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Search Results: 1 - 10 of 9939 matches for " Leonardo Araujo; "
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Viabilidade da realiza??o de espirometria em pré-escolares
Veras, Tiago Neves;Pinto, Leonardo Araujo;
Jornal Brasileiro de Pneumologia , 2011, DOI: 10.1590/S1806-37132011000100011
Abstract: objective: to determine the rate at which satisfactory spirometry results are obtained (spirometry success rate) in preschool children. methods: we analyzed the spirometry results of children < 6 years of age. all tests were conducted between june of 2009 and february of 2010 in the pulmonary function laboratory of the hospital infantil jeser amarante faria, located in the city of joinville, brazil. the spirometry program employed features an animated incentive (soap bubbles). the procedures were performed by a pediatric pulmonologist, in accordance with the reproducibility and acceptability criteria recommended by the american thoracic society. we attempted to achieve an expiratory time of at least 1 s. the following parameters were measured: fvc, fev0.5, fev1, and the fev1/fvc ratio. results: our sample comprised 74 children. the spirometry success rate was 82%. although the performance improved with age, the difference between younger and older children was not significant (p > 0.05). an average of 6.6 attempts/test were needed in order to achieve acceptable, reproducible curves. all 61 successful tests produced satisfactory fev0.5 and fev1 values. by calculating z scores, we found that 21.6% of the children presented with an obstructive pattern. conclusions: in our sample, the spirometry success rate was high, showing that spirometry is a valid method for assessing pulmonary function in preschool children. the high success rate in our sample might be attributable to the use of an incentive and to the fact that the tests were performed by professionals specializing in pediatrics.
Genetic associations with asthma and virus-induced wheezing: a systematic review
Pinto, Leonardo Araujo;Stein, Renato Tetelbom;Ribeiro, José Dirceu;
Jornal Brasileiro de Pneumologia , 2009, DOI: 10.1590/S1806-37132009001200010
Abstract: various wheezing phenotypes can be identified based on differences in natural histories, risk factors and responses to treatment. in epidemiologic studies, atopic asthma or virus-induced wheezing can be discriminated by the presence or the absence of sensitization to allergens. children with asthma have been shown to present lower levels of lung function. patients with viral respiratory infections evolve from normal lung function to enhanced airway reactivity. the objective of this study was to identify genes and polymorphisms associated with different wheezing phenotypes. using data obtained from the genetic association database, we systematically reviewed studies on genes and polymorphisms that have been associated with virus-induced wheezing or atopic asthma. the research was carried out in february of 2009. genes associated with the studied outcomes in more than three studies were included in the analysis. we found that different genes and loci have been associated with virus-induced wheezing or atopic asthma. virus-induced wheezing has frequently been associated with il-8 polymorphisms, whereas atopic asthma and atopy have frequently been associated with th2 cytokine gene (cd14 and il-13) polymorphisms on chromosome 5. this review provides evidence that different wheezing disorders in childhood can be differently affected by genetic variations, considering their role on airway inflammation and atopy. future studies of genetic associations should consider the different wheezing phenotypes in infancy. in addition, stratified analyses for atopy can be useful for elucidating the mechanisms of the disease.
Anestésicos locais: intera??o com membranas biológicas e com o canal de sódio voltagem-dependente
Araujo, Daniele Ribeiro de;Paula, Eneida de;Fraceto, Leonardo Fernandes;
Química Nova , 2008, DOI: 10.1590/S0100-40422008000700032
Abstract: many theories about the mechanism of action of local anesthetics (la) are described in the literature. two types of theories can be distinguished: those that focus on the direct effects of la on their target protein in the axon membranes, i.e. the voltage-gated sodium channel and the ones that take into account the interaction of anesthetic molecules with the lipid membrane phase for the reversible nerve blockage. since there is a direct correlation between la hydrophobicity and potency, it is crucial to take this physico-chemical property into account to understand the mechanism of action of la, be it on the sodium channel protein, lipid(s), or on the whole membrane phase.
Um referencial teórico -metodológico para o desenvolvimentode pesquisas sobre atitude: a Teoria do Comportamento Planejado de Icek Ajzen
Leonardo Albuquerque Heidemann,Ives Solano Araujo,Eliane Angela Veit
Revista electr?3nica de investigaci?3n en educaci?3n en ciencias , 2012,
Abstract: A variety of research on Science Teaching that involve attitudes assessment have shown poor and/or confused results due to the lack of a consistent theoretical and methodological framework. The discrepancy in the signification given by researchers to the cthe interpretation of the results and the establishment of relationships between the obtained conclusions. In this work, we defend the Theory of Planned Behavior (TPB), by Icek Ajzen, as an useful framework for researches tAccording to TPB, attitudes, jointly with aspects related to the social pressure perceived, available infrastructure and individuals skills are predictors of their behavioral intentions related to behavior. This theory is potentially useful to support researches to create instruments for the measurement of attitudes, and to help them to interpret the results and plan interventions programs that improve their chances to reach the respectives
Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
Aranha, Silvia de Araujo;Albuquerque, Leonardo Cunha de;Boiteux, Leonardo Silva;Inoue-Nagata, Alice Kazuko;
Tropical Plant Pathology , 2011, DOI: 10.1590/S1982-56762011000100002
Abstract: a survey of okra begomoviruses was carried out in central brazil. foliar samples were collected in okra production fields and tested by using begomovirus universal primers. begomovirus infection was confirmed in only one (#5157) out of 196 samples. total dna was subjected to pcr amplification and introduced into okra seedlings by a biolistic method; the bombarded dna sample was infectious to okra plants. the dna-a and dna-b of isolate #5157 were cloned and their nucleotide sequences exhibited typical characteristics of new world bipartite begomoviruses. the dna-a sequence shared 95.6% nucleotide identity with an isolate of sida micrantha mosaic virus from brazil and thus identified as its okra strain. the clones derived from #5157 were infectious to okra, sida santaremnensis and to a group of solanaceae plants when inoculated by biolistics after circularization of the isolated insert, followed by rolling circle amplification.
Aplica??o de IBA para o enraizamento de miniestacas de Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden
Brondani, Gilvano Ebling;Grossi, Fernando;Wendling, Ivar;Dutra, Leonardo Ferreira;Araujo, Marla Alessandra;
Acta Scientiarum. Agronomy , 2010, DOI: 10.4025/actasciagron.v32i4.4879
Abstract: eucalyptus has great importance in the forestry sector and many advances in the area of improvement have been achieved with the advent of biotechnological techniques. however, some promising genotypes still do not have multiplication protocols with cloning techniques, such as minicutting. the study aimed to evaluate iba concentrations for survival, rooting and vegetative vigor of e. benthamii x e. dunnii minicuttings, and determine the maximum technical efficiency dose. ministumps h12, h19 and h20 clones were cultivated in a clonal minigarden under a semi-hydroponic system. for rooting, the minicutting basal portion was plunged in hydro-alcoholic solutions, whose concentrations were: 0; 2,000; 4,000; 6,000 and 8,000 mg l-1 of iba. the experiment was conducted in a completely randomized design, with the factors consisting of three clones and five iba concentrations, with five replications, containing 10 minicuttings per replication. iba positively influenced the minicutting rooting processes, with differentiated behavior between the clones, with 30.32 to 55.45% rooting variation. the positives increments occurred until the highest iba concentration for h12 and h19 clones. however, the range between 4,000 and 6,000 mg l-1 of iba treatments promoted the best rooting results for the h20 clone.
Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii)
Brondani, Gilvano Ebling;Dutra, Leonardo Ferreira;Wendling, Ivar;Grossi, Fernando;Hansel, Fabricio Augusto;Araujo, Marla Alessandra;
Acta Scientiarum. Agronomy , 2011, DOI: 10.4025/actasciagron.v33i4.8317
Abstract: this study was designed to micropropagate e. benthamii x e. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (bap) and naphthaleneacetic acid (naa) for bud proliferation, and the ratio between bap and gibberellic acid (ga3) in two nutrient media for shoot elongation. nodal segments from h12, h19 and h20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1) of chlorine. explants were grown on ?ms medium supplemented with bap (0, 0.25, 0.50, 0.75 and 1.00 mg l-1) and naa (0, 0.025, 0.050, 0.075 and 0.100 mg l-1) for bud production. they were elongated on ms and ?ms media supplemented with bap (0, 0.05 and 0.10 mg l-1) and ga3 (0, 0.1; 0.2 and 0.3 mg l-1). the 0.50 mg l-1 bap and 0.050 mg l-1 naa combination was optimal for bud proliferation for h12 and h20. ga3 concentrations of 0.10 and 0.20 mg l-1 combined with 0.10 mg l-1 bap on ?ms resulted in the longest shoots, for h12 and h20, respectively. regardless of clone, the rooting rate was low, with an average of 12.0% and 14.4% of plants having roots for in vitro and ex vitro conditions, respectively.
'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage
Pasqual, Moacir;Dutra, Leonardo Ferreira;Araujo, Aparecida Gomes de;Figueiredo, Milene Alves de;
Ciência e Agrotecnologia , 2006, DOI: 10.1590/S1413-70542006000500029
Abstract: the aim of this work was to evaluate the effect of 'ponkan' mandarin (c. reticulata) x 'pêra' sweet orange (c. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1oc during 135 days. every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 ml of ms medium (murashige & skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g l-1) and 0.3 mg l-1 ga3 and 1 g l-1 activated charcoal. those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1oc with a 16-h photoperiod and 32 μmol m-2 s-1 light intensity. immature fruits can be stored for posterior excision and embryos culture. fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. it was observed a better development of the aerial part and root system of plantlets from 'ponkan' mandarin x 'pêra' sweet orange embryos in ms medium with 12-18 g l-1 sucrose.
Entropy of a Zipfian Distributed Lexicon
Leonardo Carneiro Araujo,Tha?s Cristófaro-Silva,Hani Camille Yehia
Statistics , 2013,
Abstract: This article presents the calculation of the entropy of a system with Zipfian distribution and shows that a communication system tends to present an exponent value close to one, but still greater than one, so that it might maximize entropy and hold a feasible lexicon with an increasing size. This result is in agreement with what is observed in natural languages and with the balance between the speaker and listener communication efforts. On the other hand, the entropy of the communicating source is very sensitive to the exponent value as well as the length of the observable data, making it a poor parameter to characterize the communication process.
Methods for Breaking Dormancy of Seeds of Tropical Forage Legumes  [PDF]
Leonardo F. de Morais, Jo?o C. C. Almeida, Bruno B. Deminicis, Fábio T. de Pádua, Mirton J. F. Morenz, Jo?o B. R. de Abreu, Raphael P. Araujo, Delci D. de Nepomuceno
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.513196
Abstract:

The aim of this study was to evaluate methods for breaking dormancy of seeds Neonotonia wightii (perennial soybean), Macrotiloma axilare (archer), Pueraria phaseoloides (tropical kudzu), Calopogonium mucunoides (calopo), which were subjected to the following treatments for physical breaks and physiological dormancy: 1) control; 2) scarification with sandpaper; 3) immersion in H2SO4 98% for five minutes and subsequent washing in water; 4) preheating at 60°C for 150 minutes in an air circulating oven; 5) 0.2% KNO3; and 6) gibberellic acid (GA3 0.5%). The results showed that using H2SO4 to break seed dormancy archer and perennial soybean and calopo scarification with sandpaper were the most recommended treatments. Tropical Kudzu presented physiological response to treatments with the use of gibberellic acid and physical treatment using immersion H

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