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Search Results: 1 - 10 of 139705 matches for " Lange K "
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Review: Richard A. Krueger & Mary Anne Casey (2000). Focus Groups. A Practical Guide for Applied Research (3. Auflage) Review: Richard A. Krueger & Mary Anne Casey (2000). Focus Groups. A Practical Guide for Applied Research (3rd edition) Rese a: Richard A. Krueger & Mary Anne Casey (2000). Focus Groups. A Practical Guide for Applied Research (tercera edición)
Jeffrey K. Lange
Forum : Qualitative Social Research , 2002,
Abstract: KRUEGER und CASEY haben ein Handbuch für die Planung, Vorbereitung und Durchführung von Fokusgruppen-Untersuchungen vorgelegt. Da die Autor(inn)en sich offensichtlich vor allem an Noviz(inn)en richten, mangelt es dem Buch an einer soliden theoretisch-methodologischen Grundlegung, und sie leisten damit teilweise dem Misstand Vorschub, dass anstelle gebr uchlicherer Termini idiosynkratische Ausdrucksweisen verwendet werden. Untersch tzt werden in dem Handbuch auch m gliche Vorteile von Fokusgruppen zum strukturierten Hervorrufen von "überraschung" und für das Aufdecken linguistischer Konstruktionen in den (Ziel-) Gruppen. URN: urn:nbn:de:0114-fqs0204280 KRUEGER and CASEY have prepared a handbook for planning, preparing for, and carrying out focus group studies. A work apparently aimed at an audience of novices, Focus Groups lacks solid theoretical grounding, promotes substituting idiosyncratic phrasing for technical terms more commonly used in the field, and overlooks or minimizes focus group advantages for evoking structured surprise and revealing target-audiences' linguistic constructions. URN: urn:nbn:de:0114-fqs0204280 KRUEGER y CASEY han presentado un manual para la planificación, preparación y realización de investigaciones sobre grupos focales. El libro carece de sólidos fundamentos teóricos metodológicos debido a que los autores se dirigen evidentemente ante todo a principiantes, y así favorecen en parte al inconveniente de utilizar expresiones idiosincráticas en vez de términos técnicos. En el manual se desestiman también las ventajas que pueden tener los grupos focales para evocaciones estructuradas de "sorpresa" y para develar construcciones linguísticas en el grupo de objetivos. URN: urn:nbn:de:0114-fqs0204280
Einpluss des alters und des thioaminos?urengehalts im futter auf die reproduktionsleistung des kaninchens bei postpartaler insemination
W Schlolaut, K Lange, S Paufler
Genetics Selection Evolution , 1980, DOI: 10.1186/1297-9686-12-3-301c
Abstract:
Einpluss des alters und des thioaminos urengehalts im futter auf die reproduktionsleistung des kaninchens bei postpartaler insemination
Schlolaut W,Lange K,Paufler S
Genetics Selection Evolution , 1980,
Abstract:
The exposure of cancer cells to hyperthermia, iron oxide nanoparticles, and mitomycin C influences membrane multidrug resistance protein expression levels
Franke K, Kettering M, Lange K, Kaiser WA, Hilger I
International Journal of Nanomedicine , 2013, DOI: http://dx.doi.org/10.2147/IJN.S37465
Abstract: sure of cancer cells to hyperthermia, iron oxide nanoparticles, and mitomycin C influences membrane multidrug resistance protein expression levels Original Research (1284) Total Article Views Authors: Franke K, Kettering M, Lange K, Kaiser WA, Hilger I Published Date January 2013 Volume 2013:8 Pages 351 - 363 DOI: http://dx.doi.org/10.2147/IJN.S37465 Received: 28 August 2012 Accepted: 27 October 2012 Published: 20 January 2013 Karolin Franke, Melanie Kettering, Kathleen Lange, Werner A Kaiser, Ingrid Hilger Institute of Diagnostic and Interventional Radiology, Department of Experimental Radiology, Institute of Diagnostic and Interventional Radiology I, University Hospital Jena, Friedrich-Schiller Universit t Jena, Jena, Germany Purpose: The presence of multidrug resistance-associated protein (MRP) in cancer cells is known to be responsible for many therapeutic failures in current oncological treatments. Here, we show that the combination of different effectors like hyperthermia, iron oxide nanoparticles, and chemotherapeutics influences expression of MRP 1 and 3 in an adenocarcinoma cell line. Methods: BT-474 cells were treated with magnetic nanoparticles (MNP; 1.5 to 150 μg Fe/cm2) or mitomycin C (up to 1.5 μg/cm2, 24 hours) in the presence or absence of hyperthermia (43°C, 15 to 120 minutes). Moreover, cells were also sequentially exposed to these effectors (MNP, hyperthermia, and mitomycin C). After cell harvesting, mRNA was extracted and analyzed via reverse transcription polymerase chain reaction. Additionally, membrane protein was isolated and analyzed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Results: When cells were exposed to the effectors alone or to combinations thereof, no effects on MRP 1 and 3 mRNA expression were observed. In contrast, membrane protein expression was influenced in a selective manner. The effects on MRP 3 expression were less pronounced compared with MRP 1. Treatment with mitomycin C decreased MRP expression at high concentrations and hyperthermia intensified these effects. In contrast, the presence of MNP only increased MRP 1 and 3 expression, and hyperthermia reversed these effects. When combining hyperthermia, magnetic nanoparticles, and mitomycin C, no further suppression of MRP expression was observed in comparison with the respective dual treatment modalities. Discussion: The different MRP 1 and 3 expression levels are not associated with de novo mRNA expression, but rather with an altered translocation of MRP 1 and 3 to the cell membrane as a result of reactive oxygen species production, and with shifting of intracellular MRP storage pools, changes in membrane fluidity, etc, at the protein level. Our results could be used to develop new treatment strategies by repressing mechanisms that actively export drugs from the target cell, thereby improving the therapeutic outcome in oncology.
The exposure of cancer cells to hyperthermia, iron oxide nanoparticles, and mitomycin C influences membrane multidrug resistance protein expression levels
Franke K,Kettering M,Lange K,Kaiser WA
International Journal of Nanomedicine , 2013,
Abstract: Karolin Franke, Melanie Kettering, Kathleen Lange, Werner A Kaiser, Ingrid Hilger Institute of Diagnostic and Interventional Radiology, Department of Experimental Radiology, Institute of Diagnostic and Interventional Radiology I, University Hospital Jena, Friedrich-Schiller Universit t Jena, Jena, Germany Purpose: The presence of multidrug resistance-associated protein (MRP) in cancer cells is known to be responsible for many therapeutic failures in current oncological treatments. Here, we show that the combination of different effectors like hyperthermia, iron oxide nanoparticles, and chemotherapeutics influences expression of MRP 1 and 3 in an adenocarcinoma cell line. Methods: BT-474 cells were treated with magnetic nanoparticles (MNP; 1.5 to 150 μg Fe/cm2) or mitomycin C (up to 1.5 μg/cm2, 24 hours) in the presence or absence of hyperthermia (43°C, 15 to 120 minutes). Moreover, cells were also sequentially exposed to these effectors (MNP, hyperthermia, and mitomycin C). After cell harvesting, mRNA was extracted and analyzed via reverse transcription polymerase chain reaction. Additionally, membrane protein was isolated and analyzed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Results: When cells were exposed to the effectors alone or to combinations thereof, no effects on MRP 1 and 3 mRNA expression were observed. In contrast, membrane protein expression was influenced in a selective manner. The effects on MRP 3 expression were less pronounced compared with MRP 1. Treatment with mitomycin C decreased MRP expression at high concentrations and hyperthermia intensified these effects. In contrast, the presence of MNP only increased MRP 1 and 3 expression, and hyperthermia reversed these effects. When combining hyperthermia, magnetic nanoparticles, and mitomycin C, no further suppression of MRP expression was observed in comparison with the respective dual treatment modalities. Discussion: The different MRP 1 and 3 expression levels are not associated with de novo mRNA expression, but rather with an altered translocation of MRP 1 and 3 to the cell membrane as a result of reactive oxygen species production, and with shifting of intracellular MRP storage pools, changes in membrane fluidity, etc, at the protein level. Our results could be used to develop new treatment strategies by repressing mechanisms that actively export drugs from the target cell, thereby improving the therapeutic outcome in oncology. Keywords: magnetic nanoparticles, hyperthermia, chemotherapy, drugs, MDR, MRP, cancer, nanotechnology, iro
Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries
F. Cremonesi,K. Anderson,A. Lange-Consiglio
Veterinary Medicine International , 2010, DOI: 10.4061/2010/102591
Abstract: Two methods have been described to recover oocytes from equine follicles in excised ovaries: aspiration and scraping. Aim of this work was to develop an effective method for collecting equine oocytes using Tuohy needle and comparing this technique to aspiration and scraping, with or without tunica albuginea removal. This hollow hypodermic needle, usually employed for inserting epidural catheters, is designed with a slightly curved tip, shaped similar to a small curette. In unpeeled ovaries, the recovery rates of Tuohy needle group was higher (<.05) than in the 16 g needle aspiration and in the scraped ovaries (57% versus 36% and 47%) while the rate of cumulus-intact oocytes was higher than aspiration (46.9% versus 39.36%) but lower than scraping (46.97%) (<.001). In unpeeled ovaries there was significant difference in maturation rate of oocytes recovered by Tuohy needle in respect to peeled ovaries (58.54% versus 50.17%, resp.). Combination of aspiration and scraping by Tuohy needle allows a faster and reliable collection of oocytes suitable for horse IVM.
Lipophilic aroylhydrazone chelator HNTMB and its multiple effects on ovarian cancer cells
Kyu Kim, Thilo S Lange, Rakesh K Singh, Laurent Brard
BMC Cancer , 2010, DOI: 10.1186/1471-2407-10-72
Abstract: Cell viability was analyzed via MTS cytotoxicity assays and NCI60 cancer cell growth screens. Apoptotic events were monitored via Western Blot analysis, fluorescence microscopy and TUNEL assay. FACS analysis was carried out to study Cell Cycle regulation and detection of intracellular Reactive Oxygen Species (ROS)HNTMB displayed high cytotoxicity (IC50 200-400 nM) compared to previously developed AHC (oVtBBH, HNtBBH, StBBH/206, HNTh2H/315, HNI/311; IC50 0.8-6 μM) or cancer drug Deferoxamine, a hexadentate iron-chelator (IC50 12-25 μM). In a NCI60 cancer cell line screen HNTMB exhibited growth inhibitory effects with remarkable differences in specificity depending on the cell line studied (GI50 10 nM-2.4 μM). In SKOV-3 ovarian cancer cells HNTMB treatment led to chromatin fragmentation and activation of the extrinsic and intrinsic pathways of apoptosis with specific down-regulation of Bcl-2. HNTMB caused delayed cell cycle progression of SKOV-3 through G2/M phase arrest. HNTMB can chelate iron and copper of different oxidation states. Complexation with copper lead to high cytotoxicity via generation of reactive oxygen species (ROS) while treatment with iron complexes of the drug caused neither cytotoxicity nor increased ROS levels.The present report suggests that both, non-complexed HNTMB as a chelator of intracellular trace-metals as well as a cytotoxic HNTMB/copper complex may be developed as potential therapeutic drugs in the treatment of ovarian and other solid tumors.The current treatment of a variety of tumors, including ovarian cancer, relies on organometallic platinum compounds. Ovarian cancer is the leading cause of death from gynecologic malignancies and ranks second among newly diagnosed gynecological cancers in the United States [1,2]. Although most women will initially respond to cytoreductive surgery and adjuvant paclitaxel-based and platinum-based chemotherapy, the majority will experience disease recurrence. While re-treatment with a platinum-based ag
Disordered eating and menstrual patterns in female university netball players
L Havemann, Z De Lange, K Pieterse, HH Wright
South African Journal of Sports Medicine , 2011,
Abstract: Objective. The primary aim of this study was to determine the prevalence of disordered eating (DE) behaviour and menstrual disorders in a group of provincial-to-national level student netball players. The secondary aim was to examine the relationship between body composition, energy intake, DE and menstrual patterns in student netball players. Methods. Twenty-six white female netball players from a South African university volunteered to participate in this cross-sectional descriptive study. Height, weight and body composition were measured. Energy intake was assessed with 24-hour recalls and menstrual patterns were assessed with a menstrual history questionnaire. Players also completed an Eating Disorder Inventory (EDI) and an Eating Attitudes Test (EAT-26) to assess DE behaviour. Results. Collectively 14 players (54%) were identified with DE behaviour and scored above the designated cut-off score for the EAT-26 (≥20, N=3), the EDI Body Dissatisfaction subscale (≥14, N=7), the EDI Drive for Thinness subscale (≥15, N=3), and/or answered ‘Yes’ (N=8) to DE behavioural questions. Eight players (31%) reported menstrual irregularities during the past 12 months, of whom four (15%) also reported secondary amenorrhoea (absence of ≥3 consecutive menstrual cycles) during training. Five players (19%) presented with DE behaviour, menstrual irregularity and primary and/or secondary amenorrhoea. Reported energy intake was significantly lower in the players with menstrual irregularities and secondary amenorrhoea compared with the remaining players (p<0.05). Conclusions. Top female student netball players may have suboptimal energy intakes and suffer from DE behaviour, menstrual irregularities and secondary amenorrhoea. Players and coaches should be aware of these risks to avoid related health and performance consequences.
La question des détours dans le transport routier de marchandises
Sandra Lange,Helmut K?ll,Flavio V. Ruffini
Revue de Géographie Alpine , 2009, DOI: 10.4000/rga.179
Abstract: La question des détours dans le transport transalpin de marchandises sur route figure constamment parmi les priorités des politiques de circulation. La pléthore de critères relatifs à la définition d’un détour a donné naissance à des points de vue divergents au sein des différents pays alpins, rendant le débat d’autant plus complexe. Le présent article propose des critères relatifs à la définition d’un détour ainsi qu’une analyse des détours faits en 2004 par les poids lourds franchissant les Alpes autrichiennes et suisses. L’analyse des détours faits par les poids lourds tend à démontrer qu’ils n’empruntent que rarement les itinéraires les plus courts. Il est intéressant de souligner que très peu de détours sont faits par le col du Saint-Gothard. Mais suivant le mode de calcul choisi, jusqu’à 740 000 poids lourds sur 1 996 000 font un détour de plus de 60 km par le col de Brenner alors que 18,1 % des véhicules pourraient emprunter un itinéraire plus court en passant par le col du Saint-Gothard et 11,5 % en passant par le col du San Bernardino. En théorie, la déviation vers des itinéraires plus courts des véhicules qui font un détour de plus de 60 km générerait une hausse de la circulation de 38 % au col du Saint-Gothard et de 149 % au col du San Bernardino. Aux cols de Brenner et de Tauern, la circulation diminuerait de 31 % et 16 % respectivement. Detours of trans-alpine freight transport by road are a constant issue on the traffic-policy agenda. A plethora of criteria regarding the definition of detours leads to diverging opinions prevailing in the individual Alpine countries and serves to complicate the discussion even further. This paper presents criteria regarding the definition of traffic detours as well as an analysis of the detours taken by heavy goods vehicles (trucks) at the Austrian and Swiss Alpine crossings in the year 2004. The analysis of routes taken goes to show that heavy goods vehicles do often not take the shortest routes. One point of interest is that only a few detours are taken via the St. Gothard crossing. However, at the Brenner crossing, depending on the mode of computation, up to 740,000 of the 1,996,000 truck tours take a detour of more than 60 km via the Brenner crossing although 18.1% and 11.5% of all truck tours would have a shorter alternative route via the St. Gothard and the San Bernardino crossing, respectively. A theoretical transfer of detours of more than 60 km to the shortest routes would lead to an increase in traffic of 38% at the St. Gothard and 149% at the San Bernardino crossing. The amount of traffic at the
Detours of Trans-alpine Goods Transport by Road
Flavio V. Ruffini,Helmut K?ll,Sandra Lange
Revue de Géographie Alpine , 2009, DOI: 10.4000/rga.185
Abstract: La question des détours dans le transport transalpin de marchandises sur route figure constamment parmi les priorités des politiques de circulation. La pléthore de critères relatifs à la définition d’un détour a donné naissance à des points de vue divergents au sein des différents pays alpins, rendant le débat d’autant plus complexe. Le présent article propose des critères relatifs à la définition d’un détour ainsi qu’une analyse des détours faits en 2004 par les poids lourds franchissant les Alpes autrichiennes et suisses. L’analyse des détours faits par les poids lourds tend à démontrer qu’ils n’empruntent que rarement les itinéraires les plus courts. Il est intéressant de souligner que très peu de détours sont faits par le col du Saint-Gotthard. Mais suivant le mode de calcul choisi, jusqu’à 740 000 poids lourds sur 1 996 000 font un détour de plus de 60 km par le col de Brenner alors que 18,1 % des véhicules pourraient emprunter un itinéraire plus court en passant par le col du Saint-Gothard et 11,5 % en passant par le col du San Bernardino. En théorie, la déviation vers des itinéraires plus courts des véhicules qui font un détour de plus de 60 km générerait une hausse de la circulation de 38 % au col du Saint-Gothard et de 149 % au col du San Bernardino. Aux cols de Brenner et de Tauern, la circulation diminuerait de 31 % et 16 % respectivement. Detours of trans-alpine freight transport by road are a constant issue on the traffic-policy agenda. A plethora of criteria regarding the definition of detours leads to diverging opinions prevailing in the individual Alpine countries and serves to complicate the discussion even further. This paper presents criteria regarding the definition of traffic detours as well as an analysis of the detours taken by heavy goods vehicles (trucks) at the Austrian and Swiss Alpine crossings in the year 2004. The analysis of routes taken goes to show that heavy goods vehicles do often not take the shortest routes. One point of interest is that only a few detours are taken via the St. Gotthard crossing. However, at the Brenner crossing, depending on the mode of computation, up to 740,000 of the 1,996,000 truck tours take a detour of more than 60 km via the Brenner crossing although 18.1% and 11.5% of all truck tours would have a shorter alternative route via the St. Gotthard and the San Bernardino crossing, respectively. A theoretical transfer of detours of more than 60 km to the shortest routes would lead to an increase in traffic of 38% at the St. Gotthard and 149% at the San Bernardino crossing. The amount of traffic at
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