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Search Results: 1 - 10 of 10365 matches for " Liangkun Long "
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Engineering the Expression and Characterization of Two Novel Laccase Isoenzymes from Coprinus comatus in Pichia pastoris by Fusing an Additional Ten Amino Acids Tag at N-Terminus
Chunjuan Gu, Fei Zheng, Liangkun Long, Jing Wang, Shaojun Ding
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0093912
Abstract: The detail understanding of physiological/biochemical characteristics of individual laccase isoenzymes in fungi is necessary for fundamental and application purposes, but our knowledge is still limited for most of fungi due to difficult to express laccases heterologously. In this study, two novel laccase genes, named lac3 and lac4, encoding proteins of 547 and 532-amino acids preceded by 28 and 16-residue signal peptides, respectively, were cloned from the edible basidiomycete Coprinus comatus. They showed 70% identity but much lower homology with other fungal laccases at protein level (less than 58%). Two novel laccase isoenzymes were successfully expressed in Pichia pastoris by fusing an additional 10 amino acids (Thr-Pro-Phe-Pro-Pro-Phe-Asn-Thr-Asn-Ser?)tag at N-terminus, and the volumetric activities could be dramatically enhanced from undetectable level to 689 and 1465 IU/l for Lac3 and Lac4, respectively. Both laccases possessed the lowest Km and highest kcat/Km value towards syringaldazine, followed by ABTS, guaiacol and 2,6-dimethylphenol similar as the low redox potential laccases from other microorganisms. Lac3 and Lac4 showed resistant to SDS, and retained 31.86% and 43.08% activity in the presence of 100 mM SDS, respectively. Lac3 exhibited higher decolorization efficiency than Lac4 for eleven out of thirteen different dyes, which may attribute to the relatively higher catalytic efficiency of Lac3 than Lac4 (in terms of kcat/Km) towards syringaldazine and ABTS. The mild synergistic decolorization by two laccases was observed for triphenylmethane dyes but not for anthraquinone and azo dyes.
Analysis of bacterial colonization associated with Gigaspora margarita spores by green fluorescence protein (GFP) marked technology

Liangkun Long,Qing Yao,Yuncan Ai,Honghui Zhu,

微生物学报 , 2009,
Abstract: Abstract: Objective] We analyzed bacterial colonization associated with spores of arbuscular mycorrhizal fungi (AMF) Gigaspora margarita, to indicate their ecological niche, and to provide information for further researches on their populations or functions. Methods] Six bacteria strains (Peanibacillus sp. M060106-1, Peanibacillus sp. M061122-2, Peanibacillus sp. M061122-6, Bacillus sp. M061122-4, Bacillus sp. M061122-10 and Brevibacillus sp. M061122-12) isolated from G. margarita spores were tagged with green fluorescence protein (GFP) using the carrier plasmid pNF8 (gfp-mut1). We analyzed the ecological niche and population dynamics of tagged strains on G. margarita under different conditions by using fluorescent microscope and/or plate counts. Results] Four strains (M060106-1, M061122-6, M061122-10 and M061122-12) were tagged with GFP, showing high plasmid stability. These tagged strains possessed the basic characteristics identical to their original strains and, hence, were fit for short-term study of environmental colonization. All four GFP-tagged strains colonized the spore wall of G. margarita, and M061122-6 and M061122-12 further colonized the fungal hyphae. Under different pH conditions, the population dynamic of each GFP-tagged strain on the spores showed the same trend, i.e. first increased and then decreased, and the effects on the population size varied with different pH value. GFP-tagged strains colonized the spores of low viability more easily than those of high viability, and the population dynamic on the spores of high viability was different for each tagged strain. Conclusion] The isolated bacteria associated with G. margarita spores can re-colonize the fungal spores, whereas their colonizing ability depends on their characteristics and environmental factors. These data contributes to the further understanding of populations and functions of AMF-associated bacteria.
Association of Six Single Nucleotide Polymorphisms with Gestational Diabetes Mellitus in a Chinese Population
Ying Wang, Min Nie, Wei Li, Fan Ping, Yingying Hu, Liangkun Ma, Jinsong Gao, Juntao Liu
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0026953
Abstract: Background To investigate whether the candidate genes that confer susceptibility to type 2 diabetes mellitus are also correlated with gestational diabetes mellitus (GDM) in pregnant Chinese women. Methodology/Principal Findings In this study, 1764 unrelated pregnant women were recruited, of which 725 women had GDM and 1039 served as controls. Six single nucleotide polymorphisms (rs7754840 in CDKAL1, rs391300 in SRR, rs2383208 in CDKN2A/2B, rs4402960 in IGF2BP2, rs10830963 in MTNR1B, rs4607517 in GCK) were genotyped using TaqMan allelic discrimination assays. The genotype and allele distributions of each SNP between the GDM cases and controls and the combined effects of alleles for the risk of developing GDM were analyzed. We found that the rs4402960, rs2383208 and rs391300 were statistically associated with GDM (OR = 1.207, 95%CI = 1.029–1.417, p = 0.021; OR = 1.242, 95%CI = 1.077–1.432, p = 0.003; OR = 1.202, 95%CI = 1.020–1.416, P = 0.028, respectively). In addition, the effect was greater under a recessive model in rs391300 (OR = 1.820, 95%CI = 1.226–2.701, p = 0.003). Meanwhile, the joint effect of these three loci indicated an additive effect of multiple alleles on the risk of developing GDM with an OR of 1.196 per allele (p = 1.08×10?4). We also found that the risk alleles of rs2383208 (b = ?0.085, p = 0.003), rs4402960 (b = ?0.057, p = 0.046) and rs10830963 (b = ?0.096, p = 0.001) were associated with HOMA-B, while rs7754840 was associated with decrease in insulin AUC during a 100 g OGTT given at the time of GDM diagnosis (b = ?0.080, p = 0.007). Conclusions/Significance Several risk alleles of type 2 diabetes were associated with GDM in pregnant Chinese women. The effects of these SNPs on GDM might be through the impairment of beta cell function and these risk loci contributed additively to the disease.

Li Zhangming,Yang Liangkun,Wang Jingtao,

岩石力学与工程学报 , 2004,
Abstract: 在不同应变速率与不同侧限压力下晶硝凝灰岩强度及变形试验基础上,基于莫尔-库仑强度理论,建立了考虑应变率与侧限压力效应的二次局部破坏强度准则,讨论了模型参数意义及确定方法。该强度模型表明介质破坏是一个过程,反映了强度突变的应变率阈值效应与侧限压力下主破裂面上矿物颗粒团局部二次破坏的力学机制。
Letter to the editor  [PDF]
Long Jin
Forensic Medicine and Anatomy Research (FMAR) , 2014, DOI: 10.4236/fmar.2014.21001
Abstract: Forensic Medicine and Anatomic Research
Discussion and Analysis on Public-Private-Partnership Industry Fund Mode under New Urbanization Background  [PDF]
Fang Long
Open Journal of Social Sciences (JSS) , 2016, DOI: 10.4236/jss.2016.42012
Abstract: There is larger financing gap in the public facility construction during the promotion of new urbanization in China. It may relieve the difficulty in Public-Private-Partnership project financing through introducing Public-Private-Partnership industry fund mode, flexibly and effectively lead the social capital to engage in the infrastructure construction, and improve the public service efficiency. Based on the feasibility analysis of Public-Private-Partnership industry fund mode, this article has combed the operation ways of Public-Private-Partnership industry fund mode, and proposed the countermeasures for promoting Public-Private-Partnership industry fund mode on account of existing problems on such basis.
Analysis of Total Factor Productivity in China’s Commercial Banks
—Based on Hicks-Moorsteen TFP Indicators

Long Yi
Open Journal of Social Sciences (JSS) , 2016, DOI: 10.4236/jss.2016.44018
Abstract: The paper uses Hicks-Moorsteen TFP indicator to measure the changes of total factor productivity in China’s commercial banks from 2010 to 2014 on the basis of big data in BankScope database. By analyzing the sample data and total factor productivity, the paper finds out in-depth motives of the dynamic changes of TFP. At present, domestic commercial banks are at the stage of deepening reforms. Thus, exploring into the motives that drive the efficiency of commercial banks in recent years and affecting factors is of great instructive roles for national supervision institutions’ formulating relevant monitoring policies, improving banks’ efficiency in resource allocation, and launching profound reforms in banks.
Study on Party Construction for Students in Southwest Petroleum University  [PDF]
Long Guo
Creative Education (CE) , 2016, DOI: 10.4236/ce.2016.74073
Abstract: To strengthen and improve college students’ party construction in the new period is very important, and it has a great significance. The university is the cradle of cultivating a new generation of the motherland, carrying the hope of the future. Strengthening the university student party construction work, and training more excellent party members have very important role in realizing the great rejuvenation of the Chinese nation. The university student party building work has made some achievements in recent years, but it still exists some problems which cannot be ignored. This paper makes a deep study on the new situation and new problems about the university student party building work, and finds out some measures and countermeasures of some new methods for university students to provide the theoretical support for the work of Party building.
Research of Confucianism Education Method in Chinese College Students’ Ideological and Political Education  [PDF]
Long Guo
Creative Education (CE) , 2016, DOI: 10.4236/ce.2016.77109
Abstract: With the rapid development of Chinese economy and society, the ideological condition of college students continuously presents new characteristics, and also brings challenge to ideological and political education work effectiveness. Because of the upsurge of “Chinese culture” in recent years, some scholars have begun to study the idea of Confucian psychological education. How to integrate Confucian psychological education into the ideological and political education of college students will become a new hot spot and the difficulty. Study of Confucian thoughts on psychological education, using Confucian thoughts on psychological education and methods to strengthen college students’ psychological quality, to strengthen the cultivation of students’ psychological quality, and to further enhance the system and effectiveness of the ideological and political education of college students, is not only the requirement of students’ healthy growth, and social expectations of university ideological and political education work.
Comparative Study on the Fortune Global 500—Basing on the 2005-2015 Ranking  [PDF]
Xiaojiao Long
Open Journal of Business and Management (OJBM) , 2016, DOI: 10.4236/ojbm.2016.44074
Abstract: Based on the analysis of the world’s top 500 companies list from 2005 to 2015, comparative research was conducted between six countries including the United States, China, Japan, Britain, France and Germany, which were representative in both revenue and profit among the Fortune Global 500. This paper analyzed the quantity, revenue, profit, margin profit and industrial distribution of these corporations from above-mentioned countries. The existing problems of Chinese enterprises were identified, and corresponding suggestions were given.
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