oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2020 ( 7 )

2019 ( 54 )

2018 ( 86 )

2017 ( 83 )

Custom range...

Search Results: 1 - 10 of 42296 matches for " Letícia Martins;Costa "
All listed articles are free for downloading (OA Articles)
Page 1 /42296
Display every page Item
Criopreserva o de ovócitos de bovinos imaturos desnudados ou n o, utilizando o etilenoglicol pelo método da vitrifica o
Costa Eduardo Paulino da,Guimar?es José Domingos,Torres Ciro Alexandre Alves,Fagundes Letícia Martins
Revista Brasileira de Zootecnia , 2002,
Abstract: Objetivou-se avaliar os efeitos da vitrifica o em ovócitos de bovinos após o cultivo in vitro, utilizando o etilenoglicol como crioprotetor. Ovócitos obtidos de ovários de vacas abatidas em matadouro foram distribuídos aleatoriamente em três tratamentos. Tratamento 0 (testemunha): ovócitos n o-desnudados e n o-congelados. Tratamento 1: vitrifica o de ovócitos imaturos n o desnudados, desidratados previamente por cinco minutos em três solu es contendo 20, 20 e 40% de etilenoglicol, acrescidas de 0,3 mol L-1 de trehalose e 20% de PVP, em meio de Talp Hepes. Tratamento 2: vitrifica o de ovócitos imaturos desnudados, conforme o Tratamento 1. Após o descongelamento (imers o em banho-maria a 30oC por 20 segundos), os ovócitos foram reidratados gradativamente, mantendo-os por 6 minutos em cada uma das solu es a seguir, sucessivamente: meio Talp Hepes com 20% de etilenoglicol + 0,3 mol L-1 de trehalose + 10% de PVP e meio Talp Hepes sem etilenoglicol, trehalose e PVP, onde foram lavados três vezes. Posteriormente, os ovócitos foram cultivados a 38,5oC, com 95% de umidade e atmosfera de 5% de CO2 por 24 horas. Após o cultivo, os ovócitos foram fecundados e os embri es cultivados in vitro por sete dias. Foi encontrada uma taxa de matura o nuclear de 81 (68/84), 19 (7/36) e 0% (0/31), nos Tratamentos 0, 1 e 2, respectivamente. As taxas de clivagem e de desenvolvimento embrionário foram de 56,4 (102/181) e 54,9% (56/102), 1,7 (1/60) e 0,0% (1/60), 0,0 (0/71) e 0,0% (0/71), nos Tratamentos 0, 1 e 2, respectivamente. Esses resultados indicam que o procedimento de vitrifica o, segundo os protocolos utilizados, n o é indicado para a criopreserva o de ovócitos de bovinos.
Criopreserva??o de ovócitos de bovinos imaturos desnudados ou n?o, utilizando o etilenoglicol pelo método da vitrifica??o
Costa, Eduardo Paulino da;Guimar?es, José Domingos;Torres, Ciro Alexandre Alves;Fagundes, Letícia Martins;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2002, DOI: 10.1590/S1516-35982002000500008
Abstract: the objective was to evaluate the effects of vitrification of immature bovine oocytes after in vitro culture, by the use of cryoprotectors ethylene glycol. oocytes from cows ovaries from slaughters houses were randomly alocated into three treatments. treatment 0 (control): frozen-thawed undesnude oocytes; treatment 1, immature vitrificated undesnude oocytes dehydrated for 5 minutes in each of the following solutions of 20, 20 and 40% of ethylene glycol, respectively, associated to 0.3 mol l-1 of trehalose and 20% of pvp, in media talp hepes, and, treatment 2, the same as treatment 1, but desnudes oocytes. after frozen-thawed of the oocytes (imersion in water bath at 30oc for 20 seconds), the oocytes were gradually rehydrated, in the following sequence of solutions: media talp hepes with 20% of ethylene glycol + 0.3 mol l-1 of trehalose + 10% of pvp and media talp hepes without ethylene glycol, trehalose and pvp, were washed three times. ultimately, the oocytes were cultured at 38.5oc, with 95% umidity and atmosphere of 5% of co2 for 24 hours. after culture, the oocytes were fertilized and the embryos cultured in vitro for seven days. the nuclear maturation were 81 (68/84), 19 (7/36) and 0% (0/31), for treatments 0, 1 and 2, respectively. the cleavage and development rates were: 56.4(102/181) and 54,9% (56/102), 1,7. (1/60) and 0,0% (1/60), 0,0 (0/71) and 0,0% (0/71), for the treatments 1, 2 e 3, respectively. these results show that the vitrification procedures, by the used protocols, are not indicated for bovine oocytes cryopreservation.
Vitrifica??o de ovócitos desnudados ou n?o e previamente maturados in vitro
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Silva, Trícia Osório da;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000500004
Abstract: this study aimed at the evaluation of the effects from cryopreservation of bovine oocytes in vitro matured, by using ethylene glycol (eg) associated to trehalose and polyvinylpyrrolidone (pvp), of ovary oocytes of slaughtered cows, randomly assigned to three treatments. treatment 0 (t0 - control): oocytes that were desnuded and not vitrified. treatment 1 (t1): cryopreservation of in vitro matured oocytes with cumulus oophorus. tratamento 2 (t2): cryopreservation of in vitro matured desnuded oocytes. the percentage of recovered oocytes after cryopreservation and with normal morphology was different for vitrified oocytes (94.7 and 76.8%; 69.5 and 48.85% for t1 and t2, respectively). the main changes ultrastructural in vitrificated oocytes were prematurely released of cortical granules. later, all normal oocytes were fecundated and cultivated at 38.5oc in atmosphere with 5% co2 for seven days. the fecundation and cleavage rates for treatments were different (56.2, 41.7 and 12.5%; 36.3, 0.0 and 0.0%, for t0, t1 and t2, respectively). morulas and blastocysts were obtained only in t0 (34.5%). these results indicate that, the used protocols, for vitrification procedure is not indicated for cryopreservation of matured bovine oocytes.
In vitro sensitivity of dermatophytes to urea
Martins, José Eduardo Costa;Corim, Simone Miwa;Arriagada, Giovanna Letícia Hernández;Melo, Natalina Takahashi de;Heins, Elisabeth Maria;
Clinics , 2006, DOI: 10.1590/S1807-59322006000100003
Abstract: objective: urea is commonly used as a keratolytic substance in the treatment of onychomycoses to improve the penetration of antifungal drugs in the lesion sites. the aim of the present study was to investigate the inhibitory action of urea on samples of dermatophytes in vitro. method: minimum inhibitory concentration of urea was determined for 31 samples of dermatophytes cultured in sabouraud-dextrose broth containing different concentrations (7.5% up to 40%) of urea. absence of growth was the criterion adopted to determine the minimum inhibitory concentration. results: the majority of samples (87%) were sensitive to urea at 12.5%, or less. 2 isolates of trichophyton tonsurans and 2 of trichophyton rubrum required 30%, and 40% urea, respectively, to be completely inhibited. conclusion: in vitro results demonstrate inhibitory activity of urea on dermatophytes, suggesting that it could be used as an adjuvant in topical treatments.
In vitro sensitivity of dermatophytes to urea
Martins José Eduardo Costa,Corim Simone Miwa,Arriagada Giovanna Letícia Hernández,Melo Natalina Takahashi de
Clinics , 2006,
Abstract: OBJECTIVE: Urea is commonly used as a keratolytic substance in the treatment of onychomycoses to improve the penetration of antifungal drugs in the lesion sites. The aim of the present study was to investigate the inhibitory action of urea on samples of dermatophytes in vitro. METHOD: Minimum inhibitory concentration of urea was determined for 31 samples of dermatophytes cultured in Sabouraud-dextrose broth containing different concentrations (7.5% up to 40%) of urea. Absence of growth was the criterion adopted to determine the minimum inhibitory concentration. RESULTS: The majority of samples (87%) were sensitive to urea at 12.5%, or less. 2 isolates of Trichophyton tonsurans and 2 of Trichophyton rubrum required 30%, and 40% urea, respectively, to be completely inhibited. CONCLUSION: In vitro results demonstrate inhibitory activity of urea on dermatophytes, suggesting that it could be used as an adjuvant in topical treatments.
Regiospecific synthesis of new non-condensed heteropolycyclic systems from beta-heteroaryl-beta-methoxyvinyl trihalomethyl ketones
Bonacorso, Helio G.;Oliveira, Marlí R.;Costa, Michelle B.;Silva, Letícia B. da;Zanatta, Nilo;Martins, Marcos A. P.;Flores, Alex F. C.;
Journal of the Brazilian Chemical Society , 2005, DOI: 10.1590/S0103-50532005000500029
Abstract: a novel series of twelve heteroaroyl-2-pyrazolines trihalomethyl and substituted heteroaryl, as non-condensed heteropolycyclic systems, have been synthesized in one-step in 50-78% yield from the regiospecific cyclocondensation reaction of 1,1,1-trifluoro(chloro)-4-methoxy-4-(2-furyl)- and 4-(2-thienyl)-3-buten-2-ones (b-alkoxyvinyl trihalometil ketones) with furoic hydrazide, 2-thiophenecarboxylic hydrazide and isonicotinic acid hydrazide (heteroaroylhydrazines) under mild conditions in methanol as solvent.
Efeitos da estimula??o temporária atrial direita na preven??o da fibrila??o atrial no pós-operatório de revasculariza??o do miocárdio com circula??o extracorpórea
Avila Neto, Vicente;Costa, Roberto;Silva, Kátia Regina;Martins, André Luiz Mendes;Moreira, Luiz Felipe Pinho;Santos, Letícia Bezerra;Melo, Ricardo F. de Azevedo;
Revista Brasileira de Cirurgia Cardiovascular , 2007, DOI: 10.1590/S0102-76382007000300010
Abstract: objective: to evaluated the effects of temporary atrial pacing to prevent the atrial fibrillation after coronary artery bypass graft surgery and the risk factors to the occurrence of this arrhytmia. methods: we have studied 160 patients who, at the end of coronary artery bypass graft surgery, were submitted to epicardial electrode implantation in the right atrium lateral wall. they were randomized into two groups: non-pacing (np) group and right atrial (ra) pacing group. the cardiac rhythm was monitorized over 72 hours following to the end of surgery and the variables studied were as follow: incidence of atrial fibrillation; the risk factors pre-, intra-, and postoperative for its occurrence, and postoperative events. results: there were 21 (13.1%) episodes of atrial fibrillation, 20 in np group and one in ra group. the relative risk (rr) for development of atrial fibrillation was 0.18 (95% ci; 0.05-0.60) for the ra group when compared to the np group. the logistic regression identified that the study variables, such as younger age; use of beta-blockers in the preoperative, and the presence of right atrial pacing had been associated to a lower odds ratios (ors) for the occurrence of atrial fibrillation in the postoperative. conclusions: the temporary atrial pacing reduced the incidence of atrial fibrillation after the cabg surgery. older age and a non-atrial pacing were the independent predictive factors of the occurrence of this arrhythmia.
Congela??o de ovócitos desnudados ou n?o, maturos e imaturos de bovinos, utilizando o Etileno Glicol pelo método convencional
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Guimar?es, José Domingos;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000800014
Abstract: the objective of this study was to evaluate the cryopreservation of in vitro mature or immature oocytes by conventional method. the experiment was conducted using oocytes of cows' ovaries from slaughterhouse, distributed into six treatments: unfrozen oocytes with the cumulus oophorus cells (t1) and naked (t2) which were submitted to the process of miv, fiv and civ; immature oocytes, with the cumulus oophorus cells (t3) and naked (t4), which were submitted to the cryopreservation, unfrozen and the miv, fiv and civ; oocytes with the cumulus oophorus cells (t5) and naked (t6), which were submitted to in vitro maturation, cryopreservation, and fiv and civ. the oocytes were frozen by the conventional methods, dehydrated by the immersion in three solutions with 0.6; 1.2 and 1.8 mol l-1 of ethylene glycol (eg) during 5 minutes each phase. the thawing phase was done by the immersion in water-bath at 30 oc during 20 seconds, and so, the oocytes were re-hydrated in three phases (0.9 mol l-1 eg + 0.3 mol l-1 of sacarose; 0.3 l-1 of sacarose without eg and sacarose) for six minutes each one. the mainly ultrastructural changes in cryopreserved matured oocytes were prematurely released of cortical granules. however, the frozen immature and mature oocytes showed vacuolization and disappearance of cristae mitochondrial. the frozen immature oocytes showed the maturation rate of 82.5, 75.4, 9.2 and 5.8% for t1, t2, t3 and t4, respectively. the fecundation rate were 56.2, 0.0, 38.7, 8.6, 63.6 and 16.7% and from the cleavage were 36.3, 7.9, 0.4, 0.0, 0.0 and 0.0% for t1, t2, t3, t4, t5 and t6, respectively. morula and blastocyst were observed only for unfrozen and naked oocytes (t1) (34.5%). these results showed that the frozen protocols affect the viability of the oocytes.
Tolerancia à salinidade avaliada em genótipos de arroz cultivados in vitro
Benitez, Letícia Carvalho;Peters, José Antonio;Bacarin, Marcos Antonio;Kopp, Maurício Marini;Oliveira, Antonio Costa de;Magalh?es Junior, Ariano Martins de;Braga, Eugenia Jacira Bolacel;
Revista Ceres , 2010, DOI: 10.1590/S0034-737X2010000300007
Abstract: plants under natural conditions are exposed to several environmental stresses which affect their metabolism. among them, soil and irrigation water salinity is one of the most serious problems to irrigated agriculture. the aim of this work was to identify through morphologic characters, the genetic variability of 10 genotypes of rice cultivated in vitro, and classify these genotypes regarding salinity tolerance. the treatments were constituted by ten genotypes and four nacl concentrations (0, 4, 8 and 12 mgl-1) added to ms culture medium. after 21 days, several morphological characters were evaluated, to which percentage calculations of relative performance (increase or reduction) were made, considering the absolute value of the control treatment (0mg l-1). all the measured characters had their development reduced in saline substrate. the most sensitive characters to nacl concentration were mean biomass from the aerial parts and from the radicular system. differences were observed among the genotypes studied regarding salinity tolerance. three distinct groups were formed by the upgma hierarchical method and two groups by tocher method, with brs bojuru genotype the most tolerant and brs"7" taim and brs ligeirinho the most sensitive to salinity.
Onicomicose: estudo clínico, epidemiológico e micológico no município de S?o José do Rio Preto
Martins, Edna Alves;Guerrer, Letícia Vieira;Cunha, Keith Cássia;Soares, Márcia Maria Costa Nunes;Almeida, Margarete Teresa Gottardo de;
Revista da Sociedade Brasileira de Medicina Tropical , 2007, DOI: 10.1590/S0037-86822007000500022
Abstract: this was a clinical-epidemiological and mycological study on 184 patients at the university hospital: 200 samples, 142 positive samples, 98 yeasts and 68 filamentous fungi. candida parapsilosis (47%) and trichophyton rubrum (38%) were prevalent. they were 100% sensitive to ketoconazole and 99% sensitive to amphotericin b. the highest prevalences were among women (80%), adults (62%) and toes (84%).
Page 1 /42296
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.