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Search Results: 1 - 10 of 10325 matches for " Lan Qiao "
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Oil Spill Model Operational Application in Damage Assessment and Case Study of Validation  [PDF]
Bing Qiao, Feng Xiao, Ru Lan, Tao Li, Chunchao Li
Journal of Environmental Protection (JEP) , 2018, DOI: 10.4236/jep.2018.95033
Abstract: Oil spill modeling is an important technical measure to evaluate the impact of oil spills scientifically. Because of the great uncertainty in its early development, simulation results have not been used as the basis of judgments for environmental compensation cases. Despite this, scientific research institutes in many countries, including China, are still devoted to the research and development of oil spill models and their applications in environmental damage assessment, which makes it possible to apply them in the judicial arbitration of damages claims. The relevant regulations on the Chinese compensation fund for oil pollution damage from ships and the judicial authentication of environmental damage have also accredited such kind of modeling applications. In order to enhance the applicability of oil spill model further, it is necessary to expand its damage assessment function, and to test, calibrate and verify the accuracy of the evaluation. To this end, the author adopts the self-developed 3-dimentionaloil spill model—CWCM to simulate the “Tasman Sea” oil spill accident. By comparing the simulation results of tidal current field, wind field, oil spill trajectory with those observed, the model coding and parameter selection are corrected, and it is realized that the simulation being basically consistent with the measured results. In addition, the results of the scale reduced simulation test of oil spill weathering are applied verifying and perfecting the weathering model of CWCM. The technical requirements and process for operational application of oil spill model in judicial arbitration are also put forward. In view of the rapid simulation function, the operational updating program for oil spill weathering model, coupled current model and dynamic update wind field diagnostic model are put forward in order to further improve the operational evaluation function and evaluation efficiency of oil spill model.
Effective Linear Discriminant Analysis for High Dimensional, Low Sample Size Data
Zhihua Qiao,Lan Zhou,Jianhua Z. Huang
Lecture Notes in Engineering and Computer Science , 2008,
Sparse Linear Discriminant Analysis with Applications to High Dimensional Low Sample Size Data
Zhihua Qiao,Lan Zhou,Jianhua Z. Huang
IAENG International Journal of Applied Mathematics , 2009,
A Method to Improve the Embryogenesis Rate of Banana Somatic Cell Embryogenesis  [PDF]
Shenghe Chang, Zhengjing Wu, Wei Sun, Fei Lin, Qi Zeng, Jingyi Zhang, Lan Qiao, Haiyan Shu
American Journal of Plant Sciences (AJPS) , 2018, DOI: 10.4236/ajps.2018.93040
Abstract: Getting embryogenic callus is the first step for banana transformation. Enhancing embryonic-callus-rate is important for banana molecular breeding. In this paper, WIND1, an AP2/ERF transcription factor was added into the embryonic-callus-inducing medium with poly-Arg and nuclear localization signal (NLS). The embryogenic-callus-induced rate of the immature male florescence cultured on the media containing the recombinant protein Arg9-NLS-WIND1 was significantly higher than that of the immature male florescence cultured on the control media. Medium containing 0.01% of the protein Arg9-NLS-WIND1 had the best effect for inducing the embryogenesis of the immature male inflorescence. On the medium containing 0.01% of the protein Arg9-NLS-WIND1, 11.5% of the immature male flowers can develop embryonic callus. On the medium containing Arg9-NLS-WIND1, the protein might be transferred into cytoplasm of the explants by endocytosis due to the interaction between the poly-Arg transduction domain and the plasma membrane. After it entered the nucleus guided by NLS and bind with the target DNA domain, the somatic cell embryogenesis reactions were initiated and the embryonic callus formed.
Mercury Distribution in Tobacco (Nicotiana tabacum) Cell  [PDF]
Shenghe Chang, Zhengjing Wu, Wei Sun, Fei Lin, Jingyi Zhang, Qi Zeng, Lan Qiao, Haiyan Shu
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.94010
Abstract: Problem: Removing mercury from polluted soil using transgenic plants is an ideal method. However, where mercury was stored in plant cell is not clear until now. Methods: Differential centrifugation and laser scanning confocal microscopy were used in this study. Results and findings: Results showed that after mercury was absorbed by tobacco plants, most of the mercury accumulated in roots. Mercury content in root was significantly higher than that in shoot. In the seedlings cultured in MS liquid medium containing 5 μmol/L mercuric chloride, the mercury content in cell wall was 6.6 μg/g. The mercury content in cell membrane fraction was 2.1 μg/g. The mercury content in supernatant fraction was 35.1 μg/g. Most of the mercury accumulated in roots located in liquid fraction, about 15% of the mercury was attached by cell wall. Only a small part assembled in cell membrane. Most of the mercury in liquid fraction located in vacuole. This suggested that after the mercury was accumulated in plant root, most of the mercury was transferred into vacuole. There was no important cellular organ in vacuole. The toxicity of mercury in vacuole will be much lower than that in cell membrane or cellular organs. Recommendation
Factors Related to Physical Strength of Banana Pseudostem  [PDF]
Shenghe Chang, Zhengjing Wu, Wei Sun, Lan Qiao, Qi Zeng, Jingyi Zhang, Haiyan Shu
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.94011
Abstract: Banana is an important fruit in China. Banana production played important role in economic development in tropical region. Banana production in China was always cut because of lodging caused by typhoon. Getting new cultivars with high resistance to lodging is the basic resolution to resolve this problem. Screening and identifying the germ plasm resource is the first step to breed new cultivars. Banana plant height was high. A single banana plant needs large area. It is difficult to screen the germ plasm resource by identifying the physical strength of banana pseudostem. This research focused on studying the relationship between pseudostem and plant height, pseudostem diameter, acid soluble lignin, acid insoluble lignin, total lignin, pore numbers of pseudostem cross section, and the expression of 4-coumarate:CoA ligase (4CL). Results showed that the plant with high physical strength in seedling stage always has high physical strength in mature stage. The physical strength of banana seedling pseudostem was closely related to pseudostem diameter and total lignin. Pseudostem diameter and total lignin can be used to predict the physical strength of mature banana pseudostem. Work on identifying and screening the physical strength of banana germ plasm pseudostem can be reduced by measuring seedling pseudostem diameter and total lignin in pseudostem of banana germplasm.
A Method to Predict Banana Pseudostem’s Strength at Seedling Stage  [PDF]
Shenghe Chang, Zhengjing Wu, Wei Sun, Lan Qiao, Qi Zeng, Jingyi Zhang, Haiyan Shu
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.99027
Abstract: Banana industry played important roles in Chinese economics. Banana production was always affected heavily by typhoon. Selecting new cultivars with high strength was the basic way to resolve this problem. If the selecting work was performed at mature stage, it will need large area and much labor. If this work can be done at seedling stage, this process will be simplified significantly. We found that banana plants that had high strength at seedling stage always had high strength at mature stage. The physical strength of seedling pseudostem was closely related with pseudostem diameter, lignin content and the expression of 4-coumarate:CoA ligase gene Mu4CL15. Those seedlings whose pseudostem had bigger diameter, more lignin content and higher expression of Mu4CL15 always had higher strength to resist lodging. Through screening plants whose pseudostem had bigger diameter, more lignin content and higher expression of Mu4CL15, plants with high-strength pseudostem can be selected. Using this method, thousands of seedlings can be identified in short time. This process does not need large area and much labor. If this method was applied, the process of breeding new cultivars of banana with high resistance to lodging will be accelerated.

Qiao Lan,Cai Meifeng,

岩石力学与工程学报 , 1995,
Abstract: The application of stress relief method by overcoring is introduced for determinationof in-situ stresses in a gold mine.A series of new techniques are used in the measurement,such as the technique of complete temperature compensation,improved hollow inclusioncells,automatic data recording equipment and dual iteration method for determination ofrock properties and 3-D stress state at every measuring point.These new techniques ensure the reliability and accuracy of the measurement effectively.The measured insitustress state of the mine shows a good accordance with the regional tectonic stress field,which strongly proves the correctness of the measured results.
Akt2 Regulates Metastatic Potential in Neuroblastoma
Jingbo Qiao, Sora Lee, Pritha Paul, Lan Qiao, Chase J. Taylor, Cameron Schlegel, Nadja C. Colon, Dai H. Chung
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0056382
Abstract: Activation of PI3K/AKT pathway correlates with poor prognosis in patients with neuroblastoma. Our previous studies have demonstrated that PI3K/AKT signaling is critical for the oncogenic transformations induced by gastrin-releasing peptide (GRP) and its receptor, GRP-R, in neuroblastoma. Moreover, PI3K/AKT-dependent oncogenic transformations require N-myc, an extensively studied oncogene in neuroblastoma. Whether AKT directly regulates the expression of N-myc oncogene is yet to be determined. Here, we report a novel finding that of the three AKT isoforms, AKT2 specifically regulated N-myc expression in neuroblastoma cells. We also confirmed that GRP-R is upstream of AKT2 and in turn, regulated N-myc expression via AKT2 in neuroblastoma cells. Functional assays demonstrated that attenuation of AKT2 impaired cell proliferation and anchorage-independent cell growth, and decreased the secretion of angiogenic factor VEGF in vitro. Furthermore, silencing AKT2 inhibited migration and invasion of neuroblastoma cells in vitro. Xenografts established by injecting AKT2 silenced human neuroblastoma cells into murine spleen expressed decreased levels of AKT2 and resulted in fewer liver metastases compared to controls in vivo. Hence, our study highlights the potential molecular mechanism(s) mediating the oncogenic role of GRP/GRP-R and demonstrates a novel role for AKT2 in neuroblastoma tumorigenesis, indicating that targeting the GRP/GRP-R/AKT2 axis may be important for developing novel therapeutics in the treatment of clinically aggressive neuroblastoma.
Chiral Discrimination of Tryptophan Enantiomers via (1R, 2R)-2-Amino-1, 2-Diphenyl Ethanol Modified Interface
Juan Zhou,Qiao Chen,Li-lan Wang,Yong-hua Wang,Ying-zi Fu
International Journal of Electrochemistry , 2011, DOI: 10.4061/2011/502364
Abstract: The paper reported that a simple chiral selective interface constructed by (1R, 2R)-2-amino-1, 2-diphenyl ethanol had been developed to discriminate tryptophan enantiomers. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for the characteristic analysis of the electrode. The results indicated that the interface showed stable and sensitive property to determine the tryptophan enantiomers. Moreover, it exhibited the better stereoselectivity for L-tryptophan than that for D-tryptophan. The discrimination characteristics of the chiral selective interface for discriminating tryptophan enantiomers, including the response time, the effect of tryptophan enantiomers concentration, and the stability, were investigated in detail. In addition, the chiral selective interface was used to determine the enantiomeric composition of L- and D-tryptophan enantiomer mixtures by measuring the relative change of the peak current as well as in pure enantiomeric solutions. These results suggested that the chiral selective interface has the potential for enantiomeric discrimination of tryptophan enantiomers. 1. Introduction Chirality is an intrinsic property of most biological molecules. Chiral molecules may create a totally different effect on many biological processes in nature and life [1]. One enantiomer of drug may be more active or toxic than another isomer [2]. Due to the dramatic and total different effect of enantiomers compounds on human beings, chiral recognition is becoming a very active area in food, environment, clinic, medical, and pharmaceutical industry. Various techniques have been applied to discriminate and separate the racemic compounds [3–11]. Among these methods, electrochemistry with high sensitivity, simpleness, low cost, low-power requirements, and high compatibility has been a preferable approach for chiral recognition. One of the principal goals for chiral recognition is to construct an effectual chiral selective system, which should have recognition site for certain chiral enantiomers [12]. As one of amino alcohol derivatives, 2-amino-1, 2-diphenyl ethanol (ADE) is a chiral reagent having two asymmetric carbons in their structure. The amino group may recruit a negatively charged side chain and the hydroxy group may recruit hydrogen bonding donor/acceptor system. ADE enantiomers have been applied to prepare the chiral intermediates of bioactive molecule and have been used as a chiral ligand to induce asymmetric transformation [13, 14]. And it was a stuff to prepare chiral selector showing excellent enantioseparation
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