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Search Results: 1 - 10 of 4297 matches for " Lijuan Qu "
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Protein Globule Formation in the Liver Graft during Cold Preservation for Liver Transplantation: Its Clinical and Pathological Significance  [PDF]
Changyin Feng, Tongmei He, Haijian Huang, Lijuan Qu, Yi Jiang, Wenhua Zhang, Zhiyong Zheng
Open Journal of Pathology (OJPathology) , 2013, DOI: 10.4236/ojpathology.2013.33022
Abstract:

In liver transplantation, liver graft ischemia-reperfusion injury occurs mainly due to cold preservation and warm reperfusion. In this research, we study the affection of plasma protein of the donor on liver graft during cold preservation and warm reperfusion. In this study, 34 liver transplantations were performed from 2007 to 2010, and the clinical data were collected retrospectively from the Dongfang Hospital database. 34 specimens were harvested from 34 liver grafts when graft trimming as Group A and 34 specimens harvested from the same 34 liver grafts during liver transplantation surgery but before abdominal closure as B group. All liver tissue specimens were fixed with 40 g/L neutral formalin, embedded in paraffin. Light microscopy, transmission electron microscopy, Periodic Acid-Schiff (PAS) stain and immunohistochemical stain of IgG, IgM, IgA, C3d, C4d, Fib, C1q, and CD61 were used. In this study, we found that eosinophilic bodies emerged in liver lobes during liver transplantation which had not been reported previously in the literature. 1) Protein globules were found exclusively in liver graft specimens. The globules were round or oval with sharp edges, measured approximately 1.59 to 9.41 μm in diameter, and were scattered in the liver sinusoids or space of Disse or hepatocyte cytoplasm, were stained with IgG, IgM, IgA, Fib, C3d by immunohistochemical staining; 2) There was no statistical significant difference of protein globules number between A group and B group (P > 0.05); 3) IRI score of B group was not correlated with protein globules number (P > 0.05). Protein globules contain plasma composition, and may form during cold preservation.

Detection of A. phagocytophilum and E. chaffeensis in Patient and Mouse Blood and Ticks by a Duplex Real-Time PCR Assay
Tuo Dong, Zhangyi Qu, Lijuan Zhang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074796
Abstract: Human granulocytic anaplasmosis (HGA) and human monocytic ehrlichiosis (HME) are emerging, tick-borne, zoonotic infectious diseases caused by Anaplasma phagocytophilum and Ehrlichia chaffeensis, respectively. Early diagnosis is essential for rapid clinical treatment to avoid misdiagnosis and severe patient outcomes. Simple, sensitive and reliable diagnostic methods are urgently needed. In this study, we developed a duplex real-time PCR assay targeting the A. phagocytophilum ankA gene and the E. chaffeensis TRP120 gene, respectively. The lowest limit of detection of the duplex real-time PCR assay was 100 copies of the targeted A. phagocytophilum ankA gene and the E. chaffeensis TRP120 gene per reaction, and the specificity was 100%. Detection in blood DNA samples from the acute stage of illness for 22 HGA cases and 8 HME cases indicated that the duplex real-time PCR assay was more sensitive than the nested PCR assay. The infection of Citellus undulatus Pallas with A. phagocytophilum and E. chaffeensis was first confirmed in Xinjiang Province and the positive rate was 3.1% for A. phagocytophilum, 6.3% for E. chaffeensis and 3.1% for co-infection with both pathogens. The rates of A. phagocytophilum and E. chaffeensis infection of D. silvarum ticks collected from Shanxi Province were 8.2% and 14.8%, respectively, and the co-infection rate was 3.3%. The rates of A. phagocytophilum and E. chaffeensis infection in H. longicornis ticks collected from Shandong Province were 1.6% and 6.3%, respectively, and the co-infection rate was 1.6%.
The Analysis of Antoinette’s Tragic Fate in Wide Sargasso Sea  [PDF]
Lijuan Chen
Open Journal of Social Sciences (JSS) , 2014, DOI: 10.4236/jss.2014.212004
Abstract: Wide Sargasso Sea is the masterpiece of British woman writer Jean Rhys. This novel is regarded as prequel of Jane Eyre. Antoinette is the heroine of the novel, who has a tragic fate which arises great sympathy among readers. The aim of this paper is to explore the causes that lead to her death, through analyzing the social context of her life, her growing family environment as well as her personal life path.
Inequalities for Dual Orlicz Mixed Quermassintegrals  [PDF]
Lijuan Liu
Advances in Pure Mathematics (APM) , 2016, DOI: 10.4236/apm.2016.612067
Abstract:

In this paper, we establish the dual Orlicz-Minkowski inequality and the dual Orlicz-Brunn-Minkowski inequality for dual Orlicz mixed quermassintegrals.

Purification and characterization of alpha-amino acid ester hydrolase from Xanthomonas rubrillineans
红纹黄单胞菌α-氨基酸酯水解酶的分离纯化及酶学性质

Feng Qu,Baxian Yi,Lijuan Ye,
屈凤
,易八贤,叶丽娟

微生物学报 , 2012,
Abstract: Objective] The intracellular α-amino acid ester hydrolase(AEH) from Xanthomonas rubrillineans was purified and characterized.Methods] AEH was extracted by butyl acetate,and then purified to electrophoretic homogeneity by calcium phosphate gel precipitation,ammonium sulfate fraction precipitation,anion exchange with DEAE Sephadex A-50,cation exchange with CM cellulose 52,and Sephadex G 200 column chromatography.Results] The subunit molecular mass of AEH was 70 kDa by SDS-PAGE.The optimal reaction pH for cefaclor synthesis was 6.8,and optimal temperature was 42 ℃.The enzyme was stable between pH 5.0 and 8.0,and at 35 ℃.The enzyme activity was enhanced by Mn2+ and Ca2+,however was strongly inhabited by Cu2+,Fe2+ and high concentration of acetone.The kinetic parameters that the enzyme hydrolyzed D-Phenylglycine methyl ester,D-Hydroxyphenylglycine methyl ester and cefaclor were determined,and the values of kcat/Km were 123.7±3.7,2.9±0.6 and 101.3±2.1 mmol-1·s-1·L respectively.The kcat/Km values indicated that the enzyme hydrolyzed D-Phenylglycine methyl ester more efficiently than other substrates.The mechanism of enzymatic reaction with bi-substrates by AEH is Ping-Pong kinetics,and the kcat value that the enzyme catalyzed the synthesis of cefaclor is 547.3±38.2 s-1.Conclusion] The studies of AEH from Xanthomonas rubrillineans were rare,and our research may provide an important basis for industrial application of AEH for beta-lactam antibiotics synthesis.
Impact of Gender and Working Environment on the Level of State Anxiety among Primary and Middle school Teachers in China  [PDF]
Lijuan Wang, Dajun Zhang
Journal of Behavioral and Brain Science (JBBS) , 2012, DOI: 10.4236/jbbs.2012.21012
Abstract: State anxiety has become serious among primary and middle school teachers in China. By using stratified random sampling, we selected 2790 primary and middle school teachers as participants. They were from seven regions of China, among them 2278 were valid subjects. Constructed on the basis of anxiety content, the Teachers’ State Anxiety Questionnaire (TSAQ) was used in the investigation. The result of the survey shows that primary and middle school teachers are generally affected by state anxiety; further analysis indicates that the prevalence of state anxiety is relatively higher among male teachers, teachers from key middle schools, teachers working more than 10 hours a day, and teachers loaded with additional task as head teachers. This is directly connected with China’s national situation, teaching envi-ronment, discrepancies in teachers themselves, and their own ability of self-regulation.
Evaluation of Microblog Users’ Influence Based on PageRank and Users Behavior Analysis  [PDF]
Lijuan Huang, Yeming Xiong
Advances in Internet of Things (AIT) , 2013, DOI: 10.4236/ait.2013.32005
Abstract: This paper explores the uses’ influences on microblog. At first, according to the social network theory, we present an analysis of information transmitting network structure based on the relationship of following and followed phenomenon of microblog users. Informed by the microblog user behavior analysis, the paper also addresses a model for calculating weights of users’ influence. It proposes a U-R model, using which we can evaluate users’ influence based on PageRank algorithms and analyzes user behaviors. In the U-R model, the effect of user behaviors is explored and PageRank is applied to evaluate the importance and the influence of every user in a microblog network by repeatedly iterating their own U-R value. The users’ influences in a microblog network can be ranked by the U-R value. Finally, the validity of U-R model is proved with a real-life numerical example.

Characteristics of anxiety among primary and middle school teachers: a Content-based approach to state anxiety  [PDF]
Lijuan Wang, Dajun Zhang
Health (Health) , 2012, DOI: 10.4236/health.2012.41006
Abstract: Anxiety has become one of the major psychological problems which harass people in modern times; this problem is particularly obvious with primary and middle school teachers. Based on the contents of anxiety, the self-compiled Teachers’ State Anxiety Questionnaire (TSAQ) was used to investigate the effective of 1930 subjects in seven regions of China through stratified random sampling methodology. The results show that that the sense of anxiety for satisfaction and control is higher in male teachers; and the sense of teachers’ anxiety for satisfaction and conflict is severe in the key middle school; and the sense of conflict anxiety in the married teachers is the highest; the anxiety in all are high in teachers with poor physical health and main teacher as well as the teachers with poor economic situation in their family. The feasibility of measures to improve the teachers’ state anxiety in primary and middle school should be considered to enhance the level of teachers’ mental health, work efficiency and quality of life.
A NEW METHOD FOR MAPPING HIGHLAND LAKE ISOBATH
高原湖泊等深线测绘新方法研究

Jia Lijuan,Ning Ping,Qu Guangfei,and Deng Wenwu,
贾丽娟
,宁 平,瞿广飞,邓文武

大地测量与地球动力学 , 2012,
Abstract: Highland lakes have complex terrain features.During engineering survey and research trials,due to natural conditions,capital and human resource constraints,when a fixed-point sampling is needed on the lake surface,it is difficult with the conventional measurement methods to achieve accurate depth and the horizontal distance.Taking the mapping process of 8 m isobath near Yangzonghai lake gushing point as an example,a new simple method is suitable to the lake isobath and surface horizontal distance measurement is put forward and analyzed.The new method has great reference value for non-mapping professionals to map lake isobath lines and lake surface horizontal distance.
The Innovation and Perfection of Chinese Enterprise Form  [PDF]
Nannan Qu
Open Journal of Social Sciences (JSS) , 2015, DOI: 10.4236/jss.2015.38007
Abstract: China’s enterprise form adopts legal principle, lack of flexibility and innovation, which restricts the development of China’s economy. The latest revision of the company law has abolished the restriction of registered capital and loosened of market access, and has brought the opportunity and challenge to the innovation and perfection of enterprise form. This paper mainly discusses the value of the guarantee of Limited Liability Company based on the foreign experience to innovate and perfect our country’s enterprise form, and to better promote the development of the socialist market economy.
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