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Search Results: 1 - 10 of 4340 matches for " Lauree Ann Duprey-Reed "
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A Comparative Analysis of Vitamin D3 and Biofield Energy Treated Vitamin D3 in Bone Health Using MG-63 Cell Line
Lauree Ann Duprey-Reed, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
Orthoplastic Surgery & Orthopedic Care International Journal , 2018, DOI: 10.31031/OOIJ.2018.01.000525
Abstract: Poor bone health is related with various bone disease that predisposes to fractures, while sufficient supplementation with calcium and vitamin D suggested prevention of bone diseases. The present study aimed to find the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on in vitro bone health parameters using MG-63 cells such as alkaline phosphates enzyme (ALP) activity, collagen levels, and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lauree Ann Duprey-Reed and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability range 76% to 139% with safe and nontoxic profile of test samples on MG-63 cell line. ALP was significantly increased by 171.8% and 189.7% at 10μg/mL in the UT-DMEM BT-TI and BT-DMEM BT-TI groups, respectively as compared with the untreated group. In addition, 100% and 33.3% increased ALP at 0.1 and 10μg/mL, respectively in BT-DMEM UT-TI group as compared with the untreated group. The level of collagen was significantly increased at 10μg/mL by 145.8% in the UT-DMEM BT-TI group, while 65.8% and 145.8% at 1 and 10μg/mL, respectively in BT-DMEM UT-TI group as compared with the untreated group. In addition, BT-DMEM BT-TI group showed a significant increased collagen level by 86.3% and 136.1% at 1 and 10μg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 150%, 21%, and 73.2% at 0.1, 10, and 100μg/mL, respectively in the UT-DMEM BT-TI group, while 258.3% and 126.8% at 0.1 and 100μg/mL, respectively in the BT-DMEM UT-TI group as compared with the untreated group. In addition, BT-DMEM BT-TI group showed a significant increased bone mineralization by 33.3%, 32.7%, and 187.4% at 0.1, 10, and 100μg/mL, respectively as compared with the untreated group. Biofield Energy Treatment might be vital in promotion and maintenance of strong and healthy bones and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer, and many autoimmune disorders.
Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocytes After Incubation with Biofield Treated Herbomineral Formulation: Effect of Biofield Energy Healing Treatment - The Trivedi Effect
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Mayank Gangwar, Snehasis Jana
American Journal of Biomedical and Life Sciences , 2016, DOI: 10.11648/j.ajbls.20160406.12
Abstract: The use of herbomineral formulations in the healthcare sector has increased due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect) on the formulation when applied to splenocyte cells isolated from mice spleen. The formulation was divided into two parts; one was the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. The test formulation was evaluated to find the expression of pro-inflammatory cytokines such as TNF-α, MIP-1α, and IL-1β along with non-cytotoxic concentrations by MTT assay. The splenocytes were given the Biofield Energy Treated and untreated sample at concentrations range (0.00001053 to 10.53 μg/mL) for 48 hours and was reported with safe concentration up to 1.053 μg/mL with percentage viability range from 76.7% to 109.2% in both samples. Biofield Energy Healing significantly enhanced the cell viability as compared with the untreated formulation. The expression of TNF-α was significantly inhibited in the Biofield Treated formulation at 0.01053, 0.1053, and 1.053 μg/mL by 1.77%, 1.93%, and 3.55%, respectively compared with the untreated formulation. The rest of the tested concentrations of the Biofield Treated formulation showed an increase in TNF-α expression at 0.00001053, 0.0001053, and 0.001053 μg/mL by 7.26%, 8.50%, and 8.50%, respectively compared to the vehicle control group. Similarly, the MIP-1α expression was inhibited by the Biofield Energy Treated formulation and showed immunosuppression activity at 0.01053 μg/mL by 18.47% (p≤0.001) compared to the untreated formulation. MIP-1α expression was reported as 628.94 ± 13.0 pg/mL in the untreated formulation, while it decreased to 512.74 ± 1.9 pg/mL in the Biofield Treated formulation at 0.01053 μg/mL. In addition, the IL-1β secretion was also significantly inhibited by the Biofield Treated formulation at concentrations 0.001053, 0.01053, 0.1053, and 1.053 μg/mL by 72.02%, 50.16%, 30.68%, and 22.11%, respectively as compared with the untreated formulation. Overall, The Trivedi Effect? significantly down-regulated the pro-inflammatory cytokines and potentiated the immunosuppressive effect of the treated formulation, which can be better utilized in organ transplants, anti-aging, stress management, autoimmune disorders, and inflammatory disorders, etc. to modulate the immune system by improving overall health.
Evaluation of Structural Properties and Isotopic Abundance Ratio of Biofield Energy Treated (The Trivedi Effect) Magnesium Gluconate Using LC-MS and NMR
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana
European Journal of Biophysics , 2017, DOI: 10.11648/j.ejb.20170501.12
Abstract: The current research work was designed to explore the impact of The Trivedi Effect - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) by using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect- Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect Treated sample. The LC-MS analysis of the both control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 min with similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated sample was significantly increased by 34.33% compared with the control sample. Subsequently, the percentage change of the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was significantly decreased in the treated sample by 64.08% as compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly changed compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Biofield Energy Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors by using its kinetic isotope effects.
Evaluation of Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate Treated with Energy of Consciousness (The Trivedi Effect)
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana
Journal of Drug Design and Medicinal Chemistry , 2017, DOI: 10.11648/j.jddmc.20170301.12
Abstract: Magnesium gluconate is a classical organometallic salt used for the prevention and treatment of magnesium deficiency diseases. The objective of the current research was to explore the influence of The Trivedi Effect - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control, while another part was treated with The Trivedi Effect - Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The PXRD analysis exhibited significant alteration of the crystal morphology of the treated sample compared with the control sample. The crystallite size of the treated sample was remarkably changed from range -69.99% to 71.40% compared with the control sample. The average crystallite size was significantly decreased in the treated sample by 13.61% compared with the control sample. Particle size analysis revealed that the particle size in the treated sample at d10, d50, and d90 value was significantly decreased by 5.19%, 26.77%, and 18.22%, respectively compared with the control sample. The treated sample’s surface area was significantly enhanced (12.82%) compared with the control sample. The FT-IR and UV-vis analysis showed that the structure of the magnesium gluconate remained the same in both the treated and control samples. The TGA analysis revealed the four steps thermal degradation of the both samples and the total weight loss of the Biofield Energy Treated sample was increased by 0.55% compared with the control sample. The DSC analysis revealed that the melting temperature of the treated sample (171.72°C) was increased by 0.21% compared with the control sample (171.36°C). The latent heat of fusion was increased by 4.66% in the treated sample compared with the control sample. This result indicated that the thermal stability of treated sample was improved compared with the control sample. The current study infers that The Trivedi Effect - Biofield Energy Healing might lead to a new polymorphic form of magnesium gluconate, which would be more soluble, bioavailable, and thermally stable compared with the untreated compound. Hence, the treated magnesium gluconate would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging and other chronic infections.
An Impact of the Trivedi Effect - Biofield Energy Healing on Herbomineral Formulation for Immunomodulation of Pro-inflammatory Cytokines in Biofield Treated Mouse Splenocytes
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan JosephMahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Mayank Gangwar, Snehasis Jana
Advances in Biochemistry , 2016, DOI: 10.11648/j.ab.20160406.12
Abstract: Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect) on the test formulation when applied to splenocyte cells isolated from the Biofield Treated mice. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment. The other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. A wide concentration range (0.00001053 to 10.53 μg/mL) of the test formulation was used to determine non-cytotoxic concentrations using MTT assay. Further, the expression of pro-inflammatory cytokines (TNF-α, MIP-1α, and IL-1β) was determined by ELISA method. The test formulation was evaluated and found to be safe up to 1.053 μg/mL with a percentage cell viability range of 73% to 97% using MTT assay. The Biofield Treated formulation improved the cell viability up to 6.61% compared with the untreated test formulation. TNF-α expression was significantly inhibited by 16.72% at 0.1053 μg/mL compared with the untreated test formulation, however expression was significantly altered by 53.67% and 25.62% at 0.01053 and 1.053 μg/mL, respectively compared to the untreated test formulation. TNF-α expression was also suppressed in the Biofield Treated test formulation at 0.001053 and 0.1053 μg/mL by 4.0% and 8.56%, respectively as compared with the vehicle control. MIP-1α suppression was reported in the Biofield Treated test formulation at 0.00001053 to 1.053 μg/mL by 8.43%, 22.02%, 21.92%, 20.54%, 5.40%, and 19.82%, respectively compared with the vehicle control. However, the Biofield Treated formulation further exhibited substantial suppression of MIP-1α at 0.0001053, 0.001053, 0.01053, and 0.1053 μg/mL by 13.50%, 7.38%, 36.83% (p≤0.001), and 2.53%, respectively compared with the untreated test formulation. In addition, significant inhibition of IL-1β secretion was reported in the Biofield Treated formulation at 0.0001053, 0.001053, 0.01053, and 0.01053 μg/mL by 32.40%, 14.99%, 60.42%, and 15.15%, respectively compared with the untreated test formulation. The Biofield Energy Healing Treatment significantly potentiated the immunosuppressive effect of the test formulation in Biofield Treated mouse splenocytes, which can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
De patrias y muertes ya vencidas. Las mujeres y la revolución de los cubanos
Marlene Duprey
Memoria y Sociedad , 2008,
Abstract: Through the feminist debate proposed by constructivist theoretical currents, this essay suggests that the pronouncements of Cuban women who developed a critical reflection of the deplorable social conditions in Cuba before the 1959 socialist revolution, are similar to the criticisms that some women’s organizations are elaborating at the present time. This article shows that after 45 years of the Cuban Revolution, the social precarity of many women can be seen as a counter argument within Cuban society. The research for this article is based on the reading of primary sources such as the journal “Mujeres Cubanas”, speeches of Vilma Espín, and Ofelia Acosta in addition to memoires, speeches and other documents collected by Dra. Lynn Stoner. These documents are found in the Centro de Investigaciones Históricas at the Universidad de Puerto Rico, Rio Piedras.//A partir del debate feminista abierto por las corrientes teóricas de corte construccionista, este ensayo propone que los discursos de mujeres cubanas que elaboraron una crítica a las deplorables condiciones sociales de Cuban antes de la revolución socialista del 1959 son similares a la crítica que ciertos sectores de mujeres elaboran actualmente. Se alamos que, a poco más de cuarenta y cinco a os de la Revolución cubana, la precariedad social de muchas mujeres puede ser un referente para un contradiscurso dentro de la sociedad cubana. La investigación se centra en una lectura de fuentes de primera mano como la revista Mujeres cubanas, conferencia de Vilma Espín, Ofelia Acosta, y memorias, entre otros discursos y documentos recopilados por la doctora Lynn Stoner. Dicha colección Stoner se encuentra en el Centro de Investigaciones Históricas de la Universidad de Puerto Rico, Rio Piedras.
Evaluation of Mast Cells in Dermal versus Subcutaneous Hemangiomas and Hemangiosarcomas in Dogs  [PDF]
Katherine A. Barber, Kim M. Newkirk, Ann Reed, Robert L. Donnell
Open Journal of Veterinary Medicine (OJVM) , 2012, DOI: 10.4236/ojvm.2012.22010
Abstract: This study investigated the role of mast cells in canine cutaneous vascular tumors, and is the first such study to distinguish between tumors arising in the dermis versus the subcutis. Mast cell numbers in canine cutaneous hemangiomas (HA) and hemangiosarcomas (HSA) were evaluated to identify a relationship between mast cells, tumor type (HA, HSA), histologic location (dermis, subcutis) and tumor recurrence. One hundred and sixty-seven biopsies from 148 dogs were evaluated. Using only one biopsy from each dog, mast cell counts (MCC) for each tumor (n = 148) were obtained by averaging the number of mast cells counted in ten 400× fields. A significant difference in mean MCC was found only between tumor types, with HA having more mast cells than HSA (4.2 ± 4.2 vs. 2.2 ± 2.6; p < 0.001). No significant difference in mean MCC existed between tumors that recurred and those that did not. There was no difference in recurrence rate between tumor type or histologic location. Our results indicate that benign HA contain more mast cells than malignant endothelial cell tumors, regardless of histologic location; whether this is a cause or effect relationship remains to be determined.
Radioecology: Why Bother?  [PDF]
Emily Amanda Caffrey, Mary Elizabeth Leonard, Jonathan Bamberger Napier, Delvan Reed Neville, Kathryn Ann Higley
Journal of Environmental Protection (JEP) , 2014, DOI: 10.4236/jep.2014.53022
Abstract:

The importance of radioecology to the discussion of radioactive contamination is discussed here. This paper discusses the history of radioecology, alongside the most recent developments in the science. It describes the need for more environmental data, and explains where the gaps in current knowledge lie. The calculation of radiation dose to wildlife along with the complications in performing such calculations is discussed. The paper also tackles the difficult question of the implications of radioecology on nuclear waste management and site decommissioning policies. From the beginnings of the science to today, radioecology is poised to be an important field of study as humans continue to rely on ionizing radiation to improve their lives.

Type I interferon pathway in adult and juvenile dermatomyositis
Emily C Baechler, Hatice Bilgic, Ann M Reed
Arthritis Research & Therapy , 2011, DOI: 10.1186/ar3531
Abstract: Gene expression profiling of peripheral blood or affected tissues in patients with autoimmune diseases has revealed important insights into the molecular pathways underlying autoimmunity [1]. Several groups have used gene expression profiling in an attempt to uncover clues to the pathogenesis of dermatomyositis (DM) (Table 1). The most prominent and consistent finding of these studies has been the presence of a gene signature characteristic of type I interferon (IFN) pathway activation, discovered first in DM muscle tissue and later identified in peripheral blood cells.The identification of IFN occurred over 50 years ago when IFN was observed to be produced in response to viral illnesses. More recently, type I IFNs have been identified as an important mediator in autoimmune diseases including juvenile dermatomyositis (JDM) and adult dermatomyositis (ADM). There are at least three classes of IFNs, including what are referred to as type I IFNs, which in humans include 13 subtypes of IFNα, IFNβ, IFNω, IFNε, and IFNκ. All of the type I IFNs are thought to signal through the same receptor, type I IFN receptor. Other IFNs have been seen in autoimmune disorders, including DM, such as type II IFN (IFNγ) that is mainly produced by natural killer cells and activated T cells (T-helper type 1 cells), and type III IFNs that are a newly discovered class consisting of three members - IFNγ1, IFNγ2, and IFNγ3 - which have overlapping activities with type I IFNs but signal through a distinct receptor [2].Two microarray studies in limited numbers of DM patients provided the earliest evidence that type I IFN-inducible genes are upregulated in DM muscle tissue. Among the genetic risk factors for DM are HLA class II alleles. Over 85% of JDM patients are positive for DQA1*0501, as compared with only 25% of healthy controls [3]. In 2002 Tezak and colleagues described Affymetrix gene expression profiling of muscle biopsy tissue from four female Caucasian JDM patients, all of whom were DQA1*
Statistical analysis of real-time PCR data
Joshua S Yuan, Ann Reed, Feng Chen, C Neal Stewart
BMC Bioinformatics , 2006, DOI: 10.1186/1471-2105-7-85
Abstract: In the first approach, a multiple regression analysis model was developed to derive ΔΔCt from estimation of interaction of gene and treatment effects. In the second approach, an ANCOVA (analysis of covariance) model was proposed, and the ΔΔCt can be derived from analysis of effects of variables. The other two models involve calculation ΔCt followed by a two group t-test and non-parametric analogous Wilcoxon test. SAS programs were developed for all four models and data output for analysis of a sample set are presented. In addition, a data quality control model was developed and implemented using SAS.Practical statistical solutions with SAS programs were developed for real-time PCR data and a sample dataset was analyzed with the SAS programs. The analysis using the various models and programs yielded similar results. Data quality control and analysis procedures presented here provide statistical elements for the estimation of the relative expression of genes using real-time PCR.Real-time PCR is one of the most sensitive and reliably quantitative methods for gene expression analysis. It has been broadly applied to microarray verification, pathogen quantification, cancer quantification, transgenic copy number determination and drug therapy studies [1-4]. A PCR has three phases, exponential phase, linear phase and plateau phase as shown in Figure 1. The exponential phase is the earliest segment in the PCR, in which product increases exponentially since the reagents are not limited. The linear phase is characterized by a linear increase in product as PCR reagents become limited. The PCR will eventually reach the plateau phase during later cycles and the amount of product will not change because some reagents become depleted. Real-time PCR exploits the fact that the quantity of PCR products in exponential phase is in proportion to the quantity of initial template under ideal conditions [5,6]. During the exponential phase PCR product will ideally double during each cycle i
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