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Search Results: 1 - 10 of 972 matches for " Leishmania braziliensis "
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Growth curves of Leishmania braziliensis braziliensis Promastigotes and surface antigen expression before and after adaptation to Schneider's drosophila medium as assessed by anti-Leishmania human sera
Celeste, Beatriz J.;Guimar?es, M. Carolina S.;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1988, DOI: 10.1590/S0036-46651988000200001
Abstract: leishmania braziliensis braziliensis(mhom/br/75/m2903) was grown in schneider's drosophila medium. in one set of experiments promastigotes were already adapted to the medium by means of serial passages whereas in the second cells were grown in a biphasic medium and transfered to the liquid. growth was more abundant for culture medium adapted cells; degenerate cells in small numbers as well as dead ones were present from day 5 for promastigotes adapted to liquid medium and from day 3 for newly adapted cells. synthesis of surface antigens differed according to length of cell culture as assessed by the titer of five mucocutaneous leishmaniasis sera on subsequent days. five days of culture for cells already adapted to the culture medium and 3 days for newly adapted ones were judged to be the best for the preparation of immunofluorescence antigens.
Isolation and isoenzyme characterization of Leishmania (Viannia) braziliensis from a case of human cutaneous leishmaniasis in northeast centre of the state of S?o Paulo
Pinto, MC;Rosa, JA da;Fernandes, ZMT;Graminha, MAS;Mine, JC;Allegretti, SM;Delort, S;Riedel, C;Paes, EL;Cupolillo, E;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000700009
Abstract: the diagnosis of human cutaneous leishmaniasis in small towns is sometimes made without the species identification of the leishmania, even in areas without previous epidemiological surveys. here we report the isolation of a leishmania strain from a patient of rinc?o, state of s?o paulo, that was identified by isoenzyme characterization as l. (viannia) braziliensis. sand fly collections were made in the area where the patient live in order to investigate the likely vector species.
Cutaneous Leishmaniasis in Venezuela Caused by Infection with a New Hybrid between Leishmania (Viannia) braziliensis and L. (V.) guyanensis
Delgado Olinda,Cupolillo Elisa,Bonfante-Garrido Rafael,Silva Silvia
Memórias do Instituto Oswaldo Cruz , 1997,
Abstract:
Infec??o natural de Equus asinus por Leishmania braziliensis braziliensis - Bahia, Brasil
Vexenat, Julio A.;Barretto, Air C.;Rosa, Ana de Cassia O.;Sales, Christiane C.;Magalh?es, Albino V.;
Memórias do Instituto Oswaldo Cruz , 1986, DOI: 10.1590/S0074-02761986000200016
Abstract: in corte de pedra, valen?a, state of bahia, a donkey, equus asinus, was found naturally infected with leishmania braziliensis braziliensis. the parasite was isolated from a lesion located on a castration scar, and identified by means of monoclonal antibodies.
Experimental canine mucocutaneous leishmaniasis (Leishmania braziliensis braziliensis)
Pirmez, Claude;Marzochi, Mauro C. A.;Coutinho, Sergio G.;
Memórias do Instituto Oswaldo Cruz , 1988, DOI: 10.1590/S0074-02761988000200001
Abstract: four mongrel dogs were intradermically inoculated with 3 x 10**6 leishmania braziliensis braziliensis promastigotes. three out of the four animals developed cutaneous lesions respectively 4, 7, and 8 months after. the fourth dog did not develop lesion at the inoculation site, but a mucosal ulcer was seen 16 months after the inoculum. clinical, histopathological, and serological findings were similar to what is found in natural canine infection as well as in the human disease. these results suggest that dogs may be an useful model for l. b. braziliensis infection.
Foco de leishmaniose tegumentar americana nos municípios de Viana e Cariacica, Estado do Espirito Santo, Brasil
Barros,Gelcilio Coutinho; Sessa,Paulo Augusto; Mattos,Enilse Antunes de; Carias,Valquiria Rocha Daher; Mayrink,Wilson; Alencar,José Tasso Aires de; Falqueto,Aloisio; Jesus,Antonio Claudino de;
Revista de Saúde Pública , 1985, DOI: 10.1590/S0034-89101985000200005
Abstract: a mucocutaneous leishmaniasis focus occuring in four village situated in viana and cariacica counties, located near the sea-coast of espírito santo state, brazil, is described. seventy one cases of the disease were diagnosed between may 1978 and december 1982. the laboratorial confirmation was obtained by the montenegro test in 66 patients and by biopsy in 15. the 0 to 10 year age-group was the most affected. the strains of the parasites isolated from 3 patients and 27 dogs were classified as leishmania braziliensis braziliensis. among 257 wild animals captured the leishmania was isolated in two proechimys iheringi only, and identified as l. mexicana aristedesi. the most numerous sandfly specimens (lutzomyia intermedia, l. fischeri and l. migonei) were captured with greater frequency both inside and outside residences. the possibility of the transmission's occuring both inside and outside residences was noteworthy.
Efeito leishmanicida in vitro de Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae)
Moreira, Rosilene C.R.;Costa, Graciomar C.;Lopes, Thaiana C.;Bezerra, Jeamile L.;Guerra, Rosane N.M.;Rebêlo, José Manuel M.;Ribeiro, Maria Nilce S.;Nascimento, Flávia R.F.;Costa, Jackson M.L.;
Revista Brasileira de Farmacognosia , 2007, DOI: 10.1590/S0102-695X2007000100013
Abstract: leishmanicidal activity of the hydroalcoholic extract of stachytarpheta cayennensis, species that is usually employed in ulcers caused by leishmania, was evaluated in vitro using leishmania braziliensis and l. amazonensis promastigotes forms. the hydroalcoholic extract was prepared from dried leaves and used in l. amazonensis and l. braziliensis promastigotes cultures at concentrations of 500 to 32.5 μg/ml. after 24 hours the promastigotes forms were quantified and the ic50 was calculated. the cytotoxicity of the extract was evaluated using peritoneal macrophages. the extract presented a dose and specie-dependent leishmanicidal effect to leishmania promastigotes, mainly to the l. braziliensis ones. the cytotoxic effect was not observed in macrophage cultures. in conclusion, the hydroalcoholic extract of s. cayennensis inhibits the growing of leishmania promastigotes forms in vitro accounting for the folk use of this vegetal in skin ulcers caused by leishmania.
Aspectos epidemiológicos da Leishmaniose Tegumentar Americana na regi o Noroeste do Estado do Paraná
M. C.M CURTI,T. G.V SILVEIRA,S. M.A.A. ARRAES,D. A. BERTOLINI
Revista de Ciências Farmacêuticas Básica e Aplicada , 2009,
Abstract: No Estado do Paraná, a primeira notifica o de leishmaniose tegumentar americana ocorreu em 1917 e a partir de 1980 observou-se um aumento do número de casos, mantendo-se endêmica e acometendo pessoas de todas as faixas etárias e em ambos os sexos. Este estudo teve como objetivo realizar um levantamento epidemiológico sobre a ocorrência de LTA em pacientes atendidos no Laboratório de Ensino e Pesquisa em Análises Clínicas da Universidade Estadual de Maringá (LEPAC/UEM). Foi realizado um estudo retrospectivo e descritivo em base de dados secundário de 1986 a 2005, com 1656 pacientes segundo as variáveis: sexo, idade, ocupa o, procedência, local de moradia, forma clínica e diagnóstico. A maioria dos pacientes era do sexo masculino (72,6%) e adquiriu a infec o no Estado do Paraná (97,8%), residia em área urbana (64,3%) dos quais 51,3% adquiriu a infec o durante atividades de lazer. O diagnóstico da maioria dos pacientes foi estabelecido nos dois primeiros meses de evolu o das les es (54,0%) e apresentando a forma cutanea da doen a (88,9%). Uma parcela (34,4%) significativa dos pacientes que residiam em área rural adquiriu a infec o no domicílio ou peridomicílio. O estudo mostra a predominancia da forma cutanea da leishmaniose tegumentar americana e sugere a atividade de lazer e o ambiente do domicilio como fatores preditivos importantes para a infec o. Palavras-Chave: Leishmaniose cutanea. Epidemiologia. Leishmania. Leishmania (Viannia) braziliensis.
Estudo comparativo de técnicas de demonstra??o de amastigotas e isolamento de promastigotas no diagnóstico da leishmaniose tegumentar americana
Sampaio, Raimunda Nonata Ribeiro;Andrade, Gilberto Brown de;Pereira, Antonio César;Silva, Eurico Aparecido da;Cuba, César Augusto Cuba;
Anais Brasileiros de Dermatologia , 2002, DOI: 10.1590/S0365-05962002000500005
Abstract: background: pcr is a highly sensitive procedure for the diagnosis of lta, but it is expensive and not readily available. culture and smearing are practical, but they lack sensitivity. objective: the objective of this work is to compare the latter two methods in order to achieve a higher sensitivity at a lower cost. method: three culture media were compared in the process of isolating leishmaniasis: blood agar difco + schneider + 20% fetal calf serum; difco + schneider + human sterile urine (2%); schneider with sterile human urine (2%). moreover, two techniques were compared for the demonstration of amastigotes: biopsy performed with smears, or matchstick scrapping results: the positivity and contamination indices (29-to-33% and 8-to-11%, respectively, p>0.05) were similar when comparing the cultures. the smears carried out by biopsy or the matchstick scrapping procedure had no significant differences (14 and 19%, respectively, p>0.05). leishmania (viannia) braziliensis predominated. conclusion: in brazil, urine may substitute fetal calf serum. the cost-to-benefit ratio is advantageous. urine has no cost, while the market value of 500 ml of fetal calf serum is $us 185.
Leishmania (Viannia) braziliensis growth in vitro culture relies more on folic acid availability than Leihsmania (Leishmania) amazonensis
Ni?o, Andrea;Camacho, Marcela;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000300017
Abstract: we compared the in vitro growth of promastigotes from two leishmania species in tc-100 and schneider media. leishmania (leishmania) amazonensis replication rates were similar in both tissue culture media and reached maximum rates by 48 h. in contrast leishmania (viannia) braziliensis growth was significantly greater in tc-100 but maximum rates were achieved by 96 h. folic acid appears to be the limiting factor and supplementation of schneider media with this nutrient improved l. (v.) braziliensis replication rates and decreased the time of maximum replication to 48 h.
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