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Search Results: 1 - 10 of 341668 matches for " L.E.;Parada "
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A brain microdialysis study on 5-HT release in freely moving rat lines selectively bred for differential 5-HT1A receptor function
Gonzalez L.E.,Parada M.A.,Tucci S.,Teneud L.
Brazilian Journal of Medical and Biological Research , 2003,
Abstract: Breeding for high and low hypothermic responses to systemic administration of a serotonin1A (5-HT1A) receptor agonist (8-hydroxy-2-(di-n-propylamino)tetralin, 8-OH-DPAT) has resulted in high DPAT-sensitive (HDS) and low DPAT-sensitive (LDS) lines of rats, respectively. These lines also differ in several behavioral measures associated with stress. In the present microdialysis study we observed that basal 5-HT concentrations in the prefrontal cortex and dorsal hippocampus did not differ significantly between HDS and LDS rats. Thus, behavioral differences between the HDS and LDS lines might not be attributed to differences in basal 5-HT release. However, both lines had lower basal levels of 5-HT release than their randomly bred control group (random DPAT-sensitive, RDS) in the prefrontal cortex (mean ± SEM, pg/20 μl, was 3.0 ± 0.4 for LDS, 3.8 ± 0.3 for HDS and 6.4 ± 0.6 for RDS; F(2,59) = 5.8, P<0.005). The administration of (±)-fenfluramine (10 mg/kg) induced a greater increase in hippocampal 5-HT levels in HDS rats (500%) as compared with LDS (248%) or RDS (243%) rats (P<0.0001). There were no significant differences in the prefrontal cortex among lines, with a fenfluramine-induced 5-HT increase of about 900% in the three groups. This differential response to fenfluramine may be due to functional alterations of hippocampal 5-HT reuptake sites in the HDS line.
A brain microdialysis study on 5-HT release in freely moving rat lines selectively bred for differential 5-HT1A receptor function
Gonzalez, L.E.;Parada, M.A.;Tucci, S.;Teneud, L.;Overstreet, D.H.;Hernandez, L.;
Brazilian Journal of Medical and Biological Research , 2003, DOI: 10.1590/S0100-879X2003000200014
Abstract: breeding for high and low hypothermic responses to systemic administration of a serotonin1a (5-ht1a) receptor agonist (8-hydroxy-2-(di-n-propylamino)tetralin, 8-oh-dpat) has resulted in high dpat-sensitive (hds) and low dpat-sensitive (lds) lines of rats, respectively. these lines also differ in several behavioral measures associated with stress. in the present microdialysis study we observed that basal 5-ht concentrations in the prefrontal cortex and dorsal hippocampus did not differ significantly between hds and lds rats. thus, behavioral differences between the hds and lds lines might not be attributed to differences in basal 5-ht release. however, both lines had lower basal levels of 5-ht release than their randomly bred control group (random dpat-sensitive, rds) in the prefrontal cortex (mean ± sem, pg/20 μl, was 3.0 ± 0.4 for lds, 3.8 ± 0.3 for hds and 6.4 ± 0.6 for rds; f(2,59) = 5.8, p<0.005). the administration of (±)-fenfluramine (10 mg/kg) induced a greater increase in hippocampal 5-ht levels in hds rats (500%) as compared with lds (248%) or rds (243%) rats (p<0.0001). there were no significant differences in the prefrontal cortex among lines, with a fenfluramine-induced 5-ht increase of about 900% in the three groups. this differential response to fenfluramine may be due to functional alterations of hippocampal 5-ht reuptake sites in the hds line.
Efeito da adi??o de sulfito sobre a produ??o de alcoóis superiores durante a fermenta??o alcoólica
Gutierrez, L.E.;
Anais da Escola Superior de Agricultura Luiz de Queiroz , 1988, DOI: 10.1590/S0071-12761988000100022
Abstract: the effect of sulfited medium and its ph on the etanol, acetaldehyde and higher alcohols production during fermentation of cane molasse by saccharomyces cerevisiae was studied. there was no significant effect of sulfite on ethanol production. the results showed a significant increase in acetaldehyde and a reduction in higher alcohols content with the increase of sulfite concentration. the increase in the ph of sulfited medium caused an increase in acetaldehyde and higher alcohols content and did not affect ethanol production.
Efeito dos ácidos fórmico e propi?nico sobre a produ??o de alcoóis superiores durante a fermenta??o alcoólica
Gutierrez, L.E.;
Anais da Escola Superior de Agricultura Luiz de Queiroz , 1988, DOI: 10.1590/S0071-12761988000100023
Abstract: the effect of different concentrations of formic and propionic acids on the ethanol and higher alcohols production was studied with synthetic medium. there were no significant differences among ethanol , n-propilic, isobutilic and isoamilic alcohols in all treatments. however a reduction of isoamilic alcohol content was observed at higher levels of formic and propionic acids.
Acúmulo de trealose em linhagens de Saccharomyces durante fermenta??o alcoólica
Gutierrez, L.E.;
Anais da Escola Superior de Agricultura Luiz de Queiroz , 1990, DOI: 10.1590/S0071-12761990000200018
Abstract: this study was carried out to compare the effects of several factors (temperature, ph, sucrose concentration, 2,4-dinitrophenol and nitrogen sources) on trehalose production by saccharomyces uvarum iz-1904 and saccharomyces cerevisiae (m-300-a and baker's yeast) during alcoholic fermentation. trehalose production was lower with the yeast lz-1904 than with m-300-a or baker's yeast. more trehalose was formed at 34°c than at 25°c. for m-300-a and baker's yeast trehalose accumulation was higher at ph 4.5 than at ph 3.0. addition of 18ppm 2.4-dinitrophenol strongly decreased the amount of trehalose formed by baker's yeast, but was ineffective for iz-1904. increase of sucrose concentration led to a higher production of trehalose.
Efeito da adi??o de sulfato de am?nio sobre a produ??o de ácido succínico durante a fermenta??o alcoólica
Gutierrez, L.E.;
Anais da Escola Superior de Agricultura Luiz de Queiroz , 1988, DOI: 10.1590/S0071-12761988000100027
Abstract: succinic acid production by yeasts during alcoholic fermentation of cane molasse supplemented with 25, 50 and 100 ppm of nitrogen in the form of ammonium sulfate was determined by gas-liquid chromatography. ethanol production was not effected by the different levels of nitrogen, but there was a significant reduction in the content of succinic acid which was inversely related with the ammonium sulfate concentration in the medium.
Effect of some vitamins and micronutrient deficiencies on the production of higher alcohols by Saccharomyces cerevisiae
Gutierrez, L.E.;
Scientia Agricola , 1993, DOI: 10.1590/S0103-90161993000300024
Abstract: a study was carried out in order to determine the effect of vitamins (biotin, thiamine, pantotheniic acid and pyridoxal) and micronutrient (zinc, boron, manganese and iron) deficiencies on higher alcohol production during alcoholic fermentation with the industrially used yeast saccharomyces cerevisiae m-300-a. zinc deficiency induced a reduction on the levels of isobutyl and isoamyl alcohols. an increase on isobutyl alcohol (fivefold) and a reduction of isoamyl alcohol (two fold) and n-propyl alcohol (three fold) contents resulted from pantotheiiic acid deficiency, whereas pyridoxal deficiency caused an increase on the levels of isobutyl and isoamyl alcohols. biotin was not essential for the growth of this strain.
Changes in trehalose content of baker's yeast as affected by octanoic acid
Gutierrez, L.E.;
Scientia Agricola , 1993, DOI: 10.1590/S0103-90161993000300020
Abstract: octanoic acid inhibited ethanolic fermentation by saccharomyces cerevisiae (bakers yeast) and the trehalose accumulation, however did not affect the endogenous degradation of trehalose. this inhibition may be explained by the binding of octanoic acid to hexokinase or other proteins of plasma membrane because they are not necessary for endogenous fermentation. the degradation of trehalose may be due to an activation of trehalase.
Produ??o de álcoois superiores por linhagens de Saccharomyces durante a fermenta??o alcoólica
Gutierrez, L.E.;
Scientia Agricola , 1993, DOI: 10.1590/S0103-90161993000300021
Abstract: the production of higher alcohols by saccharomyces cerevisiae m-300-a, saccharomyces uvarum iz-1904 and baker's yeast (5. cerevisiae) was studied under several temperature conditions, sucrose level, ph, nitrogen sources and with 2-4 dinitrophenol (dnp). the yeast iz-1904 showed lower production of higher alcohols than other yeasts in all conditions studied. with the increase of temperature and higher level of sucrose an increase of isoamyl alcohol production was observed. a lower formation of higher alcohols was observed at ph 4.5 than at ph 3.0. with the addition of dnp occurred a significant reduction in isoamyl alcohol content. the yeasts did not show the sanie production of higher alcohols in relation to urea and ammonium sulfate.
Kayan phonemics.
L.E. Cubit
Bijdragen tot de Taal-, Land- en Volkenkunde , 1964,
Abstract:
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