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Search Results: 1 - 4 of 4 matches for " LCA; "
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An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems
Gómez, GN;Nerli, BB;Acosta, OC;Picó, GA;Leiva, LCA;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2012, DOI: 10.1590/S1678-91992012000300008
Abstract: snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. we developed an alternative, simple and easy to scale-up method, able to purify simultaneously protease and phospholipase a2 toxins from bothrops alternatus venom. it comprises a multiple-step partition procedure with polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic step. two single bands in sds-polyacrylamide gel electrophoresis and increased proteolytic and phospholipase a2 specific activities evidence the homogeneity of the isolated proteins.
Heat tolerance of Nelore, Senepol x Nelore and Angus x Nelore heifers in the southeast region of Brazil
ARB Ribeiro, MM Alencar, AR Freitas, LCA Regitano, MCS Oliveira, MG Ibelli
South African Journal of Animal Science , 2010,
Abstract: The Brazilian beef industry has experienced an increase in the utilization of adapted and non-adapted Bos taurus breeds in crossbreeding systems. In spite of this, little is known about the adaptability of these groups and of their crossbred progeny when raised in a tropical climate. The aim of this study was to evaluate the physiological responses related to adaptability of Nelore (NE), crossbred Angus x Nelore (TA) and Senepol x Nelore (SN) cattle submitted to a heat tolerance trial. The study was conducted in the Southeast – Embrapa Cattle (CPPSE), S o Carlos, Brazil. During the summer of 2008, 45 heifers, 15 of each breed type, were evaluated over three days: at 7:00 a.m. (resting measure), at 13:00 (after six hours in the sun with no access to water and shade) and at 15:00 (after a further two hours in the sun with access to shade). Rectal temperature and sweating rate were measured and the data were analyzed using restricted maximum likelihood. The effect of breed type was significant for rectal temperature only at 15:00 and for the sweating rate at all three test periods. Based on these results, Senepol x Nelore heifers showed a better adaptation in the heat tolerance test than the other breed types.
Doxycycline-induced esophagitis: Report of two cases and review of the literature
Muhammed Sa??kara,Ya?ar Nazl?gül,Güler K?z?lca,Oktay Bulur
Dicle Medical Journal , 2010,
Abstract: Although it is not so frequent in medical literature, drug-induced esophageal injury is important, because of its possible complications. Here, we reported two patients with ulcerative esophagitis secondary to doxycycline use. Both patients applied to outpatient clinic with retrosternal pain, odynofagia and dysphagia. Symptoms had been developed after three days of doxicycline treatment in the first patient, and after two days in the second patient. Upper gastrointestinal endoscopy revealed ulcers in patient 1 at the upper 1/3 segment of esophagus and in patient 2 at the middle 1/3 segment of esophagus. Based on the endoscopic findings, the patients were diagnosed as drug-induced esophageal injury resulting from doxycycline treatments. Doxycycline treatments were stopped and lansoprazole and sucralfate was started.
Hydatid Disease Located in the Cerebellomedullary Cistern
?zgür K?z?lca,Murat Alta?,Utku ?enol,Murat Alp ?ztek
Case Reports in Medicine , 2014, DOI: 10.1155/2014/271365
Abstract: Hydatid disease is an endemic zoonotic disease in many areas of the world. Liver, followed by lung, is the most commonly affected organ and involvement of other organs is rare. When brain is involved, lesions are typically supratentorial, and infratentorial localisation is even rarer. We present a 45-year-old woman with hydatid disease located in premedullary location compressing the brain stem, an exceedingly rare location for cerebral echinococcosis. Relevant literature regarding typical properties of cerebral disease was reviewed. 1. Introduction Hydatid disease is endemic in many areas, especially in the Middle East, Turkey, South America, South Europe, New Zealand, and Australia [1–3]. The patients are usually asymptomatic or their symptoms are nonspecific since growth of the cysts is generally slow and therefore clinical manifestations tend to be nonspecific complaints due to compression of involved organs [2, 4]. The diagnosis depends on clinical suspicion, typically based on a history of living in, or having travelled to, an endemic area and contact with cattle or dogs and is confirmed with serologic tests and imaging [3]. The most common locations for hydatid cysts are the liver, followed by the lung [1, 2]. However, many parts of the body can be affected, including bones, pericardium, orbits, and brain [1, 5]. Cerebral localization is extremely rare, being seen in 2-3% of systemic disease and forming 2% of all intracranial space occupying lesions [2, 3, 5]. This rarity, coupled with nonspecific symptoms, necessitates a high degree of clinical suspicion and thus presents a diagnostic difficulty. We present a case of infratentorial cerebral hydatid disease with an exceedingly rare location, followed by a review of the literature regarding typical characteristics and imaging findings of cerebral echinococcosis. 2. Case A 45-year-old woman applied to a different center with complaints of nausea, vomiting, and headache that has been going on for three months. Following tests and examination, she was referred to our center with the initial diagnosis of a mass. Her physical examination revealed monoparesis in the left lower extremity and hypoesthesia on the left but was otherwise normal. Magnetic resonance imaging (MRI) in our center revealed an extra-axially located cystic lesion with a thin wall in the premedullary location compressing brain stem. The patient was operated and the lesion was revealed to be hydatid cyst (Figure 1). Figure 1: (a) T1W sagittal, (b) FLAIR transverse, and (c) postcontrast T1W transverse images. An unhanced cystic mass is
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