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Search Results: 1 - 10 of 189027 matches for " Leila de Souza;Coimbra Jr "
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Detection of Mycobacterium tuberculosis in sputum from Suruí Indian subjects, Brazilian Amazon
Basta, Paulo Cesar;Oelemann, Maraníbia AC;Oelemann, Walter MR;Fonseca, Leila de Souza;Coimbra Jr, Carlos EA;
Memórias do Instituto Oswaldo Cruz , 2006, DOI: 10.1590/S0074-02762006000600001
Abstract: this investigation aimed at the detection of mycobacterium tuberculosis (mtb) in the sputum of suruí indian subjects from amazonia, brazil. polymerase chain reaction analyses were positive for12 samples, five of which were also culture-positive (n = 147). four mtb genotypes were identified, one of which showed resistance to rifampicin and isoniazid. the study also highlighted one village complex as of particular importance, considering the relatively high number of tuberculosis cases reported and of mtb isolates obtained.
Estrutura etária, natalidade e mortalidade do povo indígena Xavante de Mato Grosso, Amaz?nia, Brasil
Souza,Luciene Guimar?es de; Santos,Ricardo Ventura; Coimbra Jr.,Carlos Everaldo Alvares;
Ciência & Saúde Coletiva , 2010, DOI: 10.1590/S1413-81232010000700058
Abstract: the overall demographic and health characteristics of indigenous peoples in brazil are well-known. they are influenced by factors such as territorial restriction, introduction of diseases, changes in social and economic patterns, reduced access to health services, education, all related to patterns of interaction with the brazilian society. in this paper we analyze demographic and health data on the xavante indigenous people, including comparisons with national data. the data on the xavante, despite limitations related to methodological issues, point to very high mortality levels, way beyond national averages. mortality is particularly high in children below age five. xavante infant mortality is among the highest reported in indigenous peoples in brazil. the results suggest that indigenous peoples are undergoing a complex process of health transition. although infectious diseases persist as leading causes of mortality, deaths associated with chronic non-transmissible diseases and external causes (including violence) are becoming more common among the xavante. the authors emphasize that it is fundamental that more attention should be paid to the improvement of the health and demographic information system on indigenous peoples in brazil.
Sintomas de deficiência nutricional em mudas de Acacia holosericea em resposta à omiss?o de macronutrientes
Sarcinelli, Tathiane Santi;Ribeiro Jr., Emerson Silva;Dias, Luiz Eduardo;Lynch, Leila de Souza;
Revista árvore , 2004, DOI: 10.1590/S0100-67622004000200003
Abstract: acacia holosericea is a leguminous species that has been extensively used in land reclamation programs. the knowledge of its nutritional symptoms allows the identification and correction of deficiencies in individuals planted in degraded substrates. the aim of this work was to characterize the behavior of seedlings of a. holosericea submitted to different nutritional solutions with absence of macronutrients. the treatments consisted of seven nutritional solutions: 1) complete solution (sc); 2) sc - n; 3) sc -p; 4) sc -k; 5) sc -ca; 6) sc -mg; 7) sc -s. treatments -n and -mg were the ones that most affected biomass production. decrease in biomass production occurred in the following order: -n = -mg > -k > -s > -ca > sc > -p. the -n treatment plants formed nodules in their roots, showing higher concentrations of n than those of the sc treatment. except for the sc and -p treatments, all the other treatments showed nutritional deficiency symptoms. the absence of s has led to a change in the natural disposition of new leaves of a. holosericea.
Antimicrobial susceptibility determined by the E test, L?wenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
Freixo, Maria Inês Moura;Caldas, Paulo CS;Said, Abbadi;Martins, Fátima;Brito, Rossana Coimbra;Fonseca, Leila de Souza;Saad, Maria Helena Féres;
Memórias do Instituto Oswaldo Cruz , 2004, DOI: 10.1590/S0074-02762004000100019
Abstract: mycobacterium tuberculosis strains resistant to streptomycin (sm), isoniazid (inh), and/or rifampin (rif) as determined by the conventional l?wenstein-jensen proportion method (ljpm) were compared with the e test, a minimum inhibitory concentration susceptibility method. discrepant isolates were further evaluated by bactec and by dna sequence analyses for mutations in genes most often associated with resistance to these drugs (rpsl, katg, inha, and rpob). preliminary discordant e test results were seen in 75% of isolates resistant to sm and in 11% to inh. discordance improved for these two drugs (63%) for sm and none for inh when isolates were re-tested but worsened for rif (30%). despite good agreement between phenotypic results and sequencing analyses, wild type profiles were detected on resistant strains mainly for sm and inh. it should be aware that susceptible isolates according to molecular methods might contain other mechanisms of resistance. although reproducibility of the ljpm susceptibility method has been established, variable e test results for some m. tuberculosis isolates poses questions regarding its reproducibility particularly the impact of e test performance which may vary among laboratories despite adherence to recommended protocols. further studies must be done to enlarge the evaluated samples and looked possible mutations outside of the hot spot sequenced gene among discrepant strains.
Demography and health of the Xavante Indians of Central Brazil
Souza, Luciene Guimar?es de;Santos, Ricardo Ventura;Pagliaro, Heloisa;Carvalho, Marilia Sá;Flowers, Nancy May;Coimbra Jr., Carlos E. A.;
Cadernos de Saúde Pública , 2011, DOI: 10.1590/S0102-311X2011001000003
Abstract: this study investigates the demographic and health behavior of the xavante indians of mato grosso state, central brazil. data covering the period 1999 to 2004 was collected using information from household censuses and vital statistics. in addition to standard demographic analyses, survival analysis was carried out. results show a young age structure, derived from a combination of high fertility rates (7.7 live births per woman) and declining mortality. mortality rates, especially infant mortality (97 per thousand), remain very high, surpassing regional and national rates. natural increase is the main contributing factor to population growth. the annual population growth rate is 4.4%. results suggest that recent declines in mortality and fertility may be related to transformations in the implementation of basic health care services and internal diversity, the latter of which seems to be associated with local history and sociocultural determinants.
Multiple isolates from Aids patients: aspects of an analysis by a genotypic marker and antimicrobial susceptibilities variations
Saad, Maria Helena Féres;Telles, Maria Alice;Porfirio, Fátima;Ferrazoli, Lucilaine;Fonseca, Leila de Souza;Johnson Jr, Warren;Riley, Lee W;
Memórias do Instituto Oswaldo Cruz , 2000, DOI: 10.1590/S0074-02762000000500021
Abstract: twenty-one mycobacterium avium multisolates, from ten human immunodeficiency virus-infected patients, were typed by restriction fragment length polymorphism using as marker the is1245 and characterized by minimum inhibitory concentration for nine different antibiotics. two out of four patients harboring multisolates with different fingerprint profile, were therefore considered as having a polyclonal infection, since their isolates were taken from sterile site. this result confirms that polyclonal infection caused by m. avium occurs with a nonnegligenciable frequency. analyzing the multisolates susceptibility profile of each patient it was observed that most of them were infected with strains having appreciably different antimicrobial susceptibility patterns, no matter what the genotypic pattern of the strains was. these results have strong implication for the treatment of the patients.
Internalization of components of the host cell plasma membrane during infection by Trypanosoma cruzi
Carvalho, TMU;De Souza, W;Coimbra, ES;
Memórias do Instituto Oswaldo Cruz , 1999, DOI: 10.1590/S0074-02761999000700016
Abstract: epimastigote and trypomastigote forms of trypanosoma cruzi attach to the macrophage surface and are internalized with the formation of a membrane bounded vacuole, known as the parasitophorous vacuole (pv). in order to determine if components of the host cell membrane are internalized during formation of the pv we labeled the macrophage surface with fluorescent probes for proteins, lipids and sialic acid residues and then allowed the labeled cells to interact with the parasites. the interaction process was interrupted after 1 hr at 37oc and the distribution of the probes analyzed by confocal laser scanning microscopy. during attachment of the parasites to the macrophage surface an intense labeling of the attachment regions was observed. subsequently labeling of the membrane lining the parasitophorous vacuole containing epimastigote and trypomastigote forms was seen. labeling was not uniform, with regions of intense and light or no labeling. the results obtained show that host cell membrane lipids, proteins and sialoglycoconjugates contribute to the formation of the membrane lining the pv containing epimastigote and trypomastigote t. cruzi forms. lysosomes of the host cell may participate in the process of pv membrane formation.
Internalization of components of the host cell plasma membrane during infection by Trypanosoma cruzi
Carvalho TMU,De Souza W,Coimbra ES
Memórias do Instituto Oswaldo Cruz , 1999,
Abstract: Epimastigote and trypomastigote forms of Trypanosoma cruzi attach to the macrophage surface and are internalized with the formation of a membrane bounded vacuole, known as the parasitophorous vacuole (PV). In order to determine if components of the host cell membrane are internalized during formation of the PV we labeled the macrophage surface with fluorescent probes for proteins, lipids and sialic acid residues and then allowed the labeled cells to interact with the parasites. The interaction process was interrupted after 1 hr at 37oC and the distribution of the probes analyzed by confocal laser scanning microscopy. During attachment of the parasites to the macrophage surface an intense labeling of the attachment regions was observed. Subsequently labeling of the membrane lining the parasitophorous vacuole containing epimastigote and trypomastigote forms was seen. Labeling was not uniform, with regions of intense and light or no labeling. The results obtained show that host cell membrane lipids, proteins and sialoglycoconjugates contribute to the formation of the membrane lining the PV containing epimastigote and trypomastigote T. cruzi forms. Lysosomes of the host cell may participate in the process of PV membrane formation.
Galls as a Disputed Resource for Female Parasitoid Wasps Contests  [PDF]
Denise Dalbosco Dell’Aglio, Milton de Souza Mendon?a Jr.
Advances in Entomology (AE) , 2015, DOI: 10.4236/ae.2015.33010
Abstract: We investigated how the parasitoid Torymus sp. (Hymenoptera: Torymidae) attacking galls of Schismatodiplosis lantanae (Diptera: Cecidomyiidae) on Lantana camara (Verbenaceae) behaves in the presence of a conspecific female competitor in the patch. Presence of a competitor greatly changed resident exploitation behavior. Wasps alone spent more time in gall exploitation behaviors (walk-antennate and probe) and in post-oviposition behaviors (stationary and groom), and when intruders were present they spent more time walking. The attack strategy was through threatening: raising wings and pointing the antennae towards the opponent. Different from theoretical expectations, residents were not always the attacker. Number of galls in the patch and female wasp size did not affect contest outcomes, although gall exploitation time and time on leaf were significant factors for the probability of having attacks. Overall, the study highlights the fact that intruder interest in the host was the main cause of contests and also this is a unique report both in terms of the target species and the nature of the disputed resource, a gall-inducer inside a singular spatial unit, the gall.
Avalia??o da carga mental de trabalho e do desempenho de medidas de mensura??o: NASA TLX e SWAT
Cardoso, Mariane de Souza;Gontijo, Leila Amaral;
Gest?o & Produ??o , 2012, DOI: 10.1590/S0104-530X2012000400015
Abstract: this study evaluates the mental workload in some activities in an electricity generation company in santa catarina, brazil and compares the mental workload measurements obtained using two commonly used measurement methods- nasa tlx and swat. the mental workload required by both manual and automated assembly of circuit boards was evaluated. the evaluation of the mental workload showed that comparing these two types of activities, the mental requirements appear to be higher during manual assembly tasks. the methods for assessing the mental workload used in studies of ergonomics show workers' skills and limitations, characteristics of work organization, and facilitate the presentation of quantitative and qualitative results. the comparison of the performance of these two mental workload assessment methods proved a relevant investigation to the field of ergonomics since there are few comparative studies on the performance of these methods. with regard to the overall comparison of the performance of these methods, nasa tlx allows the evaluation of mental workload by analyzing several dimensions of the work situation and presents advantages when compared to swat because it can be easily implemented and showed greater acceptance by those who evaluated it
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