactivation was reduced when TSAP6 was knocked down, indicating that the role of RHBDD1 in regulating exosomal trafficking is very likely to be TSAP6-dependent. We found that the increase in FasL and Trail increased exosome-induced apoptosis in Jurkat cells. Taken together, our findings suggest that RHBDD1 is involved in the regulation of a nonclassical exosomal secretion pathway through the restriction of TSAP6.
GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules
Yong Wang, Wei Song, Shuchun Li, Xin Guan, Shiying Miao, Shudong Zong, SS Koide, Linfang Wang
BMC Cell Biology , 2009, DOI: 10.1186/1471-2121-10-25
Abstract: Stable mRHBDD1 knockdown GC-1 cells were sensitive to apoptotic stimuli, PS341 and UV irradiation. In vitro, they survived and proliferated normally. However, they lost the ability to survive and differentiate in mouse seminiferous tubules.Our findings suggest that mRHBDD1 may be associated with mammalian spermatogenesis.Spermatogenesis generates functional sperm cells from initially undifferentiated germ cells. This involves the proliferation of spermatogonia, meiosis of spermatocytes and the differentiation of spermatids into spermatozoa. It is a complex developmental program in which myriad events take place to ensure that the germ cells reach their proper stages of development at the appropriate times. Normal spermatogenesis requires a well-regulated balance of several processes, including cell proliferation, differentiation and apoptosis.Apoptosis is a key phenomenon during spermatogenesis. For instance, an early, massive wave of germ cell apoptosis occurs at puberty. This event takes place during postnatal weeks 2 to 4 in mice, with a peak after 3 weeks [1-3]. It is estimated that 75% of spermatogenic cells undergo apoptosis during development [4,5], ensuring the maintenance of a critical ratio between maturing germ cells and Sertoli cells [2,6]. Sporadic apoptosis also occurs, primarily in spermatogonia and spermatocytes [2], eliminating defective germ cells with mutated DNA [7].The Rhomboid family comprises polytopic membrane proteins, which may be the most widely-conserved membrane proteins identified to date [8]. They share conserved biochemical properties in all biological kingdoms. Rhomboid proteases, which have been well studied in Drosophila, appear to regulate EGF receptor signalling pathways, thereby controlling growth and development [9,10]. In addition, some yeast Rhomboid proteases have been found to play important roles in mitochondrial membrane remodelling [11], while some parasite proteases containing a Rhomboid domain are important for invasiv
Small Kinetochore Associated Protein (SKAP) Promotes UV-Induced Cell Apoptosis through Negatively Regulating Pre-mRNA Processing Factor 19 (Prp19)
Shan Lu, Renxian Wang, Congli Cai, Junbo Liang, Longchang Xu, Shiying Miao, Linfang Wang, Wei Song
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0092712
Abstract: Apoptosis is a regulated cellular suicide program that is critical for the development and maintenance of healthy tissues. Previous studies have shown that small kinetochore associated protein (SKAP) cooperates with kinetochore and mitotic spindle proteins to regulate mitosis. However, the role of SKAP in apoptosis has not been investigated. We have identified a new interaction involving SKAP, and we propose a mechanism through which SKAP regulates cell apoptosis. Our experiments demonstrate that both overexpression and knockdown of SKAP sensitize cells to UV-induced apoptosis. Further study has revealed that SKAP interacts with Pre-mRNA processing Factor 19 (Prp19). We find that UV-induced apoptosis can be inhibited by ectopic expression of Prp19, whereas silencing Prp19 has the opposite effect. Additionally, SKAP negatively regulates the protein levels of Prp19, whereas Prp19 does not alter SKAP expression. Finally, rescue experiments demonstrate that the pro-apoptotic role of SKAP is executed through Prp19. Taken together, these findings suggest that SKAP promotes UV-induced cell apoptosis by negatively regulating the anti-apoptotic protein Prp19.
The Amphiphilic Self-Assembling Peptide EAK16-I as a Potential Hydrophobic Drug Carrier
Jing Wang,Fushan Tang,Feng Li,Juan Lin,Yinghui Zhang,Linfang Du,Xiaojun Zhao
Journal of Nanomaterials , 2008, DOI: 10.1155/2008/516286
Abstract: It is crucial for hydrophobic drugs to be dissolved and stabilized by carriers in aqueous systems and then to be delivered into target cells. An amphiphilic self-assembling peptide EAK16-I (Ac-AEAKAEAKAEAKAEAK-NH2) is reported here to be able to stabilize a model hydrophobic compound, pyrene, in aqueous solution, resulting in the formation of colloidal suspensions. Egg phosphatidylcholine (EPC) vesicles are used as plasma membranes mimic. Fluorescence data shows that the pyrene is presented in the crystalline form when stabilized by EAK16-I and molecularly migrates from its peptide encapsulations into the membrane bilayers of EPC vesicles when the suspension is mixed with EPC vesicles. Furthermore, the release rate can be controlled by changing peptide-to-pyrene ratio, and the higher ratios lead to the slower release rates due to a thicker encapsulation on the pyrene microcrystals. This demonstrates that EAK16-I, as a promising nanobiomaterial, has the potential to be a hydrophobic compounds carrier.

Yang Linfang,Zheng Xinqi,Wang Youbang,

资源科学 , 1996,
Abstract: he contradiction caused due to population pressure and shortage of land has become increasingly obvious in Shandong Province. Shandong is advantageous in aquatic products, hence the exploitation of fishery resources and turning the potential superiority of resources into economic superiority would be the breakthrough to the solution of this contradiction. In this paper, the author analyzes the resources superiority, development situations and existing problems as well as explores methods and countermeasures effective for integrated development of the province.
The Protective Effect of Bcl-xl Overexpression against Oxidative Stress-Induced Vascular Endothelial Cell Injury and the Role of the Akt/eNOS Pathway
Leng Ni,Tianjia Li,Bao Liu,Xitao Song,Genhuan Yang,Linfang Wang,Shiying Miao,Changwei Liu
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141122149
Abstract: Restenosis after intraluminal or open vascular reconstruction remains an important clinical problem. Vascular endothelial cell (EC) injury induced by oxidative stress plays an important role in the development of intimal hyperplasia. In this study, we sought to evaluate the protective effects of Bcl-xl overexpression in vitro on oxidative stress-induced EC injury and the role of the Akt/endothelial nitric oxide synthase (eNOS) pathway. Human umbilical vein endothelial cells (HUVECs) exposed to hydrogen peroxide (H 2O 2, 0.5 mM) were used as the experimental oxidative stress model. The Bcl-xl gene was transferred into HUVECs through recombinant adenovirus vector pAdxsi-GFP- Bcl-xl before oxidative treatment. Cell apoptosis was evaluated by Annexin V/propidium iodide and Hoechst staining, caspase-7 and PARP cleavage. Cell viability was assessed using the cell counting kit-8 assay, proliferating cell nuclear antigen (PCNA) immunocytochemical detection and the scratching assay. Expressions of Akt, phospho-Akt and eNOS were detected by Western blotting. Our results showed that H 2O 2 induced apoptosis and decreased the cell viability of HUVECs. Bcl-xl overexpression significantly protected cells from H 2O 2-induced cell damage and apoptosis and maintained the cell function. Furthermore, the level of phospho-Akt and eNOS protein expression was significantly elevated when pretreated with Bcl-xl gene transferring. These findings suggest that Bcl-xl overexpression exerts an anti-apoptotic and protective effect on EC function. The Akt/eNOS signaling pathway is probably involved in these processes.
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