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Search Results: 1 - 10 of 15141 matches for " Leandro Casta?eyra-Ruiz "
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Hypertension effects on p73 expression in the rat circumventricular organs and cerebrospinal fluid  [PDF]
Emilia M. Carmona-Calero, Ibrahim González-Marrero, Manuela Castaeyra-Martin, Juan M. González-Toledo, Leandro Castaeyra-Ruiz, Héctor de Paz-Carmona, Agustín Castaeyra-Ruiz, Lidia Ruiz-Mayor, Agustín Castaeyra-Perdomo
World Journal of Neuroscience (WJNS) , 2012, DOI: 10.4236/wjns.2012.22010
Abstract: It has been reported that spontaneously hypertensive rats (SHR) show ventricular dilation, changes in CSF proteins and variations in the circumventricular organs (CVO) such as: the subcommissural organ (SCO), the subfornical organ (SFO) and the area postrema (AP) which are located in the walls of the third and fourth ventricles. On the other hand, p73 proteins are present in cells of the central nervous system (CNS) such as circumventricular structures and the neuroepithelium which are altered in ventricular dilation. The purpose of the present work is to study the TAp73 isoform expression in the circumventricular organs (CVO) and their variations in ventricular dilatation and arterial hypertension. Brains and cerebrospinal fluid (CSF) from control Wistar-Kyoto rats (WKY) and SHR were used. The paraffin sections containing the CVO were immunohistochemically proc-essed with anti-TAp73 and by western blot, p73 bands in the CSF and circumventricular organ extract were also identified. The western blot study showed bands marked with p73 in the CSF and CVO, the p73 band expression was bigger in the SHR than in the WKY rats. We also found stronger markings in the SFO, SCO and AP of the hypertensive rats than in the WKY rats. It could be concluded that hypertension in the SHR produces altera-tions in the relationship between the p73 protein, circumventricular structures and CSF.
High Blood Pressure Effects on the Brain Barriers and Choroid Plexus Secretion  [PDF]
Ibrahim González-Marrero, Leandro Castaeyra-Ruiz, Juan M. González-Toledo, Agustín Castaeyra-Ruiz, Héctor de Paz-Carmona, Lidia Ruiz-Mayor, Agustín Castaeyra-Perdomo, Emilia M. Carmona-Calero
Neuroscience & Medicine (NM) , 2012, DOI: 10.4236/nm.2012.31009
Abstract: High blood pressure produces ventricular dilation, variations in circumventricular organs and changes in the cerebrospinal fluid compositions. On the other hand, chronic hypertension in spontaneously hypertensive rats can cause changes in the integrity of the brain barriers: blood-cerebrospinal fluid barrier and blood brain barrier. The permeability of the brain barriers can be studied by using transthyretin and S-100β. In the present work we study the integrity of the brain barrier and the choroid plexus function variations in arterial hypertension. Control rats and spontaneously hypertensive rats were used and the choroid plexus were processed by immunohistochemistry with anti-transthyretin and anti-vasopressin. Western blot was also performed in cerebrospinal fluid, serum and choroid plexus using anti-S-100β, anti-transthyretin. The accumulation of transthyretin immunoreactive was bigger in spontaneously hypertensive rats with respect to the control. Vasopressin was also higher in spontaneously hypertensive rats with respect to the control. Western blot showed that transthyretin tetramer was higher in the spontaneously hypertensive rats than in the control rats. The expression of transthyretin monomer was lower in hypertensive rats than the control in the cerebrospinal fluid, the transthyretin monomer reaction in the blood was stronger in hypertensive than in control rats. Western blot for the S-100 β showed an increase in blood and cerebrospinal fluid of hypertensive rats. The high blood pressure produces a disruption of the blood brain barrier and blood to cerebrospinal fluid barrier that allows extravasations from the cerebrospinal fluid to the blood and from the blood to the cerebrospinal fluid.
High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats
Ibrahim González-Marrero,Leandro Castañeyra-Ruiz,Juan M. González-Toledo,Agustín Castañeyra-Ruiz
International Journal of Hypertension , 2013, DOI: 10.1155/2013/164653
Luteinizing Hormone-Releasing Hormone Distribution in the Anterior Hypothalamus of the Female Rats
Leandro Castaeyra-Ruiz,Ibrahim González-Marrero,Agustín Castaeyra-Ruiz,Juan M. González-Toledo,María Castaeyra-Ruiz,Héctor de Paz-Carmona,Agustín Castaeyra-Perdomo,Emilia M. Carmona-Calero
ISRN Anatomy , 2013, DOI: 10.5402/2013/870721
Abstract: Luteinizing hormone-releasing hormone (LHRH) neurons and fibers are located in the anteroventral hypothalamus, specifically in the preoptic medial area and the organum vasculosum of the lamina terminalis. Most luteinizing hormone-releasing hormone neurons project to the median eminence where they are secreted in the pituitary portal system in order to control the release of gonadotropin. The aim of this study is to provide, using immunohistochemistry and female brain rats, a new description of the luteinizing hormone-releasing hormone fibers and neuron localization in the anterior hypothalamus. The greatest amount of the LHRH immunoreactive material was found in the organum vasculosum of the lamina terminalis that is located around the anterior region of the third ventricle. The intensity of the reaction of LHRH immunoreactive material decreases from cephalic to caudal localization; therefore, the greatest immunoreaction is in the organum vasculosum of the lamina terminalis, followed by the dorsomedial preoptic area, the ventromedial preoptic area, and finally the ventrolateral medial preoptic area, and in fibers surrounding the suprachiasmatic nucleus and subependymal layer on the floor of the third ventricle where the least amount immunoreactive material is found. 1. Introduction The luteinizing hormone-releasing hormone (LHRH) is a gonadotropin releasing hormone (GnRH), which acts on the pituitary hormones as a follicle stimulating hormone (FSH) and luteinizing hormone (LH), which act on the gonads, [1]. The GnRH neurons are originated in the nasal epithelium and migrate accompanying the fibers of the vomeronasal and terminal nerves [2, 3] up to the anterobasal part of the brain, where they enter the brain together with nerve terminals and then move caudally to the preoptic hypothalamus, where GnRH neurons are definitively located [2, 4]. These GnRH neurons and fibers are mainly located in the anteroventral third ventricle region, specifically in the preoptic area (PA) and the organum vasculosum of the lamina terminalis (OVLT) [5]. The anterior hypothalamus is the major region of the diencephalon implicated in the development of the olfactory system and the sexual differentiation of the brain. Most of the GnRH neurons axons project to the external zone of the median eminence where is GnRH secreted into the pituitary portal vasculature to control the release of gonadotropin [6–8]. The preoptic area (PA) is part of the anterior hypothalamus and is confined to the anteroventral region of the third ventricle (AV3V); the PA is divided into, the medial
Angiotensin II, Vasopressin, and Collagen-IV Expression in the Subfornical Organ in a Case of Syndrome of Inappropriate ADH
Emilia M. Carmona-Calero,Juan M. González-Toledo,Leandro Castaeyra-Ruiz,Ibrahim González-Marrero,María Castaeyra-Ruiz,Héctor de Paz-Carmona,Agustín Castaeyra-Ruiz,Nélida Rancel-Torres,Agustín Castaeyra-Perdomo
Advances in Endocrinology , 2014, DOI: 10.1155/2014/179795
Abstract: The syndrome of inappropriate antidiuretic hormone (SIADH) is a disease characterized by hyponatremia and hyperosmolarity of urine where vasopressin and angiotensin II are implicated in the alteration of salt water balance and cardiovascular and blood pressure regulation. The aim of this study is to analyse the expression of substances related with cardiovascular and salt water regulation in the subfornical organ in a case of SIADH. Two brains, one taken from a 66-year-old man with SIADH and the other from a 63-year-old man without SIADH, were used. Immunohistochemical study was performed using anti-angiotensin II, anti-vasopressin, and anti-collagen-VI as primary antibodies. Angiotensin and vasopressin immunoreaction were found in neurons, in perivascular spaces, and in the ependymal layer in the subfornical organ in both cases. However, in the SIADH case, the angiotensin II and collagen-IV expression in the SFO were different suggesting this organ’s possible participation in the physiopathology of SIADH. 1. Introduction Diabetes insipidus (DI), syndrome of inappropriate antidiuretic hormone (SIADH), and syndrome cerebral salt-wasting (CSW) are three pathologies with hyponatremia and hyperosmolarity and differentiation between them is important to prescribe the most appropriate treatments [1, 2]. SIADH is a disease which is characterized by the hyponatremia and hyperosmolarity of urine [3–5]. There are well known causes for this syndrome, such as neoplasmatic processes, disorders of the central nervous system, lung diseases, and the side effects of drugs. A study [6] of a large group of patients has revealed that SIADH occurs in 3% of patients with head and neck cancer, in 0.7% of patients with non-small-cell lung cancer, and in 15% of cases of small-cell lung cancer [6]. The standard therapy for SIADH is to treat the underlying malignant disease. If this is not possible or if the disease has become refractory, other treatment methods are available such as water restriction, demeclocycline therapy, or, in severe cases, infusion of hypertonic saline together with furosemide during treatment [6]. Total body water and tonicity are strictly regulated by the renal action of the antidiuretic hormone (ADH), renin-angiotensin-aldosterone system, and norepinephrine and by the thirst mechanism. Abnormalities in water balance are manifested in SIADH as sodium disturbances-hyponatremia and hypernatremia [6]. On the other hand, the presence of VAS, AGII, and TH and their implication in cardiovascular, salt water balance and blood pressure regulation have long been
High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats
Ibrahim González-Marrero,Leandro Castaeyra-Ruiz,Juan M. González-Toledo,Agustín Castaeyra-Ruiz,Hector de Paz-Carmona,Rafael Castro,Juan R. Hernandez-Fernaud,Agustín Castaeyra-Perdomo,Emilia M. Carmona-Calero
International Journal of Hypertension , 2013, DOI: 10.1155/2013/164653
Abstract: The aim of the present work is to analyze the cerebrospinal fluid proteomic profile, trying to find possible biomarkers of the effects of hypertension of the blood to CSF barrier disruption in the brain and their participation in the cholesterol and β-amyloid metabolism and inflammatory processes. Cerebrospinal fluid (CSF) is a system linked to the brain and its composition can be altered not only by encephalic disorder, but also by systemic diseases such as arterial hypertension, which produces alterations in the choroid plexus and cerebrospinal fluid protein composition. 2D gel electrophoresis in cerebrospinal fluid extracted from the cistern magna before sacrifice of hypertensive and control rats was performed. The results showed different proteomic profiles between SHR and WKY, that α-1-antitrypsin, apolipoprotein A1, albumin, immunoglobulin G, vitamin D binding protein, haptoglobin and α-1-macroglobulin were found to be up-regulated in SHR, and apolipoprotein E, transthyretin, α-2-HS-glycoprotein, transferrin, α-1β-glycoprotein, kininogen and carbonic anhidrase II were down-regulated in SHR. The conclusion made here is that hypertension in SHR produces important variations in cerebrospinal fluid proteins that could be due to a choroid plexus dysfunction and this fact supports the close connection between hypertension and blood to cerebrospinal fluid barrier disruption. 1. Introduction Cerebrospinal fluid is a functional system closely connected to the brain, and changes or variations in the CSF may mean an alteration in the brain expressed by encephalic disorders. However, the composition of CSF may also be altered by systemic diseases, such as arterial hypertension, and cerebral ventricular dilatation, changes in CSF protein, and variations of the choroid plexus and other circumventricular organs (CVO) have been described in spontaneously hypertensive rats (SHR) [1–5]. Therefore, SHR develop hydrocephalus and experimental studies explain that hydrocephalus induces alterations in CSF since there are disturbances, in the hydrocephalic brain, of oxidative metabolism and neurotransmission and perhaps damage to periventricular cells, particularly when intracranial pressure is elevated [6]. The sharp increase in systemic blood pressure only causes an acute increase in CSF pressure in normotensive animals and not in hypertensive patients [6]. The CSF pressure of SHR showed greater protection to the acute effects of phenylephrine than in control Wistar-Kyoto (WKY) rats, but a permeability increase of the blood to cerebrospinal fluid barrier to sucrose in
La ideología y su inserción / proyección en el discurso pedagógico no teórico
Revista de Pedagogía , 2004,
Abstract: the article presents a research in which the interdependent relationship or dialectical relationship between ideology and non-theoretical pedagogical discourses is established. this research has two main objectives; the first one is to explore and to approach as close as possible the concept of ideology; by studying certain classical and current authors who address this issue. the second objective is to observe how ideology projects itself into the field of pedagogical discourses, that is to say, to corroborate how ideology molds and orientates this kind of discourse. only a detailed and critical analysis based on previously established criteria can unveil the ideology underlying pedagogical discourses. especially, it is interesting to perceive how the author?s ideology is present in each of the enunciated statements, whether they intend to criticize or to make proposals. it is necessary to highlight that the acceptance of any given approach, perspective or position, implies a choice and a commitment which is ideological itself. a group of well-known authors in the field of language as a social vehicle and in the field of ideology are studied, all of which support our guiding study. a non-theoretical pedagogical discourse is understood as a type of human thought, coming from common sense, from live experience within the pedagogical field. it is a discourse of opinion, utilitarian, which values and interprets education and which expresses the great human concerns over the issues of education. it is the discourse originated in ordinary citizens and also in educators, who carry out their everyday pedagogical tasks and who observe or come up with concrete issues that need to be solved in the short term.
Photoacoustic Studies of Colloidal Silica Particles after MeV Ion-Induced Shape Deformation  [PDF]
Ulises Morales, Rosalba Casta?eda-Guzmán, Santiago Jesús Pérez-Ruiz, Juan Carlos Cheang Wong
New Journal of Glass and Ceramics (NJGC) , 2011, DOI: 10.4236/njgc.2011.12011
Abstract: Ordered arrays of colloidal submicrometer-sized silica particles deposited onto silicon wafers were irradiated with MeV Si ions. The spherical silica particles turned into oblate particles as a result of the increase of the particle dimension perpendicular to the ion beam direction and the decrease in the parallel direction. Pulsed laser photoacoustic spectroscopy was used to study the structural changes of the silica particles after the ion-induced shape deformation. Our purpose is to correlate the mechanical vibrations generated by the pulsed laser as a function of the Si irradiation parameters: ion energy and fluence. Fast Fourier transform analysis of the photoacoustic signal was carried out in order to obtain the normal vibration modes of the system. The size, size distribution and shape of the silica particles were determined by scanning electron microscopy. Our results revealed significant structural differences between the spherical and the deformed silica particles.
?Cómo abordar temas interdisciplinarios a partir de la Ecología Cultural?
Ruiz Torres, Mónica E.;Casta?eda, Xóchitl Salome;
Investigaciones geográficas , 2008,
Abstract: this article has as principal aim explain since from the cultural ecology interdisciplinary topics can be approached. for effects of the same one given two practical examples, the first one of them belongs to the production of flowers in the municipality of villa guerrero, whereas the second one will expose the social and ecological problems that they bring with the construction of hydraulics works in almoloya de juarez, both in the state of mexico.
Transformación de funciones: Marcos de coordinación y oposición de acuerdo con equivalencia
Ruiz Casta?eda,Dyanne; Gómez Becerra,Inmaculada;
Revista Latinoamericana de Psicología , 2010,
Abstract: this research is motivated by our interest in the difficulty that individuals show when trying to "change" or "convert" their behavioral patterns. recent research and theories developed inside the experimental analysis of behavior, specifically from a relational framework theory (rft), expose that phenomenon from a functional-contextual way. from this conceptual frame, the purpose of this paper is to establish the experimental history needed to develop sets of symmetry relational responses and equivalence in the frames of sameness, and show the way the functions are transformed into a frame of opposition based upon the consequences that individuals receive. in order to achieve this goal, a first study was designed and carried out. the results obtained from this study led to several variations in the procedure and a second experiment was held. the sample was composed by 16 participants, ages between 20 and 40 who had to execute a matching-to-sample task. 7 individuals were used for the first experiment (5 females and 2 males) and 9 for the second (6 females and 3 males). the task was composed by two phases and a final evaluation of the derived relations. the dependent variable was defined as the execution of the individual (number of trials) and the independent variable as the introduction of aversive and pleasant pictures. finally, the data obtained from both experiments is discussed intra e inter individual and some practical implications are outlined.
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